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F0P4F5 (F0P4F5_STAPE) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:SPSE_0746 EMBL ADX76066.1
OrganismStaphylococcus pseudintermedius (strain ED99) [Complete proteome] [HAMAP] EMBL ADX76066.1
Taxonomic identifier984892 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain134 – 339206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding167 – 22256ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2931Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3061Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3061Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3081Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F0P4F5 [UniParc].

Last modified May 3, 2011. Version 1.
Checksum: B6C359A447DF0167

FASTA35639,892
        10         20         30         40         50         60 
MSKEALCIIY GGKSAEHDVS ILTAQNVLNA IDKARYHIDI IYITNDGQWK KMDNITEDIT 

        70         80         90        100        110        120 
DVEALRIDDV PTSDISAMLT QSSQGGRYDA VFPLLHGPNG EDGTIQGLFE VLDIPYVGNG 

       130        140        150        160        170        180 
VLAASSSMDK LVMKQLFAHR GLPQLPYVSF LRSEYEKYRK NIIKLVHDKL AFPVFVKPAN 

       190        200        210        220        230        240 
LGSSVGISKC EDEAALIKGI EEAFQFDRKL VIEQGVDARE IEVAVLGNDY PETTWPGEVV 

       250        260        270        280        290        300 
KDVAFYDYKS KYKDGKVKLA IPADLDQDVQ MTLRNMAVEA FKATDCSGLL RADFFVTADN 

       310        320        330        340        350 
QIYINETNAM PGFTAYSMYP KLWENMGVSY SELITKLIDL AKARYEDKQK NKHRID 

« Hide

References

[1]"Complete Genome Sequence of the Canine Pathogen Staphylococcus pseudintermedius."
Zakour N.L., Bannoehr J., van den Broek A.H., Thoday K.L., Fitzgerald J.R.
J. Bacteriol. 193:2363-2364(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ED99 EMBL ADX76066.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002478 Genomic DNA. Translation: ADX76066.1.
RefSeqYP_006014905.1. NC_017568.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADX76066; ADX76066; SPSE_0746.
GeneID12610508.
KEGGsdt:SPSE_0746.
PATRIC47203390. VBIStaPse182428_0705.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycSPSE984892:GLKW-733-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF0P4F5_STAPE
AccessionPrimary (citable) accession number: F0P4F5
Entry history
Integrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: June 11, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)