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E9Q634 (MYO1E_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Unconventional myosin-Ie
Alternative name(s):
Unconventional myosin 1E
Gene names
Name:Myo1e
Synonyms:Myr3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1107 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. Binds to membranes containing anionic phospholipids via its tail domain By similarity. Required for normal morphology of the glomerular basement membrane, normal development of foot processes by kidney podocytes and normal kidney function. In dendritic cells, may control the movement of class II-containing cytoplasmic vesicles along the actin cytoskeleton by connecting them with the actin network via ARL14EP and ARL14 By similarity. Ref.5

Subunit structure

Interacts with CALM and F-actin. Interacts (via SH3 domain) with SYNJ1, DNM1 and DNM2 By similarity. Interacts with ARL14EP By similarity.

Subcellular location

Cytoplasm. Cell junction. Cytoplasmic vesicle Probable. Cytoplasmic vesicleclathrin-coated vesicle. Cytoplasmcytoskeleton. Note: In podocytes, it localizes close to and is associated with the cytoplasmic membrane, with enrichment at the lamellipodia tips. Colocalizes with F-actin By similarity. Detected in cytoplasmic punctae. Ref.5

Tissue specificity

Detected in kidney glomeruli (at protein level). Detected in utricle. Ref.3 Ref.5

Disruption phenotype

No visible phenotype at birth. Mice exhibit massive proteinuria, combined with the presence of leukocytes and hemoglobin in the urine. They develop enlarged kidneys, present damage to the glomeruli, renal inflammation and fibrosis. In the glomeruli, the thickness of the basement membrane is increased, and podocytes fail to develop normal foot processes. Ref.5

Sequence similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Contains 1 IQ domain.

Contains 1 myosin motor domain.

Contains 1 SH3 domain.

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-1 (MYH1).

Ontologies

Keywords
   Cellular componentCell junction
Cytoplasm
Cytoplasmic vesicle
Cytoskeleton
   DomainSH3 domain
   LigandActin-binding
ATP-binding
Calmodulin-binding
Lipid-binding
Nucleotide-binding
   Molecular functionMotor protein
Myosin
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP catabolic process

Inferred from sequence or structural similarity. Source: GOC

endocytosis

Inferred from sequence or structural similarity. Source: UniProtKB

glomerular basement membrane development

Inferred from mutant phenotype Ref.5. Source: UniProtKB

glomerular filtration

Inferred from mutant phenotype Ref.5. Source: UniProtKB

glomerular visceral epithelial cell development

Inferred from mutant phenotype Ref.5. Source: UniProtKB

hemopoiesis

Inferred from mutant phenotype PubMed 17143286. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 17143286. Source: MGI

kidney development

Inferred from mutant phenotype PubMed 17143286. Source: MGI

nitrogen compound metabolic process

Inferred from mutant phenotype PubMed 17143286. Source: MGI

platelet-derived growth factor receptor signaling pathway

Inferred from mutant phenotype PubMed 17143286. Source: MGI

post-embryonic hemopoiesis

Inferred from mutant phenotype PubMed 17143286. Source: MGI

vasculogenesis

Inferred from mutant phenotype PubMed 17143286. Source: MGI

   Cellular_componentadherens junction

Inferred from direct assay Ref.5. Source: UniProtKB

cell-cell junction

Inferred from direct assay Ref.5. Source: UniProtKB

clathrin-coated endocytic vesicle

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from direct assay Ref.5. Source: UniProtKB

cytoskeleton

Inferred from sequence or structural similarity. Source: UniProtKB

myosin complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATPase activity, coupled

Inferred from sequence or structural similarity. Source: UniProtKB

actin filament binding

Inferred from sequence or structural similarity. Source: UniProtKB

calmodulin binding

Inferred from sequence or structural similarity. Source: UniProtKB

motor activity

Inferred from electronic annotation. Source: InterPro

phosphatidylinositol binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11071107Unconventional myosin-Ie
PRO_0000415664

Regions

Domain19 – 692674Myosin motor
Domain695 – 72430IQ
Domain1050 – 110758SH3
Nucleotide binding112 – 1198ATP Potential
Region581 – 59111Actin-binding Potential

Amino acid modifications

Modified residue10011Phosphoserine By similarity

Experimental info

Sequence conflict731V → I in AAH51391. Ref.2

Secondary structure

........... 1107
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
E9Q634 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: D745BE859E2F079D

FASTA1,107126,818
        10         20         30         40         50         60 
MGSKGAYRYH WQSHNVKHSG VDDMVLLSKI TESSIVENLK KRYMDDYIFT YIGSVLISVN 

        70         80         90        100        110        120 
PFKQMPYFGE KEVEMYQGAA QYENPPHIYA LADSMYRNMI IDRENQCVII SGESGAGKTV 

       130        140        150        160        170        180 
AAKYIMSYVS RVSGGGPKVQ HVKDIILQSN PLLEAFGNAK TVRNNNSSRF GKYFEIQFSP 

       190        200        210        220        230        240 
GGEPDGGKIS NFLLEKSRVV MRNPGERSFH IFYQLIEGAS PEQKQSLGIT SMDYYYYLSL 

