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Protein

Probable dipeptidyl-aminopeptidase B

Gene

DAPB

Organism
Grosmannia clavigera (strain kw1407 / UAMH 11150) (Blue stain fungus) (Graphiocladiella clavigera)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Type IV dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.By similarity

Catalytic activityi

Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei826 – 8261Charge relay systemBy similarity
Active sitei903 – 9031Charge relay systemBy similarity
Active sitei936 – 9361Charge relay systemBy similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. serine-type peptidase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dipeptidyl-aminopeptidase B (EC:3.4.14.5)
Short name:
DPAP B
Gene namesi
Name:DAPB
ORF Names:CMQ_7762
OrganismiGrosmannia clavigera (strain kw1407 / UAMH 11150) (Blue stain fungus) (Graphiocladiella clavigera)
Taxonomic identifieri655863 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeOphiostomatalesOphiostomataceaeGrosmannia
ProteomesiUP000007796 Componenti: Unassembled WGS sequence

Subcellular locationi

Vacuole membrane By similarity; Single-pass type II membrane protein By similarity
Note: Lysosome-like vacuoles.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 125125CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei126 – 14621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini147 – 975829VacuolarSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 975975Probable dipeptidyl-aminopeptidase BPRO_0000412146Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi397 – 3971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi622 – 6221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi885 – 8851N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi496 – 5016Poly-Val
Compositional biasi661 – 6688Poly-Ala

Sequence similaritiesi

Belongs to the peptidase S9B family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiF0XS04.
OrthoDBiEOG72VHFG.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

F0XS04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEHGHNMWE EEPSKGRDSL DSDSSASTTS LVFDQISERV AAESGTTKGK
60 70 80 90 100
RMRYGHDEAD LGARLSELDD EDPLKDEASG DYDLETGPFL GGHGHNSNHD
110 120 130 140 150
GLSAASGSAG KTQGGYRMMD RGLRRVLIIA SLVFVTAWVG GLFIYISHKS
160 170 180 190 200
YLHGSEFEHD PQATVSRGSL RKITREQVDN GFWRPVKASI AWVAGPAGED
210 220 230 240 250
GLLLETNASG ASDKAYLTVQ DVRSLQQGLD ASTEAAVAAA RRTLVEKSTF
260 270 280 290 300
TFAGKTYRIG SSKASKDMSK VLLGVDVQSN WRHSSTAAYF ILEVATQTVQ
310 320 330 340 350
PLIPGEVSAR VQLAQWSPQS DAIAFTRDNN LYFRQVVAGS SSSAEDADSV
360 370 380 390 400
IKQITTDGGP ELFYGVPDWV YEEEVLGGAS ATWWSPDGRY IAFLRTNETG
410 420 430 440 450
VPEYPVQYFL HRPSGAAPAE GEENYPEVRQ IKYPKAGAHN PVVDLQFFDV
460 470 480 490 500
GRGDSFSVAV SGEFADENRL ITTVLWAGAQ KVLVKETNRV STVMRVVVVD
510 520 530 540 550
VAARSGQAVR TVDVGAIDGG WFEISQRTRF IPADPARQRP DDGYIDTIVH
560 570 580 590 600
NNGDHLAYFS PPENPEPIML TAGPDWEVDD APAAVDLERN LVYFLATIQG
610 620 630 640 650
ITQRHLYSVR LLDGGGLSPL TNTSEPGFYG ASFSAGVGAG YVLLEYGGPN
660 670 680 690 700
IPWQKVMNTP AAAAAAAAGS ADVSKQMPFV HVLEDNHELA ERARQYALPL
710 720 730 740 750
LVRGTFDVKG HDEGVGAGKL NYLERRPPHF DPSKKYPVLF QQYSGPGSQE
760 770 780 790 800
VTHEFSVDFQ SYVAASLGYV VVTVDPRGTG FAGRSNRVVV RGRLGVVESH
810 820 830 840 850
DHIAAAQHWA SLPYIDGDRL AIWGWSYGGF TTLKTLEQDA GRTFRYGIAV
860 870 880 890 900
APVTDWRFYD SVYTERYMDT PQANAVGYDT GAVTNASALA QNVRFLIMHG
910 920 930 940 950
IADDNVHLQN SLALLDRLDI EGVSNYDVHV FPDSDHSIYF HNGRQIVYDK
960 970
LENWLINAFN GEWLKIDNAK PQGKR
Length:975
Mass (Da):106,581
Last modified:May 2, 2011 - v1
Checksum:i487AC28233F1D863
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GL629990 Genomic DNA. Translation: EFW99394.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GL629990 Genomic DNA. Translation: EFW99394.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

InParanoidiF0XS04.
OrthoDBiEOG72VHFG.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: kw1407 / UAMH 11150.

Entry informationi

Entry nameiDAPB_GROCL
AccessioniPrimary (citable) accession number: F0XS04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2011
Last sequence update: May 2, 2011
Last modified: January 6, 2015
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.