F0S2G1 (F0S2G1_DESTD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase B HAMAP-Rule MF_01080
EC=5.4.99.25 HAMAP-Rule MF_01080

Alternative name(s):
tRNA pseudouridine(55) synthase HAMAP-Rule MF_01080
tRNA pseudouridylate synthase HAMAP-Rule MF_01080
tRNA-uridine isomerase HAMAP-Rule MF_01080

Gene names

Name:	truB HAMAP-Rule MF_01080
Ordered Locus Names:	Dester_0378

Organism

Desulfurobacterium thermolithotrophum (strain DSM 11699 / BSA) [Complete proteome] [HAMAP] EMBL ADY73033.1

Taxonomic identifier

868864 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Aquificae › Aquificae › Aquificales › Desulfurobacteriaceae › Desulfurobacterium › Desulfurobacterium thermolithotrophum

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01080
Catalytic activity	tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01080 SAAS SAAS015240
Sequence similarities	Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. HAMAP-Rule MF_01080

Ontologies

Keywords
Biological process	tRNA processing HAMAP-Rule MF_01080 SAAS SAAS014780
Molecular function	Isomerase HAMAP-Rule MF_01080 SAAS SAAS014780
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	33	1	Nucleophile By similarity HAMAP-Rule MF_01080

Sequences

	Sequence		Length	Mass (Da)
	F0S2G1 [UniParc]. Last modified May 3, 2011. Version 1. Checksum: 6E20872CF9522CC2	FASTA	276	30,902
	10 20 30 40 50 60 MIDKPSGITS TEVVRVIKRF LGIKKIGHTG TLDPLATGLL ILAVGKATRF SEYLLKQDKC 70 80 90 100 110 120 YVVKGRFGLS SDTYDIDGNL KEVPCQEIKK EDLLKVLNNF KGEIEQVPPP YSAIRIKGKR 130 140 150 160 170 180 AYELARKGEK VELKPRKIKI YSLKLISFNY PDFELEVCCS SGTYIRSLVY DIGRALGCAA 190 200 210 220 230 240 VVTELRRTKV GKITIDKAIS LSKLNRENIK EFLIPIDKLL DMPEMKISID DGKKFRNGVK 250 260 270 IKVNALDGLY KVFSADKFLG VGIVKSNLLK PEKVLS

References

[1]

"The complete genome of Desulfurobacterium thermolithotrophum DSM 11699." US DOE Joint Genome Institute (JGI-PGF) Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Pagani I., Ivanova N., Mikhailova N., Daligault H., Detter J.C., Tapia R., Han C., Land M., Hauser L., Markowitz V. , Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S., Brambilla E., Klenk H.-P., Eisen J.A. Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 11699 / BSA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002543 Genomic DNA. Translation: ADY73033.1.
RefSeq	YP_004281092.1. NC_015185.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADY73033; ADY73033; Dester_0378.
GeneID	10269598.
KEGG	dte:Dester_0378.
PATRIC	46941350. VBIDesThe167085_0390.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K03177.
Enzyme and pathway databases
BioCyc	DTHE868864:GHGF-390-MONOMER.
Family and domain databases
HAMAP	MF_01080. TruB_bact.
InterPro	IPR002501. PsdUridine_synth. IPR020103. PsdUridine_synth_cat_dom. IPR014780. tRNA_psdUridine_synth_TruB. IPR015240. tRNA_sdUridine_synth_fam1_C. [Graphical view]
PANTHER	PTHR13767. PTHR13767. 1 hit.
Pfam	PF09157. TruB-C_2. 1 hit. PF01509. TruB_N. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00431. TruB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

F0S2G1_DESTD

Accession

Primary (citable) accession number: F0S2G1

Entry history

Integrated into UniProtKB/TrEMBL:	May 3, 2011
Last sequence update:	May 3, 2011
Last modified:	April 3, 2013
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)