Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosine-protein kinase Yes

Gene

Yes1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis (By similarity).HMP:1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.IEP:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei303ATPIEP:1
Active sitei394Proton acceptorIEP:1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi281 – 289ATPIEP:9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • enzyme binding Source: RGD
  • epidermal growth factor receptor binding Source: RGD
  • ion channel binding Source: RGD
  • non-membrane spanning protein tyrosine kinase activity Source: GO_Central
  • phosphotyrosine residue binding Source: RGD
  • protein tyrosine kinase activity Source: RGD

GO - Biological processi

  • cell differentiation Source: GO_Central
  • cell migration Source: GO_Central
  • cellular response to platelet-derived growth factor stimulus Source: RGD
  • cellular response to retinoic acid Source: RGD
  • cellular response to transforming growth factor beta stimulus Source: RGD
  • glucose transport Source: RGD
  • innate immune response Source: GO_Central
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • positive regulation of peptidyl-tyrosine phosphorylation Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: RGD
  • protein autophosphorylation Source: RGD
  • regulation of cell proliferation Source: GO_Central
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-1433557. Signaling by SCF-KIT.
R-RNO-1433559. Regulation of KIT signaling.
R-RNO-2029481. FCGR activation.
R-RNO-389356. CD28 co-stimulation.
R-RNO-389513. CTLA4 inhibitory signaling.
R-RNO-3928662. EPHB-mediated forward signaling.
R-RNO-3928663. EPHA-mediated growth cone collapse.
R-RNO-3928665. EPH-ephrin mediated repulsion of cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Yes (EC:2.7.10.2)
Alternative name(s):
p61-Yes
Gene namesi
Name:Yes1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi3977. Yes1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedIEP:
ChainiPRO_00004138002 – 541Tyrosine-protein kinase YesAdd BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineIEP:1
Lipidationi3S-palmitoyl cysteine; in membrane formHMP:1
Modified residuei32PhosphotyrosineHMP:1
Modified residuei334PhosphotyrosineHMP:1
Modified residuei343PhosphotyrosineHMP:1
Modified residuei424Phosphotyrosine; by autocatalysisHMP:1
Modified residuei535PhosphotyrosineND:1

Post-translational modificationi

Phosphorylation by CSK on the C-terminal tail maintains the enzyme in an inactive state. Autophosphorylation at Tyr-424 maintains enzyme activity by blocking CSK-mediated inhibition (By similarity).HMP:
Palmitoylation at Cys-3 promotes membrane localization.HMP:

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiF1LM93.
PRIDEiF1LM93.

PTM databases

iPTMnetiF1LM93.

Expressioni

Gene expression databases

BgeeiENSRNOG00000037227.
ExpressionAtlasiF1LM93. baseline and differential.

Interactioni

Subunit structurei

Interacts with YAP1 and CSF1R (By similarity). Interacts with FASLG (By similarity). Interacts with CTNND1; this interaction allows YES1-mediated activation of FYN and FER and subsequent phosphorylation of CTNND1.HMP:1 Publication

GO - Molecular functioni

  • enzyme binding Source: RGD
  • epidermal growth factor receptor binding Source: RGD
  • ion channel binding Source: RGD
  • phosphotyrosine residue binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000053093.

Structurei

3D structure databases

SMRiF1LM93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini89 – 150SH3IEP:Add BLAST62
Domaini156 – 253SH2IEP:Add BLAST98
Domaini275 – 528Protein kinaseIEP:Add BLAST254

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118938.
InParanoidiF1LM93.
OMAiHVGHYGP.
OrthoDBiEOG091G0D46.
TreeFamiTF351634.

Family and domain databases

CDDicd12007. SH3_Yes. 1 hit.
Gene3Di3.30.505.10. 1 hit.
InterProiView protein in InterPro
IPR011009. Kinase-like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR036860. SH2_dom_sf.
IPR036028. SH3-like_dom_sf.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR035751. Yes_SH3.
PfamiView protein in Pfam
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiView protein in SMART
SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

F1LM93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCIKSKENK SPAIKYTPEN PTEPVNTSAG HYGVEHATAA TTSSTKGASA
60 70 80 90 100
NFNSLSMTPF GGSSGVTPFG GASSSFSVVP SSYPTSLTGG VTIFVALYDY
110 120 130 140 150
EARTTEDLSF KKGERFQIIN NTEGDWWEAR SIATGKNGYI PSNYVAPADS
160 170 180 190 200
IQAEEWYFGK MGRKDAERLL LNPGNQRGIF LVRESETTKG AYSLSIRDWD
210 220 230 240 250
EVRGDNVKHY KIRKLDNGGY YITTRAQFDT LQKLVKHYTE HADGLCHKLT
260 270 280 290 300
TVCPTVKPQT QGLAKDAWEI PRESLRLEVK LGQGCFGEVW MGTWNGTTKV
310 320 330 340 350
AIKTLKPGTM MPEAFLQEAQ IMKKLRHDKL VPLYAVVSEE PIYIVTEFMS
360 370 380 390 400
KGILLDFLKE GDGKYLKLPQ LVDMAAQIAD GMAYIERMNY IHRDLRAANI
410 420 430 440 450
LVGENLVCKI ADFGLARLIE DNEYTARQGA KFPIKWTAPE AALYGRFTIK
460 470 480 490 500
SDVWSFGILQ TELVTKGRVP YPGMVNREVL EQVERGYRMP CPQGCPESLH
510 520 530 540
ELMNLCWKKD PDERPTFEYI QSFLEDYFTA TEPQYQPGEN L
Length:541
Mass (Da):60,614
Last modified:May 3, 2011 - v1
Checksum:i3864A9ED1D3A3F2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03068393 Genomic DNA. No translation available.
AABR03068636 Genomic DNA. No translation available.
UniGeneiRn.214217.
Rn.6816.

Genome annotation databases

EnsembliENSRNOT00000056250; ENSRNOP00000053093; ENSRNOG00000037227.

Similar proteinsi

Entry informationi

Entry nameiYES_RAT
AccessioniPrimary (citable) accession number: F1LM93
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: May 3, 2011
Last modified: November 22, 2017
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome