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Protein

Kelch-like protein 40b

Gene

klhl40b

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

ubstrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex (By similarity). Required for skeletal muscle development (PubMed:23746549).By similarity1 Publication

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processUbl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Kelch-like protein 40b
Alternative name(s):
Kelch repeat and BTB domain-containing protein 5b
Gene namesi
Name:klhl40b
Synonyms:kbtbd5b
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 24

Organism-specific databases

ZFINiZDB-GENE-060227-1. klhl40b.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Morpholino knockdown of the protein results in a curved trunk and small head at 48 hpf. Morphants show disruption of skeletal muscle patterning with an irregular, wavy appearance of the striated myofibers and extensive gaps between the myofibers. Myofibers show disorganized and irregular patterns with small aggregates of alpha-actinin, suggesting nemaline bodies. Animals exhibit sporadic muscle tremor and coordinated swimming is not observed.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004238641 – 618Kelch-like protein 40bAdd BLAST618

Proteomic databases

PaxDbiE9QJ30.
PeptideAtlasiE9QJ30.
PRIDEiE9QJ30.

Expressioni

Tissue specificityi

Expressed in skeletal muscle. Detected in the eye at much lower levels.1 Publication

Developmental stagei

At 16 and 24 hpf, restricted to muscle precursor cells in somites. Also detected at 48 hpf.1 Publication

Gene expression databases

BgeeiENSDARG00000019125.

Interactioni

Subunit structurei

Component of the BCR(KLHL40) E3 ubiquitin ligase complex.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000012991.

Structurei

3D structure databases

SMRiE9QJ30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 100BTBPROSITE-ProRule annotationAdd BLAST68
Domaini135 – 237BACKAdd BLAST103
Repeati356 – 408Kelch 1Add BLAST53
Repeati409 – 458Kelch 2Add BLAST50
Repeati459 – 506Kelch 3Add BLAST48
Repeati508 – 553Kelch 4Add BLAST46
Repeati555 – 608Kelch 5Add BLAST54

Sequence similaritiesi

Belongs to the KLHL40 family.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00910000143979.
InParanoidiE9QJ30.
KOiK10473.
OMAiFLINDSA.
OrthoDBiEOG091G0C3Y.
PhylomeDBiE9QJ30.
TreeFamiTF351653.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
InterProiView protein in InterPro
IPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR030607. KLHL40.
IPR011333. SKP1/BTB/POZ_sf.
PANTHERiPTHR24412:SF22. PTHR24412:SF22. 1 hit.
PfamiView protein in Pfam
PF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 3 hits.
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiView protein in SMART
SM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 4 hits.
SUPFAMiSSF117281. SSF117281. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiView protein in PROSITE
PS50097. BTB. 1 hit.

Sequencei

Sequence statusi: Complete.

E9QJ30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPIDPMEE PRMYQQTLLQ DGLYDLLESD MMVDCVLKIK DKEFPCHRLV
60 70 80 90 100
LAACSSYFRA FFKSGVEESK QREIVLEDVE PGVMGIILKY LYTSNINVTE
110 120 130 140 150
QNVQDIFALS NMLQIPSIFT VCVSFLQKRL SLSNCLAIFR LGLMLDCPRL
160 170 180 190 200
AISARNFACE RFQFITRDEE FLQLTPSELA AVLASDSLNV ETEQDVFEAL
210 220 230 240 250
IKWVGHDQEN RIGDLPDLLD CIRLRLVPRD YFVKNVEKHE WLSSNPEITK
260 270 280 290 300
KLQLVKDAHA GKLPELKKTK NKKSPSEEGQ KKGDEEEVEE EEEQEERLPG
310 320 330 340 350
ILNDNLRFGM FLRELIFLIN DSASVAYDPT GNDCYVASVS TQIPKNHCSL
360 370 380 390 400
VTKENQIFVA GGLFFDEQSK DEQIYSYFLQ FDPASSDWMG MPPIPSPRFL
410 420 430 440 450
FGMGEAENFI FVIGGREMKE GENILNTVMV YDRQFLKWAE SDPLPYLVYG
460 470 480 490 500
HGVVSHNEMI YVIGGKGENK ECLNRVCAYD IKTHQWKDLA PLNTARSLFG
510 520 530 540 550
VTIHKNNIYV VAGVTDSGLT GSAEVYDIKT NKWSEFVEFP QDRSSLSLVS
560 570 580 590 600
VSGVLYAVGG FAMFPKEDSD DLMPLEMNDI WRYDESERTW SGILRENRYA
610
SGATVLGVRL NTLRLTKM
Length:618
Mass (Da):70,347
Last modified:April 5, 2011 - v1
Checksum:i885AFA940D17A862
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571770 Genomic DNA. No translation available.
RefSeqiXP_001334076.1. XM_001334040.6.
UniGeneiDr.134110.

Genome annotation databases

EnsembliENSDART00000010488; ENSDARP00000012991; ENSDARG00000019125.
GeneIDi795319.
KEGGidre:795319.

Similar proteinsi

Entry informationi

Entry nameiKL40B_DANRE
AccessioniPrimary (citable) accession number: E9QJ30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: April 5, 2011
Last modified: February 28, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome