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E9QYP0

- SIDA_ASPFU

UniProt

E9QYP0 - SIDA_ASPFU

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Protein

L-ornithine N(5)-monooxygenase

Gene

sidA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as the secreted triacetylfusarinine C (TAFC) involved in iron uptake and the intracellular iron storage compound desferriferricrocin (DFFC). Highly specific for its substrate, only hydrolyzing l-ornithine. Has preference for NADPH over NADH, NADPH playing a role in stabilization of the C4a-hydroperoxyflavin intermediate. Essential for virulence.4 Publications

Catalytic activityi

L-ornithine + NAD(P)H + O2 = N(5)-hydroxy-L-ornithine + NAD(P)+ + H2O.2 Publications

Cofactori

FAD3 PublicationsNote: Binds 1 FAD per subunit.3 Publications

Kineticsi

kcat is 29 min(-1) with L-ornithine as substrate and 75 min(-1) with NADPH as substrate.1 Publication

  1. KM=1.7 mM for L-ornithine (in the presence of 1 mM NADH)1 Publication
  2. KM=1.7 mM for L-ornithine (in the presence of 1 mM NADPH)1 Publication
  3. KM=0.49 mM for L-ornithine (at 37 degrees Celsius)1 Publication
  4. KM=0.58 mM for L-ornithine (at 25 degrees Celsius)1 Publication
  5. KM=0.94 mM for NADPH (in the presence of 15 mM L-ornithine)1 Publication
  6. KM=0.90 mM for NADH (in the presence of 15 mM L-ornithine)1 Publication
  7. KM=4.6 µM for NADPH (at 37 degrees Celsius)1 Publication
  8. KM=2.6 µM for NADPH (at 25 degrees Celsius)1 Publication
  9. KM=18 µM for O2 (at 37 degrees Celsius)1 Publication
  10. KM=16 µM for O2 (at 25 degrees Celsius)1 Publication

Pathwayi

Siderophore biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021FAD; via amide nitrogen1 Publication
Binding sitei107 – 1071Substrate1 Publication
Binding sitei168 – 1681FAD; via amide nitrogen and carbonyl oxygen1 Publication
Binding sitei279 – 2791NADP1 Publication
Binding sitei323 – 3231Substrate1 Publication
Binding sitei469 – 4691Substrate1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi83 – 919FAD1 Publication
Nucleotide bindingi254 – 2574NADP1 Publication
Nucleotide bindingi323 – 3253NADP1 Publication
Nucleotide bindingi466 – 4683FAD1 Publication

GO - Molecular functioni

  1. coenzyme binding Source: ASPGD
  2. iron ion binding Source: ASPGD
  3. monooxygenase activity Source: ASPGD
  4. N,N-dimethylaniline monooxygenase activity Source: ASPGD
  5. NADP+ binding Source: ASPGD

GO - Biological processi

  1. cellular iron ion homeostasis Source: ASPGD
  2. cellular response to iron ion starvation Source: ASPGD
  3. ergosterol biosynthetic process Source: ASPGD
  4. iron assimilation Source: ASPGD
  5. pathogenesis Source: ASPGD
  6. secondary metabolite biosynthetic process Source: ASPGD
  7. siderophore biosynthetic process Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00783.

Names & Taxonomyi

Protein namesi
Recommended name:
L-ornithine N(5)-monooxygenase1 Publication (EC:1.14.13.1962 Publications)
Short name:
OMOBy similarity
Alternative name(s):
L-ornithine N(5)-oxygenase1 Publication
Siderphore biosynthesis protein A1 Publication
Gene namesi
Name:sidA1 Publication
ORF Names:Afu2g07680
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000002530: Chromosome 2

Pathology & Biotechi

Disruption phenotypei

Moderatley reduced growth rate during iron starvation and in iron replete conditions. Only displays 1% of wild-type conidiospore production in iron-depleted and replete conditions. Completely attenuates virulence in a mouse model of invasive pulmonary aspergillosis.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501L-ornithine N(5)-monooxygenasePRO_0000431070Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4B63X-ray1.90A1-501[»]
4B64X-ray2.28A1-501[»]
4B65X-ray2.32A1-501[»]
4B66X-ray2.90A1-501[»]
4B67X-ray2.75A1-501[»]
4B68X-ray2.29A1-501[»]
4B69X-ray2.30A1-501[»]
ProteinModelPortaliE9QYP0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni293 – 2964Substrate binding1 Publication

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000237193.
KOiK10531.
OMAiFPSRHEF.
OrthoDBiEOG7C8GS3.

Family and domain databases

InterProiIPR025700. Lys/Orn_oxygenase.
[Graphical view]
PfamiPF13434. K_oxygenase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E9QYP0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESVERKSES SYLGMRNMQP EQRLSLDPPR LRSTPQDELH DLLCVGFGPA
60 70 80 90 100
SLAIAIALHD ALDPRLNKSA SNIHAQPKIC FLERQKQFAW HSGMLVPGSK
110 120 130 140 150
MQISFIKDLA TLRDPRSSFT FLNYLHQKGR LIHFTNLSTF LPARLEFEDY
160 170 180 190 200
MRWCAQQFSD VVAYGEEVVE VIPGKSDPSS SVVDFFTVRS RNVETGEISA
210 220 230 240 250
RRTRKVVIAI GGTAKMPSGL PQDPRIIHSS KYCTTLPALL KDKSKPYNIA
260 270 280 290 300
VLGSGQSAAE IFHDLQKRYP NSRTTLIMRD SAMRPSDDSP FVNEIFNPER
310 320 330 340 350
VDKFYSQSAA ERQRSLLADK ATNYSVVRLE LIEEIYNDMY LQRVKNPDET
360 370 380 390 400
QWQHRILPER KITRVEHHGP QSRMRIHLKS SKPESEGAAN DVKETLEVDA
410 420 430 440 450
LMVATGYNRN AHERLLSKVQ HLRPTGQDQW KPHRDYRVEM DPSKVSSEAG
460 470 480 490 500
IWLQGCNERT HGLSDSLLSV LAVRGGEMVQ SIFGEQLERA AVQGHQLRAM

L
Length:501
Mass (Da):56,877
Last modified:April 5, 2011 - v1
Checksum:iDD1D4DEDD2509506
GO

Mass spectrometryi

Molecular mass is 57210 Da from positions 1 - 501. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY586511 Genomic DNA. Translation: AAT84594.1.
AY819708 Genomic DNA. Translation: AAX40989.1.
AAHF01000001 Genomic DNA. Translation: EAL93065.1.
RefSeqiXP_755103.1. XM_750010.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00004182; CADAFUAP00004182; CADAFUAG00004182.
GeneIDi3513640.
KEGGiafm:AFUA_2G07680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY586511 Genomic DNA. Translation: AAT84594.1 .
AY819708 Genomic DNA. Translation: AAX40989.1 .
AAHF01000001 Genomic DNA. Translation: EAL93065.1 .
RefSeqi XP_755103.1. XM_750010.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4B63 X-ray 1.90 A 1-501 [» ]
4B64 X-ray 2.28 A 1-501 [» ]
4B65 X-ray 2.32 A 1-501 [» ]
4B66 X-ray 2.90 A 1-501 [» ]
4B67 X-ray 2.75 A 1-501 [» ]
4B68 X-ray 2.29 A 1-501 [» ]
4B69 X-ray 2.30 A 1-501 [» ]
ProteinModelPortali E9QYP0.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADAFUAT00004182 ; CADAFUAP00004182 ; CADAFUAG00004182 .
GeneIDi 3513640.
KEGGi afm:AFUA_2G07680.

Phylogenomic databases

HOGENOMi HOG000237193.
KOi K10531.
OMAi FPSRHEF.
OrthoDBi EOG7C8GS3.

Enzyme and pathway databases

UniPathwayi UPA00783 .

Family and domain databases

InterProi IPR025700. Lys/Orn_oxygenase.
[Graphical view ]
Pfami PF13434. K_oxygenase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Siderophore biosynthesis but not reductive iron assimilation is essential for Aspergillus fumigatus virulence."
    Schrettl M., Bignell E., Kragl C., Joechl C., Rogers T., Arst H.N. Jr., Haynes K., Haas H.
    J. Exp. Med. 200:1213-1219(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PATHWAY, DISRUPTION PHENOTYPE.
  2. "The Aspergillus fumigatus siderophore biosynthetic gene sidA, encoding L-ornithine N5-oxygenase, is required for virulence."
    Hissen A.H., Wan A.N., Warwas M.L., Pinto L.J., Moore M.M.
    Infect. Immun. 73:5493-5503(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
    Strain: NIH 5233 / ATCC 13073.
  3. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
  4. "Aspergillus fumigatus SidA is a highly specific ornithine hydroxylase with bound flavin cofactor."
    Chocklett S.W., Sobrado P.
    Biochemistry 49:6777-6783(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, PATHWAY, SUBUNIT, MASS SPECTROMETRY.
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
  5. "Comprehensive spectroscopic, steady state, and transient kinetic studies of a representative siderophore-associated flavin monooxygenase."
    Mayfield J.A., Frederick R.E., Streit B.R., Wencewicz T.A., Ballou D.P., DuBois J.L.
    J. Biol. Chem. 285:30375-30388(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR.
  6. "Dual role of NADP(H) in the reaction of a flavin dependent N-hydroxylating monooxygenase."
    Romero E., Fedkenheuer M., Chocklett S.W., Qi J., Oppenheimer M., Sobrado P.
    Biochim. Biophys. Acta 1824:850-857(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Structural insight into the mechanism of oxygen activation and substrate selectivity of flavin-dependent N-hydroxylating monooxygenases."
    Franceschini S., Fedkenheuer M., Vogelaar N.J., Robinson H.H., Sobrado P., Mattevi A.
    Biochemistry 51:7043-7045(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH FAD; NADP; L-ORNITHINE; LYSINE AND L-ARGININE.

Entry informationi

Entry nameiSIDA_ASPFU
AccessioniPrimary (citable) accession number: E9QYP0
Secondary accession number(s): Q4X250, Q5SE95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 26, 2014
Last sequence update: April 5, 2011
Last modified: November 26, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3