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E9PZM4

- CHD2_MOUSE

UniProt

E9PZM4 - CHD2_MOUSE

Protein

Chromodomain-helicase-DNA-binding protein 2

Gene

Chd2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 28 (01 Oct 2014)
      Sequence version 1 (05 Apr 2011)
      Previous versions | rss
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    Functioni

    DNA-binding helicase that specifically binds to the promoter of target genes, leading to chromatin remodeling, possibly by promoting deposition of histone H3.3. Involved in myogenesis via interaction with MYOD1: binds to myogenic gene regulatory sequences and mediates incorporation of histone H3.3 prior to the onset of myogenic gene expression, promoting their expression.1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi509 – 5168ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. core promoter sequence-specific DNA binding Source: UniProtKB
    3. helicase activity Source: UniProtKB-KW
    4. histone binding Source: UniProtKB
    5. protein binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: MGI
    2. chromatin modification Source: UniProtKB-KW
    3. hematopoietic stem cell differentiation Source: MGI
    4. muscle organ development Source: UniProtKB
    5. regulation of transcription, DNA-templated Source: UniProtKB-KW
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Helicase, Hydrolase

    Keywords - Biological processi

    Myogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chromodomain-helicase-DNA-binding protein 2 (EC:3.6.4.12)
    Short name:
    CHD-2
    Alternative name(s):
    ATP-dependent helicase CHD2
    Gene namesi
    Name:Chd2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:2448567. Chd2.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Binds to myogenic gene promoters.

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Growth delay late in embryogenesis and perinatal lethality (PubMed:16810678). Heterozygous mice show decreased neonatal viability. Heterozygous mice display glomerulopathy, proteinuria and impaired kidney function. Glomerulopathy may be associated with anemia (PubMed:19142019). Heterozygous mutant also show increased extramedullary hematopoiesis and susceptibility to lymphomas, with defects in hematopoietic stem cell differentiation (PubMed:19137022).3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 18271827Chromodomain-helicase-DNA-binding protein 2PRO_0000425206Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei207 – 2071PhosphoserineBy similarity
    Modified residuei208 – 2081PhosphoserineBy similarity
    Modified residuei240 – 2401PhosphothreonineBy similarity
    Modified residuei242 – 2421PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiE9PZM4.
    PRIDEiE9PZM4.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    ArrayExpressiE9PZM4.
    BgeeiE9PZM4.

    Interactioni

    Subunit structurei

    Interacts with MYOD1. Interacts with histone H3.3.

    Structurei

    3D structure databases

    ProteinModelPortaliE9PZM4.
    SMRiE9PZM4. Positions 261-343, 1129-1328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini261 – 35393Chromo 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini378 – 45679Chromo 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini496 – 666171Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini795 – 946152Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi617 – 6204DEAH box

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi13 – 7563Ser-richAdd
    BLAST
    Compositional biasi209 – 2157Poly-Asp
    Compositional biasi966 – 1066101Glu-richAdd
    BLAST
    Compositional biasi1303 – 1439137Lys-richAdd
    BLAST
    Compositional biasi1562 – 15654Poly-Glu
    Compositional biasi1596 – 1720125His-richAdd
    BLAST

