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Protein

Heterogeneous nuclear ribonucleoprotein L

Gene

Hnrnpl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.By similarity

GO - Molecular functioni

  • mRNA 3'-UTR binding Source: RGD
  • mRNA CDS binding Source: RGD
  • nucleotide binding Source: InterPro
  • pre-mRNA intronic binding Source: UniProtKB
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  • cellular response to amino acid starvation Source: RGD
  • circadian rhythm Source: RGD
  • mRNA processing Source: InterPro
  • negative regulation of mRNA splicing, via spliceosome Source: RGD
  • positive regulation of mRNA binding Source: RGD
  • positive regulation of translation Source: RGD
  • regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  • response to peptide Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein L
Short name:
hnRNP L
Gene namesi
Name:HnrnplImported
Synonyms:Fblim1Imported
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi71059. Hnrnpl.

Subcellular locationi

  • Nucleusnucleoplasm By similarity
  • Cytoplasm 1 Publication

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. These granules are not identical with P bodies or stress granules.By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • intracellular ribonucleoprotein complex Source: UniProtKB
  • nucleoplasm Source: UniProtKB
  • nucleus Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • ribonucleoprotein granule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004360641 – 623Heterogeneous nuclear ribonucleoprotein LAdd BLAST623

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei98PhosphoserineBy similarity1
Cross-linki133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei182PhosphoserineBy similarity1
Modified residuei266N6-acetyllysineBy similarity1
Modified residuei288PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Modified residuei388Asymmetric dimethylarginineBy similarity1
Modified residuei392Asymmetric dimethylarginineBy similarity1
Modified residuei415PhosphoserineBy similarity1
Modified residuei578Phosphoserine; by CaMK4By similarity1

Post-translational modificationi

Several isoelectric forms of the L protein are probably the results of post-translational modifications.By similarity
Phosphorylation at Ser-578 by CaMK4 enhances interaction with a CaMK4-responsive RNA element (CaRRE1), and prevents inclusion of the stress axis-regulated exon (STREX) of the KCNMA1 potassium channel transcripts upon membrane depolarization.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiF1LQ48.
PRIDEiF1LQ48.

PTM databases

iPTMnetiF1LQ48.
PhosphoSitePlusiF1LQ48.

Expressioni

Gene expression databases

BgeeiENSRNOG00000020235.
ExpressionAtlasiF1LQ48. baseline and differential.

Interactioni

Subunit structurei

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with HNRNPLL. Interacts with APEX1; the interaction is DNA-dependent. Component of a complex with SETD2 (By similarity). Interacts with ELAVL1 (PubMed:18161049).By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027425.

Structurei

Secondary structure

1623
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi99 – 104Combined sources6
Helixi112 – 119Combined sources8
Helixi120 – 122Combined sources3
Beta strandi125 – 131Combined sources7
Turni132 – 135Combined sources4
Beta strandi136 – 143Combined sources8
Helixi144 – 148Combined sources5
Helixi151 – 154Combined sources4
Beta strandi159 – 170Combined sources12
Beta strandi172 – 174Combined sources3
Turni179 – 181Combined sources3
Helixi182 – 185Combined sources4
Beta strandi186 – 188Combined sources3
Beta strandi190 – 198Combined sources9
Helixi205 – 212Combined sources8
Helixi213 – 215Combined sources3
Beta strandi218 – 224Combined sources7
Beta strandi226 – 228Combined sources3
Beta strandi230 – 237Combined sources8
Helixi238 – 248Combined sources11
Beta strandi253 – 258Combined sources6
Beta strandi260 – 264Combined sources5
Beta strandi266 – 268Combined sources3
Beta strandi276 – 284Combined sources9
Helixi285 – 289Combined sources5
Beta strandi411 – 421Combined sources11
Turni425 – 427Combined sources3
Helixi430 – 437Combined sources8
Turni438 – 440Combined sources3
Beta strandi443 – 448Combined sources6
Beta strandi450 – 452Combined sources3
Beta strandi456 – 462Combined sources7
Helixi463 – 473Combined sources11
Beta strandi476 – 478Combined sources3
Beta strandi484 – 487Combined sources4
Beta strandi502 – 505Combined sources4
Beta strandi506 – 510Combined sources5
Helixi522 – 525Combined sources4
Beta strandi535 – 542Combined sources8
Helixi548 – 558Combined sources11
Beta strandi564 – 568Combined sources5
Beta strandi575 – 582Combined sources8
Helixi586 – 596Combined sources11
Beta strandi606 – 608Combined sources3
Beta strandi613 – 616Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MQLNMR-A86-190[»]
2MQMNMR-A174-291[»]
2MQNNMR-A408-623[»]
2MQONMR-A86-190[»]
2MQPNMR-A174-291[»]
2MQQNMR-A409-623[»]
4QPTX-ray1.35A409-623[»]
SMRiF1LQ48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 173RRM 1PROSITE-ProRule annotationAdd BLAST75
Domaini190 – 267RRM 2PROSITE-ProRule annotationAdd BLAST78
Domaini416 – 490RRM 3PROSITE-ProRule annotationAdd BLAST75
Domaini498 – 586RRM 41 PublicationAdd BLAST89

Domaini

RRM domain 2 has moderate RNA-binding affinity. RRM domains 3 and 4 may facilitate RNA looping when binding to two appropriately separated binding sites within the same target pre-mRNA.By similarity

