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E9PZM4

- CHD2_MOUSE

UniProt

E9PZM4 - CHD2_MOUSE

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Protein
Chromodomain-helicase-DNA-binding protein 2
Gene
Chd2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

DNA-binding helicase that specifically binds to the promoter of target genes, leading to chromatin remodeling, possibly by promoting deposition of histone H3.3. Involved in myogenesis via interaction with MYOD1: binds to myogenic gene regulatory sequences and mediates incorporation of histone H3.3 prior to the onset of myogenic gene expression, promoting their expression.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi509 – 5168ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. core promoter sequence-specific DNA binding Source: UniProtKB
  3. helicase activity Source: UniProtKB-KW
  4. histone binding Source: UniProtKB
  5. protein binding Source: UniProtKB

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: MGI
  2. chromatin modification Source: UniProtKB-KW
  3. hematopoietic stem cell differentiation Source: MGI
  4. muscle organ development Source: UniProtKB
  5. regulation of transcription, DNA-templated Source: UniProtKB-KW
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Helicase, Hydrolase

Keywords - Biological processi

Myogenesis, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chromodomain-helicase-DNA-binding protein 2 (EC:3.6.4.12)
Short name:
CHD-2
Alternative name(s):
ATP-dependent helicase CHD2
Gene namesi
Name:Chd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:2448567. Chd2.

Subcellular locationi

Nucleus
Note: Binds to myogenic gene promoters.1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Growth delay late in embryogenesis and perinatal lethality (1 Publication). Heterozygous mice show decreased neonatal viability. Heterozygous mice display glomerulopathy, proteinuria and impaired kidney function. Glomerulopathy may be associated with anemia (1 Publication). Heterozygous mutant also show increased extramedullary hematopoiesis and susceptibility to lymphomas, with defects in hematopoietic stem cell differentiation (1 Publication).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18271827Chromodomain-helicase-DNA-binding protein 2
PRO_0000425206Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei207 – 2071Phosphoserine By similarity
Modified residuei208 – 2081Phosphoserine By similarity
Modified residuei240 – 2401Phosphothreonine By similarity
Modified residuei242 – 2421Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiE9PZM4.
PRIDEiE9PZM4.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

ArrayExpressiE9PZM4.
BgeeiE9PZM4.

Interactioni

Subunit structurei

Interacts with MYOD1. Interacts with histone H3.3.

Structurei

3D structure databases

ProteinModelPortaliE9PZM4.
SMRiE9PZM4. Positions 261-343, 1129-1328.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini261 – 35393Chromo 1
Add
BLAST
Domaini378 – 45679Chromo 2
Add
BLAST
Domaini496 – 666171Helicase ATP-binding
Add
BLAST
Domaini795 – 946152Helicase C-terminal
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi617 – 6204DEAH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi13 – 7563Ser-rich
Add
BLAST
Compositional biasi209 – 2157Poly-Asp
Compositional biasi966 – 1066101Glu-rich
Add
BLAST
Compositional biasi1303 – 1439137Lys-rich
Add
BLAST
Compositional biasi1562 – 15654Poly-Glu
Compositional biasi1596 – 1720125His-rich
Add
BLAST

Sequence similaritiesi

Contains 2 chromo domains.

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00560000076896.
KOiK11367.
OMAiKRPYEQY.
TreeFamiTF313461.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
IPR025260. DUF4208.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00385. Chromo. 2 hits.
PF13907. DUF4208. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 2 hits.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF54160. SSF54160. 2 hits.
PROSITEiPS00598. CHROMO_1. 2 hits.
PS50013. CHROMO_2. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E9PZM4-1 [UniParc]FASTAAdd to Basket

