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F0L309

- F0L309_AGRSH

UniProt

F0L309 - F0L309_AGRSH

Protein

Malate synthase G

Gene

glcB

Organism
Agrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3))
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 28 (01 Oct 2014)
      Sequence version 1 (03 May 2011)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotationSAAS annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei273 – 2731Acetyl-CoAUniRule annotation
    Binding sitei310 – 3101Acetyl-CoAUniRule annotation
    Active sitei337 – 3371Proton acceptorUniRule annotation
    Binding sitei337 – 3371GlyoxylateUniRule annotation
    Metal bindingi429 – 4291MagnesiumUniRule annotation
    Binding sitei429 – 4291GlyoxylateUniRule annotation
    Metal bindingi457 – 4571MagnesiumUniRule annotation
    Binding sitei538 – 5381Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei628 – 6281Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    TransferaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    Glyoxylate bypassUniRule annotationSAAS annotation, Tricarboxylic acid cycleUniRule annotationSAAS annotation

    Keywords - Ligandi

    MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciASP861208:GH59-48-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotationImported
    Ordered Locus Names:AGROH133_02861Imported
    OrganismiAgrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3))Imported
    Taxonomic identifieri861208 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium
    ProteomesiUP000007455: Chromosome circular

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei614 – 6141Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    OxidationUniRule annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni123 – 1242Acetyl-CoA bindingUniRule annotation
    Regioni454 – 4574Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    KOiK01638.
    OMAiARTENVD.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    F0L309-1 [UniParc]FASTAAdd to Basket

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    MSRTNKFGLS IDDRLYAFLT DDVLPGTGFD AETFFEGFSA IVHELSPKNR    50
    ELLEKRDALQ EKLDGWYRQN GAPTDFDVYE GFLKEIGYLL PEGPGFKVET 100
    SNVDSEIAAV AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAISDAD 150
    GAEKGRGYNP KRGEKVIAWA RNFLNESAPL ETAGWSDVTG FKIVDGLLQL 200
    AIGDSKTGLK DAEQFKGFTG DAEKPATILL GKNGLHTEIV IDPSTEIGKG 250
    DKAGISDVIL ESALTTIMDC EDSVAAVDAE DKVLVYGNWL GLMRGDLTEK 300
    VSKGGNTFTR SLNPDRYYTA PDGGALTLPG RSLMLVRNVG HLMTNPAILD 350
    KDGREVPEGI MDAVVTALIA LYDVGPSGRR QNSRAGSMYV VKPKMHGPEE 400
    VAFANEIFTR AEKLVGMAPN TMKMGIMDEE RRTTVNLKES IRAAKDRVVF 450
    INTGFLDRTG DEIHTSMEAG PMIRKGDMKQ AAWIAAYENW NVDIGLECGL 500
    SGNAQIGKGM WAMPDLMAAM LEQKIAHPKA GANTAWVPSP TAATLHATHY 550
    HKVDVAAVQD DLKSRGRAKL SDILSVPVVP RPNWTPEEIQ RELDNNAQGI 600
    LGYVVRWVDQ GVGCSKVPDI NNVGLMEDRA TLRISAQHMA NWLRHGVVTQ 650
    EQIVETMKRM AAVVDSQNAG DPAYLPMASN FEGSVAFQAA VELVLKGREQ 700
    PNGYTEPVLH RRRLELKAKQ AA 722
    Length:722
    Mass (Da):78,710
    Last modified:May 3, 2011 - v1
    Checksum:i9786A22B45A57D54
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002248 Genomic DNA. Translation: ADY63045.1.
    RefSeqiWP_013635202.1. NC_015183.1.
    YP_004277365.1. NC_015183.1.

    Genome annotation databases

    EnsemblBacteriaiADY63045; ADY63045; AGROH133_02861.
    GeneIDi10265805.
    KEGGiagr:AGROH133_02861.
    PATRICi46844791. VBIAgrSp164909_0047.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002248 Genomic DNA. Translation: ADY63045.1 .
    RefSeqi WP_013635202.1. NC_015183.1.
    YP_004277365.1. NC_015183.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADY63045 ; ADY63045 ; AGROH133_02861 .
    GeneIDi 10265805.
    KEGGi agr:AGROH133_02861.
    PATRICi 46844791. VBIAgrSp164909_0047.

    Phylogenomic databases

    KOi K01638.
    OMAi ARTENVD.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci ASP861208:GH59-48-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequencing of Agrobacterium sp H13-3, the former Rhizobium lupini H13-3, reveals a tripartite genome consisting of a circular and a linear chromosome and an accessory plasmid but lacking a tumor-inducing Ti-plasmid."
      Wibberg D., Blom J., Jaenicke S., Kollin F., Rupp O., Scharf B., Schneiker-Bekel S., Sczcepanowski R., Goesmann A., Setubal J.C., Schmitt R., Puhler A., Schluter A.
      J. Biotechnol. 155:50-62(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: H13-3Imported.

    Entry informationi

    Entry nameiF0L309_AGRSH
    AccessioniPrimary (citable) accession number: F0L309
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 3, 2011
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 28 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3