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Protein

Nucleolar and coiled-body phosphoprotein 1

Gene

Nolc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (By similarity). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (By similarity). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:11424213). It has intrinsic GTPase and ATPase activities (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • nucleolus organization Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar and coiled-body phosphoprotein 1Curated
Alternative name(s):
140 kDa nucleolar phosphoprotein1 Publication
Short name:
Nopp1401 Publication
Gene namesi
Name:Nolc1Imported
Synonyms:Kiaa00351 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1918019. Nolc1.

Subcellular locationi

  • Nucleusnucleolus By similarity
  • Cytoplasm By similarity

  • Note: Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase.By similarity

GO - Cellular componenti

  • Cajal body Source: MGI
  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • small nuclear ribonucleoprotein complex Source: MGI

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004396411 – 702Nucleolar and coiled-body phosphoprotein 1Add BLAST702

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33N6-acetyllysineBy similarity1
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei88PhosphoserineBy similarity1
Modified residuei92PhosphoserineBy similarity1
Cross-linki201Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei371PhosphoserineBy similarity1
Modified residuei372PhosphoserineBy similarity1
Modified residuei375PhosphoserineBy similarity1
Modified residuei421N6-acetyllysine; alternateBy similarity1
Cross-linki421Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Modified residuei563PhosphoserineBy similarity1
Cross-linki575Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei585PhosphoserineBy similarity1
Cross-linki607Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei610PhosphothreonineBy similarity1
Modified residuei613PhosphothreonineBy similarity1
Modified residuei625PhosphoserineBy similarity1
Modified residuei646PhosphoserineBy similarity1
Modified residuei666N6-acetyllysineBy similarity1
Modified residuei686Omega-N-methylarginineBy similarity1
Modified residuei689PhosphoserineBy similarity1
Modified residuei701PhosphoserineBy similarity1

Post-translational modificationi

Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. NOLC1 is one of the mostly phosphorylated proteins in the cell.By similarity
Ubiquitinated. Monoubiquitination by the BCR(KBTBD8) complex promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiE9Q5C9.
PaxDbiE9Q5C9.
PeptideAtlasiE9Q5C9.
PRIDEiE9Q5C9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000015176.
GenevisibleiE9Q5C9. MM.

Interactioni

Subunit structurei

Interacts with RNA polymerase I 194 kDa subunit (RPA194) and with casein kinase-II (By similarity). Interacts with DKC1/NAP57, NOP58 and fibrillarin (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-1854239.
STRINGi10090.ENSMUSP00000128331.

Structurei

3D structure databases

ProteinModelPortaliE9Q5C9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 42LisHPROSITE-ProRule annotationAdd BLAST33
Repeati85 – 96Acidic serine cluster 1By similarityAdd BLAST12
Repeati130 – 141Acidic serine cluster 2By similarityAdd BLAST12
Repeati176 – 187Acidic serine cluster 3By similarityAdd BLAST12
Repeati232 – 241Acidic serine cluster 4By similarity10
Repeati273 – 284Acidic serine cluster 5By similarityAdd BLAST12
Repeati334 – 345Acidic serine cluster 6By similarityAdd BLAST12
Repeati372 – 383Acidic serine cluster 7By similarityAdd BLAST12
Repeati431 – 442Acidic serine cluster 8By similarityAdd BLAST12
Repeati474 – 484Acidic serine cluster 9By similarityAdd BLAST11
Repeati521 – 531Acidic serine cluster 10By similarityAdd BLAST11
Repeati555 – 566Acidic serine cluster 11By similarityAdd BLAST12

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 56611 X 12 AA approximate repeats of an acidic serine clusterBy similarityAdd BLAST482
Regioni215 – 390Interaction with RPA194By similarityAdd BLAST176

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi69 – 83Nuclear localization signalSequence analysisAdd BLAST15
Motifi392 – 590Nuclear localization signalSequence analysisAdd BLAST199
Motifi604 – 620Nuclear localization signalSequence analysisAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi330 – 528Ser-richPROSITE-ProRule annotationAdd BLAST199

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2992. Eukaryota.
ENOG4111U5P. LUCA.
GeneTreeiENSGT00730000111092.
HOGENOMiHOG000113823.
HOVERGENiHBG075370.
InParanoidiE9Q5C9.
OMAiKKKAGPY.
OrthoDBiEOG091G127U.
TreeFamiTF341730.

Family and domain databases

InterProiView protein in InterPro
IPR006594. LisH.
IPR007718. SRP40_C.
PfamiView protein in Pfam
PF05022. SRP40_C. 1 hit.
PROSITEiView protein in PROSITE
PS50896. LISH. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: E9Q5C9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADTGLRRVV PSDLYPLVLR FLRDSQLSEV ASKFAKATGA TQQDANASSL
60 70 80 90 100
LDIYSFWLNR STKAPKVKLQ SNGPVTKKAK KETSSSDSSE DSSEDEDKKA
110 120 130 140 150
QGLPTQKAAA QVKRASVPQH AGKAAAKASE SSSSEESSEE EEEDKKKKPV
160 170 180 190 200
QQKAAKPQAK AVRPPAKKAE SSESDSDSDS DSSSEEETPQ TQKPKAAVAA
210 220 230 240 250
KAQTKAEAKP GTPAKAQPKV ANGKAAASSS SSSSSDDSEE EKKAAAPPKK
260 270 280 290 300
TVPKKQVVAK APVKVAAAPT QKSSSSEDSS SEEEEGQRQP MKKKAGPYSS
310 320 330 340 350
VPPPSVPLPK KSPGTQAPKK AAAQTQPADS SDDSSDDSDS SSEEEKKPPA
360 370 380 390 400
KTVVSKTPAK AAPVKKKAES SSDSSDSDSS EDEAPAKPVS TTKSPKPAVT
410 420 430 440 450
PKPSAAKAVT TPKQPAGSNQ KPQSRKADSS SSEEESSSSE EEEASKKSAT
460 470 480 490 500
TPKAKVTAKA APAKQAPQAA GDSSSDSDSS SSEEEEKTPK PPAKKKAAGG
510 520 530 540 550
AVSTPAPGKK AEAKSSSSSS SSSSEDSSEE EKKKKPKATT PKIQASKANG
560 570 580 590 600
TPASLNGKAA KESEEEEEEE ETEEKKKAAG TKPGSGKKRK QNETADEATT
610 620 630 640 650
PQAKKVKLET PNTFPKRKKG ERRASSPFRR VREEEIEVDS RVADNSFDAK
660 670 680 690 700
RGAAGDWGER ANQVLKFTKG KSFRHEKTKK KRGSYRGGSI SVQVNSVKFD

SE
Length:702
Mass (Da):73,698
Last modified:April 5, 2011 - v1
Checksum:i08DF638A34B50ABD
GO
Isoform 2 (identifier: E9Q5C9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     657-664: WGERANQV → GGSEPIRF
     665-702: Missing.

Show »
Length:664
Mass (Da):69,273
Checksum:iC2F5F7F05453307C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59 – 60NR → K in BAC26327 (PubMed:16141072).Curated2
Sequence conflicti59 – 60NR → K in BAE27690 (PubMed:16141072).Curated2
Sequence conflicti59 – 60NR → K in BAE31612 (PubMed:16141072).Curated2
Sequence conflicti59 – 60NR → K in BAE29474 (PubMed:16141072).Curated2
Sequence conflicti59 – 60NR → K in BAE30048 (PubMed:16141072).Curated2
Sequence conflicti151Missing in BAE38994 (PubMed:16141072).Curated1
Sequence conflicti151Missing in BAC26327 (PubMed:16141072).Curated1
Sequence conflicti151Missing in BAE31612 (PubMed:16141072).Curated1
Sequence conflicti151Missing in BAE29474 (PubMed:16141072).Curated1
Sequence conflicti151Missing in BAE30048 (PubMed:16141072).Curated1
Sequence conflicti227A → ASSS in BAC97850 (PubMed:14621295).Curated1
Sequence conflicti489P → T in BAE31612 (PubMed:16141072).Curated1
Sequence conflicti489P → T in BAE29474 (PubMed:16141072).Curated1
Sequence conflicti489P → T in BAE30048 (PubMed:16141072).Curated1
Sequence conflicti514 – 515KS → E in BAC97850 (PubMed:14621295).Curated2
Sequence conflicti657W → R in BAE31612 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058892657 – 664WGERANQV → GGSEPIRF in isoform 2. 8
Alternative sequenceiVSP_058893665 – 702Missing in isoform 2. Add BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029150 mRNA. Translation: BAC26327.1.
AK147118 mRNA. Translation: BAE27690.1.
AK150332 mRNA. Translation: BAE29474.1.
AK151031 mRNA. Translation: BAE30048.1.
AK152941 mRNA. Translation: BAE31612.1.
AK166753 mRNA. Translation: BAE38994.1.
AC140233 Genomic DNA. No translation available.
AK129040 mRNA. Translation: BAC97850.1.
RefSeqiNP_001034441.1. NM_001039352.2.
UniGeneiMm.402190.
Mm.486316.

Genome annotation databases

EnsembliENSMUST00000165017; ENSMUSP00000128331; ENSMUSG00000015176.
GeneIDi70769.
UCSCiuc008hsh.3. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029150 mRNA. Translation: BAC26327.1.
AK147118 mRNA. Translation: BAE27690.1.
AK150332 mRNA. Translation: BAE29474.1.
AK151031 mRNA. Translation: BAE30048.1.
AK152941 mRNA. Translation: BAE31612.1.
AK166753 mRNA. Translation: BAE38994.1.
AC140233 Genomic DNA. No translation available.
AK129040 mRNA. Translation: BAC97850.1.
RefSeqiNP_001034441.1. NM_001039352.2.
UniGeneiMm.402190.
Mm.486316.

3D structure databases

ProteinModelPortaliE9Q5C9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1854239.
STRINGi10090.ENSMUSP00000128331.

Proteomic databases

MaxQBiE9Q5C9.
PaxDbiE9Q5C9.
PeptideAtlasiE9Q5C9.
PRIDEiE9Q5C9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000165017; ENSMUSP00000128331; ENSMUSG00000015176.
GeneIDi70769.
UCSCiuc008hsh.3. mouse.

Organism-specific databases

CTDi9221.
MGIiMGI:1918019. Nolc1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2992. Eukaryota.
ENOG4111U5P. LUCA.
GeneTreeiENSGT00730000111092.
HOGENOMiHOG000113823.
HOVERGENiHBG075370.
InParanoidiE9Q5C9.
OMAiKKKAGPY.
OrthoDBiEOG091G127U.
TreeFamiTF341730.

Miscellaneous databases

ChiTaRSiNolc1. mouse.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015176.
GenevisibleiE9Q5C9. MM.

Family and domain databases

InterProiView protein in InterPro
IPR006594. LisH.
IPR007718. SRP40_C.
PfamiView protein in Pfam
PF05022. SRP40_C. 1 hit.
PROSITEiView protein in PROSITE
PS50896. LISH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNOLC1_MOUSE
AccessioniPrimary (citable) accession number: E9Q5C9
Secondary accession number(s): Q3TKZ9
, Q3U6W2, Q3UBB6, Q3UI16, Q6ZQK6, Q8CE21
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2017
Last sequence update: April 5, 2011
Last modified: April 12, 2017
This is version 47 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.