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E9PZM4

- CHD2_MOUSE

UniProt

E9PZM4 - CHD2_MOUSE

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Protein

Chromodomain-helicase-DNA-binding protein 2

Gene

Chd2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

DNA-binding helicase that specifically binds to the promoter of target genes, leading to chromatin remodeling, possibly by promoting deposition of histone H3.3. Involved in myogenesis via interaction with MYOD1: binds to myogenic gene regulatory sequences and mediates incorporation of histone H3.3 prior to the onset of myogenic gene expression, promoting their expression.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi509 – 5168ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. core promoter sequence-specific DNA binding Source: UniProtKB
  3. helicase activity Source: UniProtKB-KW
  4. histone binding Source: UniProtKB
  5. poly(A) RNA binding Source: Ensembl

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: MGI
  2. chromatin modification Source: UniProtKB-KW
  3. hematopoietic stem cell differentiation Source: MGI
  4. muscle organ development Source: UniProtKB
  5. regulation of transcription, DNA-templated Source: UniProtKB-KW
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Helicase, Hydrolase

Keywords - Biological processi

Myogenesis, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chromodomain-helicase-DNA-binding protein 2 (EC:3.6.4.12)
Short name:
CHD-2
Alternative name(s):
ATP-dependent helicase CHD2
Gene namesi
Name:Chd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:2448567. Chd2.

Subcellular locationi

Nucleus 1 Publication
Note: Binds to myogenic gene promoters.

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Growth delay late in embryogenesis and perinatal lethality (PubMed:16810678). Heterozygous mice show decreased neonatal viability. Heterozygous mice display glomerulopathy, proteinuria and impaired kidney function. Glomerulopathy may be associated with anemia (PubMed:19142019). Heterozygous mutant also show increased extramedullary hematopoiesis and susceptibility to lymphomas, with defects in hematopoietic stem cell differentiation (PubMed:19137022).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18271827Chromodomain-helicase-DNA-binding protein 2PRO_0000425206Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei207 – 2071PhosphoserineBy similarity
Modified residuei208 – 2081PhosphoserineBy similarity
Modified residuei240 – 2401PhosphothreonineBy similarity
Modified residuei242 – 2421PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiE9PZM4.
PRIDEiE9PZM4.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiE9PZM4.
ExpressionAtlasiE9PZM4. baseline and differential.

Interactioni

Subunit structurei

Interacts with MYOD1. Interacts with histone H3.3.

Structurei

3D structure databases

ProteinModelPortaliE9PZM4.
SMRiE9PZM4. Positions 261-343, 1129-1328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini261 – 35393Chromo 1PROSITE-ProRule annotationAdd
BLAST
Domaini378 – 45679Chromo 2PROSITE-ProRule annotationAdd
BLAST
Domaini496 – 666171Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini795 – 946152Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi617 – 6204DEAH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi13 – 7563Ser-richAdd
BLAST
Compositional biasi209 – 2157Poly-Asp
Compositional biasi966 – 1066101Glu-richAdd
BLAST
Compositional biasi1303 – 1439137Lys-richAdd
BLAST
Compositional biasi1562 – 15654Poly-Glu
Compositional biasi1596 – 1720125His-richAdd
BLAST

Sequence similaritiesi

Contains 2 chromo domains.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00760000119067.
InParanoidiE9PZM4.
KOiK11367.
OMAiKRPYEQY.
TreeFamiTF313461.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
IPR025260. DUF4208.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00385. Chromo. 2 hits.
PF13907. DUF4208. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 2 hits.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF54160. SSF54160. 2 hits.
PROSITEiPS00598. CHROMO_1. 2 hits.
PS50013. CHROMO_2. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E9PZM4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMRNKDKSQE EDSSLHSNAS SRSASEEVSG SDSGSQSESE QGSEPGSGHG
60 70 80 90 100
SESNSSSESS ESQSESESES AGSKSQPVLP EAKEKPASKK ERIADVKKMW
110 120 130 140 150
EEYPDVYGVR RSNRSRQEPS RFNVKEEASS GSESGSPKRR GQRQLKKQEK
160 170 180 190 200
WKQDPSEDEQ EQGTSAESEA EQKKGKARRP VPRRTVPKPQ VKKQPKIQRG
210 220 230 240 250
KRKKQESSDD DDDDDEAPKR QTRRRAAKNV SYKEDDDFET DSDDLIEMTG
260 270 280 290 300
EGGDEQQDNS ETIEKVLDSR LGKKGATGAS TTVYAVEANG DPSDDFDTER
310 320 330 340 350
EEGEVQYLIK WKGWSYIHST WESEDSLQQQ KVKGLKKLEN FKKKEDEVKQ
360 370 380 390 400
WLGKVSPEDV EYFSCQQELA SELNKQYQIV ERVIAVKTSK STLGQTDFPA
410 420 430 440 450
HSRKPAPSNE PEYLCKWMGL PYSECSWEDE ALIGKKFQNC IDSFHSRNNS
460 470 480 490 500
KTIPTRECKA LKQRPRFVAL KKQPAYLGGE SLELRDYQLE GLNWLAHSWC
510 520 530 540 550
KSNSVILADE MGLGKTIQTI SFLSYLFHQH QLYGPFLIVV PLSTLTSWQR
560 570 580 590 600
EFEIWAPEIN VVVYIGDLMS RNTIREYEWI HSQTKRLKFN ALITTYEILL
610 620 630 640 650
KDKTVLGSIN WAFLGVDEAH RLKNDDSLLY KTLIDFKSNH RLLITGTPLQ
660 670 680 690 700
NSLKELWSLL HFIMPEKFEF WEDFEEDHGK GRENGYQSLH KVLEPFLLRR
710 720 730 740 750
VKKDVEKSLP AKVEQILRVE MSALQKQYYK WILTRNYKAL AKGTRGSTSG
760 770 780 790 800
FLNIVMELKK CCNHCYLIKA PEDSERESGQ EVLQSLIRSS GKLILLDKLL
810 820 830 840 850
TRLRERGNRV LIFSQMVRML DILAEYLTIK HYPFQRLDGS IKGEIRKQAL
860 870 880 890 900
DHFNADGSED FCFLLSTRAG GLGINLASAD TVVIFDSDWN PQNDLQAQAR
910 920 930 940 950
AHRIGQKKQV NIYRLVTKGT VEEEIIERAK KKMVLDHLVI QRMDTTGRTV
960 970 980 990 1000
LENNSGRSNS NPFNKEELTA ILKFGAEDLF KEIEGEESEP QEMDIDEILR
1010 1020 1030 1040 1050
LAETRENEVS TSATDELLSQ FKVANFATME DEEELEERPH KDWDEIIPEE
1060 1070 1080 1090 1100
QRKKVEEEER QKELEEIYML PRIRSSTKKA QTNDSDSDTE SKRQAQRSSA
1110 1120 1130 1140 1150
SESETDDSDD DKKPKRRGRP RSVRKDLVEG FTDAEIRRFI KAYKKFGLPL
1160 1170 1180 1190 1200
ERLECIARDA ELVDKSVADL KRLGELIHNS CVSAMQEYEE QLKESTSEGK
1210 1220 1230 1240 1250
GPGKRRGPTI KISGVQVNVK SIIQHEEEFE MLHKSIPVDP EEKKKYCLTC
1260 1270 1280 1290 1300
RVKAAHFDVE WGVEDDSRLL LGIYEHGYGN WELIKTDPEL KLTDKILPVE
1310 1320 1330 1340 1350
TDKKPQGKQL QTRVDYLLKL LRKGLEKKGT VASGEEAKLK KRKPRVKKEN
1360 1370 1380 1390 1400
KAPRLKDEHG LEPASPRHSD NPSEEGEVKD DGLEKSPTKK KQKKKENKEN
1410 1420 1430 1440 1450
KEKPVSSRKD REGDKERKKS KDKKEKVKGG DGKSSSKSKR SQGPVHITAG
1460 1470 1480 1490 1500
SEPVPIGEDE DDDLDQETFS ICKERMRPVK KALKQLDKPD KGLSVQEQLE
1510 1520 1530 1540 1550
HTRNCLLKIG DRIAECLKAY SDQEHIKLWR RNLWIFVSKF TEFDARKLHK
1560 1570 1580 1590 1600
LYKMAHKKRS QEEEEQKKKD DSLGGKKPFR PEASGSSRDS LISQSHTSHN
1610 1620 1630 1640 1650
LHPQKPHLPA SHGPQMHGHP RDNYSHPNKR HFSNADRGDW QRERKFNYGG
1660 1670 1680 1690 1700
GNSAPWGGDR HHQYEQHWYK DHHYGDRRHM DAHRSGSYRP NNMSRKRPYE
1710 1720 1730 1740 1750
QYNSDRDHRG HRDYYDRHHH DSKRRRSDDF RPQNYHQQDF RRMSDHRPTM
1760 1770 1780 1790 1800
GYHGQGPSDH YRSFHTDKLG EYKQPMPSLH TALSDPRSPP SQKSPHDSKS
1810 1820
PLDHRSPLER SLEQKNNPDY NWNVRKT
Length:1,827
Mass (Da):210,804
Last modified:April 5, 2011 - v1
Checksum:i60B317C82F373453
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC099699 Genomic DNA. No translation available.
AC154883 Genomic DNA. No translation available.
CCDSiCCDS52274.1.
RefSeqiNP_001074814.2. NM_001081345.2.
UniGeneiMm.34539.
Mm.34955.

Genome annotation databases

EnsembliENSMUST00000169922; ENSMUSP00000126352; ENSMUSG00000078671.
GeneIDi244059.
KEGGimmu:244059.
UCSCiuc009hrd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC099699 Genomic DNA. No translation available.
AC154883 Genomic DNA. No translation available.
CCDSi CCDS52274.1.
RefSeqi NP_001074814.2. NM_001081345.2.
UniGenei Mm.34539.
Mm.34955.

3D structure databases

ProteinModelPortali E9PZM4.
SMRi E9PZM4. Positions 261-343, 1129-1328.
ModBasei Search...
MobiDBi Search...

Proteomic databases

MaxQBi E9PZM4.
PRIDEi E9PZM4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000169922 ; ENSMUSP00000126352 ; ENSMUSG00000078671 .
GeneIDi 244059.
KEGGi mmu:244059.
UCSCi uc009hrd.2. mouse.

Organism-specific databases

CTDi 1106.
MGIi MGI:2448567. Chd2.

Phylogenomic databases

GeneTreei ENSGT00760000119067.
InParanoidi E9PZM4.
KOi K11367.
OMAi KRPYEQY.
TreeFami TF313461.

Miscellaneous databases

ChiTaRSi Chd2. mouse.
NextBioi 386098.
PROi E9PZM4.
SOURCEi Search...

Gene expression databases

Bgeei E9PZM4.
ExpressionAtlasi E9PZM4. baseline and differential.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
IPR025260. DUF4208.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view ]
Pfami PF00385. Chromo. 2 hits.
PF13907. DUF4208. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view ]
SMARTi SM00298. CHROMO. 2 hits.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF54160. SSF54160. 2 hits.
PROSITEi PS00598. CHROMO_1. 2 hits.
PS50013. CHROMO_2. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "Mutation of the SNF2 family member Chd2 affects mouse development and survival."
    Marfella C.G., Ohkawa Y., Coles A.H., Garlick D.S., Jones S.N., Imbalzano A.N.
    J. Cell. Physiol. 209:162-171(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  3. Erratum
    Marfella C.G., Ohkawa Y., Coles A.H., Garlick D.S., Jones S.N., Imbalzano A.N.
    J. Cell. Physiol. 212:562-562(2007)
  4. "A mutation in the mouse Chd2 chromatin remodeling enzyme results in a complex renal phenotype."
    Marfella C.G., Henninger N., LeBlanc S.E., Krishnan N., Garlick D.S., Holzman L.B., Imbalzano A.N.
    Kidney Blood Press. Res. 31:421-432(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  5. "Role of chromodomain helicase DNA-binding protein 2 in DNA damage response signaling and tumorigenesis."
    Nagarajan P., Onami T.M., Rajagopalan S., Kania S., Donnell R., Venkatachalam S.
    Oncogene 28:1053-1062(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. Cited for: FUNCTION, SUBCELLULAR LOCATION, DNA-BINDING.

Entry informationi

Entry nameiCHD2_MOUSE
AccessioniPrimary (citable) accession number: E9PZM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: April 5, 2011
Last modified: November 26, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3