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E9Q557 (DESP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Desmoplakin

Short name=DP
Gene names
Name:Dsp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2883 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.

Subunit structure

Homodimer. Interacts with COL17A1 (via cytoplasmic region). Associates (via C-terminal) with KRT5-KRT14 (via rod region), KRT8-KRT18 and VIM intermediate filaments. Interacts with PKP2 By similarity.

Subcellular location

Cell junctiondesmosome By similarity. Cytoplasmcytoskeleton By similarity. Note: Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network By similarity.

Domain

Its association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins By similarity.

The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1 By similarity.

The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments By similarity.

Post-translational modification

Ser-2860 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2860 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments By similarity.

Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide By similarity.

Sequence similarities

Belongs to the plakin or cytolinker family.

Contains 17 plectin repeats.

Contains 1 SH3 domain.

Contains 6 spectrin repeats.

Ontologies

Keywords
   Cellular componentCell junction
Cytoplasm
Cytoskeleton
   DiseaseCardiomyopathy
   DomainCoiled coil
Repeat
   PTMLipoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadherens junction organization

Inferred from mutant phenotype PubMed 11781569. Source: MGI

bundle of His cell to Purkinje myocyte communication

Inferred from electronic annotation. Source: Ensembl

cell-cell adhesion

Inferred from mutant phenotype PubMed 11781569PubMed 9864371. Source: MGI

desmosome organization

Inferred from mutant phenotype PubMed 16917092. Source: BHF-UCL

intermediate filament cytoskeleton organization

Inferred from mutant phenotype PubMed 9864371. Source: MGI

intermediate filament organization

Inferred from mutant phenotype PubMed 16917092. Source: BHF-UCL

keratinocyte differentiation

Inferred from electronic annotation. Source: Ensembl

peptide cross-linking

Inferred from electronic annotation. Source: Ensembl

protein localization to adherens junction

Inferred from mutant phenotype PubMed 16917092. Source: BHF-UCL

regulation of heart rate by cardiac conduction

Inferred from electronic annotation. Source: Ensembl

skin development

Inferred from mutant phenotype PubMed 11781569. Source: MGI

ventricular cardiac muscle cell action potential

Inferred from electronic annotation. Source: Ensembl

ventricular compact myocardium morphogenesis

Inferred from mutant phenotype PubMed 16917092. Source: BHF-UCL

wound healing

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentbasolateral plasma membrane

Inferred from direct assay PubMed 12951053. Source: MGI

cell-cell junction

Inferred from direct assay PubMed 9864371. Source: MGI

cornified envelope

Inferred from electronic annotation. Source: Ensembl

desmosome

Inferred from direct assay PubMed 14673151PubMed 16418220PubMed 18496566. Source: MGI

fascia adherens

Inferred from electronic annotation. Source: Ensembl

intercalated disc

Inferred from direct assay PubMed 17535849. Source: MGI

membrane

Inferred from sequence orthology PubMed 19723622. Source: MGI

mitochondrion

Inferred from direct assay PubMed 14651853. Source: MGI

   Molecular_functionstructural molecule activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 28832883Desmoplakin
PRO_0000410831

Regions

Repeat190 – 28394Spectrin 1
Repeat284 – 387104Spectrin 2
Repeat388 – 45871Spectrin 3a
Domain459 – 52769SH3
Repeat528 – 55730Spectrin 3b
Repeat558 – 63982Spectrin 4
Repeat666 – 781116Spectrin 5
Repeat782 – 895114Spectrin 6
Repeat2021 – 205737Plectin 1
Repeat2058 – 209538Plectin 2
Repeat2096 – 213338Plectin 3
Repeat2134 – 217138Plectin 4
Repeat2175 – 220935Plectin 5
Repeat2210 – 224536Plectin 6
Repeat2263 – 230038Plectin 7
Repeat2301 – 233838Plectin 8
Repeat2339 – 237638Plectin 9
Repeat2377 – 241438Plectin 10
Repeat2418 – 245235Plectin 11
Repeat2468 – 250538Plectin 12
Repeat2519 – 255638Plectin 13
Repeat2622 – 265938Plectin 14
Repeat2660 – 269738Plectin 15
Repeat2736 – 277338Plectin 16
Repeat2774 – 281138Plectin 17
Region1 – 10681068Globular 1
Region1 – 596596Interacts with plakophilin 1 and junction plakoglobin By similarity
Region1069 – 1957889Central fibrous rod domain
Region1958 – 2882925Globular 2
Region1972 – 22202494.5 X 38 AA tandem repeats (Domain A)
Region2256 – 24582034.5 X 38 AA tandem repeats (Domain B)
Region2621 – 28332134.5 X 38 AA tandem repeats (Domain C)
Region2835 – 2858246 X 4 AA tandem repeats of G-S-R-[SR]
Coiled coil1034 – 1956923 Potential

Amino acid modifications

Modified residue221Phosphoserine By similarity
Modified residue1771Phosphoserine By similarity
Modified residue1781Phosphoserine By similarity
Modified residue1881Phosphoserine By similarity
Modified residue20361Phosphoserine By similarity
Modified residue22191Phosphoserine By similarity
Modified residue22211Phosphoserine By similarity
Modified residue28271Phosphoserine By similarity
Modified residue28321Phosphoserine By similarity
Modified residue28361Phosphoserine Ref.2
Modified residue28601Phosphoserine By similarity
Modified residue28641Phosphothreonine By similarity
Modified residue28791Phosphoserine By similarity
Lipidation24921Omega-hydroxyceramide glutamate ester By similarity

Sequences

Sequence LengthMass (Da)Tools
E9Q557 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: 1D8B68788D519940

FASTA2,883332,912
        10         20         30         40         50         60 
MSCNGGSHPR INTLGRMTRA ESGPDLRYEM TYSGGGGGGG GGGGGGTSRT FYSHSRRCTV 

        70         80         90        100        110        120 
NDQNSDGYCQ TGTMSRHQNQ NTIQEMLQNC SDCLMRAELI AQPELKFGEG MQLAWNRELD 

       130        140        150        160        170        180 
EYFTQANDQM EIIDGLIREM RQMGQPCDAY QKRLLQLQEQ MRALYKAISV PRVRRASSKG 

       190        200        210        220        230        240 
AGGYTCQSGS GWDEFTKRLT GECLGWMRQQ REEMDLMAWG VDAGSVEQHI NSHRSIHNTI 

       250        260        270        280        290        300 
GDYRWQLDKI KADLREKSAI YQLEEEYENL LKASFERMDH LRQLQNIIQA TSREIMWIND 

       310        320        330        340        350        360 
CEEEELLYDW SDKNTNIAQK QEAFSIRMSQ LEVKEKELNK LKQESDQLVL NQHPASDKIE 

       370        380        390        400        410        420 
AYMDTLQTQW SWILQITKCI DVHLKENAAY FQFFEEAQST EAYLKGLQDS IRKKYPCDKN 

       430        440        450        460        470        480 
MPLQHLLEQI KELEKEREKI IEYKRQVQNL VNKSKKIVQL KPRNPDYRSN KPIILRALCD 

       490        500        510        520        530        540 
YKQDQKIVHK GDECILKDNN ERSKWYVTGP GGVDMLVPSV GLIIPPPNPL AVDLSCKIEQ 

       550        560        570        580        590        600 
YYEAILALWN QLYINMKSLV SWHYCMIDIE KIRAMTIAKL KTMRQEDYMK TIEDLELHYQ 

       610        620        630        640        650        660 
DFIKNSQGSE MFGDDDKRRM QSQFTDAQKH YQTLVIQLPG HPQHQTVTKT EITHLGTCQD 

       670        680        690        700        710        720 
VNHNKVIETN RENDKQETWL LMELQKIRRQ MEHCEARMTL KNLLLAEQGS THHITVKINE 

       730        740        750        760        770        780 
LKSVQNDSQA LAEVLNQLKD MLANFRGSEK YCYLQNEIFG LFQKLENING VSDGYLNSLC 

       790        800        810        820        830        840 
SVRALLQAIL QTEDMLKVYE ARLTEEETVC LDLDKVEAYR CGLKKIKNDL NLKKSLLATM 

       850        860        870        880        890        900 
KTELQKAQQI HSQSSQQYPL YDLDLGKFTE KVTQLTDRWQ KIDKQIDFRL WDLEKQIKQL 

       910        920        930        940        950        960 
RNYRDNYQSF CKWLYDAKRR QDSLESMKFG DSNTVMRFLN EQKNLHSEIS GKRDKSEEVH 

       970        980        990       1000       1010       1020 
KIAELCANSI KDYELQLASY TSGLETLLNI PIKRTMVQSP SGVILQEAAD IHARYIELLT 

      1030       1040       1050       1060       1070       1080 
RSGDYYRFLS EMLKSLEDLK LKNTKIEVLE EELRLARDAN SENCNKNKFL DQNLQKYQAE 

      1090       1100       1110       1120       1130       1140 
CSQFKAKLVS LEELKRQAEL DGKSAKQNLD KCYGQIKELN EKITRLTYEI EDEKRRRKTV 

      1150       1160       1170       1180       1190       1200 
EDRFDQQKND YDQLQKARQC EKENLSWQKL ESEKAIKEKE YEIERLRVLL QEEGARKREY 

      1210       1220       1230       1240       1250       1260 
ENELAKVRNH YNEEMSNLRN KYETEINITK TTIKEISMQK EDDSKNLRNQ MDRLSRENRD 

      1270       1280       1290       1300       1310       1320 
LKDEIVRLND SILQATEQRR RAEENALQQK ACGSETMQKK QRLEIELKQV IQQRSEDNAR 

      1330       1340       1350       1360       1370       1380 
HKQSLEEAAK TIQDKNKEIE RLKAEYQEEA KRRWEYENEL SKVRNSYDEE IISLKNQFET 

      1390       1400       1410       1420       1430       1440 
EINITKTTIH QLTMQKEEDT SGYRAQIDNL TRENRSLCEE VKRLKNTLAQ TTENLRRVEE 

      1450       1460       1470       1480       1490       1500 
NAQQQKATGS EMSQRKQQLE IELRQVTQMR TEESMRYKQS LDDAAKTIQD KNKEIERLKQ 

      1510       1520       1530       1540       1550       1560 
LVDKETNERK CLEDENSKLQ RVQYDLQKAN NSATEAMSKL KVQEQELTRL RIDYERVSQE 

      1570       1580       1590       1600       1610       1620 
RTVKDQDITR IQSSLKDLQL QKQKAEEELS RLKRTASDES SKRKMLEEEL EAMRRSLKEQ 

      1630       1640       1650       1660       1670       1680 
AVKITNLTQQ LEQASIVKKR SEDDLRQQRD VLDGHVREKQ RTQEELRRLS LDVEALRRQL 

      1690       1700       1710       1720       1730       1740 
VQEQENVKQA HLRNEHFQKA IEDKSRSLNE SKIEIERLQS LTENLTKEHL MLEEELRNLR 

      1750       1760       1770       1780       1790       1800 
LEYDDLRRGR SEADSDKNST ISELRSQLQI SNNRTLELQG LINDLQRERE NLRQEIEKFQ 

      1810       1820       1830       1840       1850       1860 
KQALEASNRI QESKSQCTQV VQERESLLVK IKVLEQDKAR LQRLEDELNR AKATLEAESR 

      1870       1880       1890       1900       1910       1920 
VKQRLECEKQ QIQNDLNQWK TQYSRKEETI RKIESEREKS EREKNSLRSE IERLQAEIKR 

      1930       1940       1950       1960       1970       1980 
IEERCRRKLE DSSRETQSQL ESERCRLQKE IEKLRQRPYG SHRETQTEYE WTVDSSKLVF 

      1990       2000       2010       2020       2030       2040 
DGLRKKVTAM QLYECQLIDK TTLDKLLKGK KSVEEVASEI QPFLRGAGAI AGASASPKEK 

      2050       2060       2070       2080       2090       2100 
YSLVEAKRKK FITPESTVML LEAQAATGGI IDPHRNEKLT VDNAVARDLI DFDDRQQIYT 

      2110       2120       2130       2140       2150       2160 
AEKAITGFDD PFSGKTVSVS EAIKKNLIDR ETGMRLLEAQ LASGGVVDPV NSVFLPKDVA 

      2170       2180       2190       2200       2210       2220 
LARGLIDRDL YRSLNDPRDS QKNFVDPITK KKVSYMQLRE RCRIEPHTGL LLLSVQKRSM 

      2230       2240       2250       2260       2270       2280 
SFQGIRQPVT VTELVDSGIL RPSTVNELES GQISYDEVGE RIKDFLQGSS CIAGIYNETT 

      2290       2300       2310       2320       2330       2340 
KQKLGIYEAM KIGLVRPGTA LELLEAQAAT GFIVDPVSNL RLPVEEAYKR GLVGIEFKEK 

      2350       2360       2370       2380       2390       2400 
LLSAERAVTG YNDPETGNII SLFQAMNKEL IEKGHGIRLL EAQIATGGII DPKESHRLPV 

      2410       2420       2430       2440       2450       2460 
DMAYKRGYFN EELSEILSDP SDDTKGFFDP NTEENLTYLQ LKERCIKDEE TGLCLLPLKE 

      2470       2480       2490       2500       2510       2520 
KKKQVQTSQK NTLRKRRVVI VDPETNKEMS VQEAYKKGLI DYDTFKELCE QECEWEEITI 

      2530       2540       2550       2560       2570       2580 
TGSDGSTRVV LVDRKTGSQY DIQDAIDKGL VDRKFFDQYR SGSLSLTQFA DMISLKNGVG 

      2590       2600       2610       2620       2630       2640 
NSSGLGGSVN DDVFSSSRHD SVSKISTISS VRNLTIRSSS LSDPLEESSP IAAIFDTENL 

      2650       2660       2670       2680       2690       2700 
EKISIAEGIE RGIVDSITGQ RLLEAQACTG GIIHPTTGQK LSLQDAVNQG LIDQDMATRL 

      2710       2720       2730       2740       2750       2760 
KPAQKAFIGF EGVKGKKKMS AAEAVKEKWL PYEAGQRFLE FQFLTGGLVD PEVHGRISTE 

      2770       2780       2790       2800       2810       2820 
EAIRKGFIDG RAAQRLQDIS SYAKILTCPK TKLKISYKDA MNRSMVEDIT GLRLLEAASV 

      2830       2840       2850       2860       2870       2880 
SSKGLPSPYN MSAPGSRSGS RSGSRSGSRS GSRSGSRRGS FDATGNSSYS YSYSFSSSSI 


GGY 

« Hide

References

[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2836, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC140331 Genomic DNA. No translation available.
RefSeqNP_076331.2. NM_023842.2.
UniGeneMm.355327.

3D structure databases

ProteinModelPortalE9Q557.
SMRE9Q557. Positions 190-639, 2221-2460, 2625-2820.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid224908. 2 interactions.
IntActE9Q557. 4 interactions.

Proteomic databases

PRIDEE9Q557.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000124830; ENSMUSP00000115062; ENSMUSG00000054889.
GeneID109620.
KEGGmmu:109620.
UCSCuc007qdk.2. mouse.

Organism-specific databases

CTD1832.
MGIMGI:109611. Dsp.

Phylogenomic databases

GeneTreeENSGT00720000108730.
KOK10381.
OMAKRSMSFQ.
TreeFamTF106435.

Gene expression databases

ArrayExpressE9Q557.
BgeeE9Q557.

Family and domain databases

Gene3D3.90.1290.10. 4 hits.
InterProIPR028462. Desmoplakin.
IPR001101. Plectin_repeat.
IPR018159. Spectrin/alpha-actinin.
[Graphical view]
PANTHERPTHR11915:SF22. PTHR11915:SF22. 1 hit.
PfamPF00681. Plectin. 8 hits.
[Graphical view]
SMARTSM00250. PLEC. 18 hits.
SM00150. SPEC. 2 hits.
[Graphical view]
SUPFAMSSF75399. SSF75399. 5 hits.
ProtoNetSearch...

Other

ChiTaRSDSP. mouse.
NextBio362451.
PROE9Q557.
SOURCESearch...

Entry information

Entry nameDESP_MOUSE
AccessionPrimary (citable) accession number: E9Q557
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: April 5, 2011
Last modified: April 16, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot