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F0S432 (F0S432_DESTD) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Dester_0965 EMBL ADY73604.1
OrganismDesulfurobacterium thermolithotrophum (strain DSM 11699 / BSA) [Complete proteome] [HAMAP] EMBL ADY73604.1
Taxonomic identifier868864 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesDesulfurobacteriaceaeDesulfurobacterium

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain99 – 290192ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding125 – 17652ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2441Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2571Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2571Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2591Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F0S432 [UniParc].

Last modified May 3, 2011. Version 1.
Checksum: 9FF052336F3CC109

FASTA29132,469
        10         20         30         40         50         60 
MKVAVIYGGP SPEAEISRKS AKSVIFALKK LGHQVFELEL DENITEKLKQ QKPDKVFITL 

        70         80         90        100        110        120 
HGSPGEDGTV QGLLEIAGYN YTGCNTQTSA ICMDKDITKR VLKTYNIPVP AGETFFQGDK 

       130        140        150        160        170        180 
IEPVEIPCVV KPARTGSTVG ISVVKDENSF QKAVEEAFKY DSKIIIEEYI EGREITVAVL 

       190        200        210        220        230        240 
NGFALPPVEI VTETGFYDFD SKYKKQTTEY KVPANLNLKL QEKLKKISEK IFKVLECKGA 

       250        260        270        280        290 
IRIDFRIDKW NNPYVLEVNT IPGMTERSLL PKAANAAGIP FEKLIEEILE S 

« Hide

References

[1]"The complete genome of Desulfurobacterium thermolithotrophum DSM 11699."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Pagani I., Ivanova N., Mikhailova N., Daligault H., Detter J.C., Tapia R., Han C., Land M., Hauser L., Markowitz V. expand/collapse author list , Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S., Brambilla E., Klenk H.-P., Eisen J.A.
Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 11699 / BSA.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002543 Genomic DNA. Translation: ADY73604.1.
RefSeqYP_004281663.1. NC_015185.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADY73604; ADY73604; Dester_0965.
GeneID10270201.
KEGGdte:Dester_0965.
PATRIC46942607. VBIDesThe167085_1002.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycDTHE868864:GHGF-993-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF0S432_DESTD
AccessionPrimary (citable) accession number: F0S432
Entry history
Integrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: February 19, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)