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Protein

Unconventional myosin-Ie

Gene

Myo1e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. Binds to membranes containing anionic phospholipids via its tail domain (By similarity). Required for normal morphology of the glomerular basement membrane, normal development of foot processes by kidney podocytes and normal kidney function. In dendritic cells, may control the movement of class II-containing cytoplasmic vesicles along the actin cytoskeleton by connecting them with the actin network via ARL14EP and ARL14 (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi112 – 119ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • endocytosis Source: UniProtKB
  • glomerular basement membrane development Source: UniProtKB
  • glomerular filtration Source: UniProtKB
  • glomerular visceral epithelial cell development Source: UniProtKB
  • hemopoiesis Source: MGI
  • in utero embryonic development Source: MGI
  • kidney development Source: MGI
  • nitrogen compound metabolic process Source: MGI
  • platelet-derived growth factor receptor signaling pathway Source: MGI
  • post-embryonic hemopoiesis Source: MGI
  • vasculogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Lipid-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-Ie
Alternative name(s):
Unconventional myosin 1E
Gene namesi
Name:Myo1e
Synonyms:Myr3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:106621. Myo1e.

Subcellular locationi

GO - Cellular componenti

  • adherens junction Source: UniProtKB
  • brush border Source: UniProtKB
  • cell-cell junction Source: UniProtKB
  • clathrin-coated endocytic vesicle Source: Ensembl
  • cytoplasm Source: UniProtKB
  • cytoskeleton Source: UniProtKB
  • extracellular exosome Source: MGI
  • myosin complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

No visible phenotype at birth. Mice exhibit massive proteinuria, combined with the presence of leukocytes and hemoglobin in the urine. They develop enlarged kidneys, present damage to the glomeruli, renal inflammation and fibrosis. In the glomeruli, the thickness of the basement membrane is increased, and podocytes fail to develop normal foot processes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004156641 – 1107Unconventional myosin-IeAdd BLAST1107

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1001PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiE9Q634.
MaxQBiE9Q634.
PaxDbiE9Q634.
PeptideAtlasiE9Q634.
PRIDEiE9Q634.

PTM databases

iPTMnetiE9Q634.
PhosphoSitePlusiE9Q634.

Expressioni

Tissue specificityi

Detected in kidney glomeruli (at protein level). Detected in utricle.2 Publications

Gene expression databases

BgeeiENSMUSG00000032220.
GenevisibleiE9Q634. MM.

Interactioni

Subunit structurei

Interacts with CALM and F-actin. Interacts (via SH3 domain) with SYNJ1, DNM1 and DNM2. Interacts with ARL14EP. Interacts with CARMIL1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi214807. 3 interactors.
IntActiE9Q634. 1 interactor.
STRINGi10090.ENSMUSP00000034745.

Structurei

Secondary structure

11107
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1054 – 1059Combined sources6
Beta strandi1076 – 1082Combined sources7
Beta strandi1086 – 1092Combined sources7
Beta strandi1095 – 1100Combined sources6
Helixi1101 – 1103Combined sources3
Beta strandi1104 – 1106Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XMFX-ray1.50A1053-1107[»]
ProteinModelPortaliE9Q634.
SMRiE9Q634.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 692Myosin motorAdd BLAST674
Domaini695 – 724IQPROSITE-ProRule annotationAdd BLAST30
Domaini730 – 922TH1PROSITE-ProRule annotationAdd BLAST193
Domaini1050 – 1107SH3PROSITE-ProRule annotationAdd BLAST58

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni581 – 591Actin-bindingSequence analysisAdd BLAST11

