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E9Q612

- PTPRO_MOUSE

UniProt

E9Q612 - PTPRO_MOUSE

Protein

Receptor-type tyrosine-protein phosphatase O

Gene

Ptpro

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (05 Apr 2011)
      Previous versions | rss
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    Functioni

    Possesses tyrosine phosphatase activity. Plays a role in regulating the glomerular pressure/filtration rate relationship through an effect on podocyte structure and function.1 Publication

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei1112 – 11121SubstrateBy similarity
    Active sitei1146 – 11461Phosphocysteine intermediatePROSITE-ProRule annotation
    Binding sitei1190 – 11901SubstrateBy similarity

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. protein homodimerization activity Source: UniProtKB
    3. protein tyrosine phosphatase activity Source: UniProtKB
    4. Wnt-protein binding Source: UniProtKB

    GO - Biological processi

    1. axon guidance Source: UniProtKB
    2. cell morphogenesis Source: UniProtKB
    3. glomerular visceral epithelial cell differentiation Source: UniProtKB
    4. glomerulus development Source: UniProtKB
    5. lamellipodium assembly Source: UniProtKB
    6. monocyte chemotaxis Source: UniProtKB
    7. negative regulation of canonical Wnt signaling pathway Source: UniProtKB
    8. negative regulation of glomerular filtration Source: UniProtKB
    9. peptidyl-tyrosine dephosphorylation Source: UniProtKB
    10. regulation of glomerular filtration Source: UniProtKB
    11. slit diaphragm assembly Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase, Receptor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Receptor-type tyrosine-protein phosphatase O (EC:3.1.3.48)
    Short name:
    R-PTP-O
    Alternative name(s):
    Glomerular epithelial protein 1
    Protein tyrosine phosphatase U2
    Short name:
    PTP-U2
    Short name:
    PTPase U2
    Gene namesi
    Name:Ptpro
    Synonyms:GLEPP1, Ptpn15, PTPU2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1097152. Ptpro.

    Subcellular locationi

    Membrane By similarity; Single-pass type I membrane protein By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB
    2. dendritic spine Source: UniProtKB
    3. integral component of membrane Source: UniProtKB-KW
    4. lateral plasma membrane Source: UniProtKB
    5. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Modification of podocyte structure such that the normal octopoid podocyte is simplified to a more amoeboid structure and that the foot processes are shorter and broader than normal. These changes are associated with altered distribution of the podocyte intermediate cytoskeletal protein vimentin/VIM. Mutant animals have a reduced glomerular filtration rate and reduced glomerular nephrin (NPHS1) content. However, there is no evidence of proteinuria. After removal of one or more kidneys, Ptpro-null animals have higher blood pressure than does their wild-type littermates.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 12261197Receptor-type tyrosine-protein phosphatase OPRO_0000414059Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi227 – 2271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi471 – 4711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi500 – 5001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi710 – 7101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi743 – 7431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi800 – 8001N-linked (GlcNAc...)Sequence Analysis
    Modified residuei875 – 8751Phosphoserine1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiE9Q612.

    PTM databases

    PhosphoSiteiE9Q612.

    Expressioni

    Gene expression databases

    BgeeiE9Q612.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Wnt3aP274672EBI-8183885,EBI-2899665

    Protein-protein interaction databases

    IntActiE9Q612. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliE9Q612.
    SMRiE9Q612. Positions 926-1218.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini30 – 832803ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini854 – 1226373CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei833 – 85321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 11586Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini339 – 43597Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini445 – 54197Fibronectin type-III 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini542 – 63897Fibronectin type-III 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini641 – 73494Fibronectin type-III 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini735 – 82793Fibronectin type-III 6PROSITE-ProRule annotationAdd
    BLAST
    Domaini948 – 1205258Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1146 – 11527Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 6 fibronectin type-III domains.PROSITE-ProRule annotation
    Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00750000117631.
    HOGENOMiHOG000115792.
    HOVERGENiHBG053763.
    InParanoidiQ7TSY7.
    KOiK18035.
    OMAiSTMVTEM.
    OrthoDBiEOG7PCJFZ.
    TreeFamiTF351926.

    Family and domain databases

    Gene3Di2.60.40.10. 3 hits.
    3.90.190.10. 1 hit.
    InterProiIPR003961. Fibronectin_type3.
    IPR013783. Ig-like_fold.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view]
    PfamiPF00041. fn3. 2 hits.
    PF00102. Y_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00700. PRTYPHPHTASE.
    SMARTiSM00060. FN3. 4 hits.
    SM00194. PTPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 3 hits.
    SSF52799. SSF52799. 1 hit.
    PROSITEiPS50853. FN3. 5 hits.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    E9Q612-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGHLPRGTLG GRRLLPLLGL FVLLKIVTTF HVAVQDDNNI VVSLEASDIV     50
    SPASVYVVRV AGESKNYFFE FEEFNSTLPP PVVFKATYHG LYYIITLVVV 100
    NGNVVTKPSR SITVLTKPLP VTSVSIYDYK PSPETGVLFE IHYPEKYNVF 150
    SRVNISYWEG RDFRTMLYKD FFKGKTVFNH WLPGLCYSNI TFQLVSEATF 200
    NKSTLVEYSG VSHEPKQHRT APYPPRNISV RFVNLNKNNW EEPSGSFPED 250
    SFIKPPQDSI GRDRRFHFPE ETPETPPSNV SSGSPPSNVS SAWPDPNSTD 300
    YESTSQPFWW DSASAAPENE EDFVSALPAD YDTETTLDRT EKPTADPFSA 350
    FPVQMTLSWL PPKPPTAFDG FNILIEREEN FTDYLTVDEE AHEFVAELKE 400
    PGKYKLSVTT FSSSGACETR KSQSAKSLSF YISPTGEWIE ELTEKPQHVS 450
    VHVLSSTTAL MSWTSSQENY NSTIVSVVSL TCQKQKESQR LEKQYCTQVN 500
    SSKPVIENLV PGAQYQVVMY LRKGPLIGPP SDPVTFAIVP TGIKDLMLYP 550
    LGPTAVVLSW TRPILGVFRK YVVEMFYFNP TTMTSEWTTY YEIAATVSLT 600
    ASVRIASLLP AWYYNFRVTM VTWGDPELSC CDSSTISFIT APVAPEITSV 650
    EYFNSLLYIS WTYGDATTDL SHSRMLHWMV VAEGRKKIKK SVTRNVMTAI 700
    LSLPPGDIYN LSVTACTERG SNTSLPRLVK LEPAPPKSLF AVNKTQTSVT 750
    LLWVEEGVAD FFEVFCQQLG SGHNGKLQEP VAVSSHVVTI SSLLPATAYN 800
    CSVTSFSHDT PSVPTFIAVS TMVTEVNPNV VVISVLAILS TLLIGLLLVT 850
    LVILRKKHLQ MARECGAGTF VNFASLEREG KLPYSWRRSV FALLTLLPSC 900
    LWTDYLLAFY INPWSKNGLK KRKLTNPVQL DDFDSYIKDM AKDSDYKFSL 950
    QFEELKLIGL DIPHFAADLP LNRCKNRYTN ILPYDFSRVR LVSMNEEEGA 1000
    DYINANYIPG YNSPQEYIAT QGPLPETRND FWKMVLQQKS HIIVMLTQCN 1050
    EKRRVKCDHY WPFTEEPIAY GDITVEMVSE EEEEDWASRH FRINYADEAQ 1100
    DVMHFNYTAW PDHGVPPANA AESILQFVFT VRQQAAKSKG PMIIHCSAGV 1150
    GRTGTFIALD RLLQHIRDHE FVDILGLVSE MRSYRMSMVQ TEEQYIFIHQ 1200
    CVQLMWLRKK QQFCISDVIY ENVSKS 1226
    Length:1,226
    Mass (Da):138,589
    Last modified:April 5, 2011 - v1
    Checksum:i3FF0BE298354BC32
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1184 – 11841Y → H in AAH52743. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC093120 Genomic DNA. No translation available.
    AC144767 Genomic DNA. No translation available.
    AC163627 Genomic DNA. No translation available.
    BC052743 mRNA. Translation: AAH52743.1.
    CCDSiCCDS20664.1.
    RefSeqiNP_035346.3. NM_011216.3.
    UniGeneiMm.186361.
    Mm.491536.

    Genome annotation databases

    EnsembliENSMUST00000077115; ENSMUSP00000076364; ENSMUSG00000030223.
    GeneIDi19277.
    KEGGimmu:19277.
    UCSCiuc009emv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC093120 Genomic DNA. No translation available.
    AC144767 Genomic DNA. No translation available.
    AC163627 Genomic DNA. No translation available.
    BC052743 mRNA. Translation: AAH52743.1 .
    CCDSi CCDS20664.1.
    RefSeqi NP_035346.3. NM_011216.3.
    UniGenei Mm.186361.
    Mm.491536.

    3D structure databases

    ProteinModelPortali E9Q612.
    SMRi E9Q612. Positions 926-1218.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi E9Q612. 1 interaction.

    PTM databases

    PhosphoSitei E9Q612.

    Proteomic databases

    PRIDEi E9Q612.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000077115 ; ENSMUSP00000076364 ; ENSMUSG00000030223 .
    GeneIDi 19277.
    KEGGi mmu:19277.
    UCSCi uc009emv.2. mouse.

    Organism-specific databases

    CTDi 5800.
    MGIi MGI:1097152. Ptpro.

    Phylogenomic databases

    GeneTreei ENSGT00750000117631.
    HOGENOMi HOG000115792.
    HOVERGENi HBG053763.
    InParanoidi Q7TSY7.
    KOi K18035.
    OMAi STMVTEM.
    OrthoDBi EOG7PCJFZ.
    TreeFami TF351926.

    Miscellaneous databases

    NextBioi 296184.
    PROi E9Q612.
    SOURCEi Search...

    Gene expression databases

    Bgeei E9Q612.

    Family and domain databases

    Gene3Di 2.60.40.10. 3 hits.
    3.90.190.10. 1 hit.
    InterProi IPR003961. Fibronectin_type3.
    IPR013783. Ig-like_fold.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view ]
    Pfami PF00041. fn3. 2 hits.
    PF00102. Y_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00700. PRTYPHPHTASE.
    SMARTi SM00060. FN3. 4 hits.
    SM00194. PTPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 3 hits.
    SSF52799. SSF52799. 1 hit.
    PROSITEi PS50853. FN3. 5 hits.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "Altered podocyte structure in GLEPP1 (Ptpro)-deficient mice associated with hypertension and low glomerular filtration rate."
      Wharram B.L., Goyal M., Gillespie P.J., Wiggins J.E., Kershaw D.B., Holzman L.B., Dysko R.C., Saunders T.L., Samuelson L.C., Wiggins R.C.
      J. Clin. Invest. 106:1281-1290(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-875, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPTPRO_MOUSE
    AccessioniPrimary (citable) accession number: E9Q612
    Secondary accession number(s): Q7TSY7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 14, 2011
    Last sequence update: April 5, 2011
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3