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Protein

Siroheme synthase

Gene

cysG

Organism
Acinetobacter calcoaceticus (strain PHEA-2)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotationSAAS annotation

Catalytic activityi

Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotationSAAS annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotationSAAS annotation
S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotationSAAS annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotationSAAS annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes siroheme from sirohydrochlorin, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei248Proton acceptorUniRule annotation1
Active sitei270Proton donorUniRule annotation1
Binding sitei306S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei383S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei412S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 23NADUniRule annotation2
Nucleotide bindingi43 – 44NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationSAAS annotation, MethyltransferaseUniRule annotationSAAS annotation, OxidoreductaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

Cobalamin biosynthesisUniRule annotationSAAS annotation, Porphyrin biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

NADUniRule annotationSAAS annotation, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthaseUniRule annotation
Gene namesi
Name:cysGUniRule annotationImported
Ordered Locus Names:BDGL_002175Imported
OrganismiAcinetobacter calcoaceticus (strain PHEA-2)Imported
Taxonomic identifieri871585 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex
Proteomesi
  • UP000007477 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei129PhosphoserineUniRule annotation1

Keywords - PTMi

PhosphoproteinUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi871585.BDGL_002175.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 116NAD_binding_7InterPro annotationAdd BLAST110
Domaini152 – 208CysG_dimeriserInterPro annotationAdd BLAST57
Domaini218 – 427TP_methylaseInterPro annotationAdd BLAST210

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 204precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd BLAST204
Regioni216 – 457Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd BLAST242
Regioni301 – 303S-adenosyl-L-methionine bindingUniRule annotation3
Regioni331 – 332S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CB8. Bacteria.
COG0007. LUCA.
COG1648. LUCA.
KOiK02302.
OMAiQASFIMP.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F0KMG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIFPISLKL QQQYCLIVGG GHIALRKANL LAKAGAVIDI IAPAIEEQLL
60 70 80 90 100
QLVKTTGGEY FAESFAEKIL NTPYRLVIAA TNDAQVNKAV FEQCEARNLL
110 120 130 140 150
VNSVDDIPHC RFMVPAIIDR SPLIISVASN GASPVLSRQL RTQIETIVPH
160 170 180 190 200
GMGKLAEFSG QWRKQVKEKI PNPDERRIFW ENLYASPLKE QVFNDNLEVA
210 220 230 240 250
NGLIQQALTE WTAPKGEVYL VGAGPGDPEL LTLKALRLMQ QADVVIYDRL
260 270 280 290 300
VSAPILELCR RDATKIYVGK ARSNHSVPQD GINALLVEYA QKGKRVCRLK
310 320 330 340 350
GGDPFIFGRG GEEIQELVEA NVTFQVVPGI TAASGCSAYA GIPLTHRDYA
360 370 380 390 400
QSVRFLTGHL KEGSPELPWN ELVYENQTLV LYMGLVGLER ICEQLIAHGQ
410 420 430 440 450
RADMPVALIS KGTTPDQKVV VGTLADIATK VSEHHIVAPT LTIIGEVVNL

REQLKWQ
Length:457
Mass (Da):50,291
Last modified:May 3, 2011 - v1
Checksum:iBA82A6BACB08FC81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002177 Genomic DNA. Translation: ADY82761.1.
RefSeqiWP_014207488.1. NC_016603.1.
YP_004996443.1. NC_016603.1.

Genome annotation databases

EnsemblBacteriaiADY82761; ADY82761; BDGL_002175.
GeneIDi11636698.
KEGGiacc:BDGL_002175.
PATRICi47082490. VBIAciCal168233_2176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002177 Genomic DNA. Translation: ADY82761.1.
RefSeqiWP_014207488.1. NC_016603.1.
YP_004996443.1. NC_016603.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi871585.BDGL_002175.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADY82761; ADY82761; BDGL_002175.
GeneIDi11636698.
KEGGiacc:BDGL_002175.
PATRICi47082490. VBIAciCal168233_2176.

Phylogenomic databases

eggNOGiENOG4105CB8. Bacteria.
COG0007. LUCA.
COG1648. LUCA.
KOiK02302.
OMAiQASFIMP.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiF0KMG6_ACICP
AccessioniPrimary (citable) accession number: F0KMG6
Entry historyi
Integrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: November 2, 2016
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported, Multifunctional enzymeUniRule annotationSAAS annotation

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.