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Protein

Misshapen-like kinase 1

Gene

Mink1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATPPROSITE-ProRule annotation
Active sitei153 – 1531Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • chemical synaptic transmission Source: UniProtKB
  • dendrite morphogenesis Source: UniProtKB
  • neuron cellular homeostasis Source: CACAO
  • protein autophosphorylation Source: UniProtKB
  • regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Misshapen-like kinase 1 (EC:2.7.11.1)
Alternative name(s):
GCK family kinase MiNK
MAPK/ERK kinase kinase kinase 6
Short name:
MEK kinase kinase 6
Short name:
MEKKK 6
Misshapen/NIK-related kinase
Mitogen-activated protein kinase kinase kinase kinase 6
Gene namesi
Name:Mink1
Synonyms:Map4k6, Mink
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1359135. Mink1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13361336Misshapen-like kinase 1PRO_0000413198Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei324 – 3241PhosphoserineBy similarity
Modified residuei326 – 3261PhosphoserineBy similarity
Modified residuei643 – 6431PhosphoserineCombined sources
Modified residuei703 – 7031PhosphoserineBy similarity
Modified residuei756 – 7561PhosphoserineBy similarity
Modified residuei765 – 7651PhosphoserineCombined sources
Modified residuei781 – 7811PhosphoserineBy similarity
Modified residuei782 – 7821PhosphoserineCombined sources
Modified residuei786 – 7861PhosphoserineCombined sources
Modified residuei895 – 8951PhosphothreonineBy similarity

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiF1LP90.

PTM databases

iPTMnetiF1LP90.
PhosphoSiteiF1LP90.

Expressioni

Developmental stagei

Increased expression from E18 to adulthood seen in cortex and hippocampus (at protein level).1 Publication

Interactioni

Subunit structurei

Interacts with RAP2A and NCK1 (By similarity). Interacts with TANC1.By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000050929.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 289265Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini1023 – 1310288CNHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni870 – 1336467Mediates interaction with RAP2ABy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi359 – 496138Gln-richAdd
BLAST
Compositional biasi543 – 731189Pro-richAdd
BLAST
Compositional biasi828 – 8314Poly-Ser
Compositional biasi838 – 8436Poly-Glu

Sequence similaritiesi

Contains 1 CNH domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0587. Eukaryota.
ENOG410XPHR. LUCA.
InParanoidiF1LP90.

Family and domain databases

InterProiIPR001180. CNH_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F1LP90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDPAPARQS DFIFLVALRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL
60 70 80 90 100
AAIKVMDVTE DEEEEIKQEI NMLKKYSHHR NIATYYGAFI KKSPPGNDDQ
110 120 130 140 150
LWLVMEFCGA GSVTDLVKNT KGNALKEDCI AYICREILRG LAHLHAHKVI
160 170 180 190 200
HRDIKGQNVL LTENAEVKLV DFGVSAQLDR TVGRRNTFIG TPYWMAPEVI
210 220 230 240 250
ACDENPDATY DYRSDIWSLG ITAIEMAEGA PPLCDMHPMR ALFLIPRNPP
260 270 280 290 300
PRLKSKKWSK KFTDFIDTCL IKTYLSRPPT EQLLKFPFIR DQPTERQVRI
310 320 330 340 350
QLKDHIDRSR KKRGEKEETE YEYSGSEEED DSHGEEGEPS SIMNVPGEST
360 370 380 390 400
LRREFLRLQQ ENKSNSEALK QQQQLQQQQQ RDPEAHIKHL LHQRQRRIEE
410 420 430 440 450
QKEERRRVEE QQRREREQRK LQEKEQQRRL EDMQALRREE ERRQAEREQE
460 470 480 490 500
YKRKQLEEQR QSERLQRQLQ QEHAYLNSQK QQQQQQQQQQ QQQQQQILPG
510 520 530 540 550
DRKPLYHYGR GINPADKPAW AREVEERARM NKQQNSPLAK TKPSSAGPEP
560 570 580 590 600
PIPQASPSPP GPLSQTPPMQ RPVEPQEGPH KSLVAHRVPL KPYAAPVPRS
610 620 630 640 650
QSLQDQPTRN LAAFPASHDP DPAAVPTPTA TPSARGAVIR QNSDPTSEGP
660 670 680 690 700
GPSPNPPSWV RPDNEAPPKV PQRTSSIATA LNTSGAGGSR PAQAVRARPR
710 720 730 740 750
SNSAWQIYLQ RRAERGTPKP PGPPAQPPGP PNTSSNPDLR RSDPGWERSD
760 770 780 790 800
SVLPASHGHL PQAGSLERNR NRVGASTKLD SSPVLSPGNK AKPEDHRSRP
810 820 830 840 850
GRPADFVLLK ERTLDEAPKP PKKAMDYSSS SEEVESSEDE EEEGDGEPSE
860 870 880 890 900
GSRDTPGGRS DGDTDSVSTM VVHDVEEVSG TQPSYGGGTM VVQRTPEEER
910 920 930 940 950
SLLLADSNGY TNLPDVVQPS HSPTENSQGQ SPPTKDGGGD YQSRGLVKAP
960 970 980 990 1000
GKSSFTMFVD LGIYQPGGSG DTIPITALVG GEGGRLDQLQ FDVRKGSVVN
1010 1020 1030 1040 1050
VNPTNTRAHS ETPEIRKYKK RFNSEILCAA LWGVNLLVGT ENGLMLLDRS
1060 1070 1080 1090 1100
GQGKVYGLIG RRRFQQMDVL EGLNLLITIS GKRNKLRVYY LSWLRNKILH
1110 1120 1130 1140 1150
NDPEVEKKQG WTTVGDMEGC GHYRVVKYER IKFLVIALKN SVEVYAWAPK
1160 1170 1180 1190 1200
PYHKFMAFKS FADLPHRPLL VDLTVEEGQR LKVIYGSSAG FHAVDVDSGN
1210 1220 1230 1240 1250
SYDIYIPVHI QSQITPHAIV FLPNTDGMEM LLCYEDEGVY VNTYGRIIKD
1260 1270 1280 1290 1300
VVLQWGEMPT SVAYICSNQI MGWGEKAIEI RSVETGHLDG VFMHKRAQRL
1310 1320 1330
KFLCERNDKV FFASVRSGGS SQVYFMTLNR NCIMNW
Length:1,336
Mass (Da):150,382
Last modified:October 19, 2011 - v2
Checksum:iA10289483BCD2F2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03073855 Genomic DNA. No translation available.
UniGeneiRn.32511.

Genome annotation databases

UCSCiRGD:1359135. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03073855 Genomic DNA. No translation available.
UniGeneiRn.32511.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000050929.

PTM databases

iPTMnetiF1LP90.
PhosphoSiteiF1LP90.

Proteomic databases

PaxDbiF1LP90.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:1359135. rat.

Organism-specific databases

RGDi1359135. Mink1.

Phylogenomic databases

eggNOGiKOG0587. Eukaryota.
ENOG410XPHR. LUCA.
InParanoidiF1LP90.

Miscellaneous databases

PROiF1LP90.

Family and domain databases

InterProiIPR001180. CNH_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMINK1_RAT
AccessioniPrimary (citable) accession number: F1LP90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 19, 2011
Last modified: September 7, 2016
This is version 39 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.