« Hide

MMRNKDKSQE EDSSLHSNAS SRSASEEVSG SDSGSQSESE QGSEPGSGHG     50
SESNSSSESS ESQSESESES AGSKSQPVLP EAKEKPASKK ERIADVKKMW 100
EEYPDVYGVR RSNRSRQEPS RFNVKEEASS GSESGSPKRR GQRQLKKQEK 150
WKQDPSEDEQ EQGTSAESEA EQKKGKARRP VPRRTVPKPQ VKKQPKIQRG 200
KRKKQESSDD DDDDDEAPKR QTRRRAAKNV SYKEDDDFET DSDDLIEMTG 250
EGGDEQQDNS ETIEKVLDSR LGKKGATGAS TTVYAVEANG DPSDDFDTER 300
EEGEVQYLIK WKGWSYIHST WESEDSLQQQ KVKGLKKLEN FKKKEDEVKQ 350
WLGKVSPEDV EYFSCQQELA SELNKQYQIV ERVIAVKTSK STLGQTDFPA 400
HSRKPAPSNE PEYLCKWMGL PYSECSWEDE ALIGKKFQNC IDSFHSRNNS 450
KTIPTRECKA LKQRPRFVAL KKQPAYLGGE SLELRDYQLE GLNWLAHSWC 500
KSNSVILADE MGLGKTIQTI SFLSYLFHQH QLYGPFLIVV PLSTLTSWQR 550
EFEIWAPEIN VVVYIGDLMS RNTIREYEWI HSQTKRLKFN ALITTYEILL 600
KDKTVLGSIN WAFLGVDEAH RLKNDDSLLY KTLIDFKSNH RLLITGTPLQ 650
NSLKELWSLL HFIMPEKFEF WEDFEEDHGK GRENGYQSLH KVLEPFLLRR 700
VKKDVEKSLP AKVEQILRVE MSALQKQYYK WILTRNYKAL AKGTRGSTSG 750
FLNIVMELKK CCNHCYLIKA PEDSERESGQ EVLQSLIRSS GKLILLDKLL 800
TRLRERGNRV LIFSQMVRML DILAEYLTIK HYPFQRLDGS IKGEIRKQAL 850
DHFNADGSED FCFLLSTRAG GLGINLASAD TVVIFDSDWN PQNDLQAQAR 900
AHRIGQKKQV NIYRLVTKGT VEEEIIERAK KKMVLDHLVI QRMDTTGRTV 950
LENNSGRSNS NPFNKEELTA ILKFGAEDLF KEIEGEESEP QEMDIDEILR 1000
LAETRENEVS TSATDELLSQ FKVANFATME DEEELEERPH KDWDEIIPEE 1050
QRKKVEEEER QKELEEIYML PRIRSSTKKA QTNDSDSDTE SKRQAQRSSA 1100
SESETDDSDD DKKPKRRGRP RSVRKDLVEG FTDAEIRRFI KAYKKFGLPL 1150
ERLECIARDA ELVDKSVADL KRLGELIHNS CVSAMQEYEE QLKESTSEGK 1200
GPGKRRGPTI KISGVQVNVK SIIQHEEEFE MLHKSIPVDP EEKKKYCLTC 1250
RVKAAHFDVE WGVEDDSRLL LGIYEHGYGN WELIKTDPEL KLTDKILPVE 1300
TDKKPQGKQL QTRVDYLLKL LRKGLEKKGT VASGEEAKLK KRKPRVKKEN 1350
KAPRLKDEHG LEPASPRHSD NPSEEGEVKD DGLEKSPTKK KQKKKENKEN 1400
KEKPVSSRKD REGDKERKKS KDKKEKVKGG DGKSSSKSKR SQGPVHITAG 1450
SEPVPIGEDE DDDLDQETFS ICKERMRPVK KALKQLDKPD KGLSVQEQLE 1500
HTRNCLLKIG DRIAECLKAY SDQEHIKLWR RNLWIFVSKF TEFDARKLHK 1550
LYKMAHKKRS QEEEEQKKKD DSLGGKKPFR PEASGSSRDS LISQSHTSHN 1600
LHPQKPHLPA SHGPQMHGHP RDNYSHPNKR HFSNADRGDW QRERKFNYGG 1650
GNSAPWGGDR HHQYEQHWYK DHHYGDRRHM DAHRSGSYRP NNMSRKRPYE 1700
QYNSDRDHRG HRDYYDRHHH DSKRRRSDDF RPQNYHQQDF RRMSDHRPTM 1750
GYHGQGPSDH YRSFHTDKLG EYKQPMPSLH TALSDPRSPP SQKSPHDSKS 1800
PLDHRSPLER SLEQKNNPDY NWNVRKT 1827
Length:1,827
Mass (Da):210,804
Last modified:April 5, 2011 - v1
Checksum:i60B317C82F373453
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC099699 Genomic DNA. No translation available.
AC154883 Genomic DNA. No translation available.
CCDSiCCDS52274.1.
RefSeqiNP_001074814.2. NM_001081345.2.
UniGeneiMm.34539.
Mm.34955.

Genome annotation databases

EnsembliENSMUST00000169922; ENSMUSP00000126352; ENSMUSG00000078671.
GeneIDi244059.
KEGGimmu:244059.
UCSCiuc009hrd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC099699 Genomic DNA. No translation available.
AC154883 Genomic DNA. No translation available.
CCDSi CCDS52274.1.
RefSeqi NP_001074814.2. NM_001081345.2.
UniGenei Mm.34539.
Mm.34955.

3D structure databases

ProteinModelPortali E9PZM4.
SMRi E9PZM4. Positions 261-343, 1129-1328.
ModBasei Search...
MobiDBi Search...

Proteomic databases

MaxQBi E9PZM4.
PRIDEi E9PZM4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000169922 ; ENSMUSP00000126352 ; ENSMUSG00000078671 .
GeneIDi 244059.
KEGGi mmu:244059.
UCSCi uc009hrd.2. mouse.

Organism-specific databases

CTDi 1106.
MGIi MGI:2448567. Chd2.

Phylogenomic databases

GeneTreei ENSGT00560000076896.
KOi K11367.
OMAi KRPYEQY.
TreeFami TF313461.

Miscellaneous databases

ChiTaRSi CHD2. mouse.
NextBioi 386098.
PROi E9PZM4.
SOURCEi Search...

Gene expression databases

ArrayExpressi E9PZM4.
Bgeei E9PZM4.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
IPR025260. DUF4208.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view ]
Pfami PF00385. Chromo. 2 hits.
PF13907. DUF4208. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view ]
SMARTi SM00298. CHROMO. 2 hits.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF54160. SSF54160. 2 hits.
PROSITEi PS00598. CHROMO_1. 2 hits.
PS50013. CHROMO_2. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "Mutation of the SNF2 family member Chd2 affects mouse development and survival."
    Marfella C.G., Ohkawa Y., Coles A.H., Garlick D.S., Jones S.N., Imbalzano A.N.
    J. Cell. Physiol. 209:162-171(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  3. Erratum
    Marfella C.G., Ohkawa Y., Coles A.H., Garlick D.S., Jones S.N., Imbalzano A.N.
    J. Cell. Physiol. 212:562-562(2007)
  4. "A mutation in the mouse Chd2 chromatin remodeling enzyme results in a complex renal phenotype."
    Marfella C.G., Henninger N., LeBlanc S.E., Krishnan N., Garlick D.S., Holzman L.B., Imbalzano A.N.
    Kidney Blood Press. Res. 31:421-432(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  5. "Role of chromodomain helicase DNA-binding protein 2 in DNA damage response signaling and tumorigenesis."
    Nagarajan P., Onami T.M., Rajagopalan S., Kania S., Donnell R., Venkatachalam S.
    Oncogene 28:1053-1062(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. Cited for: FUNCTION, SUBCELLULAR LOCATION, DNA-BINDING.

Entry informationi

Entry nameiCHD2_MOUSE
AccessioniPrimary (citable) accession number: E9PZM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: April 5, 2011
Last modified: September 3, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi