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Protein

Zinc finger protein 431

Gene

Znf431

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific DNA binding transcriptional repressor. Represses target gene transcription by recruiting HDAC1 and HDAC2 histone deacetylases. Acts as a specific transcriptional repressor for PTCH1 during embryonic development. Required for osteoblast differentiation and sonic hedgehog/SHH signaling response. Binds to the consensus site 5'-GCGCCC-3' in the promoter of PTCH1.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri104 – 12623C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri132 – 15423C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri160 – 18223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri188 – 21023C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri216 – 23823C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri244 – 26623C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri272 – 29423C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri300 – 32223C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri328 – 35023C2H2-type 9PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri356 – 37823C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri384 – 40623C2H2-type 11PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri412 – 43423C2H2-type 12PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri440 – 46223C2H2-type 13PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri468 – 49023C2H2-type 14PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri496 – 51823C2H2-type 15PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • core promoter sequence-specific DNA binding Source: UniProtKB
  • histone deacetylase binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 431
Alternative name(s):
Zinc finger protein 932
Gene namesi
Name:Znf431
Synonyms:Zfp431, Zfp932
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1916754. Zfp932.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526Zinc finger protein 431PRO_0000425265Add
BLAST

Proteomic databases

PaxDbiE9QAG8.
PRIDEiE9QAG8.

Expressioni

Tissue specificityi

Expressed in brain, heart, lung, thymus, spleen, lymph node, liver, kidney, muscle, testis, ovary, skin and uterus.1 Publication

Developmental stagei

Expressed in limb mesenchyme at 10.5 dpc. Expressed in tooth, submandibular glands, thymus, thyroid, vibrissa follicles at 14.5 dpc.2 Publications

Gene expression databases

BgeeiE9QAG8.
ExpressionAtlasiE9QAG8. baseline and differential.
GenevisibleiE9QAG8. MM.

Interactioni

Subunit structurei

Interacts (via KRAB domain) with HDAC2; the interaction is direct. Interacts (via KRAB domain) with HDAC1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Hdac1O091063EBI-9549639,EBI-301912
Hdac2P702883EBI-9549639,EBI-302251

GO - Molecular functioni

  • histone deacetylase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi213490. 2 interactions.
IntActiE9QAG8. 2 interactions.
STRINGi10090.ENSMUSP00000108159.

Structurei

3D structure databases

ProteinModelPortaliE9QAG8.
SMRiE9QAG8. Positions 4-60, 64-524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7672KRABPROSITE-ProRule annotationAdd
BLAST

Domaini

The KRAB domain is necessary for its repressive activity.

Sequence similaritiesi

Contains 15 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KRAB domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri104 – 12623C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri132 – 15423C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri160 – 18223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri188 – 21023C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri216 – 23823C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri244 – 26623C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri272 – 29423C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri300 – 32223C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri328 – 35023C2H2-type 9PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri356 – 37823C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri384 – 40623C2H2-type 11PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri412 – 43423C2H2-type 12PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri440 – 46223C2H2-type 13PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri468 – 49023C2H2-type 14PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri496 – 51823C2H2-type 15PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000119089.
InParanoidiE9QAG8.
OMAiSGHIKEH.
OrthoDBiEOG7KSX7Q.
TreeFamiTF338854.

Family and domain databases

Gene3Di3.30.160.60. 16 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 3 hits.
PF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 15 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 15 hits.
PS50157. ZINC_FINGER_C2H2_2. 15 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E9QAG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDALTYDDV YVNFTQEEWA LLNPSQKSLY KDVMLETYRN LNAVGYNWED
60 70 80 90 100
SNIEEHCESS RRHGRHERNH TGEKPYEGIQ YGEAFVHHSS LQMRKIIHTG
110 120 130 140 150
EKRYKCNQCD KAYSRHSILQ IHKRTHSGEK PYECNQCGKA FTQHSHLKIH
160 170 180 190 200
MVTHTGEKPY KCDQCGKAFA FHSTLQVHKR THTGEKPYEC NQCSKAFAHH
210 220 230 240 250
CHLRVHKRIH TGEKPYKCDQ CGKAFVGQND LKRHERVHTG EKPYKCNECG
260 270 280 290 300
KAFVCNASLR THKTTHTGVK PYECKQCTKS FASHGQLQKH ERIHTGEKPY
310 320 330 340 350
KCDQCGKAFA SHDKFQKHER IHIGEKPYKC KQCTKSFASH DKLQKHERIH
360 370 380 390 400
TGEKPYECKQ CTKSFASHNK LQKHERIHTG EKPYKCDQCN KAFVYESYLQ
410 420 430 440 450
VHKKTHTGEK PYKCNECGKA FARHSHLKVH KITHTGEKPY KCNQCGKALA
460 470 480 490 500
YHSTLQVHQR THTGEKPYEC EQCGKAFANQ SYFQVHKRIH TGEKPYKCDQ
510 520
CGKAFVGSSD LKRHERVHTG RETLQM
Length:526
Mass (Da):61,280
Last modified:April 5, 2011 - v1
Checksum:i1F7F44631FEB1BA2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti323 – 3231I → T in BC012405 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC123679 Genomic DNA. No translation available.
BC012405 mRNA. No translation available.
CCDSiCCDS59681.1.
RefSeqiNP_663538.2. NM_145563.2.
UniGeneiMm.441438.

Genome annotation databases

EnsembliENSMUST00000112540; ENSMUSP00000108159; ENSMUSG00000066613.
GeneIDi69504.
KEGGimmu:69504.
UCSCiuc008ypn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC123679 Genomic DNA. No translation available.
BC012405 mRNA. No translation available.
CCDSiCCDS59681.1.
RefSeqiNP_663538.2. NM_145563.2.
UniGeneiMm.441438.

3D structure databases

ProteinModelPortaliE9QAG8.
SMRiE9QAG8. Positions 4-60, 64-524.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213490. 2 interactions.
IntActiE9QAG8. 2 interactions.
STRINGi10090.ENSMUSP00000108159.

Proteomic databases

PaxDbiE9QAG8.
PRIDEiE9QAG8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112540; ENSMUSP00000108159; ENSMUSG00000066613.
GeneIDi69504.
KEGGimmu:69504.
UCSCiuc008ypn.1. mouse.

Organism-specific databases

CTDi69504.
MGIiMGI:1916754. Zfp932.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000119089.
InParanoidiE9QAG8.
OMAiSGHIKEH.
OrthoDBiEOG7KSX7Q.
TreeFamiTF338854.

Miscellaneous databases

NextBioi329614.
PROiE9QAG8.
SOURCEiSearch...

Gene expression databases

BgeeiE9QAG8.
ExpressionAtlasiE9QAG8. baseline and differential.
GenevisibleiE9QAG8. MM.

Family and domain databases

Gene3Di3.30.160.60. 16 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 3 hits.
PF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 15 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 15 hits.
PS50157. ZINC_FINGER_C2H2_2. 15 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "A novel KRAB domain-containing zinc finger transcription factor ZNF431 directly represses Patched1 transcription."
    He Z., Cai J., Lim J.W., Kroll K., Ma L.
    J. Biol. Chem. 286:7279-7289(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HDAC1 AND HDAC2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  4. "ZFP932 suppresses cellular Hedgehog response and Patched1 transcription."
    Huang G.J., He Z., Ma L.
    Vitam. Horm. 88:309-332(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HDAC2, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiZN431_MOUSE
AccessioniPrimary (citable) accession number: E9QAG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: April 5, 2011
Last modified: May 11, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.