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Protein

Activating signal cointegrator 1 complex subunit 3

Gene

Ascc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions. Enhances NF-kappa-B, SRF and AP1 transactivation (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi500 – 507ATPPROSITE-ProRule annotation8
Nucleotide bindingi1350 – 1357ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Activating signal cointegrator 1 complex subunit 3 (EC:3.6.4.12)
Gene namesi
Name:Ascc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1925237. Ascc3.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004169151 – 2198Activating signal cointegrator 1 complex subunit 3Add BLAST2198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineBy similarity1
Modified residuei573N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiE9PZJ8.
MaxQBiE9PZJ8.
PaxDbiE9PZJ8.
PeptideAtlasiE9PZJ8.
PRIDEiE9PZJ8.

PTM databases

iPTMnetiE9PZJ8.
PhosphoSitePlusiE9PZJ8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038774.
GenevisibleiE9PZJ8. MM.

Interactioni

Subunit structurei

Part of the ASC-1 complex, that contains TRIP4, ASCC1, ASCC2 and ASCC3. The ASC-1 complex interacts with ALKBH3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi219071. 1 interactor.
IntActiE9PZJ8. 1 interactor.
STRINGi10090.ENSMUSP00000036726.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini487 – 670Helicase ATP-binding 1PROSITE-ProRule annotationAdd BLAST184
Domaini697 – 915Helicase C-terminal 1PROSITE-ProRule annotationAdd BLAST219
Domaini979 – 1288SEC63 1Add BLAST310
Domaini1337 – 1512Helicase ATP-binding 2PROSITE-ProRule annotationAdd BLAST176
Domaini1545 – 1740Helicase C-terminal 2PROSITE-ProRule annotationAdd BLAST196
Domaini1813 – 2177SEC63 2Add BLAST365

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili18 – 81Sequence analysisAdd BLAST64
Coiled coili328 – 356Sequence analysisAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi612 – 615DEVH box4
Motifi1454 – 1457DEIH box4

Sequence similaritiesi

Belongs to the helicase family.Curated
Contains 2 helicase ATP-binding domains.PROSITE-ProRule annotation
Contains 2 helicase C-terminal domains.PROSITE-ProRule annotation
Contains 2 SEC63 domains.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0952. Eukaryota.
COG1204. LUCA.
GeneTreeiENSGT00640000091272.
HOGENOMiHOG000157749.
InParanoidiE9PZJ8.
KOiK18663.
OMAiFLRVNPM.
OrthoDBiEOG091G0HE5.
TreeFamiTF105778.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR003593. AAA+_ATPase.
IPR000008. C2_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014756. Ig_E-set.
IPR027417. P-loop_NTPase.
IPR004179. Sec63-dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 2 hits.
PF00271. Helicase_C. 2 hits.
PF02889. Sec63. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM00487. DEXDc. 2 hits.
SM00490. HELICc. 2 hits.
SM00973. Sec63. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
SSF52540. SSF52540. 4 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: E9PZJ8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALPRLTGAL RSFSNVTKQD NYNEEVADLK LKRSKLHEQV LDFGLTWKKI
60 70 80 90 100
VKFLNEKLEK NKMQNINEDL KDILQAAKQI VGTDNGREAI ESGAAFLFMT
110 120 130 140 150
FHMTDSVGYM ETKAIRQTFG PFPSSSATSA CNATNRIISH FSQDDLTAFV
160 170 180 190 200
QMAENPCNDR VVFGKNLAFS FDMYDLDHFD ELPINGESQK TISLDYKKFL
210 220 230 240 250
NEQFQEPYTP ELKPVEKTNG SLLWCEVEKY LNATLKEMTE AARVEDLCCT
260 270 280 290 300
LYDMLASAKS GDELQDELFE LLGPEGLDLI EKLLQNRITI VDRFLNSSSD
310 320 330 340 350
HKFQVLQDSC KKILGENSKP NYGCQVTIQS EQEKQLMKQY RREEKRIARR
360 370 380 390 400
EKKAGEDGEV SGEGVLPFDP KELRIQREHA LLNARNAPIL GRQRDVEFEK
410 420 430 440 450
IRYPHVYDSQ AQARETSAFI AGAKMILPEG IQRENTKLYE EVRIPYGEPM
460 470 480 490 500
PVGFEEKPVY IKDLDEVGQL AFKGMKRLNR IQSIVFETAY NTNENMLICA
510 520 530 540 550
PTGAGKTNIA MLTILHEIRQ HFHQGVIKKN EFKIVYVAPM KALAAEMTNY
560 570 580 590 600
FSKRLEPLGI VVKELTGDMQ LSKSEILRTQ MLVTTPEKWD VVTRKSVGDV
610 620 630 640 650
ALSQIVKLLI LDEVHLLHED RGPVLESIVA RTLRQVESTQ SMIRILGLSA
660 670 680 690 700
TLPNYLDVAT FLHVNPYIGL FYFDGRFRPV PLGQTFLGIK STNKMQQLNN
710 720 730 740 750
MDEVCYESVL KQVKAGHQVM VFVHARNATV RTAMSLIERA KNSGQISCFL
760 770 780 790 800
PTEGPEYGHA LKQVQKSRNK QVRELFSDGF SIHHAGMLRQ DRNLVENLFS
810 820 830 840 850
NGHIKVLVCT ATLAWGVNLP AHAVVIKGTQ IYAAKRGSFV DLGILDVMQI
860 870 880 890 900
FGRAGRPQFD KFGEGIIITT HDKLSHYLSL LTQQNPIESQ FLESLADNLN
910 920 930 940 950
AEIALGTVTN VEEAVRWMSY TYLYVRMRAN PLAYGISHKA YQIDPTLRKH
960 970 980 990 1000
REQLLIEVGQ KLDKAKMIRF EERTGYFSST DLGRTASHFY IKYNTIETFN
1010 1020 1030 1040 1050
ELFDAHKTEG DIFAIVSKAE EFDQIKVREE EIEELDALLN NFCELSAPGG
1060 1070 1080 1090 1100
VENSYGKINI LLQTYISRGE MDSFSLISDS AYVAQNAARI VRALFEIALR
1110 1120 1130 1140 1150
KRWPTMTYRL LNLSKVIDKR LWGWASPLRQ FSVLPPHILT RLEEKNLTVD
1160 1170 1180 1190 1200
KLKDMRKDEI GHILHHVNIG LKVKQCVHQI PSVTMEASIQ PITRTVLRVS
1210 1220 1230 1240 1250
LNIHPDFSWN DQVHGTVGEP WWIWVEDPTN DHIYHSEYFL ALKKQVINKE
1260 1270 1280 1290 1300
AQLLVFTIPI FEPLPSQYYI RAVSDRWLGA EAVCIINFQH LILPERHPPH
1310 1320 1330 1340 1350
TELLDLQPLP ITALGCKAYE ALYNFSHFNP VQTQIFHTLY HTDCNVLLGA
1360 1370 1380 1390 1400
PTGSGKTVAA ELAIFRVFNK YPTSKAVYIA PLKALVRERM DDWKIRIEEK
1410 1420 1430 1440 1450
LGKKVIELTG DVTPDMKSIA KADLIVTTPE KWDGVSRSWQ NRSYVQQVNI
1460 1470 1480 1490 1500
LIIDEIHLLG EERGPVLEVI VSRTNFISSH TEKPVRIVGL STALANARDL
1510 1520 1530 1540 1550
ADWLNIKQMG LFNFRPSVRP VPLEVHIQGF PGQHYCPRMA SMNKPAFQAI
1560 1570 1580 1590 1600
RSHSPAKPVL IFVSSRRQTR LTALELIAFL ATEEDPKQWL NMDEQEMDNI
1610 1620 1630 1640 1650
IGTVRDSNLK LTLAFGIGMH HAGLHERDRK TVEELFVNCK VQVLIATSTL
1660 1670 1680 1690 1700
AWGVNFPAHL VIIKGTEYYD GKTRRYVDFP ITDVLQMMGR AGRPQFDDQG
1710 1720 1730 1740 1750
KAVILVHDIK KDFYKKFLYE PFPVESSLLG VLSDHLNAEI AGGTITSKQD
1760 1770 1780 1790 1800
AMDYITWTYF FRRLIMNPSY YSLGDVSQDS INKFLSHLIG QSLVELELSH
1810 1820 1830 1840 1850
CIEVGEDNRT IEPLTCGRIA SYYYLKHKTV KMFKDRLKPE CSTEELLSIL
1860 1870 1880 1890 1900
SDAEEYTDLP VRHNEDHTNN ELAKCLPIEL NPHSFDSPHT KAHLLLQAHL
1910 1920 1930 1940 1950
SRAMLPCPDY DTDTKTVLDQ ALRVCQAMLD VAASQGWLVT VLNITHLIQM
1960 1970 1980 1990 2000
VIQGRWLKDS SLLTIPNIEQ HHLHLFRKWK PPVKSSHAKC RTSIECLPEL
2010 2020 2030 2040 2050
IHACEGKDHV FSSMVEKELQ PAKTKQAWNF LSRLPVINVG ISVKGSWDDL
2060 2070 2080 2090 2100
VEGHNELSIS TLTADKRDEN KWIKLHADQE YVLQVSLQRV HFGLPKGKHE
2110 2120 2130 2140 2150
NHAVTPRFPK LKDEGWFLIL GEVDKRELMA VKRVGFVRTH HDASISFFTP
2160 2170 2180 2190
ETPGRYIFTL YLMSDCYLGL DQQYDIYLNV IKANISTKDS DVFTDLSV
Length:2,198
Mass (Da):250,557
Last modified:April 5, 2011 - v1
Checksum:i39FF851ED47394FD
GO
Isoform 2 (identifier: E9PZJ8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1070: Missing.

