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Protein

2'-5'-oligoadenylate synthase 2

Gene

Oas2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L.1 Publication

Catalytic activityi

3 ATP = pppA2'p5'A2'p5'A + 2 diphosphate.

Cofactori

Mg2+Curated

Enzyme regulationi

Produced as a latent enzyme which is activated by dsRNA generated during the course of viral infection. The dsRNA activator must be at least 15 nucleotides long, and no modification of the 2'-hydroxyl group is tolerated. ssRNA or dsDNA do not act as activators (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi448 – 4481Magnesium; catalyticSequence analysis
Metal bindingi450 – 4501Magnesium; catalyticSequence analysis
Binding sitei461 – 4611SubstrateBy similarity
Metal bindingi519 – 5191Magnesium; catalyticSequence analysis
Binding sitei582 – 5821ATPBy similarity
Binding sitei582 – 5821SubstrateBy similarity

GO - Molecular functioni

  • 2'-5'-oligoadenylate synthetase activity Source: MGI
  • ATP binding Source: UniProtKB
  • double-stranded RNA binding Source: MGI
  • zinc ion binding Source: MGI

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • protein glycosylation Source: UniProtKB
  • protein myristoylation Source: UniProtKB
  • purine nucleotide biosynthetic process Source: MGI
  • response to virus Source: UniProtKB
  • RNA catabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
2'-5'-oligoadenylate synthase 2 (EC:2.7.7.84)
Short name:
(2-5')oligo(A) synthase 2
Short name:
2-5A synthase 2
Alternative name(s):
2',5'-oligoadenylate synthetase-like 11
Gene namesi
Name:Oas2
Synonyms:oasl11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2180852. Oas2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microsome, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 7427412'-5'-oligoadenylate synthase 2PRO_0000418628Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei417 – 4171N6-acetyllysineBy similarity

Post-translational modificationi

Myristoylation is not essential for its activity.By similarity
Glycosylated. Glycosylation is essential for its activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Lipoprotein, Myristate

Proteomic databases

EPDiE9Q9A9.
MaxQBiE9Q9A9.
PaxDbiE9Q9A9.
PRIDEiE9Q9A9.

PTM databases

iPTMnetiE9Q9A9.

Expressioni

Tissue specificityi

Uterus.1 Publication

Gene expression databases

BgeeiE9Q9A9.
ExpressionAtlasiE9Q9A9. baseline and differential.
GenevisibleiE9Q9A9. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060082.

Structurei

3D structure databases

ProteinModelPortaliE9Q9A9.
SMRiE9Q9A9. Positions 59-361, 382-718.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 374315OAS domain 1Add
BLAST
Regioni382 – 721340OAS domain 2Add
BLAST

Sequence similaritiesi

Belongs to the 2-5A synthase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00510000046406.
HOGENOMiHOG000013200.
HOVERGENiHBG007855.
InParanoidiE9Q9A9.
KOiK14216.
OMAiKSYTSQK.
OrthoDBiEOG7WDN1R.
TreeFamiTF329749.

Family and domain databases

Gene3Di1.10.1410.20. 2 hits.
InterProiIPR006117. 2-5-oligoadenylate_synth_CS.
IPR006116. 2-5-oligoadenylate_synth_N.
IPR018952. 2-5-oligoAdlate_synth_1_dom2/C.
IPR026774. 2-5A_synthase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR11258. PTHR11258. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF10421. OAS1_C. 2 hits.
[Graphical view]
PROSITEiPS00832. 25A_SYNTH_1. 1 hit.
PS00833. 25A_SYNTH_2. 2 hits.
PS50152. 25A_SYNTH_3. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: E9Q9A9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNWLTGNWS SDRSSGYSSG WSPGGSSGVP SGPVHKLEKS IQANLTPNEN
60 70 80 90 100
CLKQIAVSSV PSQKLEGYIQ ENLKPNRESL KQIDQAVDAI WDLLRSQIPV
110 120 130 140 150
KEVAKGGSYG RETALRGCSD GTLVLFMDCF QQFQDQIKYQ DAYLDVIELW
160 170 180 190 200
LKIHEKKSVK HEHALVVQVS VPGQRILLQL LPVFNPLRSN ENPSSCVYVD
210 220 230 240 250
LKKSMDQVRA SPGEFSDCFT TLQQRFFKKY PQRLKDLILL VKHWYEQCQE
260 270 280 290 300
KWKTPPPQPL LYALELLTVY AWEQGCQAED FDMAQGVRTV LRLIQRPTEL
310 320 330 340 350
CVYWTVNYNF EDETVRNILL HQLRSQRPVI LDPTDPTNNV GKDDGFWELL
360 370 380 390 400
TEEAMAWLYS PSLNTESPAP YWDVLPMPLF VTPSHLLNKF IKDFLQPNKL
410 420 430 440 450
FLKQIKEAVD IICSFLKNVC FLNSDTKVLK TVKGGSTAKG TALKRGSDAD
460 470 480 490 500
IVVFLSSLES YDSLKTNRSQ FVQEIQKQLE EFVQAQEWEV TFEISKWKAP
510 520 530 540 550
RVLSFTLKSK TLNESVEFDV LPAYDALGQL RSDFTLRPEA YKDLIELCAS
560 570 580 590 600
QDIKEGEFSI CFTELQRNFI QTRPTKLKSL LRLIKHWYKQ YERKMKPKAS
610 620 630 640 650
LPPKYALELL TVYAWEQGSG TDDFDIAEGF RTVLDLVIKY RQLCIFWTVN
660 670 680 690 700
YNFEEEYMRK FLLTQIQKKR PVILDPADPT GDVGGGDRWC WHLLAEEAKE
710 720 730 740
WLSSPCFQVE QKGLVQPWKV PVMQTPGSCG GQIYPTVGGV TK
Length:742
Mass (Da):85,051
Last modified:April 5, 2011 - v1
Checksum:iF8F21FB4BEF90C58
GO
Isoform 2 (identifier: E9Q9A9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     723-742: MQTPGSCGGQIYPTVGGVTK → PRDLKTSDMVGVFTTGGILWQDQGFLSFV

