Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

E9PZM4 (CHD2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chromodomain-helicase-DNA-binding protein 2

Short name=CHD-2
EC=3.6.4.12
Alternative name(s):
ATP-dependent helicase CHD2
Gene names
Name:Chd2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1827 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-binding helicase that specifically binds to the promoter of target genes, leading to chromatin remodeling, possibly by promoting deposition of histone H3.3. Involved in myogenesis via interaction with MYOD1: binds to myogenic gene regulatory sequences and mediates incorporation of histone H3.3 prior to the onset of myogenic gene expression, promoting their expression. Ref.6

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with MYOD1. Interacts with histone H3.3.

Subcellular location

Nucleus. Note: Binds to myogenic gene promoters. Ref.6

Tissue specificity

Widely expressed. Ref.2

Disruption phenotype

Growth delay late in embryogenesis and perinatal lethality (Ref.2). Heterozygous mice show decreased neonatal viability. Heterozygous mice display glomerulopathy, proteinuria and impaired kidney function. Glomerulopathy may be associated with anemia (Ref.4). Heterozygous mutant also show increased extramedullary hematopoiesis and susceptibility to lymphomas, with defects in hematopoietic stem cell differentiation (Ref.5). Ref.2 Ref.4 Ref.5

Sequence similarities

Contains 2 chromo domains.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18271827Chromodomain-helicase-DNA-binding protein 2
PRO_0000425206

Regions

Domain261 – 35393Chromo 1
Domain378 – 45679Chromo 2
Domain496 – 666171Helicase ATP-binding
Domain795 – 946152Helicase C-terminal
Nucleotide binding509 – 5168ATP By similarity
Motif617 – 6204DEAH box
Compositional bias13 – 7563Ser-rich
Compositional bias209 – 2157Poly-Asp
Compositional bias966 – 1066101Glu-rich
Compositional bias1303 – 1439137Lys-rich
Compositional bias1562 – 15654Poly-Glu
Compositional bias1596 – 1720125His-rich

Amino acid modifications

Modified residue2071Phosphoserine By similarity
Modified residue2081Phosphoserine By similarity
Modified residue2401Phosphothreonine By similarity
Modified residue2421Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
E9PZM4 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: 60B317C82F373453

FASTA1,827210,804
        10         20         30         40         50         60 
MMRNKDKSQE EDSSLHSNAS SRSASEEVSG SDSGSQSESE QGSEPGSGHG SESNSSSESS 

        70         80         90        100        110        120 
ESQSESESES AGSKSQPVLP EAKEKPASKK ERIADVKKMW EEYPDVYGVR RSNRSRQEPS 

       130        140        150        160        170        180 
RFNVKEEASS GSESGSPKRR GQRQLKKQEK WKQDPSEDEQ EQGTSAESEA EQKKGKARRP 

       190        200        210        220        230        240 
VPRRTVPKPQ VKKQPKIQRG KRKKQESSDD DDDDDEAPKR QTRRRAAKNV SYKEDDDFET 

       250        260        270        280        290        300 
DSDDLIEMTG EGGDEQQDNS ETIEKVLDSR LGKKGATGAS TTVYAVEANG DPSDDFDTER 

       310        320        330        340        350        360 
EEGEVQYLIK WKGWSYIHST WESEDSLQQQ KVKGLKKLEN FKKKEDEVKQ WLGKVSPEDV 

       370        380        390        400        410        420 
EYFSCQQELA SELNKQYQIV ERVIAVKTSK STLGQTDFPA HSRKPAPSNE PEYLCKWMGL 

       430        440        450        460        470        480 
PYSECSWEDE ALIGKKFQNC IDSFHSRNNS KTIPTRECKA LKQRPRFVAL KKQPAYLGGE 

       490        500        510        520        530        540 
SLELRDYQLE GLNWLAHSWC KSNSVILADE MGLGKTIQTI SFLSYLFHQH QLYGPFLIVV 

       550        560        570        580        590        600 
PLSTLTSWQR EFEIWAPEIN VVVYIGDLMS RNTIREYEWI HSQTKRLKFN ALITTYEILL 

       610        620        630        640        650        660 
KDKTVLGSIN WAFLGVDEAH RLKNDDSLLY KTLIDFKSNH RLLITGTPLQ NSLKELWSLL 

       670        680        690        700        710        720 
HFIMPEKFEF WEDFEEDHGK GRENGYQSLH KVLEPFLLRR VKKDVEKSLP AKVEQILRVE 

       730        740        750        760        770        780 
MSALQKQYYK WILTRNYKAL AKGTRGSTSG FLNIVMELKK CCNHCYLIKA PEDSERESGQ 

       790        800        810        820        830        840 
EVLQSLIRSS GKLILLDKLL TRLRERGNRV LIFSQMVRML DILAEYLTIK HYPFQRLDGS 

       850        860        870        880        890        900 
IKGEIRKQAL DHFNADGSED FCFLLSTRAG GLGINLASAD TVVIFDSDWN PQNDLQAQAR 

       910        920        930        940        950        960 
AHRIGQKKQV NIYRLVTKGT VEEEIIERAK KKMVLDHLVI QRMDTTGRTV LENNSGRSNS 

       970        980        990       1000       1010       1020 
NPFNKEELTA ILKFGAEDLF KEIEGEESEP QEMDIDEILR LAETRENEVS TSATDELLSQ 

      1030       1040       1050       1060       1070       1080 
FKVANFATME DEEELEERPH KDWDEIIPEE QRKKVEEEER QKELEEIYML PRIRSSTKKA 

      1090       1100       1110       1120       1130       1140 
QTNDSDSDTE SKRQAQRSSA SESETDDSDD DKKPKRRGRP RSVRKDLVEG FTDAEIRRFI 

      1150       1160       1170       1180       1190       1200 
KAYKKFGLPL ERLECIARDA ELVDKSVADL KRLGELIHNS CVSAMQEYEE QLKESTSEGK 

      1210       1220       1230       1240       1250       1260 
GPGKRRGPTI KISGVQVNVK SIIQHEEEFE MLHKSIPVDP EEKKKYCLTC RVKAAHFDVE 

      1270       1280       1290       1300       1310       1320 
WGVEDDSRLL LGIYEHGYGN WELIKTDPEL KLTDKILPVE TDKKPQGKQL QTRVDYLLKL 

      1330       1340       1350       1360       1370       1380 
LRKGLEKKGT VASGEEAKLK KRKPRVKKEN KAPRLKDEHG LEPASPRHSD NPSEEGEVKD 

      1390       1400       1410       1420       1430       1440 
DGLEKSPTKK KQKKKENKEN KEKPVSSRKD REGDKERKKS KDKKEKVKGG DGKSSSKSKR 

      1450       1460       1470       1480       1490       1500 
SQGPVHITAG SEPVPIGEDE DDDLDQETFS ICKERMRPVK KALKQLDKPD KGLSVQEQLE 

      1510       1520       1530       1540       1550       1560 
HTRNCLLKIG DRIAECLKAY SDQEHIKLWR RNLWIFVSKF TEFDARKLHK LYKMAHKKRS 

      1570       1580       1590       1600       1610       1620 
QEEEEQKKKD DSLGGKKPFR PEASGSSRDS LISQSHTSHN LHPQKPHLPA SHGPQMHGHP 

      1630       1640       1650       1660       1670       1680 
RDNYSHPNKR HFSNADRGDW QRERKFNYGG GNSAPWGGDR HHQYEQHWYK DHHYGDRRHM 

      1690       1700       1710       1720       1730       1740 
DAHRSGSYRP NNMSRKRPYE QYNSDRDHRG HRDYYDRHHH DSKRRRSDDF RPQNYHQQDF 

      1750       1760       1770       1780       1790       1800 
RRMSDHRPTM GYHGQGPSDH YRSFHTDKLG EYKQPMPSLH TALSDPRSPP SQKSPHDSKS 

      1810       1820 
PLDHRSPLER SLEQKNNPDY NWNVRKT 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"Mutation of the SNF2 family member Chd2 affects mouse development and survival."
Marfella C.G., Ohkawa Y., Coles A.H., Garlick D.S., Jones S.N., Imbalzano A.N.
J. Cell. Physiol. 209:162-171(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
[3]Erratum
Marfella C.G., Ohkawa Y., Coles A.H., Garlick D.S., Jones S.N., Imbalzano A.N.
J. Cell. Physiol. 212:562-562(2007)
[4]"A mutation in the mouse Chd2 chromatin remodeling enzyme results in a complex renal phenotype."
Marfella C.G., Henninger N., LeBlanc S.E., Krishnan N., Garlick D.S., Holzman L.B., Imbalzano A.N.
Kidney Blood Press. Res. 31:421-432(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[5]"Role of chromodomain helicase DNA-binding protein 2 in DNA damage response signaling and tumorigenesis."
Nagarajan P., Onami T.M., Rajagopalan S., Kania S., Donnell R., Venkatachalam S.
Oncogene 28:1053-1062(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[6]"Chd2 interacts with H3.3 to determine myogenic cell fate."
Harada A., Okada S., Konno D., Odawara J., Yoshimi T., Yoshimura S., Kumamaru H., Saiwai H., Tsubota T., Kurumizaka H., Akashi K., Tachibana T., Imbalzano A.N., Ohkawa Y.
EMBO J. 31:2994-3007(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DNA-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC099699 Genomic DNA. No translation available.
AC154883 Genomic DNA. No translation available.
RefSeqNP_001074814.2. NM_001081345.2.
UniGeneMm.34539.
Mm.34955.

3D structure databases

ProteinModelPortalE9PZM4.
SMRE9PZM4. Positions 261-343, 1129-1328.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

MaxQBE9PZM4.
PRIDEE9PZM4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000169922; ENSMUSP00000126352; ENSMUSG00000078671.
GeneID244059.
KEGGmmu:244059.
UCSCuc009hrd.2. mouse.

Organism-specific databases

CTD1106.
MGIMGI:2448567. Chd2.

Phylogenomic databases

GeneTreeENSGT00560000076896.
KOK11367.
OMAKRPYEQY.
TreeFamTF313461.

Gene expression databases

ArrayExpressE9PZM4.
BgeeE9PZM4.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
IPR025260. DUF4208.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamPF00385. Chromo. 2 hits.
PF13907. DUF4208. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTSM00298. CHROMO. 2 hits.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF54160. SSF54160. 2 hits.
PROSITEPS00598. CHROMO_1. 2 hits.
PS50013. CHROMO_2. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCHD2. mouse.
NextBio386098.
PROE9PZM4.
SOURCESearch...

Entry information

Entry nameCHD2_MOUSE
AccessionPrimary (citable) accession number: E9PZM4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: April 5, 2011
Last modified: June 11, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot