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E9Q5G3 (KIF23_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kinesin-like protein KIF23
Gene names
Name:Kif23
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length953 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro By similarity.

Subunit structure

Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Interacts with RACGAP1; the interaction is direct. Interacts with ECT2 and PRC1. Interacts with ANXA11 during cytokinesis. Interacts with BIRC6/bruce and USP8/UBPY By similarity. Interacts with ARF6, forming heterodimers and heterotetramers. Ref.6

Subcellular location

Nucleus By similarity. Cytoplasmcytoskeletonspindle By similarity. Midbody. Note: Localizes to the interzone of mitotic spindles By similarity. Detected at the midbody during later stages of mitotic cytokinesis. Ref.4

Tissue specificity

Detected in testis and ovary from newborn mice (at protein level). Detected in brain, spinal cord and small intestine. Ref.3 Ref.4

Post-translational modification

Ubiquitinated. Deubiquitinated by USP8/UBPY By similarity.

Sequence similarities

Belongs to the kinesin-like protein family.

Contains 1 kinesin-motor domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 953953Kinesin-like protein KIF23
PRO_0000425085

Regions

Domain22 – 362341Kinesin-motor
Nucleotide binding112 – 1198ATP Potential
Coiled coil542 – 61877 Potential
Motif7 – 115Nuclear localization signal Potential

Amino acid modifications

Modified residue1551Phosphoserine By similarity
Modified residue1601Phosphoserine By similarity
Modified residue6061Phosphoserine By similarity
Modified residue7391Phosphothreonine By similarity
Modified residue8071Phosphoserine By similarity
Modified residue8601Phosphoserine Ref.5

Experimental info

Sequence conflict4641V → I in AAH47273. Ref.2

Sequences

Sequence LengthMass (Da)Tools
E9Q5G3 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: DE6E050D59A18513

FASTA953108,776
        10         20         30         40         50         60 
MKSAKAKTVR KPVIKKGSQT NLKDPVGVYC RVRPLSFPDQ ECCVEVINST TLQLHTPEGY 

        70         80         90        100        110        120 
RLNRNGDYKE TQYSFKRVFG THTTQKELFD VVANPLVDDL IHGKNGLLFT YGVTGSGKTY 

       130        140        150        160        170        180 
TMTGSPGSGG LLPRCLNMIF NSIGSFQAKR YVFKSNDRNS MEIQCEVDAL LERQKREALP 

       190        200        210        220        230        240 
IPKTPSSKRQ ADPEFADMIN VQEFCKAEEV DEDSVYGVFV SYIEIYNNYI YDLLEEVQFD 

       250        260        270        280        290        300 
PIKPKLPQSK TLREDKNHNM YVAGCTEVEV KSTEEAFEVF WRGQKKRRIA NTHLNRESSR 

       310        320        330        340        350        360 
SHSVFSIKLV QAPLDADGDN VLQEKEQITI SQLSLVDLAG SERTNRTKAE GNRLREAGNI 

       370        380        390        400        410        420 
NQSLMTLRTC MEVLRENQTY GTNKMVPYRD SKLTHLFKNY FDGEGKVRMI VCVNPKAEDY 

       430        440        450        460        470        480 
EESLQVMRFA EVTQEVEVAR PVDKVICGLT PGRRYRNLPR GGPVGDEPLV PEVILQSFPP 

       490        500        510        520        530        540 
LPPCKLLDIN DEETLPKLAD TLEKRHHLRQ LMTEDLNKKC LAFKALLKEF DNSLSNKENY 

       550        560        570        580        590        600 
VQEKLNEREK VISGQKLEIE RLEKKNKTLE YKIEILEKTT TIYEEDKRNL QQELESQNQK 

       610        620        630        640        650        660 
LQRQFSDKRR LEARLQGMVT ETSMKWQKEC ERRVAATQLE MQNKLWVKDE KLKQLKAIVT 

       670        680        690        700        710        720 
EPKPEKPERP SRERDREKII PRSVSPSPLP LSSNNIAQIS NGQQLMSQPQ LHRRSNSCSS 

       730        740        750        760        770        780 
ISVASCISEW EQKLSPFSTP VNVTSLARHR QQEPGQSKTC IVSDRRRGMC WTEGREMVPT 

       790        800        810        820        830        840 
FSSEIGVEED HCRRNTPIPV RHRRSRSAGS RWVDHKPASN VQTETVMQPH VPHAITVSVA 

       850        860        870        880        890        900 
NEKALAKCEK YMLTHQELAS DGEIQTKVIK GDVYKTRGGG QSVQFTDIET LKQELPTGSR 

       910        920        930        940        950 
KRRSSTLAPA QPDGTESEWT DVETRCSVAV EMRAGSQLGP GYQHHAQPKR KKP 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N-3.
Tissue: Mammary tumor.
[3]"All kinesin superfamily protein, KIF, genes in mouse and human."
Miki H., Setou M., Kaneshiro K.
Proc. Natl. Acad. Sci. U.S.A. 98:7004-7011(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 203-340, TISSUE SPECIFICITY.
[4]"Mouse TEX14 is required for embryonic germ cell intercellular bridges but not female fertility."
Greenbaum M.P., Iwamori N., Agno J.E., Matzuk M.M.
Biol. Reprod. 80:449-457(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-860, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis."
Makyio H., Ohgi M., Takei T., Takahashi S., Takatsu H., Katoh Y., Hanai A., Ueda T., Kanaho Y., Xie Y., Shin H.W., Kamikubo H., Kataoka M., Kawasaki M., Kato R., Wakatsuki S., Nakayama K.
EMBO J. 31:2590-2603(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARF6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC151969 Genomic DNA. No translation available.
BC047273 mRNA. Translation: AAH47273.1.
AB054027 Genomic DNA. Translation: BAB32491.1.
RefSeqNP_077207.3. NM_024245.4.
UniGeneMm.259374.

3D structure databases

ProteinModelPortalE9Q5G3.
SMRE9Q5G3. Positions 21-444, 811-896.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActE9Q5G3. 12 interactions.

Proteomic databases

PRIDEE9Q5G3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034815; ENSMUSP00000034815; ENSMUSG00000032254.
GeneID71819.
KEGGmmu:71819.
UCSCuc009pzu.2. mouse.

Organism-specific databases

CTD9493.
MGIMGI:1919069. Kif23.

Phylogenomic databases

GeneTreeENSGT00530000063172.
KOK17387.
OMAQTSLKDP.
TreeFamTF105232.

Gene expression databases

ArrayExpressE9Q5G3.
BgeeE9Q5G3.

Family and domain databases

Gene3D3.40.850.10. 2 hits.
InterProIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR24115. PTHR24115. 1 hit.
PfamPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSPR00380. KINESINHEAVY.
SMARTSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
PROSITEPS00411. KINESIN_MOTOR_DOMAIN1. 1 hit.
PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSKIF23. mouse.
NextBio334596.
PROE9Q5G3.
SOURCESearch...

Entry information

Entry nameKIF23_MOUSE
AccessionPrimary (citable) accession number: E9Q5G3
Secondary accession number(s): Q80V30, Q99PT8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: April 5, 2011
Last modified: April 16, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot