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Protein

Desmoplakin

Gene

Dsp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.

GO - Molecular functioni

GO - Biological processi

  • adherens junction organization Source: MGI
  • bundle of His cell-Purkinje myocyte adhesion involved in cell communication Source: MGI
  • desmosome organization Source: BHF-UCL
  • intermediate filament cytoskeleton organization Source: MGI
  • intermediate filament organization Source: BHF-UCL
  • keratinocyte differentiation Source: MGI
  • peptide cross-linking Source: MGI
  • protein localization to adherens junction Source: BHF-UCL
  • regulation of heart rate by cardiac conduction Source: MGI
  • regulation of ventricular cardiac muscle cell action potential Source: MGI
  • single organismal cell-cell adhesion Source: MGI
  • skin development Source: MGI
  • ventricular compact myocardium morphogenesis Source: BHF-UCL
  • wound healing Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-6805567. Keratinization.
R-MMU-6809371. Formation of the cornified envelope.

Names & Taxonomyi

Protein namesi
Recommended name:
Desmoplakin
Short name:
DP
Gene namesi
Name:Dsp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:109611. Dsp.

Subcellular locationi

GO - Cellular componenti

  • basolateral plasma membrane Source: MGI
  • cell-cell junction Source: MGI
  • cornified envelope Source: MGI
  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB-SubCell
  • desmosome Source: UniProtKB
  • extracellular exosome Source: MGI
  • extracellular matrix Source: MGI
  • fascia adherens Source: Ensembl
  • intercalated disc Source: UniProtKB
  • membrane Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Keywords - Diseasei

Cardiomyopathy

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004108311 – 2883DesmoplakinAdd BLAST2883

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22PhosphoserineCombined sources1
Modified residuei59PhosphothreonineCombined sources1
Modified residuei65PhosphoserineCombined sources1
Modified residuei68PhosphotyrosineCombined sources1
Modified residuei73PhosphothreonineCombined sources1
Modified residuei177PhosphoserineBy similarity1
Modified residuei178PhosphoserineBy similarity1
Modified residuei188PhosphoserineBy similarity1
Modified residuei1670PhosphoserineBy similarity1
Modified residuei1720PhosphoserineBy similarity1
Modified residuei2036PhosphoserineBy similarity1
Modified residuei2219PhosphoserineBy similarity1
Modified residuei2221PhosphoserineCombined sources1
Modified residuei2237PhosphoserineBy similarity1
Lipidationi2492Omega-hydroxyceramide glutamate esterBy similarity1
Modified residuei2822PhosphoserineBy similarity1
Modified residuei2827PhosphoserineBy similarity1
Modified residuei2829PhosphotyrosineBy similarity1
Modified residuei2832PhosphoserineCombined sources1
Modified residuei2836PhosphoserineCombined sources1
Modified residuei2837Omega-N-methylarginineCombined sources1
Modified residuei2858Omega-N-methylarginineCombined sources1
Modified residuei2860PhosphoserineBy similarity1
Modified residuei2864PhosphothreonineBy similarity1
Modified residuei2879PhosphoserineBy similarity1

Post-translational modificationi

Ser-2860 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2860 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments (By similarity).By similarity
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein

Proteomic databases

MaxQBiE9Q557.
PaxDbiE9Q557.
PeptideAtlasiE9Q557.
PRIDEiE9Q557.

PTM databases

iPTMnetiE9Q557.
PhosphoSitePlusiE9Q557.
SwissPalmiE9Q557.

Expressioni

Gene expression databases

BgeeiENSMUSG00000054889.
ExpressionAtlasiE9Q557. baseline and differential.
GenevisibleiE9Q557. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with COL17A1 (via cytoplasmic region). Associates (via C-terminal) with KRT5-KRT14 (via rod region), KRT8-KRT18 and VIM intermediate filaments. Interacts with PKP2 (By similarity). Interacts weakly with TMEM65 (PubMed:26403541).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi224908. 3 interactors.
IntActiE9Q557. 6 interactors.
STRINGi10090.ENSMUSP00000115062.

Structurei

3D structure databases

ProteinModelPortaliE9Q557.
SMRiE9Q557.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati190 – 283Spectrin 1Add BLAST94
Repeati284 – 387Spectrin 2Add BLAST104
Repeati388 – 458Spectrin 3aAdd BLAST71
Domaini459 – 527SH3Add BLAST69
Repeati528 – 557Spectrin 3bAdd BLAST30
Repeati558 – 639Spectrin 4Add BLAST82
Repeati666 – 781Spectrin 5Add BLAST116
Repeati782 – 895Spectrin 6Add BLAST114
Repeati2021 – 2057Plectin 1Add BLAST37
Repeati2058 – 2095Plectin 2Add BLAST38
Repeati2096 – 2133Plectin 3Add BLAST38
Repeati2134 – 2171Plectin 4Add BLAST38
Repeati2175 – 2209Plectin 5Add BLAST35
Repeati2210 – 2245Plectin 6Add BLAST36
Repeati2263 – 2300Plectin 7Add BLAST38
Repeati2301 – 2338Plectin 8Add BLAST38
Repeati2339 – 2376Plectin 9Add BLAST38
Repeati2377 – 2414Plectin 10Add BLAST38
Repeati2418 – 2452Plectin 11Add BLAST35
Repeati2468 – 2505Plectin 12Add BLAST38
Repeati2519 – 2556Plectin 13Add BLAST38
Repeati2622 – 2659Plectin 14Add BLAST38
Repeati2660 – 2697Plectin 15Add BLAST38
Repeati2736 – 2773Plectin 16Add BLAST38
Repeati2774 – 2811Plectin 17Add BLAST38

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 1068Globular 1Add BLAST1068
Regioni1 – 596Interaction with plakophilin 1 and junction plakoglobinBy similarityAdd BLAST596
Regioni1069 – 1957Central fibrous rod domainAdd BLAST889
Regioni1958 – 2882Globular 2Add BLAST925
Regioni1972 – 22204.5 X 38 AA tandem repeats (Domain A)Add BLAST249
Regioni2256 – 24584.5 X 38 AA tandem repeats (Domain B)Add BLAST203
Regioni2621 – 28334.5 X 38 AA tandem repeats (Domain C)Add BLAST213
Regioni2835 – 28586 X 4 AA tandem repeats of G-S-R-[SR]Add BLAST24

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1034 – 1956Sequence analysisAdd BLAST923

Domaini

Its association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.By similarity
The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.By similarity
The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments.By similarity

Sequence similaritiesi

Belongs to the plakin or cytolinker family.Curated
Contains 17 plectin repeats.Curated
Contains 1 SH3 domain.Curated
Contains 6 spectrin repeats.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410KD4I. Eukaryota.
ENOG4111ACS. LUCA.
GeneTreeiENSGT00760000119163.
InParanoidiE9Q557.
KOiK10381.
OMAiKRSMSFQ.
OrthoDBiEOG091G003R.
TreeFamiTF106435.

Family and domain databases

Gene3Di3.90.1290.10. 4 hits.
InterProiIPR028462. Desmoplakin.
IPR001101. Plectin_repeat.
IPR018159. Spectrin/alpha-actinin.
[Graphical view]
PANTHERiPTHR11915:SF234. PTHR11915:SF234. 1 hit.
PfamiPF00681. Plectin. 8 hits.
[Graphical view]
SMARTiSM00250. PLEC. 18 hits.
SM00150. SPEC. 2 hits.
[Graphical view]
SUPFAMiSSF75399. SSF75399. 5 hits.

Sequencei

Sequence statusi: Complete.

E9Q557-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCNGGSHPR INTLGRMTRA ESGPDLRYEM TYSGGGGGGG GGGGGGTSRT
60 70 80 90 100
FYSHSRRCTV NDQNSDGYCQ TGTMSRHQNQ NTIQEMLQNC SDCLMRAELI
110 120 130 140 150
AQPELKFGEG MQLAWNRELD EYFTQANDQM EIIDGLIREM RQMGQPCDAY
160 170 180 190 200
QKRLLQLQEQ MRALYKAISV PRVRRASSKG AGGYTCQSGS GWDEFTKRLT
210 220 230 240 250
GECLGWMRQQ REEMDLMAWG VDAGSVEQHI NSHRSIHNTI GDYRWQLDKI
260 270 280 290 300
KADLREKSAI YQLEEEYENL LKASFERMDH LRQLQNIIQA TSREIMWIND
310 320 330 340 350
CEEEELLYDW SDKNTNIAQK QEAFSIRMSQ LEVKEKELNK LKQESDQLVL
360 370 380 390 400
NQHPASDKIE AYMDTLQTQW SWILQITKCI DVHLKENAAY FQFFEEAQST
410 420 430 440 450
EAYLKGLQDS IRKKYPCDKN MPLQHLLEQI KELEKEREKI IEYKRQVQNL
460 470 480 490 500
VNKSKKIVQL KPRNPDYRSN KPIILRALCD YKQDQKIVHK GDECILKDNN
510 520 530 540 550
ERSKWYVTGP GGVDMLVPSV GLIIPPPNPL AVDLSCKIEQ YYEAILALWN
560 570 580 590 600
QLYINMKSLV SWHYCMIDIE KIRAMTIAKL KTMRQEDYMK TIEDLELHYQ
610 620 630 640 650
DFIKNSQGSE MFGDDDKRRM QSQFTDAQKH YQTLVIQLPG HPQHQTVTKT
660 670 680 690 700
EITHLGTCQD VNHNKVIETN RENDKQETWL LMELQKIRRQ MEHCEARMTL
710 720 730 740 750
KNLLLAEQGS THHITVKINE LKSVQNDSQA LAEVLNQLKD MLANFRGSEK
760 770 780 790 800
YCYLQNEIFG LFQKLENING VSDGYLNSLC SVRALLQAIL QTEDMLKVYE
810 820 830 840 850
ARLTEEETVC LDLDKVEAYR CGLKKIKNDL NLKKSLLATM KTELQKAQQI
860 870 880 890 900
HSQSSQQYPL YDLDLGKFTE KVTQLTDRWQ KIDKQIDFRL WDLEKQIKQL
910 920 930 940 950
RNYRDNYQSF CKWLYDAKRR QDSLESMKFG DSNTVMRFLN EQKNLHSEIS
960 970 980 990 1000
GKRDKSEEVH KIAELCANSI KDYELQLASY TSGLETLLNI PIKRTMVQSP
1010 1020 1030 1040 1050
SGVILQEAAD IHARYIELLT RSGDYYRFLS EMLKSLEDLK LKNTKIEVLE
1060 1070 1080 1090 1100
EELRLARDAN SENCNKNKFL DQNLQKYQAE CSQFKAKLVS LEELKRQAEL
1110 1120 1130 1140 1150
DGKSAKQNLD KCYGQIKELN EKITRLTYEI EDEKRRRKTV EDRFDQQKND
1160 1170 1180 1190 1200
YDQLQKARQC EKENLSWQKL ESEKAIKEKE YEIERLRVLL QEEGARKREY
1210 1220 1230 1240 1250
ENELAKVRNH YNEEMSNLRN KYETEINITK TTIKEISMQK EDDSKNLRNQ
1260 1270 1280 1290 1300
MDRLSRENRD LKDEIVRLND SILQATEQRR RAEENALQQK ACGSETMQKK
1310 1320 1330 1340 1350
QRLEIELKQV IQQRSEDNAR HKQSLEEAAK TIQDKNKEIE RLKAEYQEEA
1360 1370 1380 1390 1400
KRRWEYENEL SKVRNSYDEE IISLKNQFET EINITKTTIH QLTMQKEEDT
1410 1420 1430 1440 1450
SGYRAQIDNL TRENRSLCEE VKRLKNTLAQ TTENLRRVEE NAQQQKATGS
1460 1470 1480 1490 1500
EMSQRKQQLE IELRQVTQMR TEESMRYKQS LDDAAKTIQD KNKEIERLKQ
1510 1520 1530 1540 1550
LVDKETNERK CLEDENSKLQ RVQYDLQKAN NSATEAMSKL KVQEQELTRL
