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E9Q4S1

- PDE8B_MOUSE

UniProt

E9Q4S1 - PDE8B_MOUSE

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Protein
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
Gene
Pde8b
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland By similarity.

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei575 – 5751Proton donor By similarity
Metal bindingi599 – 5991Divalent metal cation 1 By similarity
Metal bindingi635 – 6351Divalent metal cation 1 By similarity
Metal bindingi636 – 6361Divalent metal cation 1 By similarity
Metal bindingi636 – 6361Divalent metal cation 2 By similarity
Metal bindingi761 – 7611Divalent metal cation 1 By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. phosphorelay response regulator activity Source: InterPro

GO - Biological processi

  1. cAMP catabolic process Source: UniProtKB-UniPathway
  2. negative regulation of steroid hormone biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00762; UER00747.

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B (EC:3.1.4.53)
Short name:
PDE8B
Alternative name(s):
Cell proliferation-inducing gene 22 protein
Gene namesi
Name:Pde8b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:2443999. Pde8b.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 865865High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
PRO_0000414225Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei497 – 4971Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiE9Q4S1.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Detectable in the adrenal gland of newborn animals.1 Publication

Gene expression databases

BgeeiE9Q4S1.

Structurei

3D structure databases

ProteinModelPortaliE9Q4S1.
SMRiE9Q4S1. Positions 521-854.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini247 – 31872PAS
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni570 – 848279Catalytic By similarity
Add
BLAST

Domaini

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00730000110321.
OMAiDHRQTQN.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR000014. PAS.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013938. PDEase_PDE8.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF13426. PAS_9. 1 hit.
PF08629. PDE8. 1 hit.
PF00233. PDEase_I. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50112. PAS. 1 hit.
PS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E9Q4S1-1 [UniParc]FASTAAdd to Basket

« Hide

MGCAPSIHVS QSGVIYCRDS DESNSPRQTS SVSQGPTAPL HGLFVQTDAA    50
DAMPPSRAAG PPGAVRVRRS RAELGSGSST GSSGPATTTC RGRRRHCCSS 100
AEAETQTSYT SVKVLLIFAK EDSQSDGFWW ACDRAGYRCN IARTPESALE 150
CFLDKHHEII VIDHRQSRNF DAEAVCRSIR ATNPSEHTVI LAVVSQASDD 200
HEEASVLPLL HAGFNRRFME NSSIIACYNE LIQIEHGEVR SQFKLRACNS 250
VFTALDHCHE AIEITSDDHV IQYVNPAFER MMGYHKGELL GKELADLPKS 300
DKNRADLLDT INTCIKKGKE WQGVYYARRK SGDSIQQHVK ITPVIGQGGK 350
IRHFVSLKKL CCTTDSNKQI HRIHRDSGDN SQTEPHSFRH KSRRKESIDV 400
KSISSRGSDA PSLQNRRYPS MARIHSMTIE APITKVINII NAAQENSPVT 450
VAEALDRVLE ILRTTELYSP QLGTKDEDPH TSDLVGGLMT DGLRRLSGNE 500
YVFTKNVHHS HSHLSMPITI NDVPPSIAQL LDNEESWDFN IFELEAVTHK 550
RPLVYLGLKV FSRFGVCEFL NCTETTLRAW LQVIEANYHS SNAYHNSTHA 600
ADVLHATAFF LGKERVKGSL DQLDEVAALI AATVHDVDHP GRTNSFLCNA 650
GSELAVLYND TAVLESHHTA LAFQLTVKDT KCNIFKNIDR NHYRTLRQAI 700
IDMVLATEMT KHFEHVNKFV NSINKPLAAE SEGSDCECNP TGKNFPENQI 750
LIKRMMIKCA DVANPCRPLD LCIEWAGRIS EEYFAQTDEE KRQGLPVVMP 800
VFDRNTCSIP KSQISFIDYF ITDMFDAWDA FAHLPALMQH LADNYKHWKT 850
LDDLKCKTLR LPSDS 865
Length:865
Mass (Da):96,739
Last modified:April 5, 2011 - v1
Checksum:iD1ED48083F1DE072
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC123034 Genomic DNA. No translation available.
AC134439 Genomic DNA. No translation available.
AC147108 Genomic DNA. No translation available.
UniGeneiMm.100167.

Genome annotation databases

EnsembliENSMUST00000067082; ENSMUSP00000070465; ENSMUSG00000021684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC123034 Genomic DNA. No translation available.
AC134439 Genomic DNA. No translation available.
AC147108 Genomic DNA. No translation available.
UniGenei Mm.100167.

3D structure databases

ProteinModelPortali E9Q4S1.
SMRi E9Q4S1. Positions 521-854.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi E9Q4S1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000067082 ; ENSMUSP00000070465 ; ENSMUSG00000021684 .

Organism-specific databases

MGIi MGI:2443999. Pde8b.

Phylogenomic databases

GeneTreei ENSGT00730000110321.
OMAi DHRQTQN.

Enzyme and pathway databases

UniPathwayi UPA00762 ; UER00747 .

Miscellaneous databases

PROi E9Q4S1.
SOURCEi Search...

Gene expression databases

Bgeei E9Q4S1.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
InterProi IPR003607. HD/PDEase_dom.
IPR000014. PAS.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013938. PDEase_PDE8.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view ]
Pfami PF13426. PAS_9. 1 hit.
PF08629. PDE8. 1 hit.
PF00233. PDEase_I. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view ]
SUPFAMi SSF55785. SSF55785. 1 hit.
TIGRFAMsi TIGR00229. sensory_box. 1 hit.
PROSITEi PS50112. PAS. 1 hit.
PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "A cAMP-specific phosphodiesterase (PDE8B) that is mutated in adrenal hyperplasia is expressed widely in human and mouse tissues: a novel PDE8B isoform in human adrenal cortex."
    Horvath A., Giatzakis C., Tsang K., Greene E., Osorio P., Boikos S., Libe R., Patronas Y., Robinson-White A., Remmers E., Bertherat J., Nesterova M., Stratakis C.A.
    Eur. J. Hum. Genet. 16:1245-1253(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPDE8B_MOUSE
AccessioniPrimary (citable) accession number: E9Q4S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: April 5, 2011
Last modified: June 11, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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