E9Q4S1 (PDE8B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
Short name=PDE8B
EC=3.1.4.17

Alternative name(s):
Cell proliferation-inducing gene 22 protein

Gene names

Name:

Pde8b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	865 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland By similarity.
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Tissue specificity	Widely expressed. Ref.2
Developmental stage	Detectable in the adrenal gland of newborn animals. Ref.2
Domain	Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Ligand	Metal-binding cAMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cAMP catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway intracellular signal transduction Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Molecular_function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 865	865	High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B		PRO_0000414225
Regions
	Domain	247 – 318	72	PAS
	Region	570 – 848	279	Catalytic By similarity
Sites
	Active site	575	1	Proton donor By similarity
	Metal binding	599	1	Divalent metal cation 1 By similarity
	Metal binding	635	1	Divalent metal cation 1 By similarity
	Metal binding	636	1	Divalent metal cation 1 By similarity
	Metal binding	636	1	Divalent metal cation 2 By similarity
	Metal binding	761	1	Divalent metal cation 1 By similarity
Amino acid modifications
	Modified residue	497	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	E9Q4S1 [UniParc]. Last modified April 5, 2011. Version 1. Checksum: D1ED48083F1DE072	FASTA	865	96,739
	10 20 30 40 50 60 MGCAPSIHVS QSGVIYCRDS DESNSPRQTS SVSQGPTAPL HGLFVQTDAA DAMPPSRAAG 70 80 90 100 110 120 PPGAVRVRRS RAELGSGSST GSSGPATTTC RGRRRHCCSS AEAETQTSYT SVKVLLIFAK 130 140 150 160 170 180 EDSQSDGFWW ACDRAGYRCN IARTPESALE CFLDKHHEII VIDHRQSRNF DAEAVCRSIR 190 200 210 220 230 240 ATNPSEHTVI LAVVSQASDD HEEASVLPLL HAGFNRRFME NSSIIACYNE LIQIEHGEVR 250 260 270 280 290 300 SQFKLRACNS VFTALDHCHE AIEITSDDHV IQYVNPAFER MMGYHKGELL GKELADLPKS 310 320 330 340 350 360 DKNRADLLDT INTCIKKGKE WQGVYYARRK SGDSIQQHVK ITPVIGQGGK IRHFVSLKKL 370 380 390 400 410 420 CCTTDSNKQI HRIHRDSGDN SQTEPHSFRH KSRRKESIDV KSISSRGSDA PSLQNRRYPS 430 440 450 460 470 480 MARIHSMTIE APITKVINII NAAQENSPVT VAEALDRVLE ILRTTELYSP QLGTKDEDPH 490 500 510 520 530 540 TSDLVGGLMT DGLRRLSGNE YVFTKNVHHS HSHLSMPITI NDVPPSIAQL LDNEESWDFN 550 560 570 580 590 600 IFELEAVTHK RPLVYLGLKV FSRFGVCEFL NCTETTLRAW LQVIEANYHS SNAYHNSTHA 610 620 630 640 650 660 ADVLHATAFF LGKERVKGSL DQLDEVAALI AATVHDVDHP GRTNSFLCNA GSELAVLYND 670 680 690 700 710 720 TAVLESHHTA LAFQLTVKDT KCNIFKNIDR NHYRTLRQAI IDMVLATEMT KHFEHVNKFV 730 740 750 760 770 780 NSINKPLAAE SEGSDCECNP TGKNFPENQI LIKRMMIKCA DVANPCRPLD LCIEWAGRIS 790 800 810 820 830 840 EEYFAQTDEE KRQGLPVVMP VFDRNTCSIP KSQISFIDYF ITDMFDAWDA FAHLPALMQH 850 860 LADNYKHWKT LDDLKCKTLR LPSDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"A cAMP-specific phosphodiesterase (PDE8B) that is mutated in adrenal hyperplasia is expressed widely in human and mouse tissues: a novel PDE8B isoform in human adrenal cortex." Horvath A., Giatzakis C., Tsang K., Greene E., Osorio P., Boikos S., Libe R., Patronas Y., Robinson-White A., Remmers E., Bertherat J., Nesterova M., Stratakis C.A. Eur. J. Hum. Genet. 16:1245-1253(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC123034 Genomic DNA. No translation available. AC134439 Genomic DNA. No translation available. AC147108 Genomic DNA. No translation available.
IPI	IPI00261641.
UniGene	Mm.100167.
3D structure databases
ProteinModelPortal	E9Q4S1.
SMR	E9Q4S1. Positions 251-362, 521-854.
ModBase	Search...
Proteomic databases
PRIDE	E9Q4S1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000067082; ENSMUSP00000070465; ENSMUSG00000021684.
Organism-specific databases
MGI	MGI:2443999. Pde8b.
Phylogenomic databases
GeneTree	ENSGT00690000101749.
OMA	LCCTTDN.
Enzyme and pathway databases
UniPathway	UPA00762; UER00747.
Gene expression databases
ArrayExpress	E9Q4S1.
Bgee	E9Q4S1.
Family and domain databases
Gene3D	1.10.1300.10. 1 hit.
InterPro	IPR003607. HD/PDEase_dom. IPR000014. PAS. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. IPR013938. PDEase_PDE8. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Pfam	PF08629. PDE8. 1 hit. PF00233. PDEase_I. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
SUPFAM	SSF55785. SSF55785. 1 hit.
TIGRFAMs	TIGR00229. sensory_box. 1 hit.
PROSITE	PS50112. PAS. 1 hit. PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

PDE8B_MOUSE

Accession

Primary (citable) accession number: E9Q4S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 14, 2011
Last sequence update:	April 5, 2011
Last modified:	May 29, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program