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation1 Publication

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1456. Eukaryota.
ENOG410XQHN. LUCA.
GeneTreeiENSGT00550000074508.
HOGENOMiHOG000293298.
HOVERGENiHBG058843.
InParanoidiF1LQ48.
KOiK13159.
OMAiPYEGRRM.
OrthoDBiEOG091G0PR3.
TreeFamiTF354318.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 2 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: F1LQ48-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRRLLPRAE KRRRRLEQRQ QPDEQLRRAG AMVKMAAAGG GGGGGRYYGG
60 70 80 90 100
GNEGGRAPKR LKTENAGDQH GGGGGGGSGA AGGGGGENYD DPHKTPASPV
110 120 130 140 150
VHIRGLIDGV VEADLVEALQ EFGPISYVVV MPKKRQALVE FEDVLGACNA
160 170 180 190 200
VNYAADNQIY IAGHPAFVNY STSQKISRPG DSDDSRSVNS VLLFTILNPI
210 220 230 240 250
YSITTDVLYT ICNPCGPVQR IVIFRKNGVQ AMVEFDSVQS AQRAKASLNG
260 270 280 290 300
ADIYSGCCTL KIEYAKPTRL NVFKNDQDTW DYTNPNLSGQ GDPGSNPNKR
310 320 330 340 350
QRQPPLLGDH PAEYGEGRGF PSVDSRGSCA PARRPPRKFS PVLPLFPSHP
360 370 380 390 400
PGGPHGGYHS HYHDEGYGPP PPHYEGRRMG PPVGGHRRGP SRYGPQYGHP
410 420 430 440 450
PPPPPPPDYG PHADSPVLMV YGLDQSKMNC DRVFNVFCLY GNVEKVKFMK
460 470 480 490 500
SKPGAAMVEM ADGYAVDRAI THLNNNFMFG QKMNVCVSKQ PAIMPGQSYG
510 520 530 540 550
LEDGSCSYKD FSESRNNRFS TPEQAAKNRI QHPSNVLHFF NAPLEVTEEN
560 570 580 590 600
FFEICDELGV KRPTSVKVFS GKSERSSSGL LEWDSKSDAL ETLGFLNHYQ
610 620
MKNPNGPYPY TLKLCFSTAQ HAS
Length:623
Mass (Da):67,903
Last modified:April 3, 2013 - v2
Checksum:i0DEB312EC2B896AF
GO
Isoform 2 (identifier: F1LQ48-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-378: Missing.

Show »
Length:245
Mass (Da):27,251
Checksum:i4F814C3211650207
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0582471 – 378Missing in isoform 2. Add BLAST378

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB260892 mRNA. Translation: BAG72209.1.
AABR07002851 Genomic DNA. No translation available.
BC086392 mRNA. Translation: AAH86392.1.
RefSeqiNP_001128232.1. NM_001134760.1. [F1LQ48-1]
UniGeneiRn.2851.
Rn.33873.

Genome annotation databases

EnsembliENSRNOT00000027425; ENSRNOP00000027425; ENSRNOG00000020235. [F1LQ48-1]
ENSRNOT00000057034; ENSRNOP00000053867; ENSRNOG00000020235. [F1LQ48-2]
GeneIDi80846.
KEGGirno:80846.
UCSCiRGD:1359551. rat.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB260892 mRNA. Translation: BAG72209.1.
AABR07002851 Genomic DNA. No translation available.
BC086392 mRNA. Translation: AAH86392.1.
RefSeqiNP_001128232.1. NM_001134760.1. [F1LQ48-1]
UniGeneiRn.2851.
Rn.33873.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MQLNMR-A86-190[»]
2MQMNMR-A174-291[»]
2MQNNMR-A408-623[»]
2MQONMR-A86-190[»]
2MQPNMR-A174-291[»]
2MQQNMR-A409-623[»]
4QPTX-ray1.35A409-623[»]
SMRiF1LQ48.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027425.

PTM databases

iPTMnetiF1LQ48.
PhosphoSitePlusiF1LQ48.

Proteomic databases

PaxDbiF1LQ48.
PRIDEiF1LQ48.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027425; ENSRNOP00000027425; ENSRNOG00000020235. [F1LQ48-1]
ENSRNOT00000057034; ENSRNOP00000053867; ENSRNOG00000020235. [F1LQ48-2]
GeneIDi80846.
KEGGirno:80846.
UCSCiRGD:1359551. rat.

Organism-specific databases

CTDi3191.
RGDi71059. Hnrnpl.

Phylogenomic databases

eggNOGiKOG1456. Eukaryota.
ENOG410XQHN. LUCA.
GeneTreeiENSGT00550000074508.
HOGENOMiHOG000293298.
HOVERGENiHBG058843.
InParanoidiF1LQ48.
KOiK13159.
OMAiPYEGRRM.
OrthoDBiEOG091G0PR3.
TreeFamiTF354318.

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiF1LQ48.

Gene expression databases

BgeeiENSRNOG00000020235.
ExpressionAtlasiF1LQ48. baseline and differential.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 2 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHNRPL_RAT
AccessioniPrimary (citable) accession number: F1LQ48
Secondary accession number(s): F1LPP9, Q5U1Y5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 11, 2016
Last sequence update: April 3, 2013
Last modified: November 30, 2016
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.