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E9Q4S1 (PDE8B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B

Short name=PDE8B
EC=3.1.4.53
Alternative name(s):
Cell proliferation-inducing gene 22 protein
Gene names
Name:Pde8b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length865 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland By similarity.

Catalytic activity

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Pathway

Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

Tissue specificity

Widely expressed. Ref.2

Developmental stage

Detectable in the adrenal gland of newborn animals. Ref.2

Domain

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.

Contains 1 PAS (PER-ARNT-SIM) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 865865High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
PRO_0000414225

Regions

Domain247 – 31872PAS
Region570 – 848279Catalytic By similarity

Sites

Active site5751Proton donor By similarity
Metal binding5991Divalent metal cation 1 By similarity
Metal binding6351Divalent metal cation 1 By similarity
Metal binding6361Divalent metal cation 1 By similarity
Metal binding6361Divalent metal cation 2 By similarity
Metal binding7611Divalent metal cation 1 By similarity

Amino acid modifications

Modified residue4971Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
E9Q4S1 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: D1ED48083F1DE072

FASTA86596,739
        10         20         30         40         50         60 
MGCAPSIHVS QSGVIYCRDS DESNSPRQTS SVSQGPTAPL HGLFVQTDAA DAMPPSRAAG 

        70         80         90        100        110        120 
PPGAVRVRRS RAELGSGSST GSSGPATTTC RGRRRHCCSS AEAETQTSYT SVKVLLIFAK 

       130        140        150        160        170        180 
EDSQSDGFWW ACDRAGYRCN IARTPESALE CFLDKHHEII VIDHRQSRNF DAEAVCRSIR 

       190        200        210        220        230        240 
ATNPSEHTVI LAVVSQASDD HEEASVLPLL HAGFNRRFME NSSIIACYNE LIQIEHGEVR 

       250        260        270        280        290        300 
SQFKLRACNS VFTALDHCHE AIEITSDDHV IQYVNPAFER MMGYHKGELL GKELADLPKS 

       310        320        330        340        350        360 
DKNRADLLDT INTCIKKGKE WQGVYYARRK SGDSIQQHVK ITPVIGQGGK IRHFVSLKKL 

       370        380        390        400        410        420 
CCTTDSNKQI HRIHRDSGDN SQTEPHSFRH KSRRKESIDV KSISSRGSDA PSLQNRRYPS 

       430        440        450        460        470        480 
MARIHSMTIE APITKVINII NAAQENSPVT VAEALDRVLE ILRTTELYSP QLGTKDEDPH 

       490        500        510        520        530        540 
TSDLVGGLMT DGLRRLSGNE YVFTKNVHHS HSHLSMPITI NDVPPSIAQL LDNEESWDFN 

       550        560        570        580        590        600 
IFELEAVTHK RPLVYLGLKV FSRFGVCEFL NCTETTLRAW LQVIEANYHS SNAYHNSTHA 

       610        620        630        640        650        660 
ADVLHATAFF LGKERVKGSL DQLDEVAALI AATVHDVDHP GRTNSFLCNA GSELAVLYND 

       670        680        690        700        710        720 
TAVLESHHTA LAFQLTVKDT KCNIFKNIDR NHYRTLRQAI IDMVLATEMT KHFEHVNKFV 

       730        740        750        760        770        780 
NSINKPLAAE SEGSDCECNP TGKNFPENQI LIKRMMIKCA DVANPCRPLD LCIEWAGRIS 

       790        800        810        820        830        840 
EEYFAQTDEE KRQGLPVVMP VFDRNTCSIP KSQISFIDYF ITDMFDAWDA FAHLPALMQH 

       850        860 
LADNYKHWKT LDDLKCKTLR LPSDS 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"A cAMP-specific phosphodiesterase (PDE8B) that is mutated in adrenal hyperplasia is expressed widely in human and mouse tissues: a novel PDE8B isoform in human adrenal cortex."
Horvath A., Giatzakis C., Tsang K., Greene E., Osorio P., Boikos S., Libe R., Patronas Y., Robinson-White A., Remmers E., Bertherat J., Nesterova M., Stratakis C.A.
Eur. J. Hum. Genet. 16:1245-1253(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC123034 Genomic DNA. No translation available.
AC134439 Genomic DNA. No translation available.
AC147108 Genomic DNA. No translation available.
UniGeneMm.100167.

3D structure databases

ProteinModelPortalE9Q4S1.
SMRE9Q4S1. Positions 521-854.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEE9Q4S1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000067082; ENSMUSP00000070465; ENSMUSG00000021684.

Organism-specific databases

MGIMGI:2443999. Pde8b.

Phylogenomic databases

GeneTreeENSGT00730000110321.
OMADHRQTQN.

Enzyme and pathway databases

UniPathwayUPA00762; UER00747.

Gene expression databases

BgeeE9Q4S1.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
InterProIPR003607. HD/PDEase_dom.
IPR000014. PAS.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013938. PDEase_PDE8.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamPF13426. PAS_9. 1 hit.
PF08629. PDE8. 1 hit.
PF00233. PDEase_I. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00471. HDc. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMSSF55785. SSF55785. 1 hit.
TIGRFAMsTIGR00229. sensory_box. 1 hit.
PROSITEPS50112. PAS. 1 hit.
PS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROE9Q4S1.
SOURCESearch...

Entry information

Entry namePDE8B_MOUSE
AccessionPrimary (citable) accession number: E9Q4S1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: April 5, 2011
Last modified: June 11, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot