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E9PZJ8

- ASCC3_MOUSE

UniProt

E9PZJ8 - ASCC3_MOUSE

Protein

Activating signal cointegrator 1 complex subunit 3

Gene

Ascc3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 31 (01 Oct 2014)
      Sequence version 1 (05 Apr 2011)
      Previous versions | rss
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    Functioni

    3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3, enabling ALKHB3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions. Enhances NF-kappa-B, SRF and AP1 transactivation By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi500 – 5078ATPPROSITE-ProRule annotation
    Nucleotide bindingi1350 – 13578ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent 3'-5' DNA helicase activity Source: UniProtKB
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. cell proliferation Source: UniProtKB
    2. DNA dealkylation involved in DNA repair Source: UniProtKB
    3. DNA duplex unwinding Source: UniProtKB
    4. regulation of transcription, DNA-templated Source: UniProtKB-KW
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Activating signal cointegrator 1 complex subunit 3 (EC:3.6.4.12)
    Gene namesi
    Name:Ascc3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1925237. Ascc3.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity

    GO - Cellular componenti

    1. Golgi apparatus Source: Ensembl
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 21982198Activating signal cointegrator 1 complex subunit 3PRO_0000416915Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei573 – 5731N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiE9PZJ8.
    PRIDEiE9PZJ8.

    Expressioni

    Gene expression databases

    BgeeiE9PZJ8.

    Interactioni

    Subunit structurei

    Part of TRIP4 complex, that contains ASCC1, ASCC2 and ASCC3. The TRIP4 complex interacts with ALKBH3 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000036726.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini487 – 670184Helicase ATP-binding 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini697 – 915219Helicase C-terminal 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini979 – 1288310SEC63 1Add
    BLAST
    Domaini1337 – 1512176Helicase ATP-binding 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1545 – 1740196Helicase C-terminal 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1813 – 2177365SEC63 2Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili18 – 8164Sequence AnalysisAdd
    BLAST
    Coiled coili328 – 35629Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi612 – 6154DEVH box
    Motifi1454 – 14574DEIH box

    Sequence similaritiesi

    Belongs to the helicase family.Curated
    Contains 2 helicase ATP-binding domains.PROSITE-ProRule annotation
    Contains 2 helicase C-terminal domains.PROSITE-ProRule annotation
    Contains 2 SEC63 domains.Curated

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    GeneTreeiENSGT00640000091272.
    HOGENOMiHOG000157749.
    KOiK01529.
    OMAiVHMLHDE.
    OrthoDBiEOG7PGDPQ.
    TreeFamiTF105778.

    Family and domain databases

    Gene3Di2.60.40.150. 1 hit.
    3.40.50.300. 4 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR000008. C2_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR014756. Ig_E-set.
    IPR027417. P-loop_NTPase.
    IPR004179. Sec63-dom.
    [Graphical view]
    PfamiPF00270. DEAD. 2 hits.
    PF00271. Helicase_C. 2 hits.
    PF02889. Sec63. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM00487. DEXDc. 2 hits.
    SM00490. HELICc. 2 hits.
    SM00611. SEC63. 2 hits.
    SM00973. Sec63. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 4 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 2 hits.
    PS51194. HELICASE_CTER. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: E9PZJ8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MALPRLTGAL RSFSNVTKQD NYNEEVADLK LKRSKLHEQV LDFGLTWKKI     50
    VKFLNEKLEK NKMQNINEDL KDILQAAKQI VGTDNGREAI ESGAAFLFMT 100
    FHMTDSVGYM ETKAIRQTFG PFPSSSATSA CNATNRIISH FSQDDLTAFV 150
    QMAENPCNDR VVFGKNLAFS FDMYDLDHFD ELPINGESQK TISLDYKKFL 200
    NEQFQEPYTP ELKPVEKTNG SLLWCEVEKY LNATLKEMTE AARVEDLCCT 250
    LYDMLASAKS GDELQDELFE LLGPEGLDLI EKLLQNRITI VDRFLNSSSD 300
    HKFQVLQDSC KKILGENSKP NYGCQVTIQS EQEKQLMKQY RREEKRIARR 350
    EKKAGEDGEV SGEGVLPFDP KELRIQREHA LLNARNAPIL GRQRDVEFEK 400
    IRYPHVYDSQ AQARETSAFI AGAKMILPEG IQRENTKLYE EVRIPYGEPM 450
    PVGFEEKPVY IKDLDEVGQL AFKGMKRLNR IQSIVFETAY NTNENMLICA 500
    PTGAGKTNIA MLTILHEIRQ HFHQGVIKKN EFKIVYVAPM KALAAEMTNY 550
    FSKRLEPLGI VVKELTGDMQ LSKSEILRTQ MLVTTPEKWD VVTRKSVGDV 600
    ALSQIVKLLI LDEVHLLHED RGPVLESIVA RTLRQVESTQ SMIRILGLSA 650
    TLPNYLDVAT FLHVNPYIGL FYFDGRFRPV PLGQTFLGIK STNKMQQLNN 700
    MDEVCYESVL KQVKAGHQVM VFVHARNATV RTAMSLIERA KNSGQISCFL 750
    PTEGPEYGHA LKQVQKSRNK QVRELFSDGF SIHHAGMLRQ DRNLVENLFS 800
    NGHIKVLVCT ATLAWGVNLP AHAVVIKGTQ IYAAKRGSFV DLGILDVMQI 850
    FGRAGRPQFD KFGEGIIITT HDKLSHYLSL LTQQNPIESQ FLESLADNLN 900
    AEIALGTVTN VEEAVRWMSY TYLYVRMRAN PLAYGISHKA YQIDPTLRKH 950
    REQLLIEVGQ KLDKAKMIRF EERTGYFSST DLGRTASHFY IKYNTIETFN 1000
    ELFDAHKTEG DIFAIVSKAE EFDQIKVREE EIEELDALLN NFCELSAPGG 1050
    VENSYGKINI LLQTYISRGE MDSFSLISDS AYVAQNAARI VRALFEIALR 1100
    KRWPTMTYRL LNLSKVIDKR LWGWASPLRQ FSVLPPHILT RLEEKNLTVD 1150
    KLKDMRKDEI GHILHHVNIG LKVKQCVHQI PSVTMEASIQ PITRTVLRVS 1200
    LNIHPDFSWN DQVHGTVGEP WWIWVEDPTN DHIYHSEYFL ALKKQVINKE 1250
    AQLLVFTIPI FEPLPSQYYI RAVSDRWLGA EAVCIINFQH LILPERHPPH 1300
    TELLDLQPLP ITALGCKAYE ALYNFSHFNP VQTQIFHTLY HTDCNVLLGA 1350
    PTGSGKTVAA ELAIFRVFNK YPTSKAVYIA PLKALVRERM DDWKIRIEEK 1400
    LGKKVIELTG DVTPDMKSIA KADLIVTTPE KWDGVSRSWQ NRSYVQQVNI 1450
    LIIDEIHLLG EERGPVLEVI VSRTNFISSH TEKPVRIVGL STALANARDL 1500
    ADWLNIKQMG LFNFRPSVRP VPLEVHIQGF PGQHYCPRMA SMNKPAFQAI 1550
    RSHSPAKPVL IFVSSRRQTR LTALELIAFL ATEEDPKQWL NMDEQEMDNI 1600
    IGTVRDSNLK LTLAFGIGMH HAGLHERDRK TVEELFVNCK VQVLIATSTL 1650
    AWGVNFPAHL VIIKGTEYYD GKTRRYVDFP ITDVLQMMGR AGRPQFDDQG 1700
    KAVILVHDIK KDFYKKFLYE PFPVESSLLG VLSDHLNAEI AGGTITSKQD 1750
    AMDYITWTYF FRRLIMNPSY YSLGDVSQDS INKFLSHLIG QSLVELELSH 1800
    CIEVGEDNRT IEPLTCGRIA SYYYLKHKTV KMFKDRLKPE CSTEELLSIL 1850
    SDAEEYTDLP VRHNEDHTNN ELAKCLPIEL NPHSFDSPHT KAHLLLQAHL 1900
    SRAMLPCPDY DTDTKTVLDQ ALRVCQAMLD VAASQGWLVT VLNITHLIQM 1950
    VIQGRWLKDS SLLTIPNIEQ HHLHLFRKWK PPVKSSHAKC RTSIECLPEL 2000
    IHACEGKDHV FSSMVEKELQ PAKTKQAWNF LSRLPVINVG ISVKGSWDDL 2050
    VEGHNELSIS TLTADKRDEN KWIKLHADQE YVLQVSLQRV HFGLPKGKHE 2100
    NHAVTPRFPK LKDEGWFLIL GEVDKRELMA VKRVGFVRTH HDASISFFTP 2150
    ETPGRYIFTL YLMSDCYLGL DQQYDIYLNV IKANISTKDS DVFTDLSV 2198
    Length:2,198
    Mass (Da):250,557
    Last modified:April 5, 2011 - v1
    Checksum:i39FF851ED47394FD
    GO
    Isoform 2 (identifier: E9PZJ8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1070: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,128
    Mass (Da):128,982
    Checksum:iF7057317EB545F77
    GO

    Sequence cautioni

    The sequence AAH32189.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1818 – 18181R → L in AAH59917. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 10701070Missing in isoform 2. 1 PublicationVSP_042999Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC137877 Genomic DNA. No translation available.
    AC153529 Genomic DNA. No translation available.
    AC159114 Genomic DNA. No translation available.
    BC032189 mRNA. Translation: AAH32189.1. Different initiation.
    BC059917 mRNA. Translation: AAH59917.1.
    AK021027 mRNA. Translation: BAC25644.1.
    AK052745 mRNA. Translation: BAC35127.1.
    CCDSiCCDS48555.1. [E9PZJ8-1]
    RefSeqiNP_932124.2. NM_198007.2. [E9PZJ8-1]
    UniGeneiMm.222497.

    Genome annotation databases

    EnsembliENSMUST00000035606; ENSMUSP00000036726; ENSMUSG00000038774. [E9PZJ8-1]
    GeneIDi77987.
    KEGGimmu:77987.
    UCSCiuc007fak.2. mouse. [E9PZJ8-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC137877 Genomic DNA. No translation available.
    AC153529 Genomic DNA. No translation available.
    AC159114 Genomic DNA. No translation available.
    BC032189 mRNA. Translation: AAH32189.1 . Different initiation.
    BC059917 mRNA. Translation: AAH59917.1 .
    AK021027 mRNA. Translation: BAC25644.1 .
    AK052745 mRNA. Translation: BAC35127.1 .
    CCDSi CCDS48555.1. [E9PZJ8-1 ]
    RefSeqi NP_932124.2. NM_198007.2. [E9PZJ8-1 ]
    UniGenei Mm.222497.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000036726.

    Proteomic databases

    MaxQBi E9PZJ8.
    PRIDEi E9PZJ8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035606 ; ENSMUSP00000036726 ; ENSMUSG00000038774 . [E9PZJ8-1 ]
    GeneIDi 77987.
    KEGGi mmu:77987.
    UCSCi uc007fak.2. mouse. [E9PZJ8-1 ]

    Organism-specific databases

    CTDi 10973.
    MGIi MGI:1925237. Ascc3.

    Phylogenomic databases

    GeneTreei ENSGT00640000091272.
    HOGENOMi HOG000157749.
    KOi K01529.
    OMAi VHMLHDE.
    OrthoDBi EOG7PGDPQ.
    TreeFami TF105778.

    Miscellaneous databases

    NextBioi 347945.
    PROi E9PZJ8.
    SOURCEi Search...

    Gene expression databases

    Bgeei E9PZJ8.

    Family and domain databases

    Gene3Di 2.60.40.150. 1 hit.
    3.40.50.300. 4 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR000008. C2_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR014756. Ig_E-set.
    IPR027417. P-loop_NTPase.
    IPR004179. Sec63-dom.
    [Graphical view ]
    Pfami PF00270. DEAD. 2 hits.
    PF00271. Helicase_C. 2 hits.
    PF02889. Sec63. 2 hits.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM00487. DEXDc. 2 hits.
    SM00490. HELICc. 2 hits.
    SM00611. SEC63. 2 hits.
    SM00973. Sec63. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 4 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 2 hits.
    PS51194. HELICASE_CTER. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Mammary tumor.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-331 AND 2125-2198.
      Strain: C57BL/6J.
      Tissue: Kidney and Thymus.
    4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-573, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiASCC3_MOUSE
    AccessioniPrimary (citable) accession number: E9PZJ8
    Secondary accession number(s): Q6PB36
    , Q8C1G1, Q8C707, Q8K292
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2012
    Last sequence update: April 5, 2011
    Last modified: October 1, 2014
    This is version 31 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3