F0JHM7 (F0JHM7_DESDE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Cobyrinic acid A,C-diamide synthase HAMAP-Rule MF_00027

Gene names

Name:	cobB HAMAP-Rule MF_00027
ORF Names:	DND132_2058 EMBL EGB15263.1

Organism

Desulfovibrio desulfuricans ND132 EMBL EGB15263.1

Taxonomic identifier

641491 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio › Desulfovibrio desulfuricans

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation By similarity. HAMAP-Rule MF_00027
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis. HAMAP-Rule MF_00027
Sequence similarities	Belongs to the CobB/CobQ family. CobB subfamily. HAMAP-Rule MF_00027 Contains 1 GATase cobBQ-type domain. HAMAP-Rule MF_00027 SAAS SAAS011698

Ontologies

Keywords
Biological process	Cobalamin biosynthesis SAAS SAAS011698 HAMAP-Rule MF_00027
Domain	Glutamine amidotransferase SAAS SAAS011698 HAMAP-Rule MF_00027
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	253 – 448	196	GATase cobBQ-type By similarity HAMAP-Rule MF_00027
Sites
	Active site	336	1	Nucleophile By similarity HAMAP-Rule MF_00027
	Active site	440	1	By similarity HAMAP-Rule MF_00027

Sequences

	Sequence		Length	Mass (Da)
	F0JHM7 [UniParc]. Last modified May 3, 2011. Version 1. Checksum: 3CF537C442590DAF	FASTA	462	50,239
	10 20 30 40 50 60 MVSAVKGFVI AGTHSGCGKT SISLGLMASL ARRGRRVQPF KCGPDFIDPG HHALACAVDG 70 80 90 100 110 120 HPVPSHNLDG WMLDEATNLD LFNRYAAECD VAVIEGVMGL FDGISGTGDA GSTAQMAKIL 130 140 150 160 170 180 GLPVILVVDA RSMARSAAAL VAGYADFDPA VTIAGVIFNR VGSPAHAELL REAMTLVPDV 190 200 210 220 230 240 PVLGVLGRDE DISTPSRHLG LVTPEQDGPD MDRYQRLADW VETGLDPDAL LARLPEVEAV 250 260 270 280 290 300 PPFEPVPQLP RVTIGLARDD AFCFYYEENL RLLREAGARL IEFSPLNDPH LPEHLDGLYL 310 320 330 340 350 360 GGGYPELYVF ELGQNTRMRR DIKEFCESGR PVYAECGGFM LLMNDIITGR GRYAMAGVYP 370 380 390 400 410 420 VRAEMNEKFR ALGYREITAR EATLLGPAGT TARGHEFHYS SIQDHDPELL HPVYAMAGRK 430 440 450 460 GPLEEPEGFV TWNVLGSYVH LHFGSNPEIP KAFVKACMDV AR

References

[1]

"Genome sequence of the mercury-methylating strain Desulfovibrio desulfuricans ND132." Brown S.D., Gilmour C.C., Kucken A.M., Wall J.D., Elias D.A., Brandt C.C., Podar M., Chertkov O., Held B., Bruce D.C., Detter J.C., Tapia R., Han C.S., Goodwin L.A., Cheng J.F., Pitluck S., Woyke T., Mikhailova N. , Ivanova N.N., Han J., Lucas S., Lapidus A.L., Land M.L., Hauser L.J., Palumbo A.V. J. Bacteriol. 193:2078-2079(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ND132 EMBL EGB15263.1.

[2]

"Closure of Desulfovibrio sp. ND132." Hurt R.A.Jr., Elias D.A., Land M.L., Brown S.D. Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ND132 EMBL EGB15263.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP003220 Genomic DNA. Translation: EGB15263.1.
RefSeq	YP_005168066.1. NC_016803.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EGB15263; EGB15263; DND132_2058.
GeneID	11742195.
KEGG	ddn:DND132_2058.
PATRIC	46638150. VBIDesSp116210_2117.
Phylogenomic databases
KO	K02224.
Enzyme and pathway databases
UniPathway	UPA00148.
Family and domain databases
HAMAP	MF_00027. CobB.
InterPro	IPR004484. CbiA_synth. IPR017929. CobB/CobQ_GATase. IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom. IPR011698. GATase_3. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. PF07685. GATase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00379. cobB. 1 hit.
PROSITE	PS51274. GATASE_COBBQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F0JHM7_DESDE

Accession

Primary (citable) accession number: F0JHM7

Entry history

Integrated into UniProtKB/TrEMBL:	May 3, 2011
Last sequence update:	May 3, 2011
Last modified:	April 3, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)