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 TH1 (class I myosin tail homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG0164. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129697.
HOGENOMiHOG000260265.
HOVERGENiHBG100702.
InParanoidiE9Q634.
KOiK10356.
OMAiATWPSWQ.
OrthoDBiEOG091G0136.
TreeFamiTF312960.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR027417. P-loop_NTPase.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
PR00452. SH3DOMAIN.
SMARTiSM00242. MYSc. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
PS50002. SH3. 1 hit.
PS51757. TH1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E9Q634-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSKGAYRYH WQSHNVKHSG VDDMVLLSKI TESSIVENLK KRYMDDYIFT
60 70 80 90 100
YIGSVLISVN PFKQMPYFGE KEVEMYQGAA QYENPPHIYA LADSMYRNMI
110 120 130 140 150
IDRENQCVII SGESGAGKTV AAKYIMSYVS RVSGGGPKVQ HVKDIILQSN
160 170 180 190 200
PLLEAFGNAK TVRNNNSSRF GKYFEIQFSP GGEPDGGKIS NFLLEKSRVV
210 220 230 240 250
MRNPGERSFH IFYQLIEGAS PEQKQSLGIT SMDYYYYLSL SGSYKVDDID
260 270 280 290 300
DKRDFQETLH AMNVIGIFSE EQTLVLQIVA GILHLGNISF KEVGNYAAVE
310 320 330 340 350
SEEFLAFPAY LLGINQDRLK EKLTSRQMDS KWGGKSESIH VTLNVEQACY
360 370 380 390 400
TRDALAKALH ARVFDFLVDS INKAMEKDHE EYNIGVLDIY GFEIFQKNGF
410 420 430 440 450
EQFCINFVNE KLQQIFIELT LKAEQEEYVQ EGIRWTPIEY FNNKIVCDLI
460 470 480 490 500
ESKVNPPGIM SILDDVCATM HAVGEGADQT LLQKLQMQIG SHEHFNSWNQ
510 520 530 540 550
GFIIHHYAGK VSYDMDGFCE RNRDVLFMDL IELMQSSELP FIKSLFPENL
560 570 580 590 600
QADKKGRPTT AGSKIKKQAN DLVSTLMKCT PHYIRCIKPN ETKKPKDWEE
610 620 630 640 650
SRVKHQVEYL GLKENIRVRR AGYAYRRVFQ KFLQRYAILT KATWPVWRGD
660 670 680 690 700
EKQGVLHLLQ SVNMDSDQFQ LGRSKVFIKA PESLFLLEEM RERKYDGYAR
710 720 730 740 750
VIQKTWRKFV ARKKYVQMRE EASDLLLNKK ERRRNSINRN FIGDYIGMEE
760 770 780 790 800
RPELQQFVGK REKIDFADTV TKYDRRFKGV KRDLLLTPKC LYLIGREKVK
810 820 830 840 850
QGPDKGVVKE VLKRRIEVER ILSVSLSTMQ DDIFILHEQE YDSLLESVFK
860 870 880 890 900
TEFLSLLAKR YEEKTQKQLP LKFSNTLELK LKKENWGPWS AGGSRQVQFH
910 920 930 940 950
QGFGDLAILK PSNKVLQVSI GPGLPKNSRP TRRNTVTSRG YPGGTKNNYP
960 970 980 990 1000
MRAAPAPPGC HQNGVIRNQF VPPPHAFGNQ RSNQKSLYTS MARPPLPRQQ
1010 1020 1030 1040 1050
STGSDRLSQT PESLDFLKVP DQGVAGVRRQ TSSRPPPAGG RPKPQPKPKP
1060 1070 1080 1090 1100
QVPQCKALYA YDAQDTDELS FNANDIIDII KEDPSGWWTG RLRGKQGLFP

NNYVTKI
Length:1,107
Mass (Da):126,818
Last modified:April 5, 2011 - v1
Checksum:iD745BE859E2F079D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73V → I in AAH51391 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC157086 Genomic DNA. No translation available.
AC157950 Genomic DNA. No translation available.
AC157996 Genomic DNA. No translation available.
BC051391 mRNA. Translation: AAH51391.1.
AF426465 mRNA. Translation: AAL26545.1.
CCDSiCCDS40680.1.
RefSeqiNP_851417.2. NM_181072.3.
UniGeneiMm.249311.
Mm.444670.

Genome annotation databases

EnsembliENSMUST00000034745; ENSMUSP00000034745; ENSMUSG00000032220.
GeneIDi71602.
KEGGimmu:71602.
UCSCiuc009qnx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC157086 Genomic DNA. No translation available.
AC157950 Genomic DNA. No translation available.
AC157996 Genomic DNA. No translation available.
BC051391 mRNA. Translation: AAH51391.1.
AF426465 mRNA. Translation: AAL26545.1.
CCDSiCCDS40680.1.
RefSeqiNP_851417.2. NM_181072.3.
UniGeneiMm.249311.
Mm.444670.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XMFX-ray1.50A1053-1107[»]
ProteinModelPortaliE9Q634.
SMRiE9Q634.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214807. 3 interactors.
IntActiE9Q634. 1 interactor.
STRINGi10090.ENSMUSP00000034745.

PTM databases

iPTMnetiE9Q634.
PhosphoSitePlusiE9Q634.

Proteomic databases

EPDiE9Q634.
MaxQBiE9Q634.
PaxDbiE9Q634.
PeptideAtlasiE9Q634.
PRIDEiE9Q634.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034745; ENSMUSP00000034745; ENSMUSG00000032220.
GeneIDi71602.
KEGGimmu:71602.
UCSCiuc009qnx.1. mouse.

Organism-specific databases

CTDi4643.
MGIiMGI:106621. Myo1e.

Phylogenomic databases

eggNOGiKOG0164. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129697.
HOGENOMiHOG000260265.
HOVERGENiHBG100702.
InParanoidiE9Q634.
KOiK10356.
OMAiATWPSWQ.
OrthoDBiEOG091G0136.
TreeFamiTF312960.

Miscellaneous databases

ChiTaRSiMyo1e. mouse.
PROiE9Q634.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032220.
GenevisibleiE9Q634. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR027417. P-loop_NTPase.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
PR00452. SH3DOMAIN.
SMARTiSM00242. MYSc. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
PS50002. SH3. 1 hit.
PS51757. TH1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYO1E_MOUSE
AccessioniPrimary (citable) accession number: E9Q634
Secondary accession number(s): Q80X36, Q91ZI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: April 5, 2011
Last modified: November 30, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-1 (MYH1).Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.