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F0L309

- F0L309_AGRSH

UniProt

F0L309 - F0L309_AGRSH

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Protein

Malate synthase G

Gene

glcB

Organism
Agrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3))
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotationSAAS annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotationSAAS annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei273 – 2731Acetyl-CoAUniRule annotation
Binding sitei310 – 3101Acetyl-CoAUniRule annotation
Active sitei337 – 3371Proton acceptorUniRule annotation
Binding sitei337 – 3371GlyoxylateUniRule annotation
Metal bindingi429 – 4291MagnesiumUniRule annotation
Binding sitei429 – 4291GlyoxylateUniRule annotation
Metal bindingi457 – 4571MagnesiumUniRule annotation
Binding sitei538 – 5381Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei628 – 6281Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotation

Keywords - Biological processi

Glyoxylate bypassUniRule annotation, Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciASP861208:GH59-48-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotationImported
Ordered Locus Names:AGROH133_02861Imported
OrganismiAgrobacterium sp. (strain H13-3) (Rhizobium lupini (strain H13-3))Imported
Taxonomic identifieri861208 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium
ProteomesiUP000007455: Chromosome circular

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei614 – 6141Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

OxidationUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1242Acetyl-CoA bindingUniRule annotation
Regioni454 – 4574Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

KOiK01638.
OMAiARTENVD.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

F0L309 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRTNKFGLS IDDRLYAFLT DDVLPGTGFD AETFFEGFSA IVHELSPKNR
60 70 80 90 100
ELLEKRDALQ EKLDGWYRQN GAPTDFDVYE GFLKEIGYLL PEGPGFKVET
110 120 130 140 150
SNVDSEIAAV AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAISDAD
160 170 180 190 200
GAEKGRGYNP KRGEKVIAWA RNFLNESAPL ETAGWSDVTG FKIVDGLLQL
210 220 230 240 250
AIGDSKTGLK DAEQFKGFTG DAEKPATILL GKNGLHTEIV IDPSTEIGKG
260 270 280 290 300
DKAGISDVIL ESALTTIMDC EDSVAAVDAE DKVLVYGNWL GLMRGDLTEK
310 320 330 340 350
VSKGGNTFTR SLNPDRYYTA PDGGALTLPG RSLMLVRNVG HLMTNPAILD
360 370 380 390 400
KDGREVPEGI MDAVVTALIA LYDVGPSGRR QNSRAGSMYV VKPKMHGPEE
410 420 430 440 450
VAFANEIFTR AEKLVGMAPN TMKMGIMDEE RRTTVNLKES IRAAKDRVVF
460 470 480 490 500
INTGFLDRTG DEIHTSMEAG PMIRKGDMKQ AAWIAAYENW NVDIGLECGL
510 520 530 540 550
SGNAQIGKGM WAMPDLMAAM LEQKIAHPKA GANTAWVPSP TAATLHATHY
560 570 580 590 600
HKVDVAAVQD DLKSRGRAKL SDILSVPVVP RPNWTPEEIQ RELDNNAQGI
610 620 630 640 650
LGYVVRWVDQ GVGCSKVPDI NNVGLMEDRA TLRISAQHMA NWLRHGVVTQ
660 670 680 690 700
EQIVETMKRM AAVVDSQNAG DPAYLPMASN FEGSVAFQAA VELVLKGREQ
710 720
PNGYTEPVLH RRRLELKAKQ AA
Length:722
Mass (Da):78,710
Last modified:May 3, 2011 - v1
Checksum:i9786A22B45A57D54
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002248 Genomic DNA. Translation: ADY63045.1.
RefSeqiWP_013635202.1. NC_015183.1.
YP_004277365.1. NC_015183.1.

Genome annotation databases

EnsemblBacteriaiADY63045; ADY63045; AGROH133_02861.
GeneIDi10265805.
KEGGiagr:AGROH133_02861.
PATRICi46844791. VBIAgrSp164909_0047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002248 Genomic DNA. Translation: ADY63045.1 .
RefSeqi WP_013635202.1. NC_015183.1.
YP_004277365.1. NC_015183.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADY63045 ; ADY63045 ; AGROH133_02861 .
GeneIDi 10265805.
KEGGi agr:AGROH133_02861.
PATRICi 46844791. VBIAgrSp164909_0047.

Phylogenomic databases

KOi K01638.
OMAi ARTENVD.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci ASP861208:GH59-48-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequencing of Agrobacterium sp H13-3, the former Rhizobium lupini H13-3, reveals a tripartite genome consisting of a circular and a linear chromosome and an accessory plasmid but lacking a tumor-inducing Ti-plasmid."
    Wibberg D., Blom J., Jaenicke S., Kollin F., Rupp O., Scharf B., Schneiker-Bekel S., Sczcepanowski R., Goesmann A., Setubal J.C., Schmitt R., Puhler A., Schluter A.
    J. Biotechnol. 155:50-62(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H13-3Imported.

Entry informationi

Entry nameiF0L309_AGRSH
AccessioniPrimary (citable) accession number: F0L309
Entry historyi
Integrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: October 29, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3