E9Q557 (DESP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Desmoplakin
Short name=DP

Gene names

Name:

Dsp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	2883 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.
Subunit structure	Homodimer. Interacts with COL17A1 (via cytoplasmic region). Associates (via C-terminal) with KRT5-KRT14 (via rod region), KRT8-KRT18 and VIM intermediate filaments By similarity.
Subcellular location	Cell junction › desmosome By similarity. Cytoplasm › cytoskeleton By similarity. Note: Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network By similarity.
Domain	Its association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins By similarity. The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1 By similarity. The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments By similarity.
Post-translational modification	Ser-2860 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2860 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments By similarity. Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide By similarity.
Sequence similarities	Belongs to the plakin or cytolinker family. Contains 17 plectin repeats. Contains 1 SH3 domain. Contains 6 spectrin repeats.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasm Cytoskeleton
Disease	Cardiomyopathy
Domain	Coiled coil Repeat
PTM	Lipoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adherens junction organization Inferred from mutant phenotype PubMed 11781569. Source: MGI bundle of His cell to Purkinje myocyte communication Inferred from electronic annotation. Source: Compara cell-cell adhesion Inferred from mutant phenotype PubMed 11781569PubMed 9864371. Source: MGI desmosome organization Inferred from mutant phenotype PubMed 16917092. Source: BHF-UCL intermediate filament organization Inferred from mutant phenotype PubMed 16917092. Source: BHF-UCL keratinocyte differentiation Inferred from electronic annotation. Source: Compara peptide cross-linking Inferred from electronic annotation. Source: Compara protein localization to adherens junction Inferred from mutant phenotype PubMed 16917092. Source: BHF-UCL regulation of heart rate by cardiac conduction Inferred from electronic annotation. Source: Compara regulation of ventricular cardiac muscle cell action potential Inferred from electronic annotation. Source: Compara skin development Inferred from mutant phenotype PubMed 11781569. Source: MGI ventricular compact myocardium morphogenesis Inferred from mutant phenotype PubMed 16917092. Source: BHF-UCL
Cellular_component	basolateral plasma membrane Inferred from direct assay PubMed 12951053. Source: MGI cornified envelope Inferred from electronic annotation. Source: Compara desmosome Inferred from direct assay PubMed 14673151PubMed 16418220PubMed 18496566. Source: MGI intercalated disc Inferred from direct assay PubMed 17535849. Source: MGI mitochondrion Inferred from direct assay PubMed 14651853. Source: MGI
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2883	2883	Desmoplakin		PRO_0000410831
Regions
	Repeat	190 – 283	94	Spectrin 1
	Repeat	284 – 387	104	Spectrin 2
	Repeat	388 – 458	71	Spectrin 3a
	Domain	459 – 527	69	SH3
	Repeat	528 – 557	30	Spectrin 3b
	Repeat	558 – 639	82	Spectrin 4
	Repeat	666 – 781	116	Spectrin 5
	Repeat	782 – 895	114	Spectrin 6
	Repeat	2021 – 2057	37	Plectin 1
	Repeat	2058 – 2095	38	Plectin 2
	Repeat	2096 – 2133	38	Plectin 3
	Repeat	2134 – 2171	38	Plectin 4
	Repeat	2175 – 2209	35	Plectin 5
	Repeat	2210 – 2245	36	Plectin 6
	Repeat	2263 – 2300	38	Plectin 7
	Repeat	2301 – 2338	38	Plectin 8
	Repeat	2339 – 2376	38	Plectin 9
	Repeat	2377 – 2414	38	Plectin 10
	Repeat	2418 – 2452	35	Plectin 11
	Repeat	2468 – 2505	38	Plectin 12
	Repeat	2519 – 2556	38	Plectin 13
	Repeat	2622 – 2659	38	Plectin 14
	Repeat	2660 – 2697	38	Plectin 15
	Repeat	2736 – 2773	38	Plectin 16
	Repeat	2774 – 2811	38	Plectin 17
	Region	1 – 1068	1068	Globular 1
	Region	1 – 596	596	Interacts with plakophilin 1 and junction plakoglobin By similarity
	Region	1069 – 1957	889	Central fibrous rod domain
	Region	1958 – 2882	925	Globular 2
	Region	1972 – 2220	249	4.5 X 38 AA tandem repeats (Domain A)
	Region	2256 – 2458	203	4.5 X 38 AA tandem repeats (Domain B)
	Region	2621 – 2833	213	4.5 X 38 AA tandem repeats (Domain C)
	Region	2835 – 2858	24	6 X 4 AA tandem repeats of G-S-R-[SR]
	Coiled coil	1034 – 1956	923	Potential
Amino acid modifications
	Modified residue	22	1	Phosphoserine By similarity
	Modified residue	177	1	Phosphoserine By similarity
	Modified residue	178	1	Phosphoserine By similarity
	Modified residue	188	1	Phosphoserine By similarity
	Modified residue	2036	1	Phosphoserine By similarity
	Modified residue	2219	1	Phosphoserine By similarity
	Modified residue	2221	1	Phosphoserine By similarity
	Modified residue	2827	1	Phosphoserine By similarity
	Modified residue	2832	1	Phosphoserine By similarity
	Modified residue	2836	1	Phosphoserine By similarity
	Modified residue	2860	1	Phosphoserine By similarity
	Modified residue	2864	1	Phosphothreonine By similarity
	Modified residue	2879	1	Phosphoserine By similarity
	Lipidation	2492	1	Omega-hydroxyceramide glutamate ester By similarity

Sequences

	Sequence		Length	Mass (Da)
	E9Q557 [UniParc]. Last modified April 5, 2011. Version 1. Checksum: 1D8B68788D519940	FASTA	2,883	332,912
	10 20 30 40 50 60 MSCNGGSHPR INTLGRMTRA ESGPDLRYEM TYSGGGGGGG GGGGGGTSRT FYSHSRRCTV 70 80 90 100 110 120 NDQNSDGYCQ TGTMSRHQNQ NTIQEMLQNC SDCLMRAELI AQPELKFGEG MQLAWNRELD 130 140 150 160 170 180 EYFTQANDQM EIIDGLIREM RQMGQPCDAY QKRLLQLQEQ MRALYKAISV PRVRRASSKG 190 200 210 220 230 240 AGGYTCQSGS GWDEFTKRLT GECLGWMRQQ REEMDLMAWG VDAGSVEQHI NSHRSIHNTI 250 260 270 280 290 300 GDYRWQLDKI KADLREKSAI YQLEEEYENL LKASFERMDH LRQLQNIIQA TSREIMWIND 310 320 330 340 350 360 CEEEELLYDW SDKNTNIAQK QEAFSIRMSQ LEVKEKELNK LKQESDQLVL NQHPASDKIE 370 380 390 400 410 420 AYMDTLQTQW SWILQITKCI DVHLKENAAY FQFFEEAQST EAYLKGLQDS IRKKYPCDKN 430 440 450 460 470 480 MPLQHLLEQI KELEKEREKI IEYKRQVQNL VNKSKKIVQL KPRNPDYRSN KPIILRALCD 490 500 510 520 530 540 YKQDQKIVHK GDECILKDNN ERSKWYVTGP GGVDMLVPSV GLIIPPPNPL AVDLSCKIEQ 550 560 570 580 590 600 YYEAILALWN QLYINMKSLV SWHYCMIDIE KIRAMTIAKL KTMRQEDYMK TIEDLELHYQ 610 620 630 640 650 660 DFIKNSQGSE MFGDDDKRRM QSQFTDAQKH YQTLVIQLPG HPQHQTVTKT EITHLGTCQD 670 680 690 700 710 720 VNHNKVIETN RENDKQETWL LMELQKIRRQ MEHCEARMTL KNLLLAEQGS THHITVKINE 730 740 750 760 770 780 LKSVQNDSQA LAEVLNQLKD MLANFRGSEK YCYLQNEIFG LFQKLENING VSDGYLNSLC 790 800 810 820 830 840 SVRALLQAIL QTEDMLKVYE ARLTEEETVC LDLDKVEAYR CGLKKIKNDL NLKKSLLATM 850 860 870 880 890 900 KTELQKAQQI HSQSSQQYPL YDLDLGKFTE KVTQLTDRWQ KIDKQIDFRL WDLEKQIKQL 910 920 930 940 950 960 RNYRDNYQSF CKWLYDAKRR QDSLESMKFG DSNTVMRFLN EQKNLHSEIS GKRDKSEEVH 970 980 990 1000 1010 1020 KIAELCANSI KDYELQLASY TSGLETLLNI PIKRTMVQSP SGVILQEAAD IHARYIELLT 1030 1040 1050 1060 1070 1080 RSGDYYRFLS EMLKSLEDLK LKNTKIEVLE EELRLARDAN SENCNKNKFL DQNLQKYQAE 1090 1100 1110 1120 1130 1140 CSQFKAKLVS LEELKRQAEL DGKSAKQNLD KCYGQIKELN EKITRLTYEI EDEKRRRKTV 1150 1160 1170 1180 1190 1200 EDRFDQQKND YDQLQKARQC EKENLSWQKL ESEKAIKEKE YEIERLRVLL QEEGARKREY 1210 1220 1230 1240 1250 1260 ENELAKVRNH YNEEMSNLRN KYETEINITK TTIKEISMQK EDDSKNLRNQ MDRLSRENRD 1270 1280 1290 1300 1310 1320 LKDEIVRLND SILQATEQRR RAEENALQQK ACGSETMQKK QRLEIELKQV IQQRSEDNAR 1330 1340 1350 1360 1370 1380 HKQSLEEAAK TIQDKNKEIE RLKAEYQEEA KRRWEYENEL SKVRNSYDEE IISLKNQFET 1390 1400 1410 1420 1430 1440 EINITKTTIH QLTMQKEEDT SGYRAQIDNL TRENRSLCEE VKRLKNTLAQ TTENLRRVEE 1450 1460 1470 1480 1490 1500 NAQQQKATGS EMSQRKQQLE IELRQVTQMR TEESMRYKQS LDDAAKTIQD KNKEIERLKQ 1510 1520 1530 1540 1550 1560 LVDKETNERK CLEDENSKLQ RVQYDLQKAN NSATEAMSKL KVQEQELTRL RIDYERVSQE 1570 1580 1590 1600 1610 1620 RTVKDQDITR IQSSLKDLQL QKQKAEEELS RLKRTASDES SKRKMLEEEL EAMRRSLKEQ 1630 1640 1650 1660 1670 1680 AVKITNLTQQ LEQASIVKKR SEDDLRQQRD VLDGHVREKQ RTQEELRRLS LDVEALRRQL 1690 1700 1710 1720 1730 1740 VQEQENVKQA HLRNEHFQKA IEDKSRSLNE SKIEIERLQS LTENLTKEHL MLEEELRNLR 1750 1760 1770 1780 1790 1800 LEYDDLRRGR SEADSDKNST ISELRSQLQI SNNRTLELQG LINDLQRERE NLRQEIEKFQ 1810 1820 1830 1840 1850 1860 KQALEASNRI QESKSQCTQV VQERESLLVK IKVLEQDKAR LQRLEDELNR AKATLEAESR 1870 1880 1890 1900 1910 1920 VKQRLECEKQ QIQNDLNQWK TQYSRKEETI RKIESEREKS EREKNSLRSE IERLQAEIKR 1930 1940 1950 1960 1970 1980 IEERCRRKLE DSSRETQSQL ESERCRLQKE IEKLRQRPYG SHRETQTEYE WTVDSSKLVF 1990 2000 2010 2020 2030 2040 DGLRKKVTAM QLYECQLIDK TTLDKLLKGK KSVEEVASEI QPFLRGAGAI AGASASPKEK 2050 2060 2070 2080 2090 2100 YSLVEAKRKK FITPESTVML LEAQAATGGI IDPHRNEKLT VDNAVARDLI DFDDRQQIYT 2110 2120 2130 2140 2150 2160 AEKAITGFDD PFSGKTVSVS EAIKKNLIDR ETGMRLLEAQ LASGGVVDPV NSVFLPKDVA 2170 2180 2190 2200 2210 2220 LARGLIDRDL YRSLNDPRDS QKNFVDPITK KKVSYMQLRE RCRIEPHTGL LLLSVQKRSM 2230 2240 2250 2260 2270 2280 SFQGIRQPVT VTELVDSGIL RPSTVNELES GQISYDEVGE RIKDFLQGSS CIAGIYNETT 2290 2300 2310 2320 2330 2340 KQKLGIYEAM KIGLVRPGTA LELLEAQAAT GFIVDPVSNL RLPVEEAYKR GLVGIEFKEK 2350 2360 2370 2380 2390 2400 LLSAERAVTG YNDPETGNII SLFQAMNKEL IEKGHGIRLL EAQIATGGII DPKESHRLPV 2410 2420 2430 2440 2450 2460 DMAYKRGYFN EELSEILSDP SDDTKGFFDP NTEENLTYLQ LKERCIKDEE TGLCLLPLKE 2470 2480 2490 2500 2510 2520 KKKQVQTSQK NTLRKRRVVI VDPETNKEMS VQEAYKKGLI DYDTFKELCE QECEWEEITI 2530 2540 2550 2560 2570 2580 TGSDGSTRVV LVDRKTGSQY DIQDAIDKGL VDRKFFDQYR SGSLSLTQFA DMISLKNGVG 2590 2600 2610 2620 2630 2640 NSSGLGGSVN DDVFSSSRHD SVSKISTISS VRNLTIRSSS LSDPLEESSP IAAIFDTENL 2650 2660 2670 2680 2690 2700 EKISIAEGIE RGIVDSITGQ RLLEAQACTG GIIHPTTGQK LSLQDAVNQG LIDQDMATRL 2710 2720 2730 2740 2750 2760 KPAQKAFIGF EGVKGKKKMS AAEAVKEKWL PYEAGQRFLE FQFLTGGLVD PEVHGRISTE 2770 2780 2790 2800 2810 2820 EAIRKGFIDG RAAQRLQDIS SYAKILTCPK TKLKISYKDA MNRSMVEDIT GLRLLEAASV 2830 2840 2850 2860 2870 2880 SSKGLPSPYN MSAPGSRSGS RSGSRSGSRS GSRSGSRRGS FDATGNSSYS YSYSFSSSSI GGY

References

[1]

"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC140331 Genomic DNA. No translation available.
IPI	IPI00553419.
RefSeq	NP_076331.2. NM_023842.2.
UniGene	Mm.355327.
3D structure databases
ProteinModelPortal	E9Q557.
SMR	E9Q557. Positions 190-639, 2221-2460, 2625-2820.
ModBase	Search...
Protein-protein interaction databases
IntAct	E9Q557. 4 interactions.
Proteomic databases
PRIDE	E9Q557.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000124830; ENSMUSP00000115062; ENSMUSG00000054889.
GeneID	109620.
KEGG	mmu:109620.
UCSC	uc007qdk.2. mouse.
Organism-specific databases
CTD	1832.
MGI	MGI:109611. Dsp.
Phylogenomic databases
GeneTree	ENSGT00700000104214.
KO	K10381.
OMA	KRSMSFQ.
Gene expression databases
ArrayExpress	E9Q557.
Bgee	E9Q557.
Family and domain databases
InterPro	IPR001101. Plectin_repeat. IPR018159. Spectrin/alpha-actinin. [Graphical view]
Pfam	PF00681. Plectin. 8 hits. [Graphical view]
SMART	SM00250. PLEC. 18 hits. SM00150. SPEC. 2 hits. [Graphical view]
PROSITE	PS50002. SH3. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DSP. mouse.
NextBio	362451.
SOURCE	Search...

Entry information

Entry name

DESP_MOUSE

Accession

Primary (citable) accession number: E9Q557

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	April 5, 2011
Last modified:	May 29, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program