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Protein

Ubiquitin conjugation factor E4 A

Gene

Ube4a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-ubiquitin ligase activity Source: MGI

GO - Biological processi

  • protein polyubiquitination Source: MGI
  • ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin conjugation factor E4 ACurated (EC:6.3.2.-1 Publication)
Gene namesi
Name:Ube4aImported
Synonyms:Ufd2bImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2154580. Ube4a.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • ubiquitin ligase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10281028Ubiquitin conjugation factor E4 APRO_0000430791Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei386 – 3861N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiE9Q735.
PaxDbiE9Q735.
PeptideAtlasiE9Q735.
PRIDEiE9Q735.

PTM databases

PhosphoSiteiE9Q735.

Expressioni

Tissue specificityi

Expressed in liver, heart, brain, kidney and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000059890.
ExpressionAtlasiE9Q735. baseline and differential.
GenevisibleiE9Q735. MM.

Interactioni

Protein-protein interaction databases

BioGridi228292. 3 interactions.
STRINGi10090.ENSMUSP00000113346.

Structurei

3D structure databases

ProteinModelPortaliE9Q735.
SMRiE9Q735. Positions 939-1023.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini949 – 102375U-boxPROSITE-ProRule annotationAdd
BLAST

Domaini

The U-box domain is required for the ubiquitin protein ligase activity.By similarity

Sequence similaritiesi

Contains 1 U-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2042. Eukaryota.
COG5113. LUCA.
GeneTreeiENSGT00390000009300.
KOiK10596.
TreeFamiTF300802.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR019474. Ub_conjug_fac_E4_core.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF04564. U-box. 1 hit.
PF10408. Ufd2P_core. 2 hits.
[Graphical view]
SMARTiSM00504. Ubox. 1 hit.
[Graphical view]
PROSITEiPS51698. U_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E9Q735-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDQENNNNI SSNPFAALFG SLADAKQFAA IQKEQLKQQS DELPASPDDS
60 70 80 90 100
DNSVSESLDE FDYSVSEISR SFRTHQEMCE QLNINHMIQR IFLITLDNSD
110 120 130 140 150
PSLKSGNGIP SRCVYLEEMA VELEDQDWLD MSNVEQAIFA RLLLQDPGNH
160 170 180 190 200
LISMTSSTTL NLSADRDAGE RHIFCYLYSC FQRAKEEITK VPENLLPFAV
210 220 230 240 250
QCRNLTVSNT RTVLLTPEIY VDQNIHEQLV DLMLEAIQGA HFEDVTEFLE
260 270 280 290 300
EVIEALLLDE EVRTFPEVMI PVFDILLSRI KDLELCQILL YAYLDILLYF
310 320 330 340 350
TRQKDMAKVF LEYIQPKDPS NGQMYQKTLL GVILNISCLL KTPGVVENHG
360 370 380 390 400
FFLNPSRSSP QEIKVQEANI HQFMAQFHEK IYQMLKNLLQ LSPETKHCIL
410 420 430 440 450
FWLGNCLHAN AGRTKIWANQ MPEIFFQMYA SDAFFLNLGA ALLKLCQPFC
460 470 480 490 500
KPRSSRLLTF NPTYCVLKDL NDEERKIKSV HMRGLDKETC LIPAVQEPTF
510 520 530 540 550
PQSYNLVTEN LALTEYTLYL GFHRLHDQMV KINQNLHRLQ VAWRDAQQSS
560 570 580 590 600
SPAADNLREQ FERLMTIYLS TKTAMTEPQM LQNCLNLQVS MAVLLVQLAI
610 620 630 640 650
GNEGSQPIEL SFPLPDGYSS LAYVPEFFAD NLGDFLIFLR RFAEDILETS
660 670 680 690 700
ADSLEHVLHF ITIFTGSIER MKNPHLRAKL AEVLEAVMPH LDQTPSPLVS
710 720 730 740 750
SVFHRKRVFC NFPYAPQLAE ALIKVFVDIE FTGDPHQFEQ KFNYRRPMYP
760 770 780 790 800
ILRYMWGTDC YRESIKYLSK IKIQQIEKDR GEWESLTPEA RREKEAGLQM
810 820 830 840 850
FGQLARFHNI MSNETIGTLS FLTSEIKSLF VHPFLAERII SMLNYFLQHL
860 870 880 890 900
VGPKMGALKV KDFSEFDFKP QQLVSDICTI YLNLGDEENF CATVPKDGRS
910 920 930 940 950
YSPTLFAQTV RVLKKINKPG NMIVAFSNLA ERIKSLADLQ QQEEETYADA
960 970 980 990 1000
CDEFLDPIMS TLMSDPVVLP SSRVTVDRST IARHLLSDQT DPFNRSPLTM
1010 1020
DQIRPNTELK EKIQRWLAER KQQKEQPE
Length:1,028
Mass (Da):118,198
Last modified:April 5, 2011 - v1
Checksum:i00221784ECDD8761
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC061963 Genomic DNA. No translation available.
CCDSiCCDS23122.2.
RefSeqiNP_663375.3. NM_145400.3.
UniGeneiMm.215069.
Mm.421524.

Genome annotation databases

EnsembliENSMUST00000117506; ENSMUSP00000113346; ENSMUSG00000059890.
GeneIDi140630.
KEGGimmu:140630.
UCSCiuc009peu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC061963 Genomic DNA. No translation available.
CCDSiCCDS23122.2.
RefSeqiNP_663375.3. NM_145400.3.
UniGeneiMm.215069.
Mm.421524.

3D structure databases

ProteinModelPortaliE9Q735.
SMRiE9Q735. Positions 939-1023.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228292. 3 interactions.
STRINGi10090.ENSMUSP00000113346.

PTM databases

PhosphoSiteiE9Q735.

Proteomic databases

MaxQBiE9Q735.
PaxDbiE9Q735.
PeptideAtlasiE9Q735.
PRIDEiE9Q735.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000117506; ENSMUSP00000113346; ENSMUSG00000059890.
GeneIDi140630.
KEGGimmu:140630.
UCSCiuc009peu.2. mouse.

Organism-specific databases

CTDi9354.
MGIiMGI:2154580. Ube4a.

Phylogenomic databases

eggNOGiKOG2042. Eukaryota.
COG5113. LUCA.
GeneTreeiENSGT00390000009300.
KOiK10596.
TreeFamiTF300802.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiUbe4a. mouse.
PROiE9Q735.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000059890.
ExpressionAtlasiE9Q735. baseline and differential.
GenevisibleiE9Q735. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR019474. Ub_conjug_fac_E4_core.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF04564. U-box. 1 hit.
PF10408. Ufd2P_core. 2 hits.
[Graphical view]
SMARTiSM00504. Ubox. 1 hit.
[Graphical view]
PROSITEiPS51698. U_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBE4A_MOUSE
AccessioniPrimary (citable) accession number: E9Q735
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 29, 2014
Last sequence update: April 5, 2011
Last modified: September 7, 2016
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.