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Protein

3-deoxy-D-manno-octulosonic acid transferase

Gene

waaA

Organism
Chlamydophila psittaci (strain ATCC VR-125 / 6BC) (Chlamydia psittaci)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of predominantly four 3-deoxy-D-manno-octulosonate (Kdo) residues from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A. Thus generates the genus-specific LPS epitope of Chlamydia, composed of the trisaccharide alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo.3 Publications

Catalytic activityi

Lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.2 Publications
Alpha-Kdo-(2->6)-lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP.2 Publications
Alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP.2 Publications
Alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->8)-(alpha-Kdo-(2->4))-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP.2 Publications

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei70Proton acceptorBy similarity1
Sitei141Transition state stabilizerBy similarity1
Sitei219Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15506.
BRENDAi2.4.99.12. 1312.
2.4.99.13. 1312.
2.4.99.14. 1312.
UniPathwayiUPA00958.

Protein family/group databases

CAZyiGT30. Glycosyltransferase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
3-deoxy-D-manno-octulosonic acid transferase (EC:2.4.99.122 Publications, EC:2.4.99.132 Publications, EC:2.4.99.142 Publications, EC:2.4.99.152 Publications)
Short name:
Kdo transferase
Alternative name(s):
Kdo(2)-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Kdo(3)-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Kdo-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Multifunctional Kdo transferase
Gene namesi
Name:waaA
Synonyms:gseA
Ordered Locus Names:CPSIT_0652, G5O_0645
OrganismiChlamydophila psittaci (strain ATCC VR-125 / 6BC) (Chlamydia psittaci)
Taxonomic identifieri331636 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei11 – 31Helical; Signal-anchorSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004191731 – 4333-deoxy-D-manno-octulosonic acid transferaseAdd BLAST433

Interactioni

Protein-protein interaction databases

STRINGi331636.CPSIT_0652.

Structurei

3D structure databases

ProteinModelPortaliF0T4D1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni277 – 278CMP-Kdo bindingBy similarity2
Regioni317 – 319CMP-Kdo bindingBy similarity3
Regioni344 – 347CMP-Kdo bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D8A. Bacteria.
COG1519. LUCA.
KOiK02527.
OMAiKHGGHNP.

Family and domain databases

InterProiIPR007507. Glycos_transf_N.
[Graphical view]
PfamiPF04413. Glycos_transf_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F0T4D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKGRRTKLH TFLYDCFLIF AFMVGLPRIL YKRFVHGKYT KSLGIRFGFK
60 70 80 90 100
KPEVPGTGPV AWFHGASVGE TALLLPLLKR FMKEYPEWRC VVTSCTESGH
110 120 130 140 150
ENAHRLFGPL GVTTFILPLD LSIIIKPVVR AISPSLLVFS EGDCWLNFIE
160 170 180 190 200
EAKRLGATAV IINGKLSANS CKRFTILKRF GRNYFSPVDG FLLQDEQHKA
210 220 230 240 250
RFLQLGVDKE KIQVTGNIKT YTETLSENNQ RDYWREKLQL AQDTELLVLG
260 270 280 290 300
SVHPKDVEVW LPVVRELRRN LKVLWVPRHI ERSKELEALL SKENISYGLW
310 320 330 340 350
SKEATFAQHD AIIVDAIGWL KQLYSAADLA FVGGTFDDRI GGHNLLEPLQ
360 370 380 390 400
CGVPLIFGPH IQSQSDLAER LLSMGAGCCL DKTNIVKVIT FLLDHPEERA
410 420 430
AYIQKGAMFL HEEKVAFDRT WESFKRYIPC VKI
Length:433
Mass (Da):49,322
Last modified:May 3, 2011 - v1
Checksum:i6DFD04C3A2885F0B
GO

Sequence cautioni

The sequence CAA49233 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69476 Genomic DNA. Translation: CAA49233.1. Different initiation.
CP002549 Genomic DNA. Translation: ADZ18468.1.
CP002586 Genomic DNA. Translation: AEB55626.1.

Genome annotation databases

EnsemblBacteriaiADZ18468; ADZ18468; CPSIT_0652.
AEB55626; AEB55626; G5O_0645.
KEGGichb:G5O_0645.
chp:CPSIT_0652.
PATRICi47105914. VBIChlPsi52874_0626.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69476 Genomic DNA. Translation: CAA49233.1. Different initiation.
CP002549 Genomic DNA. Translation: ADZ18468.1.
CP002586 Genomic DNA. Translation: AEB55626.1.

3D structure databases

ProteinModelPortaliF0T4D1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi331636.CPSIT_0652.

Protein family/group databases

CAZyiGT30. Glycosyltransferase Family 30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADZ18468; ADZ18468; CPSIT_0652.
AEB55626; AEB55626; G5O_0645.
KEGGichb:G5O_0645.
chp:CPSIT_0652.
PATRICi47105914. VBIChlPsi52874_0626.

Phylogenomic databases

eggNOGiENOG4105D8A. Bacteria.
COG1519. LUCA.
KOiK02527.
OMAiKHGGHNP.

Enzyme and pathway databases

UniPathwayiUPA00958.
BioCyciMetaCyc:MONOMER-15506.
BRENDAi2.4.99.12. 1312.
2.4.99.13. 1312.
2.4.99.14. 1312.

Family and domain databases

InterProiIPR007507. Glycos_transf_N.
[Graphical view]
PfamiPF04413. Glycos_transf_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKDTA_CHLP6
AccessioniPrimary (citable) accession number: F0T4D1
Secondary accession number(s): Q06380
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: May 3, 2011
Last modified: November 2, 2016
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.