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F0KCN9 (F0KCN9_CLOAE) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Elongation factor 4 HAMAP-Rule MF_00071
Short name=EF-4 HAMAP-Rule MF_00071
EC=3.6.5.n1 HAMAP-Rule MF_00071
Alternative name(s):
Ribosomal back-translocase LepA HAMAP-Rule MF_00071
Gene names
Name:lepA HAMAP-Rule MF_00071 EMBL ADZ20328.1
Ordered Locus Names:CEA_G1291 EMBL ADZ20328.1
OrganismClostridium acetobutylicum (strain EA 2018) [Complete proteome] [HAMAP]
Taxonomic identifier863638 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium ›

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071

Catalytic activity

GTP + H2O = GDP + phosphate. HAMAP-Rule MF_00071

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.

Sequence similarities

Belongs to the GTP-binding elongation factor family. LepA subfamily. HAMAP-Rule MF_00071

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding19 – 246GTP By similarity HAMAP-Rule MF_00071
Nucleotide binding137 – 1404GTP By similarity HAMAP-Rule MF_00071

Sequences

Sequence LengthMass (Da)Tools
F0KCN9 [UniParc].

Last modified May 3, 2011. Version 1.
Checksum: 59248A058499BDF9

FASTA60267,612
        10         20         30         40         50         60 
MQSERQKHIR NFCIVAHIDH GKSTLADRLL EHTGTLTHRE MEEQVLDNME IERERGITIK 

        70         80         90        100        110        120 
SQAARLIYKR PEDGQEYILN LIDTPGHVDF NYEVSRSLAA CEGAVLVVDA TQGIQAQTLA 

       130        140        150        160        170        180 
NCYMAVDHGL EVVPVINKID LPSARPDEIK NEIEDVIGIE SSDAPLISAK TGLNIEDVLE 

       190        200        210        220        230        240 
AVVQKVPAPS GDEKGPLKAL IFDSYYDSYK GVVCHIRIKD GTVKAGTRIK LMAANKEYEV 

       250        260        270        280        290        300 
IEVGVFVPNY LKVEELKAGD VGYITASIKN VRDARVGDTI TEVNNPAKEP LLGYRPAISM 

       310        320        330        340        350        360 
VYSGIYPIDS SKYEELKEAL EKLQVNDAAL NFEPETSIAL GFGFRCGFLG LLHMEIMQER 

       370        380        390        400        410        420 
IEKEFNLDVI MTAPSVIYKV KKTDGTLIEI TNPTNLPEPT EIDYMEEPVV KAQIITPSDF 

       430        440        450        460        470        480 
VGAVMELCQN KRGTFIDMEY IETTRVMLNY RVPLNEIIYD FFDSLKSRTK GYASFDYELD 

       490        500        510        520        530        540 
GYMRTELVKL DIILNSEVVD ALSMIVPKDR AYAKGRTMAE KLKEIIPRQL FEIPIQAAVG 

       550        560        570        580        590        600 
SKIIARETVK ALRKDVLAKC YGGDISRKRK LLEKQKEGKK RMRQVGSVEV PQEAFMSILK 


ND

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References

[1]"Comparative genomic and transcriptomic analysis revealed genetic characteristics related to solvent formation and xylose utilization in Clostridium acetobutylicum EA 2018."
Hu S., Zheng H., Gu Y., Zhao J., Zhang W., Yang Y., Wang S., Zhao G., Yang S., Jiang W.
BMC Genomics 12:93-93(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EA 2018 EMBL ADZ20328.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002118 Genomic DNA. Translation: ADZ20328.1.
RefSeqYP_005670423.1. NC_017295.1.

3D structure databases

ProteinModelPortalF0KCN9.
SMRF0KCN9. Positions 6-553.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADZ20328; ADZ20328; CEA_G1291.
GeneID12233302.
KEGGcay:CEA_G1291.
PATRIC47109476. VBICloAce167171_1314.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK03596.
OMAKCYGGDA.

Family and domain databases

Gene3D3.30.70.240. 1 hit.
HAMAPMF_00071. LepA.
InterProIPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR005225. Small_GTP-bd_dom.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR009000. Transl_elong_init/rib_B-barrel.
[Graphical view]
PANTHERPTHR23115:SF40. PTHR23115:SF40. 1 hit.
PfamPF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMSSF54980. EFG_III_V. 2 hits.
SSF50447. Translat_factor. 1 hit.
TIGRFAMsTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF0KCN9_CLOAE
AccessionPrimary (citable) accession number: F0KCN9
Entry history
Integrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: May 1, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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