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F0L5E2

- F0L5E2_AGRSH

UniProt

F0L5E2 - F0L5E2_AGRSH

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, AGROH133_03522
Organism
Agrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3))
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotations
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotationSAAS annotations

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationSAAS annotations, NucleotidyltransferaseUniRule annotationSAAS annotationsImported, Transferase

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciASP861208:GH59-335-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Gene namesi
Name:glnDUniRule annotationImported
Ordered Locus Names:AGROH133_03522Imported
OrganismiAgrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3))Imported
Taxonomic identifieri861208 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium
ProteomesiUP000007455: Chromosome circular

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini523 – 60684HD By similarityUniRule annotation
Add
BLAST
Domaini730 – 81182ACT 1 By similarityUniRule annotation
Add
BLAST
Domaini841 – 92080ACT 2 By similarityUniRule annotation
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 373373Uridylyltransferase By similarityUniRule annotation
Add
BLAST
Regioni374 – 729356Uridylyl-removing By similarityUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 1 HD domain.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains ACT domains.SAAS annotations
Contains HD domain.SAAS annotations

Keywords - Domaini

RepeatUniRule annotationSAAS annotations

Phylogenomic databases

KOiK00990.
OMAiLYCLWDM.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F0L5E2-1 [UniParc]FASTAAdd to Basket

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MAIKDLDFSE ILDVSALKRE CDIIFKNDGK RIADVRSDLL PIFRKASTEG    50
REKARELLKS DGSGIDCARR ISWLQDRLIE ILYDLACQYV YPKDAPQIAV 100
AAVGGYGRGT LAPGSDIDLL FLLPAKNTPD MHKAVEFVLY LLWDLGFKVG 150
HATRTVDECV RLSKSDMTIR TAILEVRAIC GKKSLTDDLE KRFESEVVTG 200
TGPEFIAAKL AERDQRHRKA GDTRYLVEPN VKEGKGGLRD LHTLFWIAKY 250
YYHVRDTADL VKLGVLSKRE LRLFEKADDF LWAVRCQMHF ITNKAEERLS 300
FDIQREIADA LGYQPRPGLS AVERFMKHYF LVAKDVGDLT RIVCAALEDR 350
QAKDVPGLSG VLSRFAHRVR KIPGSVEFVE DRGRIALAKP DVFKNDPVNL 400
IRLFHIADIN NLELHPDALR VVTRSLSLIN DGLRENEEAN RLFLAILTSR 450
RDPALTLRRM NEAGVLGKFI PEFGKIVAMM QFNMYHHYTV DEHLIRSVGV 500
LSEVDKGTAV DAHPLANQLM PGVEEREALY VAVLLHDVAK GRQEDHSIAG 550
ARVARKLCQR FRLTGKQTEM VVWLIEQHLL MSMVAQTRDL HDRKTITDFA 600
DKVQSMERLK MLLILTVCDI RAVGPGVWNG WKGQLLRSLY YETELLLSGG 650
FSEVSRKERA HIARQALYDA LEDWGQKARR KYTKLHYEPY LLTVALEDQV 700
RHTRFIREAD KQEKALSTMV RTHSFHAITE ITVLAPDHPR LLSIITGACA 750
AAGANIADAQ IFTTSDGRAL DTILINREFP IDEDETRRGN NVGKLIEEVL 800
SGRQRLPEMI ATRTKSRRKK SAFTIPPSVI ISNGLSNKFT VIEVECLDRP 850
GLLADMTAVI ADLSLDIHSA RITTFGEKVI DTFYVTDLFG QKVTNDNRQA 900
SIATRLKAVM SEQEDELRDR MPNGIIAHPD VAALPATRTA KA 942
Length:942
Mass (Da):106,259
Last modified:May 3, 2011 - v1
Checksum:i9C1AC9C8AB73259B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002248 Genomic DNA. Translation: ADY63324.1.
RefSeqiYP_004277644.1. NC_015183.1.

Genome annotation databases

EnsemblBacteriaiADY63324; ADY63324; AGROH133_03522.
GeneIDi10266091.
KEGGiagr:AGROH133_03522.
PATRICi46845387. VBIAgrSp164909_0335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002248 Genomic DNA. Translation: ADY63324.1 .
RefSeqi YP_004277644.1. NC_015183.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADY63324 ; ADY63324 ; AGROH133_03522 .
GeneIDi 10266091.
KEGGi agr:AGROH133_03522.
PATRICi 46845387. VBIAgrSp164909_0335.

Phylogenomic databases

KOi K00990.
OMAi LYCLWDM.

Enzyme and pathway databases

BioCyci ASP861208:GH59-335-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequencing of Agrobacterium sp. H13-3, the former Rhizobium lupini H13-3, reveals a tripartite genome consisting of a circular and a linear chromosome and an accessory plasmid but lacking a tumor-inducing Ti-plasmid."
    Wibberg D., Blom J., Jaenicke S., Kollin F., Rupp O., Scharf B., Schneiker-Bekel S., Sczcepanowski R., Goesmann A., Setubal J.C., Schmitt R., Puhler A., Schluter A.
    J. Biotechnol. 155:50-62(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H13-3Imported.

Entry informationi

Entry nameiF0L5E2_AGRSH
AccessioniPrimary (citable) accession number: F0L5E2
Entry historyi
Integrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: May 14, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotations

External Data

Dasty 3

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