SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

F0L309

- F0L309_AGRSH

UniProt

F0L309 - F0L309_AGRSH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Malate synthase G
Gene
glcB, AGROH133_02861
Organism
Agrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3))
Status
Unreviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.UniRule annotationSAAS annotations

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161Acetyl-CoA; via carbonyl oxygen By similarityUniRule annotation
Binding sitei273 – 2731Acetyl-CoA By similarityUniRule annotation
Binding sitei310 – 3101Acetyl-CoA By similarityUniRule annotation
Active sitei337 – 3371Proton acceptor By similarityUniRule annotation
Binding sitei337 – 3371Glyoxylate By similarityUniRule annotation
Metal bindingi429 – 4291Magnesium By similarityUniRule annotation
Binding sitei429 – 4291Glyoxylate By similarityUniRule annotation
Metal bindingi457 – 4571Magnesium By similarityUniRule annotation
Binding sitei538 – 5381Acetyl-CoA; via carbonyl oxygen By similarityUniRule annotation
Active sitei628 – 6281Proton donor By similarityUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationSAAS annotations

Keywords - Biological processi

Glyoxylate bypassUniRule annotationSAAS annotations, Tricarboxylic acid cycleUniRule annotationSAAS annotations

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciASP861208:GH59-48-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotationImported
Ordered Locus Names:AGROH133_02861Imported
OrganismiAgrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3))Imported
Taxonomic identifieri861208 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium
ProteomesiUP000007455: Chromosome circular

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei614 – 6141Cysteine sulfenic acid (-SOH) By similarityUniRule annotation

Keywords - PTMi

OxidationUniRule annotation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1242Acetyl-CoA binding By similarityUniRule annotation
Regioni454 – 4574Glyoxylate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

KOiK01638.
OMAiARTENVD.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

F0L309-1 [UniParc]FASTAAdd to Basket

« Hide

MSRTNKFGLS IDDRLYAFLT DDVLPGTGFD AETFFEGFSA IVHELSPKNR    50
ELLEKRDALQ EKLDGWYRQN GAPTDFDVYE GFLKEIGYLL PEGPGFKVET 100
SNVDSEIAAV AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAISDAD 150
GAEKGRGYNP KRGEKVIAWA RNFLNESAPL ETAGWSDVTG FKIVDGLLQL 200
AIGDSKTGLK DAEQFKGFTG DAEKPATILL GKNGLHTEIV IDPSTEIGKG 250
DKAGISDVIL ESALTTIMDC EDSVAAVDAE DKVLVYGNWL GLMRGDLTEK 300
VSKGGNTFTR SLNPDRYYTA PDGGALTLPG RSLMLVRNVG HLMTNPAILD 350
KDGREVPEGI MDAVVTALIA LYDVGPSGRR QNSRAGSMYV VKPKMHGPEE 400
VAFANEIFTR AEKLVGMAPN TMKMGIMDEE RRTTVNLKES IRAAKDRVVF 450
INTGFLDRTG DEIHTSMEAG PMIRKGDMKQ AAWIAAYENW NVDIGLECGL 500
SGNAQIGKGM WAMPDLMAAM LEQKIAHPKA GANTAWVPSP TAATLHATHY 550
HKVDVAAVQD DLKSRGRAKL SDILSVPVVP RPNWTPEEIQ RELDNNAQGI 600
LGYVVRWVDQ GVGCSKVPDI NNVGLMEDRA TLRISAQHMA NWLRHGVVTQ 650
EQIVETMKRM AAVVDSQNAG DPAYLPMASN FEGSVAFQAA VELVLKGREQ 700
PNGYTEPVLH RRRLELKAKQ AA 722
Length:722
Mass (Da):78,710
Last modified:May 3, 2011 - v1
Checksum:i9786A22B45A57D54
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002248 Genomic DNA. Translation: ADY63045.1.
RefSeqiWP_013635202.1. NC_015183.1.
YP_004277365.1. NC_015183.1.

Genome annotation databases

EnsemblBacteriaiADY63045; ADY63045; AGROH133_02861.
GeneIDi10265805.
KEGGiagr:AGROH133_02861.
PATRICi46844791. VBIAgrSp164909_0047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002248 Genomic DNA. Translation: ADY63045.1 .
RefSeqi WP_013635202.1. NC_015183.1.
YP_004277365.1. NC_015183.1.

3D structure databases

ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADY63045 ; ADY63045 ; AGROH133_02861 .
GeneIDi 10265805.
KEGGi agr:AGROH133_02861.
PATRICi 46844791. VBIAgrSp164909_0047.

Phylogenomic databases

KOi K01638.
OMAi ARTENVD.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci ASP861208:GH59-48-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequencing of Agrobacterium sp. H13-3, the former Rhizobium lupini H13-3, reveals a tripartite genome consisting of a circular and a linear chromosome and an accessory plasmid but lacking a tumor-inducing Ti-plasmid."
    Wibberg D., Blom J., Jaenicke S., Kollin F., Rupp O., Scharf B., Schneiker-Bekel S., Sczcepanowski R., Goesmann A., Setubal J.C., Schmitt R., Puhler A., Schluter A.
    J. Biotechnol. 155:50-62(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H13-3Imported.

Entry informationi

Entry nameiF0L309_AGRSH
AccessioniPrimary (citable) accession number: F0L309
Entry historyi
Integrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: September 3, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

Similar proteinsi