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F0L5E2

- F0L5E2_AGRSH

UniProt

F0L5E2 - F0L5E2_AGRSH

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Agrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3))
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 28 (01 Oct 2014)
      Sequence version 1 (03 May 2011)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    HydrolaseUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Ligandi

    MagnesiumUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciASP861208:GH59-335-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Gene namesi
    Name:glnDUniRule annotationImported
    Ordered Locus Names:AGROH133_03522Imported
    OrganismiAgrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3))Imported
    Taxonomic identifieri861208 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium
    ProteomesiUP000007455: Chromosome circular

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini523 – 60684HDUniRule annotationAdd
    BLAST
    Domaini730 – 81182ACT 1UniRule annotationAdd
    BLAST
    Domaini841 – 92080ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 373373UridylyltransferaseUniRule annotationAdd
    BLAST
    Regioni374 – 729356Uridylyl-removingUniRule annotationAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 1 HD domain.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains ACT domains.SAAS annotation
    Contains HD domain.SAAS annotation

    Keywords - Domaini

    RepeatUniRule annotationSAAS annotation

    Phylogenomic databases

    KOiK00990.
    OMAiLYCLWDM.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    F0L5E2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAIKDLDFSE ILDVSALKRE CDIIFKNDGK RIADVRSDLL PIFRKASTEG    50
    REKARELLKS DGSGIDCARR ISWLQDRLIE ILYDLACQYV YPKDAPQIAV 100
    AAVGGYGRGT LAPGSDIDLL FLLPAKNTPD MHKAVEFVLY LLWDLGFKVG 150
    HATRTVDECV RLSKSDMTIR TAILEVRAIC GKKSLTDDLE KRFESEVVTG 200
    TGPEFIAAKL AERDQRHRKA GDTRYLVEPN VKEGKGGLRD LHTLFWIAKY 250
    YYHVRDTADL VKLGVLSKRE LRLFEKADDF LWAVRCQMHF ITNKAEERLS 300
    FDIQREIADA LGYQPRPGLS AVERFMKHYF LVAKDVGDLT RIVCAALEDR 350
    QAKDVPGLSG VLSRFAHRVR KIPGSVEFVE DRGRIALAKP DVFKNDPVNL 400
    IRLFHIADIN NLELHPDALR VVTRSLSLIN DGLRENEEAN RLFLAILTSR 450
    RDPALTLRRM NEAGVLGKFI PEFGKIVAMM QFNMYHHYTV DEHLIRSVGV 500
    LSEVDKGTAV DAHPLANQLM PGVEEREALY VAVLLHDVAK GRQEDHSIAG 550
    ARVARKLCQR FRLTGKQTEM VVWLIEQHLL MSMVAQTRDL HDRKTITDFA 600
    DKVQSMERLK MLLILTVCDI RAVGPGVWNG WKGQLLRSLY YETELLLSGG 650
    FSEVSRKERA HIARQALYDA LEDWGQKARR KYTKLHYEPY LLTVALEDQV 700
    RHTRFIREAD KQEKALSTMV RTHSFHAITE ITVLAPDHPR LLSIITGACA 750
    AAGANIADAQ IFTTSDGRAL DTILINREFP IDEDETRRGN NVGKLIEEVL 800
    SGRQRLPEMI ATRTKSRRKK SAFTIPPSVI ISNGLSNKFT VIEVECLDRP 850
    GLLADMTAVI ADLSLDIHSA RITTFGEKVI DTFYVTDLFG QKVTNDNRQA 900
    SIATRLKAVM SEQEDELRDR MPNGIIAHPD VAALPATRTA KA 942
    Length:942
    Mass (Da):106,259
    Last modified:May 3, 2011 - v1
    Checksum:i9C1AC9C8AB73259B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002248 Genomic DNA. Translation: ADY63324.1.
    RefSeqiYP_004277644.1. NC_015183.1.

    Genome annotation databases

    EnsemblBacteriaiADY63324; ADY63324; AGROH133_03522.
    GeneIDi10266091.
    KEGGiagr:AGROH133_03522.
    PATRICi46845387. VBIAgrSp164909_0335.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002248 Genomic DNA. Translation: ADY63324.1 .
    RefSeqi YP_004277644.1. NC_015183.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADY63324 ; ADY63324 ; AGROH133_03522 .
    GeneIDi 10266091.
    KEGGi agr:AGROH133_03522.
    PATRICi 46845387. VBIAgrSp164909_0335.

    Phylogenomic databases

    KOi K00990.
    OMAi LYCLWDM.

    Enzyme and pathway databases

    BioCyci ASP861208:GH59-335-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequencing of Agrobacterium sp H13-3, the former Rhizobium lupini H13-3, reveals a tripartite genome consisting of a circular and a linear chromosome and an accessory plasmid but lacking a tumor-inducing Ti-plasmid."
      Wibberg D., Blom J., Jaenicke S., Kollin F., Rupp O., Scharf B., Schneiker-Bekel S., Sczcepanowski R., Goesmann A., Setubal J.C., Schmitt R., Puhler A., Schluter A.
      J. Biotechnol. 155:50-62(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: H13-3Imported.

    Entry informationi

    Entry nameiF0L5E2_AGRSH
    AccessioniPrimary (citable) accession number: F0L5E2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 3, 2011
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 28 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported, Multifunctional enzymeUniRule annotationSAAS annotation

    External Data

    Dasty 3