    Sequence similaritiesi

    Contains 2 chromo domains.PROSITE-ProRule annotation
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    GeneTreeiENSGT00560000076896.
    KOiK11367.
    OMAiKRPYEQY.
    TreeFamiTF313461.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR023780. Chromo_domain.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR023779. Chromodomain_CS.
    IPR025260. DUF4208.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000330. SNF2_N.
    [Graphical view]
    PfamiPF00385. Chromo. 2 hits.
    PF13907. DUF4208. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00176. SNF2_N. 1 hit.
    [Graphical view]
    SMARTiSM00298. CHROMO. 2 hits.
    SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF54160. SSF54160. 2 hits.
    PROSITEiPS00598. CHROMO_1. 2 hits.
    PS50013. CHROMO_2. 2 hits.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E9PZM4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMRNKDKSQE EDSSLHSNAS SRSASEEVSG SDSGSQSESE QGSEPGSGHG     50
    SESNSSSESS ESQSESESES AGSKSQPVLP EAKEKPASKK ERIADVKKMW 100
    EEYPDVYGVR RSNRSRQEPS RFNVKEEASS GSESGSPKRR GQRQLKKQEK 150
    WKQDPSEDEQ EQGTSAESEA EQKKGKARRP VPRRTVPKPQ VKKQPKIQRG 200
    KRKKQESSDD DDDDDEAPKR QTRRRAAKNV SYKEDDDFET DSDDLIEMTG 250
    EGGDEQQDNS ETIEKVLDSR LGKKGATGAS TTVYAVEANG DPSDDFDTER 300
    EEGEVQYLIK WKGWSYIHST WESEDSLQQQ KVKGLKKLEN FKKKEDEVKQ 350
    WLGKVSPEDV EYFSCQQELA SELNKQYQIV ERVIAVKTSK STLGQTDFPA 400
    HSRKPAPSNE PEYLCKWMGL PYSECSWEDE ALIGKKFQNC IDSFHSRNNS 450
    KTIPTRECKA LKQRPRFVAL KKQPAYLGGE SLELRDYQLE GLNWLAHSWC 500
    KSNSVILADE MGLGKTIQTI SFLSYLFHQH QLYGPFLIVV PLSTLTSWQR 550
    EFEIWAPEIN VVVYIGDLMS RNTIREYEWI HSQTKRLKFN ALITTYEILL 600
    KDKTVLGSIN WAFLGVDEAH RLKNDDSLLY KTLIDFKSNH RLLITGTPLQ 650
    NSLKELWSLL HFIMPEKFEF WEDFEEDHGK GRENGYQSLH KVLEPFLLRR 700
    VKKDVEKSLP AKVEQILRVE MSALQKQYYK WILTRNYKAL AKGTRGSTSG 750
    FLNIVMELKK CCNHCYLIKA PEDSERESGQ EVLQSLIRSS GKLILLDKLL 800
    TRLRERGNRV LIFSQMVRML DILAEYLTIK HYPFQRLDGS IKGEIRKQAL 850
    DHFNADGSED FCFLLSTRAG GLGINLASAD TVVIFDSDWN PQNDLQAQAR 900
    AHRIGQKKQV NIYRLVTKGT VEEEIIERAK KKMVLDHLVI QRMDTTGRTV 950
    LENNSGRSNS NPFNKEELTA ILKFGAEDLF KEIEGEESEP QEMDIDEILR 1000
    LAETRENEVS TSATDELLSQ FKVANFATME DEEELEERPH KDWDEIIPEE 1050
    QRKKVEEEER QKELEEIYML PRIRSSTKKA QTNDSDSDTE SKRQAQRSSA 1100
    SESETDDSDD DKKPKRRGRP RSVRKDLVEG FTDAEIRRFI KAYKKFGLPL 1150
    ERLECIARDA ELVDKSVADL KRLGELIHNS CVSAMQEYEE QLKESTSEGK 1200
    GPGKRRGPTI KISGVQVNVK SIIQHEEEFE MLHKSIPVDP EEKKKYCLTC 1250
    RVKAAHFDVE WGVEDDSRLL LGIYEHGYGN WELIKTDPEL KLTDKILPVE 1300
    TDKKPQGKQL QTRVDYLLKL LRKGLEKKGT VASGEEAKLK KRKPRVKKEN 1350
    KAPRLKDEHG LEPASPRHSD NPSEEGEVKD DGLEKSPTKK KQKKKENKEN 1400
    KEKPVSSRKD REGDKERKKS KDKKEKVKGG DGKSSSKSKR SQGPVHITAG 1450
    SEPVPIGEDE DDDLDQETFS ICKERMRPVK KALKQLDKPD KGLSVQEQLE 1500
    HTRNCLLKIG DRIAECLKAY SDQEHIKLWR RNLWIFVSKF TEFDARKLHK 1550
    LYKMAHKKRS QEEEEQKKKD DSLGGKKPFR PEASGSSRDS LISQSHTSHN 1600
    LHPQKPHLPA SHGPQMHGHP RDNYSHPNKR HFSNADRGDW QRERKFNYGG 1650
    GNSAPWGGDR HHQYEQHWYK DHHYGDRRHM DAHRSGSYRP NNMSRKRPYE 1700
    QYNSDRDHRG HRDYYDRHHH DSKRRRSDDF RPQNYHQQDF RRMSDHRPTM 1750
    GYHGQGPSDH YRSFHTDKLG EYKQPMPSLH TALSDPRSPP SQKSPHDSKS 1800
    PLDHRSPLER SLEQKNNPDY NWNVRKT 1827
    Length:1,827
    Mass (Da):210,804
    Last modified:April 5, 2011 - v1
    Checksum:i60B317C82F373453
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC099699 Genomic DNA. No translation available.
    AC154883 Genomic DNA. No translation available.
    CCDSiCCDS52274.1.
    RefSeqiNP_001074814.2. NM_001081345.2.
    UniGeneiMm.34539.
    Mm.34955.

    Genome annotation databases

    EnsembliENSMUST00000169922; ENSMUSP00000126352; ENSMUSG00000078671.
    GeneIDi244059.
    KEGGimmu:244059.
    UCSCiuc009hrd.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC099699 Genomic DNA. No translation available.
    AC154883 Genomic DNA. No translation available.
    CCDSi CCDS52274.1.
    RefSeqi NP_001074814.2. NM_001081345.2.
    UniGenei Mm.34539.
    Mm.34955.

    3D structure databases

    ProteinModelPortali E9PZM4.
    SMRi E9PZM4. Positions 261-343, 1129-1328.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    MaxQBi E9PZM4.
    PRIDEi E9PZM4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000169922 ; ENSMUSP00000126352 ; ENSMUSG00000078671 .
    GeneIDi 244059.
    KEGGi mmu:244059.
    UCSCi uc009hrd.2. mouse.

    Organism-specific databases

    CTDi 1106.
    MGIi MGI:2448567. Chd2.

    Phylogenomic databases

    GeneTreei ENSGT00560000076896.
    KOi K11367.
    OMAi KRPYEQY.
    TreeFami TF313461.

    Miscellaneous databases

    ChiTaRSi CHD2. mouse.
    NextBioi 386098.
    PROi E9PZM4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi E9PZM4.
    Bgeei E9PZM4.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR023780. Chromo_domain.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR023779. Chromodomain_CS.
    IPR025260. DUF4208.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000330. SNF2_N.
    [Graphical view ]
    Pfami PF00385. Chromo. 2 hits.
    PF13907. DUF4208. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00176. SNF2_N. 1 hit.
    [Graphical view ]
    SMARTi SM00298. CHROMO. 2 hits.
    SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF54160. SSF54160. 2 hits.
    PROSITEi PS00598. CHROMO_1. 2 hits.
    PS50013. CHROMO_2. 2 hits.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "Mutation of the SNF2 family member Chd2 affects mouse development and survival."
      Marfella C.G., Ohkawa Y., Coles A.H., Garlick D.S., Jones S.N., Imbalzano A.N.
      J. Cell. Physiol. 209:162-171(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    3. Erratum
      Marfella C.G., Ohkawa Y., Coles A.H., Garlick D.S., Jones S.N., Imbalzano A.N.
      J. Cell. Physiol. 212:562-562(2007)
    4. "A mutation in the mouse Chd2 chromatin remodeling enzyme results in a complex renal phenotype."
      Marfella C.G., Henninger N., LeBlanc S.E., Krishnan N., Garlick D.S., Holzman L.B., Imbalzano A.N.
      Kidney Blood Press. Res. 31:421-432(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    5. "Role of chromodomain helicase DNA-binding protein 2 in DNA damage response signaling and tumorigenesis."
      Nagarajan P., Onami T.M., Rajagopalan S., Kania S., Donnell R., Venkatachalam S.
      Oncogene 28:1053-1062(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    6. Cited for: FUNCTION, SUBCELLULAR LOCATION, DNA-BINDING.

    Entry informationi

    Entry nameiCHD2_MOUSE
    AccessioniPrimary (citable) accession number: E9PZM4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 22, 2014
    Last sequence update: April 5, 2011
    Last modified: October 1, 2014
    This is version 28 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3