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E9Q4S1

- PDE8B_MOUSE

UniProt

E9Q4S1 - PDE8B_MOUSE

Protein

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B

Gene

Pde8b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (05 Apr 2011)
      Previous versions | rss
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    Functioni

    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland By similarity.By similarity

    Catalytic activityi

    Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei575 – 5751Proton donorBy similarity
    Metal bindingi599 – 5991Divalent metal cation 1By similarity
    Metal bindingi635 – 6351Divalent metal cation 1By similarity
    Metal bindingi636 – 6361Divalent metal cation 1By similarity
    Metal bindingi636 – 6361Divalent metal cation 2By similarity
    Metal bindingi761 – 7611Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. phosphorelay response regulator activity Source: InterPro

    GO - Biological processi

    1. behavioral fear response Source: MGI
    2. cAMP catabolic process Source: UniProtKB-UniPathway
    3. negative regulation of steroid hormone biosynthetic process Source: MGI
    4. neuromuscular process controlling balance Source: MGI
    5. operant conditioning Source: MGI
    6. visual learning Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00762; UER00747.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B (EC:3.1.4.53)
    Short name:
    PDE8B
    Alternative name(s):
    Cell proliferation-inducing gene 22 protein
    Gene namesi
    Name:Pde8b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:2443999. Pde8b.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 865865High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8BPRO_0000414225Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei497 – 4971PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiE9Q4S1.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Developmental stagei

    Detectable in the adrenal gland of newborn animals.1 Publication

    Gene expression databases

    BgeeiE9Q4S1.

    Structurei

    3D structure databases

    ProteinModelPortaliE9Q4S1.
    SMRiE9Q4S1. Positions 521-854.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini247 – 31872PASPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni570 – 848279CatalyticBy similarityAdd
    BLAST

    Domaini

    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

    Sequence similaritiesi

    Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    GeneTreeiENSGT00730000110321.
    OMAiDHRQTQN.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    InterProiIPR003607. HD/PDEase_dom.
    IPR000014. PAS.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    IPR013938. PDEase_PDE8.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view]
    PfamiPF13426. PAS_9. 1 hit.
    PF08629. PDE8. 1 hit.
    PF00233. PDEase_I. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00471. HDc. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF55785. SSF55785. 1 hit.
    TIGRFAMsiTIGR00229. sensory_box. 1 hit.
    PROSITEiPS50112. PAS. 1 hit.
    PS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E9Q4S1-1 [UniParc]FASTAAdd to Basket

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    MGCAPSIHVS QSGVIYCRDS DESNSPRQTS SVSQGPTAPL HGLFVQTDAA    50
    DAMPPSRAAG PPGAVRVRRS RAELGSGSST GSSGPATTTC RGRRRHCCSS 100
    AEAETQTSYT SVKVLLIFAK EDSQSDGFWW ACDRAGYRCN IARTPESALE 150
    CFLDKHHEII VIDHRQSRNF DAEAVCRSIR ATNPSEHTVI LAVVSQASDD 200
    HEEASVLPLL HAGFNRRFME NSSIIACYNE LIQIEHGEVR SQFKLRACNS 250
    VFTALDHCHE AIEITSDDHV IQYVNPAFER MMGYHKGELL GKELADLPKS 300
    DKNRADLLDT INTCIKKGKE WQGVYYARRK SGDSIQQHVK ITPVIGQGGK 350
    IRHFVSLKKL CCTTDSNKQI HRIHRDSGDN SQTEPHSFRH KSRRKESIDV 400
    KSISSRGSDA PSLQNRRYPS MARIHSMTIE APITKVINII NAAQENSPVT 450
    VAEALDRVLE ILRTTELYSP QLGTKDEDPH TSDLVGGLMT DGLRRLSGNE 500
    YVFTKNVHHS HSHLSMPITI NDVPPSIAQL LDNEESWDFN IFELEAVTHK 550
    RPLVYLGLKV FSRFGVCEFL NCTETTLRAW LQVIEANYHS SNAYHNSTHA 600
    ADVLHATAFF LGKERVKGSL DQLDEVAALI AATVHDVDHP GRTNSFLCNA 650
    GSELAVLYND TAVLESHHTA LAFQLTVKDT KCNIFKNIDR NHYRTLRQAI 700
    IDMVLATEMT KHFEHVNKFV NSINKPLAAE SEGSDCECNP TGKNFPENQI 750
    LIKRMMIKCA DVANPCRPLD LCIEWAGRIS EEYFAQTDEE KRQGLPVVMP 800
    VFDRNTCSIP KSQISFIDYF ITDMFDAWDA FAHLPALMQH LADNYKHWKT 850
    LDDLKCKTLR LPSDS 865
    Length:865
    Mass (Da):96,739
    Last modified:April 5, 2011 - v1
    Checksum:iD1ED48083F1DE072
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC123034 Genomic DNA. No translation available.
    AC134439 Genomic DNA. No translation available.
    AC147108 Genomic DNA. No translation available.
    UniGeneiMm.100167.

    Genome annotation databases

    EnsembliENSMUST00000067082; ENSMUSP00000070465; ENSMUSG00000021684.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC123034 Genomic DNA. No translation available.
    AC134439 Genomic DNA. No translation available.
    AC147108 Genomic DNA. No translation available.
    UniGenei Mm.100167.

    3D structure databases

    ProteinModelPortali E9Q4S1.
    SMRi E9Q4S1. Positions 521-854.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi E9Q4S1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000067082 ; ENSMUSP00000070465 ; ENSMUSG00000021684 .

    Organism-specific databases

    MGIi MGI:2443999. Pde8b.

    Phylogenomic databases

    GeneTreei ENSGT00730000110321.
    OMAi DHRQTQN.

    Enzyme and pathway databases

    UniPathwayi UPA00762 ; UER00747 .

    Miscellaneous databases

    PROi E9Q4S1.
    SOURCEi Search...

    Gene expression databases

    Bgeei E9Q4S1.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    InterProi IPR003607. HD/PDEase_dom.
    IPR000014. PAS.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    IPR013938. PDEase_PDE8.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view ]
    Pfami PF13426. PAS_9. 1 hit.
    PF08629. PDE8. 1 hit.
    PF00233. PDEase_I. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00471. HDc. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55785. SSF55785. 1 hit.
    TIGRFAMsi TIGR00229. sensory_box. 1 hit.
    PROSITEi PS50112. PAS. 1 hit.
    PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "A cAMP-specific phosphodiesterase (PDE8B) that is mutated in adrenal hyperplasia is expressed widely in human and mouse tissues: a novel PDE8B isoform in human adrenal cortex."
      Horvath A., Giatzakis C., Tsang K., Greene E., Osorio P., Boikos S., Libe R., Patronas Y., Robinson-White A., Remmers E., Bertherat J., Nesterova M., Stratakis C.A.
      Eur. J. Hum. Genet. 16:1245-1253(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPDE8B_MOUSE
    AccessioniPrimary (citable) accession number: E9Q4S1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 14, 2011
    Last sequence update: April 5, 2011
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3