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Protein

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B

Gene

Pde8b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland (By similarity).By similarity

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Pathwayi: 3',5'-cyclic AMP degradation

This protein is involved in step 1 of the subpathway that synthesizes AMP from 3',5'-cyclic AMP.
Proteins known to be involved in this subpathway in this organism are:
  1. High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B (Pde8b), cAMP-specific 3',5'-cyclic phosphodiesterase 4A (Pde4a), cAMP-specific 3',5'-cyclic phosphodiesterase 4C (Pde4c), High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A (Pde8a), High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A (Pde7a), cAMP-specific 3',5'-cyclic phosphodiesterase 4D (Pde4d), cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A (Pde10a), cAMP-specific 3',5'-cyclic phosphodiesterase 7B (Pde7b)
This subpathway is part of the pathway 3',5'-cyclic AMP degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from 3',5'-cyclic AMP, the pathway 3',5'-cyclic AMP degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei595Proton donorBy similarity1
Metal bindingi599Divalent metal cation 1; via tele nitrogenBy similarity1
Metal bindingi635Divalent metal cation 1; via tele nitrogenBy similarity1
Metal bindingi636Divalent metal cation 1By similarity1
Metal bindingi636Divalent metal cation 2By similarity1
Metal bindingi761Divalent metal cation 1By similarity1

GO - Molecular functioni

  • 3',5'-cyclic-AMP phosphodiesterase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • behavioral fear response Source: MGI
  • cAMP catabolic process Source: UniProtKB-UniPathway
  • negative regulation of steroid hormone biosynthetic process Source: MGI
  • neuromuscular process controlling balance Source: MGI
  • operant conditioning Source: MGI
  • signal transduction Source: InterPro
  • visual learning Source: MGI

Keywordsi

Molecular functionHydrolase
LigandcAMP, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-418555 G alpha (s) signalling events
UniPathwayiUPA00762; UER00747

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B (EC:3.1.4.53)
Short name:
PDE8B
Alternative name(s):
Cell proliferation-inducing gene 22 protein
Gene namesi
Name:Pde8b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2443999 Pde8b

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004142251 – 865High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8BAdd BLAST865

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei497PhosphoserineCombined sources1
Modified residuei731PhosphoserineCombined sources1
Modified residuei734PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiE9Q4S1
PaxDbiE9Q4S1
PRIDEiE9Q4S1

PTM databases

iPTMnetiE9Q4S1
PhosphoSitePlusiE9Q4S1
SwissPalmiE9Q4S1

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Detectable in the adrenal gland of newborn animals.1 Publication

Gene expression databases

BgeeiENSMUSG00000021684
ExpressionAtlasiE9Q4S1 baseline and differential
GenevisibleiE9Q4S1 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000124068

Structurei

3D structure databases

ProteinModelPortaliE9Q4S1
SMRiE9Q4S1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini247 – 318PASPROSITE-ProRule annotationAdd BLAST72
Domaini519 – 855PDEasePROSITE-ProRule annotationAdd BLAST337

Domaini

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1229 Eukaryota
ENOG410XP9B LUCA
GeneTreeiENSGT00760000118889
InParanoidiE9Q4S1
OMAiPHSFRYK
OrthoDBiEOG091G0337

Family and domain databases

CDDicd00077 HDc, 1 hit
cd00130 PAS, 1 hit
Gene3Di1.10.1300.10, 1 hit
InterProiView protein in InterPro
IPR003607 HD/PDEase_dom
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR023088 PDEase
IPR002073 PDEase_catalytic_dom
IPR036971 PDEase_catalytic_dom_sf
IPR023174 PDEase_CS
IPR013938 PDEase_PDE8
PfamiView protein in Pfam
PF13426 PAS_9, 1 hit
PF08629 PDE8, 1 hit
PF00233 PDEase_I, 1 hit
PRINTSiPR00387 PDIESTERASE1
SMARTiView protein in SMART
SM00471 HDc, 1 hit
SM00091 PAS, 1 hit
SUPFAMiSSF55785 SSF55785, 1 hit
TIGRFAMsiTIGR00229 sensory_box, 1 hit
PROSITEiView protein in PROSITE
PS50112 PAS, 1 hit
PS00126 PDEASE_I_1, 1 hit
PS51845 PDEASE_I_2, 1 hit

Sequencei

Sequence statusi: Complete.

E9Q4S1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCAPSIHVS QSGVIYCRDS DESNSPRQTS SVSQGPTAPL HGLFVQTDAA
60 70 80 90 100
DAMPPSRAAG PPGAVRVRRS RAELGSGSST GSSGPATTTC RGRRRHCCSS
110 120 130 140 150
AEAETQTSYT SVKVLLIFAK EDSQSDGFWW ACDRAGYRCN IARTPESALE
160 170 180 190 200
CFLDKHHEII VIDHRQSRNF DAEAVCRSIR ATNPSEHTVI LAVVSQASDD
210 220 230 240 250
HEEASVLPLL HAGFNRRFME NSSIIACYNE LIQIEHGEVR SQFKLRACNS
260 270 280 290 300
VFTALDHCHE AIEITSDDHV IQYVNPAFER MMGYHKGELL GKELADLPKS
310 320 330 340 350
DKNRADLLDT INTCIKKGKE WQGVYYARRK SGDSIQQHVK ITPVIGQGGK
360 370 380 390 400
IRHFVSLKKL CCTTDSNKQI HRIHRDSGDN SQTEPHSFRH KSRRKESIDV
410 420 430 440 450
KSISSRGSDA PSLQNRRYPS MARIHSMTIE APITKVINII NAAQENSPVT
460 470 480 490 500
VAEALDRVLE ILRTTELYSP QLGTKDEDPH TSDLVGGLMT DGLRRLSGNE
510 520 530 540 550
YVFTKNVHHS HSHLSMPITI NDVPPSIAQL LDNEESWDFN IFELEAVTHK
560 570 580 590 600
RPLVYLGLKV FSRFGVCEFL NCTETTLRAW LQVIEANYHS SNAYHNSTHA
610 620 630 640 650
ADVLHATAFF LGKERVKGSL DQLDEVAALI AATVHDVDHP GRTNSFLCNA
660 670 680 690 700
GSELAVLYND TAVLESHHTA LAFQLTVKDT KCNIFKNIDR NHYRTLRQAI
710 720 730 740 750
IDMVLATEMT KHFEHVNKFV NSINKPLAAE SEGSDCECNP TGKNFPENQI
760 770 780 790 800
LIKRMMIKCA DVANPCRPLD LCIEWAGRIS EEYFAQTDEE KRQGLPVVMP
810 820 830 840 850
VFDRNTCSIP KSQISFIDYF ITDMFDAWDA FAHLPALMQH LADNYKHWKT
860
LDDLKCKTLR LPSDS
Length:865
Mass (Da):96,739
Last modified:April 5, 2011 - v1
Checksum:iD1ED48083F1DE072
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC123034 Genomic DNA No translation available.
AC134439 Genomic DNA No translation available.
AC147108 Genomic DNA No translation available.
UniGeneiMm.100167

Genome annotation databases

EnsembliENSMUST00000067082; ENSMUSP00000070465; ENSMUSG00000021684

Similar proteinsi

Entry informationi

Entry nameiPDE8B_MOUSE
AccessioniPrimary (citable) accession number: E9Q4S1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: April 5, 2011
Last modified: May 23, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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