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F0SDL3

- F0SDL3_PEDSD

UniProt

F0SDL3 - F0SDL3_PEDSD

Protein

4-hydroxy-tetrahydrodipicolinate synthase

Gene

dapA

Organism
Pedobacter saltans (strain ATCC 51119 / DSM 12145 / JCM 21818 / LMG 10337 / NBRC 100064 / NCIMB 13643)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 26 (01 Oct 2014)
      Sequence version 1 (03 May 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).UniRule annotationSAAS annotation

    Catalytic activityi

    Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei46 – 461Part of a proton relay during catalysisUniRule annotation
    Binding sitei47 – 471PyruvateUniRule annotation
    Sitei109 – 1091Part of a proton relay during catalysisUniRule annotation
    Active sitei135 – 1351Proton donor/acceptorUniRule annotation
    Active sitei164 – 1641Schiff-base intermediate with substrateUniRule annotation
    Binding sitei206 – 2061Pyruvate; via carbonyl oxygenUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-EC
    2. amine-lyase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
    2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    LyaseUniRule annotationSAAS annotationImported

    Keywords - Biological processi

    Amino-acid biosynthesis, Diaminopimelate biosynthesisUniRule annotationSAAS annotation, Lysine biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    Schiff baseUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciPSAL762903:GHB2-155-MONOMER.
    UniPathwayiUPA00034; UER00017.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxy-tetrahydrodipicolinate synthaseUniRule annotation (EC:4.3.3.7UniRule annotation)
    Short name:
    HTPA synthaseUniRule annotation
    Gene namesi
    Name:dapAUniRule annotation
    Ordered Locus Names:Pedsa_0154Imported
    OrganismiPedobacter saltans (strain ATCC 51119 / DSM 12145 / JCM 21818 / LMG 10337 / NBRC 100064 / NCIMB 13643)Imported
    Taxonomic identifieri762903 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesSphingobacteriiaSphingobacterialesSphingobacteriaceaePedobacter
    ProteomesiUP000000310: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919 PotentialImportedAdd
    BLAST
    Chaini20 – 292273 PotentialImportedPRO_5000720397Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer; dimer of dimers.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliF0SDL3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DapA family.UniRule annotation

    Keywords - Domaini

    SignalImported

    Phylogenomic databases

    KOiK01714.
    OMAiLADHENI.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00418. DapA.
    InterProiIPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001365. DHDPS. 1 hit.
    PRINTSiPR00146. DHPICSNTHASE.
    TIGRFAMsiTIGR00674. dapA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    F0SDL3-1 [UniParc]FASTAAdd to Basket

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    MSRFLGTGVA MVTPFQANGS IDFDGLAATI EHLINGKVEY IVVLGTTAES    50
    ATLSKDEKKQ VFKFVTERVN GRIPLVAGIG GNNTTEVVAE VNQFEMVGYE 100
    AILSVVPYYN KPTQEGIYQH YKSIAQAAKL PIILYNVPGR TGVSMSAETT 150
    IRLAREFKNI IATKEASGSF DQFNAIMADK PEGFELISGD DPITLPMVAL 200
    GAIGVISVVG NAFPVKFSEM VRLSLNGDFK AARPLHSSFL EFTRLCFVES 250
    NPCGVKAAMK ELGIIEDYVR LPLTPIGDST RAKVVAEVAK LK 292
    Length:292
    Mass (Da):31,574
    Last modified:May 3, 2011 - v1
    Checksum:i871A8139E06C7B61
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002545 Genomic DNA. Translation: ADY50740.1.
    RefSeqiWP_013631243.1. NC_015177.1.
    YP_004272562.1. NC_015177.1.

    Genome annotation databases

    EnsemblBacteriaiADY50740; ADY50740; Pedsa_0154.
    GeneIDi10229685.
    KEGGipsn:Pedsa_0154.
    PATRICi47000875. VBIPedSal163994_0162.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002545 Genomic DNA. Translation: ADY50740.1 .
    RefSeqi WP_013631243.1. NC_015177.1.
    YP_004272562.1. NC_015177.1.

    3D structure databases

    ProteinModelPortali F0SDL3.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADY50740 ; ADY50740 ; Pedsa_0154 .
    GeneIDi 10229685.
    KEGGi psn:Pedsa_0154.
    PATRICi 47000875. VBIPedSal163994_0162.

    Phylogenomic databases

    KOi K01714.
    OMAi LADHENI.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00017 .
    BioCyci PSAL762903:GHB2-155-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00418. DapA.
    InterProi IPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001365. DHDPS. 1 hit.
    PRINTSi PR00146. DHPICSNTHASE.
    TIGRFAMsi TIGR00674. dapA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51119 / DSM 12145 / JCM 21818 / LMG 10337 / NBRC 100064 / NCIMB 13643Imported.

    Entry informationi

    Entry nameiF0SDL3_PEDSD
    AccessioniPrimary (citable) accession number: F0SDL3
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 3, 2011
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 26 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Caution

    Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3