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F0KMG6

- F0KMG6_ACICP

UniProt

F0KMG6 - F0KMG6_ACICP

Protein

Siroheme synthase

Gene

cysG

Organism
Acinetobacter calcoaceticus (strain PHEA-2)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 23 (01 Oct 2014)
      Sequence version 1 (03 May 2011)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotationSAAS annotation

    Catalytic activityi

    Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotationSAAS annotation
    S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotationSAAS annotation
    S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotationSAAS annotation
    Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei225 – 2251S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei248 – 2481Proton acceptorUniRule annotation
    Active sitei270 – 2701Proton donorUniRule annotation
    Binding sitei306 – 3061S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei383 – 3831S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
    Binding sitei412 – 4121S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi22 – 232NADUniRule annotation
    Nucleotide bindingi43 – 442NADUniRule annotation

    GO - Molecular functioni

    1. NAD binding Source: InterPro
    2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
    3. sirohydrochlorin ferrochelatase activity Source: InterPro
    4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
    2. siroheme biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    LyaseUniRule annotationSAAS annotation, MethyltransferaseUniRule annotationSAAS annotation, OxidoreductaseUniRule annotationSAAS annotation, Transferase

    Keywords - Biological processi

    Cobalamin biosynthesisUniRule annotationSAAS annotation, Porphyrin biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    NADUniRule annotationSAAS annotation, S-adenosyl-L-methionineUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciACAL871585:GH86-2203-MONOMER.
    UniPathwayiUPA00148; UER00211.
    UPA00148; UER00222.
    UPA00262; UER00211.
    UPA00262; UER00222.
    UPA00262; UER00376.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Siroheme synthaseUniRule annotation
    Gene namesi
    Name:cysGUniRule annotationImported
    Ordered Locus Names:BDGL_002175Imported
    OrganismiAcinetobacter calcoaceticus (strain PHEA-2)Imported
    Taxonomic identifieri871585 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex
    ProteomesiUP000007477: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei129 – 1291PhosphoserineUniRule annotation

    Keywords - PTMi

    PhosphoproteinUniRule annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 204204precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd
    BLAST
    Regioni216 – 457242Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd
    BLAST
    Regioni301 – 3033S-adenosyl-L-methionine bindingUniRule annotation
    Regioni331 – 3322S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the precorrin methyltransferase family.UniRule annotation
    In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation
    In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation

    Phylogenomic databases

    KOiK02302.
    OMAiQASFIMP.

    Family and domain databases

    Gene3Di1.10.8.210. 1 hit.
    3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPiMF_01646. Siroheme_synth.
    InterProiIPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR016040. NAD(P)-bd_dom.
    IPR012409. Sirohaem_synth.
    IPR019478. Sirohaem_synthase_dimer_dom.
    IPR006367. Sirohaem_synthase_N.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view]
    PfamiPF10414. CysG_dimeriser. 1 hit.
    PF13241. NAD_binding_7. 1 hit.
    PF00590. TP_methylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
    SUPFAMiSSF53790. SSF53790. 1 hit.
    TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
    TIGR01470. cysG_Nterm. 1 hit.
    PROSITEiPS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    F0KMG6-1 [UniParc]FASTAAdd to Basket

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    MDIFPISLKL QQQYCLIVGG GHIALRKANL LAKAGAVIDI IAPAIEEQLL    50
    QLVKTTGGEY FAESFAEKIL NTPYRLVIAA TNDAQVNKAV FEQCEARNLL 100
    VNSVDDIPHC RFMVPAIIDR SPLIISVASN GASPVLSRQL RTQIETIVPH 150
    GMGKLAEFSG QWRKQVKEKI PNPDERRIFW ENLYASPLKE QVFNDNLEVA 200
    NGLIQQALTE WTAPKGEVYL VGAGPGDPEL LTLKALRLMQ QADVVIYDRL 250
    VSAPILELCR RDATKIYVGK ARSNHSVPQD GINALLVEYA QKGKRVCRLK 300
    GGDPFIFGRG GEEIQELVEA NVTFQVVPGI TAASGCSAYA GIPLTHRDYA 350
    QSVRFLTGHL KEGSPELPWN ELVYENQTLV LYMGLVGLER ICEQLIAHGQ 400
    RADMPVALIS KGTTPDQKVV VGTLADIATK VSEHHIVAPT LTIIGEVVNL 450
    REQLKWQ 457
    Length:457
    Mass (Da):50,291
    Last modified:May 3, 2011 - v1
    Checksum:iBA82A6BACB08FC81
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002177 Genomic DNA. Translation: ADY82761.1.
    RefSeqiWP_014207488.1. NC_016603.1.
    YP_004996443.1. NC_016603.1.

    Genome annotation databases

    EnsemblBacteriaiADY82761; ADY82761; BDGL_002175.
    GeneIDi11636698.
    KEGGiacc:BDGL_002175.
    PATRICi47082490. VBIAciCal168233_2176.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002177 Genomic DNA. Translation: ADY82761.1 .
    RefSeqi WP_014207488.1. NC_016603.1.
    YP_004996443.1. NC_016603.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADY82761 ; ADY82761 ; BDGL_002175 .
    GeneIDi 11636698.
    KEGGi acc:BDGL_002175.
    PATRICi 47082490. VBIAciCal168233_2176.

    Phylogenomic databases

    KOi K02302.
    OMAi QASFIMP.

    Enzyme and pathway databases

    UniPathwayi UPA00148 ; UER00211 .
    UPA00148 ; UER00222 .
    UPA00262 ; UER00211 .
    UPA00262 ; UER00222 .
    UPA00262 ; UER00376 .
    BioCyci ACAL871585:GH86-2203-MONOMER.

    Family and domain databases

    Gene3Di 1.10.8.210. 1 hit.
    3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPi MF_01646. Siroheme_synth.
    InterProi IPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR016040. NAD(P)-bd_dom.
    IPR012409. Sirohaem_synth.
    IPR019478. Sirohaem_synthase_dimer_dom.
    IPR006367. Sirohaem_synthase_N.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view ]
    Pfami PF10414. CysG_dimeriser. 1 hit.
    PF13241. NAD_binding_7. 1 hit.
    PF00590. TP_methylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
    SUPFAMi SSF53790. SSF53790. 1 hit.
    TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
    TIGR01470. cysG_Nterm. 1 hit.
    PROSITEi PS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of a nonpathogenic wastewater-adapted bacterium Acinetobacter calcoaceticus PHEA-2 and comparative genomics insights into environmental adaptation."
      Zhan Y., Yan Y., Zhang W., Chen M., Ping S., Lu W., Lin M.
      Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: PHEA-2.
    2. "Genome sequence of Acinetobacter calcoaceticus PHEA-2, isolated from industry wastewater."
      Zhan Y., Yan Y., Zhang W., Yu H., Chen M., Lu W., Ping S., Peng Z., Yuan M., Zhou Z., Elmerich C., Lin M.
      J. Bacteriol. 193:2672-2673(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PHEA-2Imported.

    Entry informationi

    Entry nameiF0KMG6_ACICP
    AccessioniPrimary (citable) accession number: F0KMG6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 3, 2011
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 23 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported, Multifunctional enzymeUniRule annotationSAAS annotation

    External Data

    Dasty 3