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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Thermococcus barophilus (strain DSM 11836 / MP)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei163Proton acceptorUniRule annotation1
Binding sitei165SubstrateUniRule annotation1
Metal bindingi189Magnesium; via carbamate groupUniRule annotation1
Metal bindingi191MagnesiumUniRule annotation1
Metal bindingi192MagnesiumUniRule annotation1
Active sitei281Proton acceptorUniRule annotation1
Binding sitei282SubstrateUniRule annotation1
Binding sitei314SubstrateUniRule annotation1
Sitei322Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:TERMP_00818Imported
OrganismiThermococcus barophilus (strain DSM 11836 / MP)Imported
Taxonomic identifieri391623 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000007478 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei189N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi391623.TERMP_00818.

Structurei

3D structure databases

ProteinModelPortaliF0LLL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 131RuBisCO_large_NInterPro annotationAdd BLAST120
Domaini143 – 438RuBisCO_largeInterPro annotationAdd BLAST296

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni367 – 369Substrate bindingUniRule annotation3
Regioni389 – 392Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
HOGENOMiHOG000230831.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

CDDicd08213. RuBisCO_large_III. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

Sequencei

Sequence statusi: Complete.

F0LLL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKFDKIYD YYVDKSYEPN KKRDIIAVFR ITPAEGYSIE AVAGGVAAES
60 70 80 90 100
STGTWTTLYN WYEEERWADL SAKAYDFHDM GDGSWIVKIA YPFHAFEEAN
110 120 130 140 150
LPGLLASIAG NIFGMRRAKA LRLEDIYFPE KLIREFDGPA FGMEGVRKML
160 170 180 190 200
EIKDRPIYGV VPKPKVGYSP EEFEKLATEL LMNGADYMKD DENLTSPWYN
210 220 230 240 250
RFEERAEIIA RIIEKVENET GEKKTWFANI TANVPEMEER LEILADLGLK
260 270 280 290 300
HAMVDVVITG WGALQYIRDL AADYGLAIHG HRAMHAAFTR NKYHGISMFV
310 320 330 340 350
LAKLYRLIGI DQLHVGTAGA GKLEGGKWDV IQNARIFRES HYKPDENDVF
360 370 380 390 400
HLEQKFYHIK AGFPTSSGGL HPGNLPIVFD ALGTDIVIQV GGGTLGHPDG
410 420 430 440
PAAGARAVRQ AIDAYMQGIP LDEYAKTHEE LARALEKWGH VTPV
Length:444
Mass (Da):49,883
Last modified:May 3, 2011 - v1
Checksum:i8912158B172E6588
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002372 Genomic DNA. Translation: ADT83795.1.
RefSeqiWP_013467093.1. NC_014804.1.

Genome annotation databases

EnsemblBacteriaiADT83795; ADT83795; TERMP_00818.
GeneIDi26136270.
KEGGitba:TERMP_00818.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002372 Genomic DNA. Translation: ADT83795.1.
RefSeqiWP_013467093.1. NC_014804.1.

3D structure databases

ProteinModelPortaliF0LLL8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391623.TERMP_00818.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADT83795; ADT83795; TERMP_00818.
GeneIDi26136270.
KEGGitba:TERMP_00818.

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
HOGENOMiHOG000230831.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

CDDicd08213. RuBisCO_large_III. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiF0LLL8_THEBM
AccessioniPrimary (citable) accession number: F0LLL8
Entry historyi
Integrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: November 30, 2016
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.