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Protein

Malate synthase G

Gene

glcB

Organism
Agrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3))
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotationSAAS annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei273Acetyl-CoAUniRule annotation1
Binding sitei310Acetyl-CoAUniRule annotation1
Active sitei337Proton acceptorUniRule annotation1
Binding sitei337GlyoxylateUniRule annotation1
Metal bindingi429MagnesiumUniRule annotation1
Binding sitei429GlyoxylateUniRule annotation1
Metal bindingi457MagnesiumUniRule annotation1
Binding sitei538Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei628Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotation
Biological processGlyoxylate bypassUniRule annotationSAAS annotation, Tricarboxylic acid cycleUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotationImported
Ordered Locus Names:AGROH133_02861Imported
OrganismiAgrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3))Imported
Taxonomic identifieri861208 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium
Proteomesi
  • UP000007455 Componenti: Chromosome circular

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei614Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

OxidationUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi861208.AGROH133_02861

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 124Acetyl-CoA bindingUniRule annotation2
Regioni454 – 457Glyoxylate bindingUniRule annotation4

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili49 – 69Sequence analysisAdd BLAST21
Coiled coili427 – 447Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4107QP3 Bacteria
COG2225 LUCA
KOiK01638
OMAiTEPVLHA
OrthoDBiPOG091H05P0

Family and domain databases

CDDicd00728 malate_synt_G, 1 hit
Gene3Di2.170.170.11, 1 hit
HAMAPiMF_00641 Malate_synth_G, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR023310 Malate_synth_G_beta_sub_dom
IPR001465 Malate_synthase
IPR006253 Malate_synthG
PANTHERiPTHR42739 PTHR42739, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01345 malate_syn_G, 1 hit

Sequencei

Sequence statusi: Complete.

F0L309-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRTNKFGLS IDDRLYAFLT DDVLPGTGFD AETFFEGFSA IVHELSPKNR
60 70 80 90 100
ELLEKRDALQ EKLDGWYRQN GAPTDFDVYE GFLKEIGYLL PEGPGFKVET
110 120 130 140 150
SNVDSEIAAV AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAISDAD
160 170 180 190 200
GAEKGRGYNP KRGEKVIAWA RNFLNESAPL ETAGWSDVTG FKIVDGLLQL
210 220 230 240 250
AIGDSKTGLK DAEQFKGFTG DAEKPATILL GKNGLHTEIV IDPSTEIGKG
260 270 280 290 300
DKAGISDVIL ESALTTIMDC EDSVAAVDAE DKVLVYGNWL GLMRGDLTEK
310 320 330 340 350
VSKGGNTFTR SLNPDRYYTA PDGGALTLPG RSLMLVRNVG HLMTNPAILD
360 370 380 390 400
KDGREVPEGI MDAVVTALIA LYDVGPSGRR QNSRAGSMYV VKPKMHGPEE
410 420 430 440 450
VAFANEIFTR AEKLVGMAPN TMKMGIMDEE RRTTVNLKES IRAAKDRVVF
460 470 480 490 500
INTGFLDRTG DEIHTSMEAG PMIRKGDMKQ AAWIAAYENW NVDIGLECGL
510 520 530 540 550
SGNAQIGKGM WAMPDLMAAM LEQKIAHPKA GANTAWVPSP TAATLHATHY
560 570 580 590 600
HKVDVAAVQD DLKSRGRAKL SDILSVPVVP RPNWTPEEIQ RELDNNAQGI
610 620 630 640 650
LGYVVRWVDQ GVGCSKVPDI NNVGLMEDRA TLRISAQHMA NWLRHGVVTQ
660 670 680 690 700
EQIVETMKRM AAVVDSQNAG DPAYLPMASN FEGSVAFQAA VELVLKGREQ
710 720
PNGYTEPVLH RRRLELKAKQ AA
Length:722
Mass (Da):78,710
Last modified:May 3, 2011 - v1
Checksum:i9786A22B45A57D54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002248 Genomic DNA Translation: ADY63045.1
RefSeqiWP_013635202.1, NC_015183.1

Genome annotation databases

EnsemblBacteriaiADY63045; ADY63045; AGROH133_02861
KEGGiagr:AGROH133_02861

Similar proteinsi

Entry informationi

Entry nameiF0L309_AGRSH
AccessioniPrimary (citable) accession number: F0L309
Entry historyiIntegrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: April 25, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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