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Protein

PR domain zinc finger protein 14

Gene

Prdm14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that has both positive and negative roles on transcription (By similarity). Plays a role in cellular pluripotency. Essential for germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei355S-adenosyl-L-methioninePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri390 – 416C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST27
Zinc fingeri422 – 445C2H2-type 2PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri451 – 473C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri479 – 501C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri507 – 530C2H2-type 5PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri536 – 558C2H2-type 6PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

  • chromatin DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • methyltransferase activity Source: UniProtKB-KW
  • RNA binding Source: MGI

GO - Biological processi

  • cell fate specification Source: MGI
  • cell morphogenesis Source: MGI
  • embryo implantation Source: MGI
  • fertilization Source: MGI
  • germ cell development Source: UniProtKB
  • germ-line stem cell population maintenance Source: MGI
  • histone H3-R26 methylation Source: MGI
  • homeostasis of number of cells within a tissue Source: MGI
  • inactivation of paternal X chromosome Source: MGI
  • inner cell mass cell fate commitment Source: MGI
  • negative regulation of fibroblast growth factor receptor signaling pathway Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of flagellated sperm motility Source: UniProtKB
  • positive regulation of stem cell population maintenance Source: UniProtKB
  • regulation of DNA methylation Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of gene expression, epigenetic Source: MGI
  • stem cell population maintenance Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, S-adenosyl-L-methionine, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
PR domain zinc finger protein 14 (EC:2.1.1.-)
Alternative name(s):
PR domain-containing protein 14
Gene namesi
Name:Prdm14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:3588194. Prdm14.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant mice are born with an expected Mendelian ratio and looked grossly normal. However, both females and males are sterile, ovaries and testes being completely devoid of germ cells.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi339Y → R: Impairs interaction with CBFA2T2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004176661 – 561PR domain zinc finger protein 14Add BLAST561

Proteomic databases

PaxDbiE9Q3T6.
PRIDEiE9Q3T6.

PTM databases

PhosphoSitePlusiE9Q3T6.

Expressioni

Tissue specificityi

Restricted to embryonic stem cells and primordial germ cells. Not detected in epiblast-derived stem cells.2 Publications

Developmental stagei

At 3.5 dpc, weak and transient expression in the inner cell mass cells of blastocysts. This expression disappears by 5.5 dpc. Expression starts again in committed PGCs around 6.5 dpc in the extraembryonic mesoderm contiguous from the most proximal epiblast (at protein level). Expression persists specifically in PGCs until about 13.5-14.5 dpc both in females and males.1 Publication

Gene expression databases

BgeeiENSMUSG00000042414.
GenevisibleiE9Q3T6. MM.

Interactioni

Subunit structurei

Interacts with CBFA2T2.2 Publications

Protein-protein interaction databases

BioGridi238771. 1 interactor.
DIPiDIP-62031N.
STRINGi10090.ENSMUSP00000044245.

Structurei

Secondary structure

1561
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi189 – 197Combined sources9
Beta strandi199 – 201Combined sources3
Beta strandi203 – 205Combined sources3
Beta strandi207 – 209Combined sources3
Beta strandi243 – 250Combined sources8
Beta strandi253 – 262Combined sources10
Beta strandi276 – 279Combined sources4
Beta strandi292 – 297Combined sources6
Beta strandi300 – 306Combined sources7
Helixi313 – 316Combined sources4
Helixi323 – 325Combined sources3
Beta strandi328 – 333Combined sources6
Beta strandi336 – 343Combined sources8
Beta strandi352 – 355Combined sources4
Beta strandi359 – 362Combined sources4
Beta strandi365 – 368Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5ECJX-ray3.05A/B184-373[»]
ProteinModelPortaliE9Q3T6.
SMRiE9Q3T6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini241 – 356SETPROSITE-ProRule annotationAdd BLAST116

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 373Interaction with CBFA2T22 PublicationsAdd BLAST190

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily.PROSITE-ProRule annotation
Contains 6 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri390 – 416C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST27
Zinc fingeri422 – 445C2H2-type 2PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri451 – 473C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri479 – 501C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri507 – 530C2H2-type 5PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri536 – 558C2H2-type 6PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00830000128284.
HOGENOMiHOG000115684.
InParanoidiE9Q3T6.
OMAiFAFRMAP.
OrthoDBiEOG091G0EWM.
TreeFamiTF106412.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR001214. SET_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00856. SET. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00317. SET. 1 hit.
SM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS50280. SET. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E9Q3T6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPPSGETQ SQDKANYLPQ SNPHHLTTYY AHAPGYSHFR NLATTEEEFQ
60 70 80 90 100
PWKLAAAVLE SQAMAPLDAF RMTAPLLNPG LAVQSEPLYN LPWYKLSPWN
110 120 130 140 150
RIPQFTPEVP RFLDSTEHRS SGSSNQNLVL GGGGGQISGQ RWEAENLLLP
160 170 180 190 200
SPVIASLLPD GIKSSQSISV PQTLNQEGKL PFCGFNFTEE ELSFVLYGAI
210 220 230 240 250
ASPEHPTDLQ HAISGILVPT ESSGSNHLHK TLDKDSLQLP EGLCLMQTSF
260 270 280 290 300
GDVPHFGVFC SDFIAKGVRF GPFRGRVVNA SEVKAHRDNS RMWEIFEDGH
310 320 330 340 350
LSHFIDGKGS GNWMSYVNCA RFPKEQNLLA VQHQGQIFYE SCRDIQRNQE
360 370 380 390 400
LLVWYGNGYE KFLGVPMNLR VTEQGGQQLS ESSEESAEGY RCERCGKVFT
410 420 430 440 450
YKYYRDKHLK YTPCVDKGDR KFPCSLCQRS FEKRDRLRIH ILHVHERHRP
460 470 480 490 500
YLCSTCGKSF SQSSSLNKHM RVHSGDRPYQ CVYCTKKFTA SSILRTHIRQ
510 520 530 540 550
HSGEKPFKCK HCGKAFASHA AHDSHVRRSH KDNGRSSCDI CGKGFLDQEA
560
FYAHMRLHKT C
Length:561
Mass (Da):63,374
Last modified:April 5, 2011 - v1
Checksum:i03D83B5365C9FBAB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50Q → E in BAE24596 (PubMed:19468303).Curated1
Sequence conflicti326Q → L in BAE24596 (PubMed:19468303).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC091248 Genomic DNA. No translation available.
AK141218 mRNA. Translation: BAE24596.1.
CCDSiCCDS48221.1.
RefSeqiNP_001074678.2. NM_001081209.2.
UniGeneiMm.309380.

Genome annotation databases

EnsembliENSMUST00000047577; ENSMUSP00000044245; ENSMUSG00000042414.
GeneIDi383491.
KEGGimmu:383491.
UCSCiuc007aik.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC091248 Genomic DNA. No translation available.
AK141218 mRNA. Translation: BAE24596.1.
CCDSiCCDS48221.1.
RefSeqiNP_001074678.2. NM_001081209.2.
UniGeneiMm.309380.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5ECJX-ray3.05A/B184-373[»]
ProteinModelPortaliE9Q3T6.
SMRiE9Q3T6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi238771. 1 interactor.
DIPiDIP-62031N.
STRINGi10090.ENSMUSP00000044245.

PTM databases

PhosphoSitePlusiE9Q3T6.

Proteomic databases

PaxDbiE9Q3T6.
PRIDEiE9Q3T6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047577; ENSMUSP00000044245; ENSMUSG00000042414.
GeneIDi383491.
KEGGimmu:383491.
UCSCiuc007aik.1. mouse.

Organism-specific databases

CTDi63978.
MGIiMGI:3588194. Prdm14.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00830000128284.
HOGENOMiHOG000115684.
InParanoidiE9Q3T6.
OMAiFAFRMAP.
OrthoDBiEOG091G0EWM.
TreeFamiTF106412.

Miscellaneous databases

PROiE9Q3T6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042414.
GenevisibleiE9Q3T6. MM.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR001214. SET_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00856. SET. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00317. SET. 1 hit.
SM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS50280. SET. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRD14_MOUSE
AccessioniPrimary (citable) accession number: E9Q3T6
Secondary accession number(s): Q3URU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: April 5, 2011
Last modified: November 2, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.