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Protein

4-hydroxy-tetrahydrodipicolinate synthase

Gene

dapA

Organism
Pseudopedobacter saltans (strain ATCC 51119 / DSM 12145 / JCM 21818 / LMG 10337 / NBRC 100064 / NCIMB 13643) (Pedobacter saltans)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).UniRule annotationSAAS annotation

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.UniRule annotation

Catalytic activityi

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotationSAAS annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 3 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (Pedsa_1481), Aspartokinase (Pedsa_0917)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei46Part of a proton relay during catalysisUniRule annotation1
Binding sitei47PyruvateUniRule annotation1
Sitei109Part of a proton relay during catalysisUniRule annotation1
Active sitei135Proton donor/acceptorUniRule annotation1
Active sitei164Schiff-base intermediate with substrateUniRule annotation1
Binding sitei206Pyruvate; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesisUniRule annotationSAAS annotation, Lysine biosynthesisUniRule annotationSAAS annotation
LigandSchiff baseUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciPSAL762903:G1GRS-156-MONOMER
UniPathwayiUPA00034; UER00017

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate synthaseUniRule annotationSAAS annotation (EC:4.3.3.7UniRule annotation)
Short name:
HTPA synthaseUniRule annotation
Gene namesi
Name:dapAUniRule annotation
Ordered Locus Names:Pedsa_0154Imported
OrganismiPseudopedobacter saltans (strain ATCC 51119 / DSM 12145 / JCM 21818 / LMG 10337 / NBRC 100064 / NCIMB 13643) (Pedobacter saltans)Imported
Taxonomic identifieri762903 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesSphingobacteriiaSphingobacterialesSphingobacteriaceaePseudopedobacter
Proteomesi
  • UP000000310 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi762903.Pedsa_0154

Structurei

3D structure databases

ProteinModelPortaliF0SDL3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DapA family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CDP Bacteria
COG0329 LUCA
KOiK01714
OMAiGMDACVP
OrthoDBiPOG091H00C1

Family and domain databases

CDDicd00950 DHDPS, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00418 DapA, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR005263 DapA
IPR002220 DapA-like
PANTHERiPTHR12128 PTHR12128, 1 hit
PfamiView protein in Pfam
PF00701 DHDPS, 1 hit
PIRSFiPIRSF001365 DHDPS, 1 hit
PRINTSiPR00146 DHPICSNTHASE
SMARTiView protein in SMART
SM01130 DHDPS, 1 hit
TIGRFAMsiTIGR00674 dapA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

F0SDL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRFLGTGVA MVTPFQANGS IDFDGLAATI EHLINGKVEY IVVLGTTAES
60 70 80 90 100
ATLSKDEKKQ VFKFVTERVN GRIPLVAGIG GNNTTEVVAE VNQFEMVGYE
110 120 130 140 150
AILSVVPYYN KPTQEGIYQH YKSIAQAAKL PIILYNVPGR TGVSMSAETT
160 170 180 190 200
IRLAREFKNI IATKEASGSF DQFNAIMADK PEGFELISGD DPITLPMVAL
210 220 230 240 250
GAIGVISVVG NAFPVKFSEM VRLSLNGDFK AARPLHSSFL EFTRLCFVES
260 270 280 290
NPCGVKAAMK ELGIIEDYVR LPLTPIGDST RAKVVAEVAK LK
Length:292
Mass (Da):31,574
Last modified:May 3, 2011 - v1
Checksum:i871A8139E06C7B61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002545 Genomic DNA Translation: ADY50740.1
RefSeqiWP_013631243.1, NC_015177.1

Genome annotation databases

EnsemblBacteriaiADY50740; ADY50740; Pedsa_0154
KEGGipsn:Pedsa_0154

Similar proteinsi

Entry informationi

Entry nameiF0SDL3_PSESL
AccessioniPrimary (citable) accession number: F0SDL3
Entry historyiIntegrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: March 28, 2018
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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