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E9Q4S1

- PDE8B_MOUSE

UniProt

E9Q4S1 - PDE8B_MOUSE

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Protein

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B

Gene

Pde8b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland (By similarity).By similarity

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei575 – 5751Proton donorBy similarity
Metal bindingi599 – 5991Divalent metal cation 1By similarity
Metal bindingi635 – 6351Divalent metal cation 1By similarity
Metal bindingi636 – 6361Divalent metal cation 1By similarity
Metal bindingi636 – 6361Divalent metal cation 2By similarity
Metal bindingi761 – 7611Divalent metal cation 1By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. signal transducer activity Source: InterPro

GO - Biological processi

  1. behavioral fear response Source: MGI
  2. cAMP catabolic process Source: UniProtKB-UniPathway
  3. negative regulation of steroid hormone biosynthetic process Source: MGI
  4. neuromuscular process controlling balance Source: MGI
  5. operant conditioning Source: MGI
  6. phosphorelay signal transduction system Source: InterPro
  7. visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_242871. G alpha (s) signalling events.
UniPathwayiUPA00762; UER00747.

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B (EC:3.1.4.53)
Short name:
PDE8B
Alternative name(s):
Cell proliferation-inducing gene 22 protein
Gene namesi
Name:Pde8b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:2443999. Pde8b.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 865865High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8BPRO_0000414225Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei497 – 4971PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiE9Q4S1.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Detectable in the adrenal gland of newborn animals.1 Publication

Gene expression databases

BgeeiE9Q4S1.
ExpressionAtlasiE9Q4S1. baseline and differential.

Structurei

3D structure databases

ProteinModelPortaliE9Q4S1.
SMRiE9Q4S1. Positions 521-854.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini247 – 31872PASPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni570 – 848279CatalyticBy similarityAdd
BLAST

Domaini

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similaritiesi

Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000118889.
InParanoidiE9Q4S1.
OMAiDHRQTQN.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR000014. PAS.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013938. PDEase_PDE8.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF13426. PAS_9. 1 hit.
PF08629. PDE8. 1 hit.
PF00233. PDEase_I. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50112. PAS. 1 hit.
PS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E9Q4S1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGCAPSIHVS QSGVIYCRDS DESNSPRQTS SVSQGPTAPL HGLFVQTDAA
60 70 80 90 100
DAMPPSRAAG PPGAVRVRRS RAELGSGSST GSSGPATTTC RGRRRHCCSS
110 120 130 140 150
AEAETQTSYT SVKVLLIFAK EDSQSDGFWW ACDRAGYRCN IARTPESALE
160 170 180 190 200
CFLDKHHEII VIDHRQSRNF DAEAVCRSIR ATNPSEHTVI LAVVSQASDD
210 220 230 240 250
HEEASVLPLL HAGFNRRFME NSSIIACYNE LIQIEHGEVR SQFKLRACNS
260 270 280 290 300
VFTALDHCHE AIEITSDDHV IQYVNPAFER MMGYHKGELL GKELADLPKS
310 320 330 340 350
DKNRADLLDT INTCIKKGKE WQGVYYARRK SGDSIQQHVK ITPVIGQGGK
360 370 380 390 400
IRHFVSLKKL CCTTDSNKQI HRIHRDSGDN SQTEPHSFRH KSRRKESIDV
410 420 430 440 450
KSISSRGSDA PSLQNRRYPS MARIHSMTIE APITKVINII NAAQENSPVT
460 470 480 490 500
VAEALDRVLE ILRTTELYSP QLGTKDEDPH TSDLVGGLMT DGLRRLSGNE
510 520 530 540 550
YVFTKNVHHS HSHLSMPITI NDVPPSIAQL LDNEESWDFN IFELEAVTHK
560 570 580 590 600
RPLVYLGLKV FSRFGVCEFL NCTETTLRAW LQVIEANYHS SNAYHNSTHA
610 620 630 640 650
ADVLHATAFF LGKERVKGSL DQLDEVAALI AATVHDVDHP GRTNSFLCNA
660 670 680 690 700
GSELAVLYND TAVLESHHTA LAFQLTVKDT KCNIFKNIDR NHYRTLRQAI
710 720 730 740 750
IDMVLATEMT KHFEHVNKFV NSINKPLAAE SEGSDCECNP TGKNFPENQI
760 770 780 790 800
LIKRMMIKCA DVANPCRPLD LCIEWAGRIS EEYFAQTDEE KRQGLPVVMP
810 820 830 840 850
VFDRNTCSIP KSQISFIDYF ITDMFDAWDA FAHLPALMQH LADNYKHWKT
860
LDDLKCKTLR LPSDS
Length:865
Mass (Da):96,739
Last modified:April 5, 2011 - v1
Checksum:iD1ED48083F1DE072
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC123034 Genomic DNA. No translation available.
AC134439 Genomic DNA. No translation available.
AC147108 Genomic DNA. No translation available.
UniGeneiMm.100167.

Genome annotation databases

EnsembliENSMUST00000067082; ENSMUSP00000070465; ENSMUSG00000021684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC123034 Genomic DNA. No translation available.
AC134439 Genomic DNA. No translation available.
AC147108 Genomic DNA. No translation available.
UniGenei Mm.100167.

3D structure databases

ProteinModelPortali E9Q4S1.
SMRi E9Q4S1. Positions 521-854.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi E9Q4S1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000067082 ; ENSMUSP00000070465 ; ENSMUSG00000021684 .

Organism-specific databases

MGIi MGI:2443999. Pde8b.

Phylogenomic databases

GeneTreei ENSGT00760000118889.
InParanoidi E9Q4S1.
OMAi DHRQTQN.

Enzyme and pathway databases

UniPathwayi UPA00762 ; UER00747 .
Reactomei REACT_242871. G alpha (s) signalling events.

Miscellaneous databases

PROi E9Q4S1.
SOURCEi Search...

Gene expression databases

Bgeei E9Q4S1.
ExpressionAtlasi E9Q4S1. baseline and differential.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
InterProi IPR003607. HD/PDEase_dom.
IPR000014. PAS.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013938. PDEase_PDE8.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view ]
Pfami PF13426. PAS_9. 1 hit.
PF08629. PDE8. 1 hit.
PF00233. PDEase_I. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view ]
SUPFAMi SSF55785. SSF55785. 1 hit.
TIGRFAMsi TIGR00229. sensory_box. 1 hit.
PROSITEi PS50112. PAS. 1 hit.
PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "A cAMP-specific phosphodiesterase (PDE8B) that is mutated in adrenal hyperplasia is expressed widely in human and mouse tissues: a novel PDE8B isoform in human adrenal cortex."
    Horvath A., Giatzakis C., Tsang K., Greene E., Osorio P., Boikos S., Libe R., Patronas Y., Robinson-White A., Remmers E., Bertherat J., Nesterova M., Stratakis C.A.
    Eur. J. Hum. Genet. 16:1245-1253(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPDE8B_MOUSE
AccessioniPrimary (citable) accession number: E9Q4S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: April 5, 2011
Last modified: November 26, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3