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Protein

Receptor-type tyrosine-protein phosphatase O

Gene

Ptpro

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possesses tyrosine phosphatase activity. Plays a role in regulating the glomerular pressure/filtration rate relationship through an effect on podocyte structure and function.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1112SubstrateBy similarity1
Active sitei1146Phosphocysteine intermediatePROSITE-ProRule annotation1
Binding sitei1190SubstrateBy similarity1

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • protein tyrosine phosphatase activity Source: UniProtKB
  • Wnt-protein binding Source: UniProtKB

GO - Biological processi

  • axon guidance Source: UniProtKB
  • cell morphogenesis Source: UniProtKB
  • glomerular visceral epithelial cell differentiation Source: UniProtKB
  • glomerulus development Source: UniProtKB
  • lamellipodium assembly Source: UniProtKB
  • monocyte chemotaxis Source: UniProtKB
  • negative regulation of canonical Wnt signaling pathway Source: UniProtKB
  • negative regulation of glomerular filtration Source: UniProtKB
  • peptidyl-tyrosine dephosphorylation Source: UniProtKB
  • regulation of glomerular filtration Source: UniProtKB
  • slit diaphragm assembly Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protein phosphatase, Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase O (EC:3.1.3.48)
Short name:
R-PTP-O
Alternative name(s):
Glomerular epithelial protein 1
Protein tyrosine phosphatase U2
Short name:
PTP-U2
Short name:
PTPase U2
Gene namesi
Name:Ptpro
Synonyms:GLEPP1, Ptpn15, PTPU2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1097152 Ptpro

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 832ExtracellularSequence analysisAdd BLAST803
Transmembranei833 – 853HelicalSequence analysisAdd BLAST21
Topological domaini854 – 1226CytoplasmicSequence analysisAdd BLAST373

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Modification of podocyte structure such that the normal octopoid podocyte is simplified to a more amoeboid structure and that the foot processes are shorter and broader than normal. These changes are associated with altered distribution of the podocyte intermediate cytoskeletal protein vimentin/VIM. Mutant animals have a reduced glomerular filtration rate and reduced glomerular nephrin (NPHS1) content. However, there is no evidence of proteinuria. After removal of one or more kidneys, Ptpro-null animals have higher blood pressure than does their wild-type littermates.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1220Y → F: Loss of tyrosine phosphorylation. Abolishes interaction with FYN and GRB2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000041405930 – 1226Receptor-type tyrosine-protein phosphatase OAdd BLAST1197

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi154N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi227N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi279N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi471N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi500N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi710N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi743N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi800N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei875PhosphoserineCombined sources1
Modified residuei1220Phosphotyrosine1 Publication1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiE9Q612
PaxDbiE9Q612
PRIDEiE9Q612

PTM databases

iPTMnetiE9Q612
PhosphoSitePlusiE9Q612

Expressioni

Gene expression databases

BgeeiENSMUSG00000030223
ExpressionAtlasiE9Q612 baseline and differential
GenevisibleiE9Q612 MM

Interactioni

Subunit structurei

Interacts (phosphorylated form) with FYN and GRB2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Wnt3aP274672EBI-8183885,EBI-2899665

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • Wnt-protein binding Source: UniProtKB

Protein-protein interaction databases

IntActiE9Q612 1 interactor.
MINTiE9Q612
STRINGi10090.ENSMUSP00000076364

Structurei

3D structure databases

ProteinModelPortaliE9Q612
SMRiE9Q612
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 115Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST86
Domaini339 – 435Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST97
Domaini445 – 541Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST97
Domaini542 – 638Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST97
Domaini641 – 734Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST94
Domaini735 – 827Fibronectin type-III 6PROSITE-ProRule annotationAdd BLAST93
Domaini948 – 1205Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST258

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1146 – 1152Substrate bindingBy similarity7

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0791 Eukaryota
COG5599 LUCA
GeneTreeiENSGT00900000140785
HOGENOMiHOG000115792
HOVERGENiHBG053763
InParanoidiE9Q612
KOiK18035
OMAiSVTACTE
OrthoDBiEOG091G00JF
TreeFamiTF351926

Family and domain databases

CDDicd00063 FN3, 3 hits
Gene3Di2.60.40.103 hits
3.90.190.101 hit
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR029021 Prot-tyrosine_phosphatase-like
IPR000242 PTPase_domain
IPR016130 Tyr_Pase_AS
IPR003595 Tyr_Pase_cat
IPR000387 TYR_PHOSPHATASE_dom
PfamiView protein in Pfam
PF00041 fn3, 2 hits
PF00102 Y_phosphatase, 1 hit
PRINTSiPR00700 PRTYPHPHTASE
SMARTiView protein in SMART
SM00060 FN3, 4 hits
SM00194 PTPc, 1 hit
SM00404 PTPc_motif, 1 hit
SUPFAMiSSF49265 SSF49265, 3 hits
SSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 5 hits
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50055 TYR_PHOSPHATASE_PTP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E9Q612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHLPRGTLG GRRLLPLLGL FVLLKIVTTF HVAVQDDNNI VVSLEASDIV
60 70 80 90 100
SPASVYVVRV AGESKNYFFE FEEFNSTLPP PVVFKATYHG LYYIITLVVV
110 120 130 140 150
NGNVVTKPSR SITVLTKPLP VTSVSIYDYK PSPETGVLFE IHYPEKYNVF
160 170 180 190 200
SRVNISYWEG RDFRTMLYKD FFKGKTVFNH WLPGLCYSNI TFQLVSEATF
210 220 230 240 250
NKSTLVEYSG VSHEPKQHRT APYPPRNISV RFVNLNKNNW EEPSGSFPED
260 270 280 290 300
SFIKPPQDSI GRDRRFHFPE ETPETPPSNV SSGSPPSNVS SAWPDPNSTD
310 320 330 340 350
YESTSQPFWW DSASAAPENE EDFVSALPAD YDTETTLDRT EKPTADPFSA
360 370 380 390 400
FPVQMTLSWL PPKPPTAFDG FNILIEREEN FTDYLTVDEE AHEFVAELKE
410 420 430 440 450
PGKYKLSVTT FSSSGACETR KSQSAKSLSF YISPTGEWIE ELTEKPQHVS
460 470 480 490 500
VHVLSSTTAL MSWTSSQENY NSTIVSVVSL TCQKQKESQR LEKQYCTQVN
510 520 530 540 550
SSKPVIENLV PGAQYQVVMY LRKGPLIGPP SDPVTFAIVP TGIKDLMLYP
560 570 580 590 600
LGPTAVVLSW TRPILGVFRK YVVEMFYFNP TTMTSEWTTY YEIAATVSLT
610 620 630 640 650
ASVRIASLLP AWYYNFRVTM VTWGDPELSC CDSSTISFIT APVAPEITSV
660 670 680 690 700
EYFNSLLYIS WTYGDATTDL SHSRMLHWMV VAEGRKKIKK SVTRNVMTAI
710 720 730 740 750
LSLPPGDIYN LSVTACTERG SNTSLPRLVK LEPAPPKSLF AVNKTQTSVT
760 770 780 790 800
LLWVEEGVAD FFEVFCQQLG SGHNGKLQEP VAVSSHVVTI SSLLPATAYN
810 820 830 840 850
CSVTSFSHDT PSVPTFIAVS TMVTEVNPNV VVISVLAILS TLLIGLLLVT
860 870 880 890 900
LVILRKKHLQ MARECGAGTF VNFASLEREG KLPYSWRRSV FALLTLLPSC
910 920 930 940 950
LWTDYLLAFY INPWSKNGLK KRKLTNPVQL DDFDSYIKDM AKDSDYKFSL
960 970 980 990 1000
QFEELKLIGL DIPHFAADLP LNRCKNRYTN ILPYDFSRVR LVSMNEEEGA
1010 1020 1030 1040 1050
DYINANYIPG YNSPQEYIAT QGPLPETRND FWKMVLQQKS HIIVMLTQCN
1060 1070 1080 1090 1100
EKRRVKCDHY WPFTEEPIAY GDITVEMVSE EEEEDWASRH FRINYADEAQ
1110 1120 1130 1140 1150
DVMHFNYTAW PDHGVPPANA AESILQFVFT VRQQAAKSKG PMIIHCSAGV
1160 1170 1180 1190 1200
GRTGTFIALD RLLQHIRDHE FVDILGLVSE MRSYRMSMVQ TEEQYIFIHQ
1210 1220
CVQLMWLRKK QQFCISDVIY ENVSKS
Length:1,226
Mass (Da):138,589
Last modified:April 5, 2011 - v1
Checksum:i3FF0BE298354BC32
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1184Y → H in AAH52743 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC093120 Genomic DNA No translation available.
AC144767 Genomic DNA No translation available.
AC163627 Genomic DNA No translation available.
BC052743 mRNA Translation: AAH52743.1
CCDSiCCDS20664.1
RefSeqiNP_035346.3, NM_011216.3
UniGeneiMm.186361
Mm.491536

Genome annotation databases

EnsembliENSMUST00000077115; ENSMUSP00000076364; ENSMUSG00000030223
GeneIDi19277
KEGGimmu:19277
UCSCiuc009emv.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPTPRO_MOUSE
AccessioniPrimary (citable) accession number: E9Q612
Secondary accession number(s): Q7TSY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: April 5, 2011
Last modified: February 28, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome