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F0KMG6

- F0KMG6_ACICP

UniProt

F0KMG6 - F0KMG6_ACICP

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Protein
Siroheme synthase
Gene
cysG, BDGL_002175
Organism
Acinetobacter calcoaceticus (strain PHEA-2)
Status
Unreviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity.UniRule annotationSAAS annotations

Catalytic activityi

Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotationSAAS annotations
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotationSAAS annotations
S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotationSAAS annotations
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotationSAAS annotations

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei225 – 2251S-adenosyl-L-methionine; via carbonyl oxygen By similarityUniRule annotation
Active sitei248 – 2481Proton acceptor By similarityUniRule annotation
Active sitei270 – 2701Proton donor By similarityUniRule annotation
Binding sitei306 – 3061S-adenosyl-L-methionine; via carbonyl oxygen By similarityUniRule annotation
Binding sitei383 – 3831S-adenosyl-L-methionine; via amide nitrogen By similarityUniRule annotation
Binding sitei412 – 4121S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NAD By similarityUniRule annotation
Nucleotide bindingi43 – 442NAD By similarityUniRule annotation

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: InterPro
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationSAAS annotations, MethyltransferaseUniRule annotationSAAS annotations, OxidoreductaseUniRule annotationSAAS annotations, Transferase

Keywords - Biological processi

Cobalamin biosynthesisUniRule annotationSAAS annotations, Porphyrin biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

NADUniRule annotationSAAS annotations, S-adenosyl-L-methionineUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciACAL871585:GH86-2203-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthaseUniRule annotation
Gene namesi
Name:cysGUniRule annotationImported
Ordered Locus Names:BDGL_002175Imported
OrganismiAcinetobacter calcoaceticus (strain PHEA-2)Imported
Taxonomic identifieri871585 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex
ProteomesiUP000007477: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei129 – 1291Phosphoserine By similarityUniRule annotation

Keywords - PTMi

PhosphoproteinUniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 204204precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarityUniRule annotation
Add
BLAST
Regioni216 – 457242Uroporphyrinogen-III C-methyltransferase By similarityUniRule annotation
Add
BLAST
Regioni301 – 3033S-adenosyl-L-methionine binding By similarityUniRule annotation
Regioni331 – 3322S-adenosyl-L-methionine binding By similarityUniRule annotation

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation
In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation

Phylogenomic databases

KOiK02302.
OMAiQASFIMP.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F0KMG6-1 [UniParc]FASTAAdd to Basket

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MDIFPISLKL QQQYCLIVGG GHIALRKANL LAKAGAVIDI IAPAIEEQLL    50
QLVKTTGGEY FAESFAEKIL NTPYRLVIAA TNDAQVNKAV FEQCEARNLL 100
VNSVDDIPHC RFMVPAIIDR SPLIISVASN GASPVLSRQL RTQIETIVPH 150
GMGKLAEFSG QWRKQVKEKI PNPDERRIFW ENLYASPLKE QVFNDNLEVA 200
NGLIQQALTE WTAPKGEVYL VGAGPGDPEL LTLKALRLMQ QADVVIYDRL 250
VSAPILELCR RDATKIYVGK ARSNHSVPQD GINALLVEYA QKGKRVCRLK 300
GGDPFIFGRG GEEIQELVEA NVTFQVVPGI TAASGCSAYA GIPLTHRDYA 350
QSVRFLTGHL KEGSPELPWN ELVYENQTLV LYMGLVGLER ICEQLIAHGQ 400
RADMPVALIS KGTTPDQKVV VGTLADIATK VSEHHIVAPT LTIIGEVVNL 450
REQLKWQ 457
Length:457
Mass (Da):50,291
Last modified:May 3, 2011 - v1
Checksum:iBA82A6BACB08FC81
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002177 Genomic DNA. Translation: ADY82761.1.
RefSeqiWP_014207488.1. NC_016603.1.
YP_004996443.1. NC_016603.1.

Genome annotation databases

EnsemblBacteriaiADY82761; ADY82761; BDGL_002175.
GeneIDi11636698.
KEGGiacc:BDGL_002175.
PATRICi47082490. VBIAciCal168233_2176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002177 Genomic DNA. Translation: ADY82761.1 .
RefSeqi WP_014207488.1. NC_016603.1.
YP_004996443.1. NC_016603.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADY82761 ; ADY82761 ; BDGL_002175 .
GeneIDi 11636698.
KEGGi acc:BDGL_002175.
PATRICi 47082490. VBIAciCal168233_2176.

Phylogenomic databases

KOi K02302.
OMAi QASFIMP.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci ACAL871585:GH86-2203-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of a nonpathogenic wastewater-adapted bacterium Acinetobacter calcoaceticus PHEA-2 and comparative genomics insights into environmental adaptation."
    Zhan Y., Yan Y., Zhang W., Chen M., Ping S., Lu W., Lin M.
    Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: PHEA-2.
  2. "Genome sequence of Acinetobacter calcoaceticus PHEA-2, isolated from industry wastewater."
    Zhan Y., Yan Y., Zhang W., Yu H., Chen M., Lu W., Ping S., Peng Z., Yuan M., Zhou Z., Elmerich C., Lin M.
    J. Bacteriol. 193:2672-2673(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PHEA-2Imported.

Entry informationi

Entry nameiF0KMG6_ACICP
AccessioniPrimary (citable) accession number: F0KMG6
Entry historyi
Integrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: September 3, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotations

External Data

Dasty 3

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