1560 1570 1580 1590 1600
RIDYERVSQE RTVKDQDITR IQSSLKDLQL QKQKAEEELS RLKRTASDES
1610 1620 1630 1640 1650
SKRKMLEEEL EAMRRSLKEQ AVKITNLTQQ LEQASIVKKR SEDDLRQQRD
1660 1670 1680 1690 1700
VLDGHVREKQ RTQEELRRLS LDVEALRRQL VQEQENVKQA HLRNEHFQKA
1710 1720 1730 1740 1750
IEDKSRSLNE SKIEIERLQS LTENLTKEHL MLEEELRNLR LEYDDLRRGR
1760 1770 1780 1790 1800
SEADSDKNST ISELRSQLQI SNNRTLELQG LINDLQRERE NLRQEIEKFQ
1810 1820 1830 1840 1850
KQALEASNRI QESKSQCTQV VQERESLLVK IKVLEQDKAR LQRLEDELNR
1860 1870 1880 1890 1900
AKATLEAESR VKQRLECEKQ QIQNDLNQWK TQYSRKEETI RKIESEREKS
1910 1920 1930 1940 1950
EREKNSLRSE IERLQAEIKR IEERCRRKLE DSSRETQSQL ESERCRLQKE
1960 1970 1980 1990 2000
IEKLRQRPYG SHRETQTEYE WTVDSSKLVF DGLRKKVTAM QLYECQLIDK
2010 2020 2030 2040 2050
TTLDKLLKGK KSVEEVASEI QPFLRGAGAI AGASASPKEK YSLVEAKRKK
2060 2070 2080 2090 2100
FITPESTVML LEAQAATGGI IDPHRNEKLT VDNAVARDLI DFDDRQQIYT
2110 2120 2130 2140 2150
AEKAITGFDD PFSGKTVSVS EAIKKNLIDR ETGMRLLEAQ LASGGVVDPV
2160 2170 2180 2190 2200
NSVFLPKDVA LARGLIDRDL YRSLNDPRDS QKNFVDPITK KKVSYMQLRE
2210 2220 2230 2240 2250
RCRIEPHTGL LLLSVQKRSM SFQGIRQPVT VTELVDSGIL RPSTVNELES
2260 2270 2280 2290 2300
GQISYDEVGE RIKDFLQGSS CIAGIYNETT KQKLGIYEAM KIGLVRPGTA
2310 2320 2330 2340 2350
LELLEAQAAT GFIVDPVSNL RLPVEEAYKR GLVGIEFKEK LLSAERAVTG
2360 2370 2380 2390 2400
YNDPETGNII SLFQAMNKEL IEKGHGIRLL EAQIATGGII DPKESHRLPV
2410 2420 2430 2440 2450
DMAYKRGYFN EELSEILSDP SDDTKGFFDP NTEENLTYLQ LKERCIKDEE
2460 2470 2480 2490 2500
TGLCLLPLKE KKKQVQTSQK NTLRKRRVVI VDPETNKEMS VQEAYKKGLI
2510 2520 2530 2540 2550
DYDTFKELCE QECEWEEITI TGSDGSTRVV LVDRKTGSQY DIQDAIDKGL
2560 2570 2580 2590 2600
VDRKFFDQYR SGSLSLTQFA DMISLKNGVG NSSGLGGSVN DDVFSSSRHD
2610 2620 2630 2640 2650
SVSKISTISS VRNLTIRSSS LSDPLEESSP IAAIFDTENL EKISIAEGIE
2660 2670 2680 2690 2700
RGIVDSITGQ RLLEAQACTG GIIHPTTGQK LSLQDAVNQG LIDQDMATRL
2710 2720 2730 2740 2750
KPAQKAFIGF EGVKGKKKMS AAEAVKEKWL PYEAGQRFLE FQFLTGGLVD
2760 2770 2780 2790 2800
PEVHGRISTE EAIRKGFIDG RAAQRLQDIS SYAKILTCPK TKLKISYKDA
2810 2820 2830 2840 2850
MNRSMVEDIT GLRLLEAASV SSKGLPSPYN MSAPGSRSGS RSGSRSGSRS
2860 2870 2880
GSRSGSRRGS FDATGNSSYS YSYSFSSSSI GGY
Length:2,883
Mass (Da):332,912
Last modified:April 5, 2011 - v1
Checksum:i1D8B68788D519940
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC140331 Genomic DNA. No translation available.
CCDSiCCDS49239.1.
RefSeqiNP_076331.2. NM_023842.2.
UniGeneiMm.355327.

Genome annotation databases

EnsembliENSMUST00000124830; ENSMUSP00000115062; ENSMUSG00000054889.
GeneIDi109620.
KEGGimmu:109620.
UCSCiuc007qdk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC140331 Genomic DNA. No translation available.
CCDSiCCDS49239.1.
RefSeqiNP_076331.2. NM_023842.2.
UniGeneiMm.355327.

3D structure databases

ProteinModelPortaliE9Q557.
SMRiE9Q557.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi224908. 3 interactors.
IntActiE9Q557. 6 interactors.
STRINGi10090.ENSMUSP00000115062.

PTM databases

iPTMnetiE9Q557.
PhosphoSitePlusiE9Q557.
SwissPalmiE9Q557.

Proteomic databases

MaxQBiE9Q557.
PaxDbiE9Q557.
PeptideAtlasiE9Q557.
PRIDEiE9Q557.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000124830; ENSMUSP00000115062; ENSMUSG00000054889.
GeneIDi109620.
KEGGimmu:109620.
UCSCiuc007qdk.2. mouse.

Organism-specific databases

CTDi1832.
MGIiMGI:109611. Dsp.

Phylogenomic databases

eggNOGiENOG410KD4I. Eukaryota.
ENOG4111ACS. LUCA.
GeneTreeiENSGT00760000119163.
InParanoidiE9Q557.
KOiK10381.
OMAiKRSMSFQ.
OrthoDBiEOG091G003R.
TreeFamiTF106435.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-6805567. Keratinization.
R-MMU-6809371. Formation of the cornified envelope.

Miscellaneous databases

ChiTaRSiDsp. mouse.
PROiE9Q557.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054889.
ExpressionAtlasiE9Q557. baseline and differential.
GenevisibleiE9Q557. MM.

Family and domain databases

Gene3Di3.90.1290.10. 4 hits.
InterProiIPR028462. Desmoplakin.
IPR001101. Plectin_repeat.
IPR018159. Spectrin/alpha-actinin.
[Graphical view]
PANTHERiPTHR11915:SF234. PTHR11915:SF234. 1 hit.
PfamiPF00681. Plectin. 8 hits.
[Graphical view]
SMARTiSM00250. PLEC. 18 hits.
SM00150. SPEC. 2 hits.
[Graphical view]
SUPFAMiSSF75399. SSF75399. 5 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiDESP_MOUSE
AccessioniPrimary (citable) accession number: E9Q557
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: April 5, 2011
Last modified: November 30, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.