       250        260        270        280        290        300 
SGSYKVDDID DKRDFQETLH AMNVIGIFSE EQTLVLQIVA GILHLGNISF KEVGNYAAVE 

       310        320        330        340        350        360 
SEEFLAFPAY LLGINQDRLK EKLTSRQMDS KWGGKSESIH VTLNVEQACY TRDALAKALH 

       370        380        390        400        410        420 
ARVFDFLVDS INKAMEKDHE EYNIGVLDIY GFEIFQKNGF EQFCINFVNE KLQQIFIELT 

       430        440        450        460        470        480 
LKAEQEEYVQ EGIRWTPIEY FNNKIVCDLI ESKVNPPGIM SILDDVCATM HAVGEGADQT 

       490        500        510        520        530        540 
LLQKLQMQIG SHEHFNSWNQ GFIIHHYAGK VSYDMDGFCE RNRDVLFMDL IELMQSSELP 

       550        560        570        580        590        600 
FIKSLFPENL QADKKGRPTT AGSKIKKQAN DLVSTLMKCT PHYIRCIKPN ETKKPKDWEE 

       610        620        630        640        650        660 
SRVKHQVEYL GLKENIRVRR AGYAYRRVFQ KFLQRYAILT KATWPVWRGD EKQGVLHLLQ 

       670        680        690        700        710        720 
SVNMDSDQFQ LGRSKVFIKA PESLFLLEEM RERKYDGYAR VIQKTWRKFV ARKKYVQMRE 

       730        740        750        760        770        780 
EASDLLLNKK ERRRNSINRN FIGDYIGMEE RPELQQFVGK REKIDFADTV TKYDRRFKGV 

       790        800        810        820        830        840 
KRDLLLTPKC LYLIGREKVK QGPDKGVVKE VLKRRIEVER ILSVSLSTMQ DDIFILHEQE 

       850        860        870        880        890        900 
YDSLLESVFK TEFLSLLAKR YEEKTQKQLP LKFSNTLELK LKKENWGPWS AGGSRQVQFH 

       910        920        930        940        950        960 
QGFGDLAILK PSNKVLQVSI GPGLPKNSRP TRRNTVTSRG YPGGTKNNYP MRAAPAPPGC 

       970        980        990       1000       1010       1020 
HQNGVIRNQF VPPPHAFGNQ RSNQKSLYTS MARPPLPRQQ STGSDRLSQT PESLDFLKVP 

      1030       1040       1050       1060       1070       1080 
DQGVAGVRRQ TSSRPPPAGG RPKPQPKPKP QVPQCKALYA YDAQDTDELS FNANDIIDII 

      1090       1100 
KEDPSGWWTG RLRGKQGLFP NNYVTKI 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[3]"Myosin-I isozymes in neonatal rodent auditory and vestibular epithelia."
Dumont R.A., Zhao Y.-D., Holt J.R., Baehler M., Gillespie P.G.
J. Assoc. Res. Otolaryngol. 3:375-389(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 600-726, TISSUE SPECIFICITY.
Strain: C57BL/6.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]"Disruption of Myosin 1e promotes podocyte injury."
Krendel M., Kim S.V., Willinger T., Wang T., Kashgarian M., Flavell R.A., Mooseker M.S.
J. Am. Soc. Nephrol. 20:86-94(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]"Myosin 1E SH3 domain."
Allsop G., Harris S.A., Peckham M., Edwards T.
Submitted (AUG-2011) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 1053-1107.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC157086 Genomic DNA. No translation available.
AC157950 Genomic DNA. No translation available.
AC157996 Genomic DNA. No translation available.
BC051391 mRNA. Translation: AAH51391.1.
AF426465 mRNA. Translation: AAL26545.1.
CCDSCCDS40680.1.
RefSeqNP_851417.2. NM_181072.3.
UniGeneMm.249311.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2XMFX-ray1.50A1053-1107[»]
ProteinModelPortalE9Q634.
SMRE9Q634. Positions 20-692, 1053-1107.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteE9Q634.

Proteomic databases

MaxQBE9Q634.
PRIDEE9Q634.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034745; ENSMUSP00000034745; ENSMUSG00000032220.
GeneID71602.
KEGGmmu:71602.
UCSCuc009qnx.1. mouse.

Organism-specific databases

CTD4643.
MGIMGI:106621. Myo1e.

Phylogenomic databases

GeneTreeENSGT00750000117217.
HOGENOMHOG000260265.
HOVERGENHBG100702.
KOK10356.
OMAKLKKENW.
OrthoDBEOG7V49XQ.
TreeFamTF312960.

Gene expression databases

ArrayExpressE9Q634.
BgeeE9Q634.

Family and domain databases

InterProIPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_tail_2.
IPR027417. P-loop_NTPase.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00193. MYOSINHEAVY.
PR00452. SH3DOMAIN.
SMARTSM00242. MYSc. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMYO1E. mouse.
NextBio334081.
PROE9Q634.
SOURCESearch...

Entry information

Entry nameMYO1E_MOUSE
AccessionPrimary (citable) accession number: E9Q634
Secondary accession number(s): Q80X36, Q91ZI4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: April 5, 2011
Last modified: July 9, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot