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E9Q5G3

- KIF23_MOUSE

UniProt

E9Q5G3 - KIF23_MOUSE

Protein

Kinesin-like protein KIF23

Gene

Kif23

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 32 (01 Oct 2014)
      Sequence version 1 (05 Apr 2011)
      Previous versions | rss
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    Functioni

    Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi112 – 1198ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. microtubule motor activity Source: InterPro

    GO - Biological processi

    1. cytokinesis Source: Ensembl
    2. microtubule-based movement Source: InterPro
    3. positive regulation of cytokinesis Source: Ensembl
    4. spindle midzone assembly involved in mitosis Source: Ensembl

    Keywords - Molecular functioni

    Motor protein

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196550. MHC class II antigen presentation.
    REACT_199117. Kinesins.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinesin-like protein KIF23
    Gene namesi
    Name:Kif23
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1919069. Kif23.

    Subcellular locationi

    Nucleus By similarity. Cytoplasmcytoskeletonspindle By similarity. Midbody 1 Publication
    Note: Localizes to the interzone of mitotic spindles By similarity. Detected at the midbody during later stages of mitotic cytokinesis.By similarity

    GO - Cellular componenti

    1. centralspindlin complex Source: Ensembl
    2. centrosome Source: Ensembl
    3. cytoplasm Source: UniProtKB-KW
    4. intercellular bridge Source: MGI
    5. kinesin complex Source: InterPro
    6. microtubule Source: UniProtKB-KW
    7. midbody Source: UniProtKB-SubCell
    8. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953Kinesin-like protein KIF23PRO_0000425085Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei155 – 1551PhosphoserineBy similarity
    Modified residuei160 – 1601PhosphoserineBy similarity
    Modified residuei606 – 6061PhosphoserineBy similarity
    Modified residuei739 – 7391PhosphothreonineBy similarity
    Modified residuei807 – 8071PhosphoserineBy similarity
    Modified residuei860 – 8601Phosphoserine1 Publication

    Post-translational modificationi

    Ubiquitinated. Deubiquitinated by USP8/UBPY By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiE9Q5G3.
    PRIDEiE9Q5G3.

    Expressioni

    Tissue specificityi

    Detected in testis and ovary from newborn mice (at protein level). Detected in brain, spinal cord and small intestine.2 Publications

    Gene expression databases

    ArrayExpressiE9Q5G3.
    BgeeiE9Q5G3.

    Interactioni

    Subunit structurei

    Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Interacts with RACGAP1; the interaction is direct. Interacts with ECT2 and PRC1. Interacts with ANXA11 during cytokinesis. Interacts with BIRC6/bruce and USP8/UBPY By similarity. Interacts with ARF6, forming heterodimers and heterotetramers.By similarity1 Publication

    Protein-protein interaction databases

    IntActiE9Q5G3. 12 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliE9Q5G3.
    SMRiE9Q5G3. Positions 21-436, 811-896.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 436412Kinesin motorPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili542 – 61877Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi7 – 115Nuclear localization signalSequence Analysis

    Sequence similaritiesi

    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation
    Contains 1 kinesin motor domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    GeneTreeiENSGT00530000063172.
    KOiK17387.
    OMAiQTSLKDP.
    TreeFamiTF105232.

    Family and domain databases

    Gene3Di3.40.850.10. 2 hits.
    InterProiIPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR24115. PTHR24115. 1 hit.
    PfamiPF00225. Kinesin. 1 hit.
    [Graphical view]
    PRINTSiPR00380. KINESINHEAVY.
    SMARTiSM00129. KISc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.
    PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E9Q5G3-1 [UniParc]FASTAAdd to Basket

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    MKSAKAKTVR KPVIKKGSQT NLKDPVGVYC RVRPLSFPDQ ECCVEVINST    50
    TLQLHTPEGY RLNRNGDYKE TQYSFKRVFG THTTQKELFD VVANPLVDDL 100
    IHGKNGLLFT YGVTGSGKTY TMTGSPGSGG LLPRCLNMIF NSIGSFQAKR 150
    YVFKSNDRNS MEIQCEVDAL LERQKREALP IPKTPSSKRQ ADPEFADMIN 200
    VQEFCKAEEV DEDSVYGVFV SYIEIYNNYI YDLLEEVQFD PIKPKLPQSK 250
    TLREDKNHNM YVAGCTEVEV KSTEEAFEVF WRGQKKRRIA NTHLNRESSR 300
    SHSVFSIKLV QAPLDADGDN VLQEKEQITI SQLSLVDLAG SERTNRTKAE 350
    GNRLREAGNI NQSLMTLRTC MEVLRENQTY GTNKMVPYRD SKLTHLFKNY 400
    FDGEGKVRMI VCVNPKAEDY EESLQVMRFA EVTQEVEVAR PVDKVICGLT 450
    PGRRYRNLPR GGPVGDEPLV PEVILQSFPP LPPCKLLDIN DEETLPKLAD 500
    TLEKRHHLRQ LMTEDLNKKC LAFKALLKEF DNSLSNKENY VQEKLNEREK 550
    VISGQKLEIE RLEKKNKTLE YKIEILEKTT TIYEEDKRNL QQELESQNQK 600
    LQRQFSDKRR LEARLQGMVT ETSMKWQKEC ERRVAATQLE MQNKLWVKDE 650
    KLKQLKAIVT EPKPEKPERP SRERDREKII PRSVSPSPLP LSSNNIAQIS 700
    NGQQLMSQPQ LHRRSNSCSS ISVASCISEW EQKLSPFSTP VNVTSLARHR 750
    QQEPGQSKTC IVSDRRRGMC WTEGREMVPT FSSEIGVEED HCRRNTPIPV 800
    RHRRSRSAGS RWVDHKPASN VQTETVMQPH VPHAITVSVA NEKALAKCEK 850
    YMLTHQELAS DGEIQTKVIK GDVYKTRGGG QSVQFTDIET LKQELPTGSR 900
    KRRSSTLAPA QPDGTESEWT DVETRCSVAV EMRAGSQLGP GYQHHAQPKR 950
    KKP 953
    Length:953
    Mass (Da):108,776
    Last modified:April 5, 2011 - v1
    Checksum:iDE6E050D59A18513
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti464 – 4641V → I in AAH47273. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC151969 Genomic DNA. No translation available.
    BC047273 mRNA. Translation: AAH47273.1.
    AB054027 Genomic DNA. Translation: BAB32491.1.
    CCDSiCCDS40662.1.
    RefSeqiNP_077207.3. NM_024245.4.
    UniGeneiMm.259374.

    Genome annotation databases

    EnsembliENSMUST00000034815; ENSMUSP00000034815; ENSMUSG00000032254.
    GeneIDi71819.
    KEGGimmu:71819.
    UCSCiuc009pzu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC151969 Genomic DNA. No translation available.
    BC047273 mRNA. Translation: AAH47273.1 .
    AB054027 Genomic DNA. Translation: BAB32491.1 .
    CCDSi CCDS40662.1.
    RefSeqi NP_077207.3. NM_024245.4.
    UniGenei Mm.259374.

    3D structure databases

    ProteinModelPortali E9Q5G3.
    SMRi E9Q5G3. Positions 21-436, 811-896.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi E9Q5G3. 12 interactions.

    Proteomic databases

    MaxQBi E9Q5G3.
    PRIDEi E9Q5G3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034815 ; ENSMUSP00000034815 ; ENSMUSG00000032254 .
    GeneIDi 71819.
    KEGGi mmu:71819.
    UCSCi uc009pzu.2. mouse.

    Organism-specific databases

    CTDi 9493.
    MGIi MGI:1919069. Kif23.

    Phylogenomic databases

    GeneTreei ENSGT00530000063172.
    KOi K17387.
    OMAi QTSLKDP.
    TreeFami TF105232.

    Enzyme and pathway databases

    Reactomei REACT_196550. MHC class II antigen presentation.
    REACT_199117. Kinesins.

    Miscellaneous databases

    ChiTaRSi KIF23. mouse.
    NextBioi 334596.
    PROi E9Q5G3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi E9Q5G3.
    Bgeei E9Q5G3.

    Family and domain databases

    Gene3Di 3.40.850.10. 2 hits.
    InterProi IPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR24115. PTHR24115. 1 hit.
    Pfami PF00225. Kinesin. 1 hit.
    [Graphical view ]
    PRINTSi PR00380. KINESINHEAVY.
    SMARTi SM00129. KISc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N-3.
      Tissue: Mammary tumor.
    3. "All kinesin superfamily protein, KIF, genes in mouse and human."
      Miki H., Setou M., Kaneshiro K.
      Proc. Natl. Acad. Sci. U.S.A. 98:7004-7011(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 203-340, TISSUE SPECIFICITY.
    4. "Mouse TEX14 is required for embryonic germ cell intercellular bridges but not female fertility."
      Greenbaum M.P., Iwamori N., Agno J.E., Matzuk M.M.
      Biol. Reprod. 80:449-457(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    5. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-860, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis."
      Makyio H., Ohgi M., Takei T., Takahashi S., Takatsu H., Katoh Y., Hanai A., Ueda T., Kanaho Y., Xie Y., Shin H.W., Kamikubo H., Kataoka M., Kawasaki M., Kato R., Wakatsuki S., Nakayama K.
      EMBO J. 31:2590-2603(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ARF6.

    Entry informationi

    Entry nameiKIF23_MOUSE
    AccessioniPrimary (citable) accession number: E9Q5G3
    Secondary accession number(s): Q80V30, Q99PT8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 22, 2014
    Last sequence update: April 5, 2011
    Last modified: October 1, 2014
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3