Show »
Length:751
Mass (Da):86,285
Checksum:iB4E650AE78280C49
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti270 – 2701Y → C in BAB84135 (PubMed:12396720).Curated
Sequence conflicti630 – 6301F → L in BAB84135 (PubMed:12396720).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti232 – 2321Q → R in strain: C3H/He. 2 Publications

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei723 – 74220MQTPG…GGVTK → PRDLKTSDMVGVFTTGGILW QDQGFLSFV in isoform 2. 1 PublicationVSP_044068Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB067535 mRNA. Translation: BAB84135.1.
AF418010 mRNA. Translation: AAM54499.1.
AC115937 Genomic DNA. No translation available.
CH466529 Genomic DNA. Translation: EDL19753.1.
CCDSiCCDS19625.2. [E9Q9A9-1]
RefSeqiNP_660262.2. NM_145227.3. [E9Q9A9-1]
XP_006530388.1. XM_006530325.2. [E9Q9A9-2]
UniGeneiMm.260926.

Genome annotation databases

EnsembliENSMUST00000053909; ENSMUSP00000060082; ENSMUSG00000032690. [E9Q9A9-1]
ENSMUST00000081491; ENSMUSP00000080209; ENSMUSG00000032690. [E9Q9A9-2]
GeneIDi246728.
KEGGimmu:246728.
UCSCiuc008zhw.2. mouse. [E9Q9A9-2]
uc008zhx.2. mouse. [E9Q9A9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB067535 mRNA. Translation: BAB84135.1.
AF418010 mRNA. Translation: AAM54499.1.
AC115937 Genomic DNA. No translation available.
CH466529 Genomic DNA. Translation: EDL19753.1.
CCDSiCCDS19625.2. [E9Q9A9-1]
RefSeqiNP_660262.2. NM_145227.3. [E9Q9A9-1]
XP_006530388.1. XM_006530325.2. [E9Q9A9-2]
UniGeneiMm.260926.

3D structure databases

ProteinModelPortaliE9Q9A9.
SMRiE9Q9A9. Positions 59-361, 382-718.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060082.

PTM databases

iPTMnetiE9Q9A9.

Proteomic databases

EPDiE9Q9A9.
MaxQBiE9Q9A9.
PaxDbiE9Q9A9.
PRIDEiE9Q9A9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053909; ENSMUSP00000060082; ENSMUSG00000032690. [E9Q9A9-1]
ENSMUST00000081491; ENSMUSP00000080209; ENSMUSG00000032690. [E9Q9A9-2]
GeneIDi246728.
KEGGimmu:246728.
UCSCiuc008zhw.2. mouse. [E9Q9A9-2]
uc008zhx.2. mouse. [E9Q9A9-1]

Organism-specific databases

CTDi4939.
MGIiMGI:2180852. Oas2.

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00510000046406.
HOGENOMiHOG000013200.
HOVERGENiHBG007855.
InParanoidiE9Q9A9.
KOiK14216.
OMAiKSYTSQK.
OrthoDBiEOG7WDN1R.
TreeFamiTF329749.

Miscellaneous databases

ChiTaRSiOas2. mouse.
PROiE9Q9A9.
SOURCEiSearch...

Gene expression databases

BgeeiE9Q9A9.
ExpressionAtlasiE9Q9A9. baseline and differential.
GenevisibleiE9Q9A9. MM.

Family and domain databases

Gene3Di1.10.1410.20. 2 hits.
InterProiIPR006117. 2-5-oligoadenylate_synth_CS.
IPR006116. 2-5-oligoadenylate_synth_N.
IPR018952. 2-5-oligoAdlate_synth_1_dom2/C.
IPR026774. 2-5A_synthase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR11258. PTHR11258. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF10421. OAS1_C. 2 hits.
[Graphical view]
PROSITEiPS00832. 25A_SYNTH_1. 1 hit.
PS00833. 25A_SYNTH_2. 2 hits.
PS50152. 25A_SYNTH_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic structure of the mouse 2',5'-oligoadenylate synthetase gene family."
    Kakuta S., Shibata S., Iwakura Y.
    J. Interferon Cytokine Res. 22:981-993(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Strain: C57BL/6J.
    Tissue: Colon.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT ARG-232.
    Strain: C3H/He.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-232.
  5. "The mammalian 2'-5' oligoadenylate synthetase gene family: evidence for concerted evolution of paralogous Oas1 genes in Rodentia and Artiodactyla."
    Perelygin A.A., Zharkikh A.A., Scherbik S.V., Brinton M.A.
    J. Mol. Evol. 63:562-576(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  6. "The oligoadenylate synthetase family: an ancient protein family with multiple antiviral activities."
    Kristiansen H., Gad H.H., Eskildsen-Larsen S., Despres P., Hartmann R.
    J. Interferon Cytokine Res. 31:41-47(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON FUNCTION.

Entry informationi

Entry nameiOAS2_MOUSE
AccessioniPrimary (citable) accession number: E9Q9A9
Secondary accession number(s): Q8K4E5, Q8VI92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: April 5, 2011
Last modified: June 8, 2016
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.