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Protein

Siroheme synthase

Gene

cysG

Organism
Acinetobacter calcoaceticus (strain PHEA-2)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotationSAAS annotation

Catalytic activityi

2 S-adenosyl-L-methionine + uroporphyrinogen III = 2 S-adenosyl-L-homocysteine + precorrin-2.UniRule annotationSAAS annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotationSAAS annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotationSAAS annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes siroheme from sirohydrochlorin, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei248Proton acceptorUniRule annotation1
Active sitei270Proton donorUniRule annotation1
Binding sitei306S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei383S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei412S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 23NADUniRule annotation2
Nucleotide bindingi43 – 44NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation, MethyltransferaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, OxidoreductaseUniRule annotationSAAS annotation, Transferase
Biological processCobalamin biosynthesisUniRule annotationSAAS annotation, Porphyrin biosynthesisUniRule annotationSAAS annotation
LigandNADUniRule annotationSAAS annotation, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciACAL871585:G1GRU-2203-MONOMER
UniPathwayiUPA00148; UER00222
UPA00262; UER00211
UPA00262; UER00222
UPA00262; UER00376

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthaseUniRule annotation
Including the following 3 domains:
Precorrin-2 dehydrogenaseUniRule annotation (EC:1.3.1.76UniRule annotation)
Sirohydrochlorin ferrochelataseUniRule annotation (EC:4.99.1.4UniRule annotation)
Uroporphyrinogen-III C-methyltransferaseUniRule annotation (EC:2.1.1.107UniRule annotation)
Short name:
Urogen III methylaseUniRule annotation
Alternative name(s):
SUMTUniRule annotation
Uroporphyrinogen III methylaseUniRule annotation
Short name:
UROMUniRule annotation
Gene namesi
Name:cysGUniRule annotationImported
Ordered Locus Names:BDGL_002175Imported
OrganismiAcinetobacter calcoaceticus (strain PHEA-2)Imported
Taxonomic identifieri871585 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex
Proteomesi
  • UP000007477 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei129PhosphoserineUniRule annotation1

Keywords - PTMi

PhosphoproteinUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi871585.BDGL_002175

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini152 – 208CysG_dimeriserInterPro annotationAdd BLAST57
Domaini218 – 427TP_methylaseInterPro annotationAdd BLAST210

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 204precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd BLAST204
Regioni216 – 457Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd BLAST242
Regioni301 – 303S-adenosyl-L-methionine bindingUniRule annotation3
Regioni331 – 332S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CB8 Bacteria
COG0007 LUCA
COG1648 LUCA
KOiK02302
OMAiFCVRADD

Family and domain databases

CDDicd11642 SUMT, 1 hit
Gene3Di1.10.8.210, 1 hit
3.30.950.10, 1 hit
3.40.1010.10, 1 hit
HAMAPiMF_01646 Siroheme_synth, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR014776 4pyrrole_Mease_sub2
IPR006366 CobA/CysG_C
IPR036291 NAD(P)-bd_dom_sf
IPR037115 Sirohaem_synt_dimer_dom_sf
IPR012409 Sirohaem_synth
IPR019478 Sirohaem_synthase_dimer_dom
IPR006367 Sirohaem_synthase_N
IPR003043 Uropor_MeTrfase_CS
PfamiView protein in Pfam
PF10414 CysG_dimeriser, 1 hit
PF00590 TP_methylase, 1 hit
PIRSFiPIRSF036426 Sirohaem_synth, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR01469 cobA_cysG_Cterm, 1 hit
TIGR01470 cysG_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS00839 SUMT_1, 1 hit
PS00840 SUMT_2, 1 hit

Sequencei

Sequence statusi: Complete.

F0KMG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIFPISLKL QQQYCLIVGG GHIALRKANL LAKAGAVIDI IAPAIEEQLL
60 70 80 90 100
QLVKTTGGEY FAESFAEKIL NTPYRLVIAA TNDAQVNKAV FEQCEARNLL
110 120 130 140 150
VNSVDDIPHC RFMVPAIIDR SPLIISVASN GASPVLSRQL RTQIETIVPH
160 170 180 190 200
GMGKLAEFSG QWRKQVKEKI PNPDERRIFW ENLYASPLKE QVFNDNLEVA
210 220 230 240 250
NGLIQQALTE WTAPKGEVYL VGAGPGDPEL LTLKALRLMQ QADVVIYDRL
260 270 280 290 300
VSAPILELCR RDATKIYVGK ARSNHSVPQD GINALLVEYA QKGKRVCRLK
310 320 330 340 350
GGDPFIFGRG GEEIQELVEA NVTFQVVPGI TAASGCSAYA GIPLTHRDYA
360 370 380 390 400
QSVRFLTGHL KEGSPELPWN ELVYENQTLV LYMGLVGLER ICEQLIAHGQ
410 420 430 440 450
RADMPVALIS KGTTPDQKVV VGTLADIATK VSEHHIVAPT LTIIGEVVNL

REQLKWQ
Length:457
Mass (Da):50,291
Last modified:May 3, 2011 - v1
Checksum:iBA82A6BACB08FC81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002177 Genomic DNA Translation: ADY82761.1
RefSeqiWP_014207488.1, NC_016603.1
YP_004996443.1, NC_016603.1

Genome annotation databases

EnsemblBacteriaiADY82761; ADY82761; BDGL_002175
GeneIDi11636698
KEGGiacc:BDGL_002175
PATRICifig|871585.3.peg.2176

Similar proteinsi

Entry informationi

Entry nameiF0KMG6_ACICP
AccessioniPrimary (citable) accession number: F0KMG6
Entry historyiIntegrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: June 20, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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