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F0NU85 (F0NU85_LACHH) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL ADX69382.1
Ordered Locus Names:LBHH_0146 EMBL ADX69382.1
OrganismLactobacillus helveticus (strain H10) [Complete proteome] [HAMAP] EMBL ADX69382.1
Taxonomic identifier767462 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain134 – 343210ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding169 – 22456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2971Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3101Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3101Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3121Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F0NU85 [UniParc].

Last modified May 3, 2011. Version 1.
Checksum: 14570A8C2E8A8E39

FASTA36040,333
        10         20         30         40         50         60 
MTKKTQVGLI FGGNSSEYEV SIISGRNIYK AIDKEKFDVH PIWITNEGYF ANEEESFKVL 

        70         80         90        100        110        120 
EDPSYQVKNP HKVHNISNLI ELENLPEIDV FFPIVHGNLG EDGVLQGLFR LMNKPFVGDD 

       130        140        150        160        170        180 
VLAAAVTMDK EFTKILAQRV GVPVADWIAI KRFEYDDKNN DKLDYEKVAE KLGRDMFVKP 

       190        200        210        220        230        240 
SNQGSSVGVS HVTNADEYAA ALKEAFKYDD KVLVEETVPG TEVETAVLGN DKPIVAGVGQ 

       250        260        270        280        290        300 
ITNAKGSFYS YKNKYDDNST SKLQIPADLP QEIIDTIRRN ARKVYEVTEC SGMARIDSML 

       310        320        330        340        350        360 
TPGGKVVLTE VNALPGFTNI SMYPKLFEEA GVPYTELITR LIEVGMERFD HKKTLLHKHD 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Lactobacillus helveticus strain H10."
Zhang H., Zhao W., Chen Y., Sun Z., Sun T., Meng H.
Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: H10.
[2]"Complete genome sequence of Lactobacillus helveticus H10."
Zhao W., Chen Y., Sun Z., Wang J., Zhou Z., Sun T., Wang L., Chen W., Zhang H.
J. Bacteriol. 193:2666-2667(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: H10 EMBL ADX69382.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002429 Genomic DNA. Translation: ADX69382.1.
RefSeqYP_005849369.1. NC_017467.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADX69382; ADX69382; LBHH_0146.
GeneID12457305.
KEGGlhl:LBHH_0146.
PATRIC47132618. VBILacHel162941_0165.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycLHEL767462:GLF7-153-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF0NU85_LACHH
AccessionPrimary (citable) accession number: F0NU85
Entry history
Integrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: June 11, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)