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E9Q634

- MYO1E_MOUSE

UniProt

E9Q634 - MYO1E_MOUSE

Protein

Unconventional myosin-Ie

Gene

Myo1e

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (05 Apr 2011)
      Previous versions | rss
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    Functioni

    Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. Binds to membranes containing anionic phospholipids via its tail domain By similarity. Required for normal morphology of the glomerular basement membrane, normal development of foot processes by kidney podocytes and normal kidney function. In dendritic cells, may control the movement of class II-containing cytoplasmic vesicles along the actin cytoskeleton by connecting them with the actin network via ARL14EP and ARL14 By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi112 – 1198ATPSequence Analysis

    GO - Molecular functioni

    1. actin filament binding Source: UniProtKB
    2. ATPase activity, coupled Source: UniProtKB
    3. ATP binding Source: UniProtKB-KW
    4. calmodulin binding Source: UniProtKB
    5. motor activity Source: InterPro
    6. phosphatidylinositol binding Source: UniProtKB

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. endocytosis Source: UniProtKB
    3. glomerular basement membrane development Source: UniProtKB
    4. glomerular filtration Source: UniProtKB
    5. glomerular visceral epithelial cell development Source: UniProtKB
    6. hemopoiesis Source: MGI
    7. in utero embryonic development Source: MGI
    8. kidney development Source: MGI
    9. nitrogen compound metabolic process Source: MGI
    10. platelet-derived growth factor receptor signaling pathway Source: MGI
    11. post-embryonic hemopoiesis Source: MGI
    12. vasculogenesis Source: MGI

    Keywords - Molecular functioni

    Motor protein, Myosin

    Keywords - Ligandi

    Actin-binding, ATP-binding, Calmodulin-binding, Lipid-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Unconventional myosin-Ie
    Alternative name(s):
    Unconventional myosin 1E
    Gene namesi
    Name:Myo1e
    Synonyms:Myr3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:106621. Myo1e.

    Subcellular locationi

    Cytoplasm 1 Publication. Cell junction 1 Publication. Cytoplasmic vesicle 1 Publication. Cytoplasmic vesicleclathrin-coated vesicle 1 Publication. Cytoplasmcytoskeleton 1 Publication
    Note: In podocytes, it localizes close to and is associated with the cytoplasmic membrane, with enrichment at the lamellipodia tips. Colocalizes with F-actin By similarity. Detected in cytoplasmic punctae.By similarity

    GO - Cellular componenti

    1. adherens junction Source: UniProtKB
    2. cell-cell junction Source: UniProtKB
    3. clathrin-coated endocytic vesicle Source: Ensembl
    4. cytoplasm Source: UniProtKB
    5. cytoskeleton Source: UniProtKB
    6. myosin complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype at birth. Mice exhibit massive proteinuria, combined with the presence of leukocytes and hemoglobin in the urine. They develop enlarged kidneys, present damage to the glomeruli, renal inflammation and fibrosis. In the glomeruli, the thickness of the basement membrane is increased, and podocytes fail to develop normal foot processes.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11071107Unconventional myosin-IePRO_0000415664Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1001 – 10011PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiE9Q634.
    PRIDEiE9Q634.

    PTM databases

    PhosphoSiteiE9Q634.

    Expressioni

    Tissue specificityi

    Detected in kidney glomeruli (at protein level). Detected in utricle.2 Publications

    Gene expression databases

    ArrayExpressiE9Q634.
    BgeeiE9Q634.

    Interactioni

    Subunit structurei

    Interacts with CALM and F-actin. Interacts (via SH3 domain) with SYNJ1, DNM1 and DNM2 By similarity. Interacts with ARL14EP By similarity.By similarity

    Structurei

    Secondary structure

    1
    1107
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi1054 – 10596
    Beta strandi1076 – 10827
    Beta strandi1086 – 10927
    Beta strandi1095 – 11006
    Helixi1101 – 11033
    Beta strandi1104 – 11063

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2XMFX-ray1.50A1053-1107[»]
    ProteinModelPortaliE9Q634.
    SMRiE9Q634. Positions 20-692, 1053-1107.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 692674Myosin motorAdd
    BLAST
    Domaini695 – 72430IQPROSITE-ProRule annotationAdd
    BLAST
    Domaini1050 – 110758SH3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni581 – 59111Actin-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 IQ domain.PROSITE-ProRule annotation
    Contains 1 myosin motor domain.Curated
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    GeneTreeiENSGT00750000117217.
    HOGENOMiHOG000260265.
    HOVERGENiHBG100702.
    KOiK10356.
    OMAiKLKKENW.
    OrthoDBiEOG7V49XQ.
    TreeFamiTF312960.

    Family and domain databases

    InterProiIPR000048. IQ_motif_EF-hand-BS.
    IPR001609. Myosin_head_motor_dom.
    IPR010926. Myosin_tail_2.
    IPR027417. P-loop_NTPase.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00063. Myosin_head. 1 hit.
    PF06017. Myosin_TH1. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00193. MYOSINHEAVY.
    PR00452. SH3DOMAIN.
    SMARTiSM00242. MYSc. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50096. IQ. 1 hit.
    PS51456. MYOSIN_MOTOR. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E9Q634-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSKGAYRYH WQSHNVKHSG VDDMVLLSKI TESSIVENLK KRYMDDYIFT     50
    YIGSVLISVN PFKQMPYFGE KEVEMYQGAA QYENPPHIYA LADSMYRNMI 100
    IDRENQCVII SGESGAGKTV AAKYIMSYVS RVSGGGPKVQ HVKDIILQSN 150
    PLLEAFGNAK TVRNNNSSRF GKYFEIQFSP GGEPDGGKIS NFLLEKSRVV 200
    MRNPGERSFH IFYQLIEGAS PEQKQSLGIT SMDYYYYLSL SGSYKVDDID 250
    DKRDFQETLH AMNVIGIFSE EQTLVLQIVA GILHLGNISF KEVGNYAAVE 300
    SEEFLAFPAY LLGINQDRLK EKLTSRQMDS KWGGKSESIH VTLNVEQACY 350
    TRDALAKALH ARVFDFLVDS INKAMEKDHE EYNIGVLDIY GFEIFQKNGF 400
    EQFCINFVNE KLQQIFIELT LKAEQEEYVQ EGIRWTPIEY FNNKIVCDLI 450
    ESKVNPPGIM SILDDVCATM HAVGEGADQT LLQKLQMQIG SHEHFNSWNQ 500
    GFIIHHYAGK VSYDMDGFCE RNRDVLFMDL IELMQSSELP FIKSLFPENL 550
    QADKKGRPTT AGSKIKKQAN DLVSTLMKCT PHYIRCIKPN ETKKPKDWEE 600
    SRVKHQVEYL GLKENIRVRR AGYAYRRVFQ KFLQRYAILT KATWPVWRGD 650
    EKQGVLHLLQ SVNMDSDQFQ LGRSKVFIKA PESLFLLEEM RERKYDGYAR 700
    VIQKTWRKFV ARKKYVQMRE EASDLLLNKK ERRRNSINRN FIGDYIGMEE 750
    RPELQQFVGK REKIDFADTV TKYDRRFKGV KRDLLLTPKC LYLIGREKVK 800
    QGPDKGVVKE VLKRRIEVER ILSVSLSTMQ DDIFILHEQE YDSLLESVFK 850
    TEFLSLLAKR YEEKTQKQLP LKFSNTLELK LKKENWGPWS AGGSRQVQFH 900
    QGFGDLAILK PSNKVLQVSI GPGLPKNSRP TRRNTVTSRG YPGGTKNNYP 950
    MRAAPAPPGC HQNGVIRNQF VPPPHAFGNQ RSNQKSLYTS MARPPLPRQQ 1000
    STGSDRLSQT PESLDFLKVP DQGVAGVRRQ TSSRPPPAGG RPKPQPKPKP 1050
    QVPQCKALYA YDAQDTDELS FNANDIIDII KEDPSGWWTG RLRGKQGLFP 1100
    NNYVTKI 1107
    Length:1,107
    Mass (Da):126,818
    Last modified:April 5, 2011 - v1
    Checksum:iD745BE859E2F079D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti73 – 731V → I in AAH51391. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC157086 Genomic DNA. No translation available.
    AC157950 Genomic DNA. No translation available.
    AC157996 Genomic DNA. No translation available.
    BC051391 mRNA. Translation: AAH51391.1.
    AF426465 mRNA. Translation: AAL26545.1.
    CCDSiCCDS40680.1.
    RefSeqiNP_851417.2. NM_181072.3.
    UniGeneiMm.249311.

    Genome annotation databases

    EnsembliENSMUST00000034745; ENSMUSP00000034745; ENSMUSG00000032220.
    GeneIDi71602.
    KEGGimmu:71602.
    UCSCiuc009qnx.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC157086 Genomic DNA. No translation available.
    AC157950 Genomic DNA. No translation available.
    AC157996 Genomic DNA. No translation available.
    BC051391 mRNA. Translation: AAH51391.1 .
    AF426465 mRNA. Translation: AAL26545.1 .
    CCDSi CCDS40680.1.
    RefSeqi NP_851417.2. NM_181072.3.
    UniGenei Mm.249311.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2XMF X-ray 1.50 A 1053-1107 [» ]
    ProteinModelPortali E9Q634.
    SMRi E9Q634. Positions 20-692, 1053-1107.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei E9Q634.

    Proteomic databases

    MaxQBi E9Q634.
    PRIDEi E9Q634.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034745 ; ENSMUSP00000034745 ; ENSMUSG00000032220 .
    GeneIDi 71602.
    KEGGi mmu:71602.
    UCSCi uc009qnx.1. mouse.

    Organism-specific databases

    CTDi 4643.
    MGIi MGI:106621. Myo1e.

    Phylogenomic databases

    GeneTreei ENSGT00750000117217.
    HOGENOMi HOG000260265.
    HOVERGENi HBG100702.
    KOi K10356.
    OMAi KLKKENW.
    OrthoDBi EOG7V49XQ.
    TreeFami TF312960.

    Miscellaneous databases

    ChiTaRSi MYO1E. mouse.
    NextBioi 334081.
    PROi E9Q634.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi E9Q634.
    Bgeei E9Q634.

    Family and domain databases

    InterProi IPR000048. IQ_motif_EF-hand-BS.
    IPR001609. Myosin_head_motor_dom.
    IPR010926. Myosin_tail_2.
    IPR027417. P-loop_NTPase.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00063. Myosin_head. 1 hit.
    PF06017. Myosin_TH1. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00193. MYOSINHEAVY.
    PR00452. SH3DOMAIN.
    SMARTi SM00242. MYSc. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50096. IQ. 1 hit.
    PS51456. MYOSIN_MOTOR. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    3. "Myosin-I isozymes in neonatal rodent auditory and vestibular epithelia."
      Dumont R.A., Zhao Y.-D., Holt J.R., Baehler M., Gillespie P.G.
      J. Assoc. Res. Otolaryngol. 3:375-389(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 600-726, TISSUE SPECIFICITY.
      Strain: C57BL/6.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. "Myosin 1E SH3 domain."
      Allsop G., Harris S.A., Peckham M., Edwards T.
      Submitted (AUG-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 1053-1107.

    Entry informationi

    Entry nameiMYO1E_MOUSE
    AccessioniPrimary (citable) accession number: E9Q634
    Secondary accession number(s): Q80X36, Q91ZI4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: April 5, 2011
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Represents an unconventional myosin. This protein should not be confused with the conventional myosin-1 (MYH1).Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3