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Protein

PR domain zinc finger protein 14

Gene

Prdm14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that has both positive and negative roles on transcription. Plays a role in cellular pluripotency (By similarity). Essential for germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei355 – 3551S-adenosyl-L-methioninePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri390 – 41627C2H2-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri422 – 44524C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri451 – 47323C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri479 – 50123C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri507 – 53024C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri536 – 55823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • chromatin DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • methyltransferase activity Source: UniProtKB-KW
  • RNA binding Source: MGI

GO - Biological processi

  • cell fate specification Source: MGI
  • cell morphogenesis Source: MGI
  • embryo implantation Source: MGI
  • fertilization Source: MGI
  • germ cell development Source: MGI
  • germ-line stem cell population maintenance Source: MGI
  • histone H3-R26 methylation Source: MGI
  • homeostasis of number of cells within a tissue Source: MGI
  • inactivation of paternal X chromosome Source: MGI
  • inner cell mass cell fate commitment Source: MGI
  • negative regulation of fibroblast growth factor receptor signaling pathway Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of DNA methylation Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of gene expression, epigenetic Source: MGI
  • stem cell population maintenance Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, S-adenosyl-L-methionine, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
PR domain zinc finger protein 14 (EC:2.1.1.-)
Alternative name(s):
PR domain-containing protein 14
Gene namesi
Name:Prdm14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:3588194. Prdm14.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant mice are born with an expected Mendelian ratio and looked grossly normal. However, both females and males are sterile, ovaries and testes being completely devoid of germ cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561PR domain zinc finger protein 14PRO_0000417666Add
BLAST

Proteomic databases

PaxDbiE9Q3T6.
PRIDEiE9Q3T6.

PTM databases

PhosphoSiteiE9Q3T6.

Expressioni

Tissue specificityi

Restricted to embryonic stem cells and primordial germ cells. Not detcted in epiblast-derived stem cells.2 Publications

Developmental stagei

At 3.5 dpc, weak and transient expression in the inner cell mass cells of blastocysts. This expression disappears by 5.5 dpc. Expression starts again in committed PGCs around 6.5 dpc in the extraembryonic mesoderm contiguous from the most proximal epiblast (at protein level). Expression persists specifically in PGCs until about 13.5-14.5 dpc both in females and males.1 Publication

Gene expression databases

BgeeiENSMUSG00000042414.
GenevisibleiE9Q3T6. MM.

Interactioni

Protein-protein interaction databases

BioGridi238771. 1 interaction.
STRINGi10090.ENSMUSP00000044245.

Structurei

Secondary structure

1
561
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi189 – 1979Combined sources
Beta strandi199 – 2013Combined sources
Beta strandi203 – 2053Combined sources
Beta strandi207 – 2093Combined sources
Beta strandi243 – 2508Combined sources
Beta strandi253 – 26210Combined sources
Beta strandi276 – 2794Combined sources
Beta strandi292 – 2976Combined sources
Beta strandi300 – 3067Combined sources
Helixi313 – 3164Combined sources
Helixi323 – 3253Combined sources
Beta strandi328 – 3336Combined sources
Beta strandi336 – 3438Combined sources
Beta strandi352 – 3554Combined sources
Beta strandi359 – 3624Combined sources
Beta strandi365 – 3684Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5ECJX-ray3.05A/B184-373[»]
ProteinModelPortaliE9Q3T6.
SMRiE9Q3T6. Positions 184-558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini241 – 356116SETPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily.PROSITE-ProRule annotation
Contains 6 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri390 – 41627C2H2-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri422 – 44524C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri451 – 47323C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri479 – 50123C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri507 – 53024C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri536 – 55823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00830000128284.
HOGENOMiHOG000115684.
InParanoidiE9Q3T6.
OMAiFAFRMAP.
OrthoDBiEOG091G0EWM.
TreeFamiTF106412.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR001214. SET_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00856. SET. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00317. SET. 1 hit.
SM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS50280. SET. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E9Q3T6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPPSGETQ SQDKANYLPQ SNPHHLTTYY AHAPGYSHFR NLATTEEEFQ
60 70 80 90 100
PWKLAAAVLE SQAMAPLDAF RMTAPLLNPG LAVQSEPLYN LPWYKLSPWN
110 120 130 140 150
RIPQFTPEVP RFLDSTEHRS SGSSNQNLVL GGGGGQISGQ RWEAENLLLP
160 170 180 190 200
SPVIASLLPD GIKSSQSISV PQTLNQEGKL PFCGFNFTEE ELSFVLYGAI
210 220 230 240 250
ASPEHPTDLQ HAISGILVPT ESSGSNHLHK TLDKDSLQLP EGLCLMQTSF
260 270 280 290 300
GDVPHFGVFC SDFIAKGVRF GPFRGRVVNA SEVKAHRDNS RMWEIFEDGH
310 320 330 340 350
LSHFIDGKGS GNWMSYVNCA RFPKEQNLLA VQHQGQIFYE SCRDIQRNQE
360 370 380 390 400
LLVWYGNGYE KFLGVPMNLR VTEQGGQQLS ESSEESAEGY RCERCGKVFT
410 420 430 440 450
YKYYRDKHLK YTPCVDKGDR KFPCSLCQRS FEKRDRLRIH ILHVHERHRP
460 470 480 490 500
YLCSTCGKSF SQSSSLNKHM RVHSGDRPYQ CVYCTKKFTA SSILRTHIRQ
510 520 530 540 550
HSGEKPFKCK HCGKAFASHA AHDSHVRRSH KDNGRSSCDI CGKGFLDQEA
560
FYAHMRLHKT C
Length:561
Mass (Da):63,374
Last modified:April 5, 2011 - v1
Checksum:i03D83B5365C9FBAB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501Q → E in BAE24596 (PubMed:19468303).Curated
Sequence conflicti326 – 3261Q → L in BAE24596 (PubMed:19468303).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC091248 Genomic DNA. No translation available.
AK141218 mRNA. Translation: BAE24596.1.
CCDSiCCDS48221.1.
RefSeqiNP_001074678.2. NM_001081209.2.
UniGeneiMm.309380.

Genome annotation databases

EnsembliENSMUST00000047577; ENSMUSP00000044245; ENSMUSG00000042414.
GeneIDi383491.
KEGGimmu:383491.
UCSCiuc007aik.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC091248 Genomic DNA. No translation available.
AK141218 mRNA. Translation: BAE24596.1.
CCDSiCCDS48221.1.
RefSeqiNP_001074678.2. NM_001081209.2.
UniGeneiMm.309380.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5ECJX-ray3.05A/B184-373[»]
ProteinModelPortaliE9Q3T6.
SMRiE9Q3T6. Positions 184-558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi238771. 1 interaction.
STRINGi10090.ENSMUSP00000044245.

PTM databases

PhosphoSiteiE9Q3T6.

Proteomic databases

PaxDbiE9Q3T6.
PRIDEiE9Q3T6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047577; ENSMUSP00000044245; ENSMUSG00000042414.
GeneIDi383491.
KEGGimmu:383491.
UCSCiuc007aik.1. mouse.

Organism-specific databases

CTDi63978.
MGIiMGI:3588194. Prdm14.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00830000128284.
HOGENOMiHOG000115684.
InParanoidiE9Q3T6.
OMAiFAFRMAP.
OrthoDBiEOG091G0EWM.
TreeFamiTF106412.

Miscellaneous databases

PROiE9Q3T6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042414.
GenevisibleiE9Q3T6. MM.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR001214. SET_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00856. SET. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00317. SET. 1 hit.
SM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS50280. SET. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRD14_MOUSE
AccessioniPrimary (citable) accession number: E9Q3T6
Secondary accession number(s): Q3URU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: April 5, 2011
Last modified: September 7, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.