Note: No experimental confirmation available.
Show »
Length:1,128
Mass (Da):128,982
Checksum:iF7057317EB545F77
GO

Sequence cautioni

The sequence AAH32189 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1818R → L in AAH59917 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0429991 – 1070Missing in isoform 2. 1 PublicationAdd BLAST1070

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137877 Genomic DNA. No translation available.
AC153529 Genomic DNA. No translation available.
AC159114 Genomic DNA. No translation available.
BC032189 mRNA. Translation: AAH32189.1. Different initiation.
BC059917 mRNA. Translation: AAH59917.1.
AK021027 mRNA. Translation: BAC25644.1.
AK052745 mRNA. Translation: BAC35127.1.
CCDSiCCDS48555.1. [E9PZJ8-1]
RefSeqiNP_932124.2. NM_198007.2. [E9PZJ8-1]
UniGeneiMm.222497.

Genome annotation databases

EnsembliENSMUST00000035606; ENSMUSP00000036726; ENSMUSG00000038774. [E9PZJ8-1]
GeneIDi77987.
KEGGimmu:77987.
UCSCiuc007fak.2. mouse. [E9PZJ8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137877 Genomic DNA. No translation available.
AC153529 Genomic DNA. No translation available.
AC159114 Genomic DNA. No translation available.
BC032189 mRNA. Translation: AAH32189.1. Different initiation.
BC059917 mRNA. Translation: AAH59917.1.
AK021027 mRNA. Translation: BAC25644.1.
AK052745 mRNA. Translation: BAC35127.1.
CCDSiCCDS48555.1. [E9PZJ8-1]
RefSeqiNP_932124.2. NM_198007.2. [E9PZJ8-1]
UniGeneiMm.222497.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219071. 1 interactor.
IntActiE9PZJ8. 1 interactor.
STRINGi10090.ENSMUSP00000036726.

PTM databases

iPTMnetiE9PZJ8.
PhosphoSitePlusiE9PZJ8.

Proteomic databases

EPDiE9PZJ8.
MaxQBiE9PZJ8.
PaxDbiE9PZJ8.
PeptideAtlasiE9PZJ8.
PRIDEiE9PZJ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035606; ENSMUSP00000036726; ENSMUSG00000038774. [E9PZJ8-1]
GeneIDi77987.
KEGGimmu:77987.
UCSCiuc007fak.2. mouse. [E9PZJ8-1]

Organism-specific databases

CTDi10973.
MGIiMGI:1925237. Ascc3.

Phylogenomic databases

eggNOGiKOG0952. Eukaryota.
COG1204. LUCA.
GeneTreeiENSGT00640000091272.
HOGENOMiHOG000157749.
InParanoidiE9PZJ8.
KOiK18663.
OMAiFLRVNPM.
OrthoDBiEOG091G0HE5.
TreeFamiTF105778.

Miscellaneous databases

PROiE9PZJ8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038774.
GenevisibleiE9PZJ8. MM.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR003593. AAA+_ATPase.
IPR000008. C2_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014756. Ig_E-set.
IPR027417. P-loop_NTPase.
IPR004179. Sec63-dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 2 hits.
PF00271. Helicase_C. 2 hits.
PF02889. Sec63. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM00487. DEXDc. 2 hits.
SM00490. HELICc. 2 hits.
SM00973. Sec63. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
SSF52540. SSF52540. 4 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASCC3_MOUSE
AccessioniPrimary (citable) accession number: E9PZJ8
Secondary accession number(s): Q6PB36
, Q8C1G1, Q8C707, Q8K292
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: April 5, 2011
Last modified: November 30, 2016
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.