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E9PZJ8 (ASCC3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Activating signal cointegrator 1 complex subunit 3

EC=3.6.4.12
Gene names
Name:Ascc3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2198 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3, enabling ALKHB3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions. Enhances NF-kappa-B, SRF and AP1 transactivation By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Part of TRIP4 complex, that contains ASCC1, ASCC2 and ASCC3. The TRIP4 complex interacts with ALKBH3 By similarity.

Subcellular location

Nucleus By similarity. Cytoplasm By similarity.

Sequence similarities

Belongs to the helicase family.

Contains 2 helicase ATP-binding domains.

Contains 2 helicase C-terminal domains.

Contains 2 SEC63 domains.

Sequence caution

The sequence AAH32189.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: E9PZJ8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: E9PZJ8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1070: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 21982198Activating signal cointegrator 1 complex subunit 3
PRO_0000416915

Regions

Domain487 – 670184Helicase ATP-binding 1
Domain697 – 915219Helicase C-terminal 1
Domain979 – 1288310SEC63 1
Domain1337 – 1512176Helicase ATP-binding 2
Domain1545 – 1740196Helicase C-terminal 2
Domain1813 – 2177365SEC63 2
Nucleotide binding500 – 5078ATP By similarity
Nucleotide binding1350 – 13578ATP By similarity
Coiled coil18 – 8164 Potential
Coiled coil328 – 35629 Potential
Motif612 – 6154DEVH box
Motif1454 – 14574DEIH box

Amino acid modifications

Modified residue5731N6-acetyllysine Ref.4

Natural variations

Alternative sequence1 – 10701070Missing in isoform 2.
VSP_042999

Experimental info

Sequence conflict18181R → L in AAH59917. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: 39FF851ED47394FD

FASTA2,198250,557
        10         20         30         40         50         60 
MALPRLTGAL RSFSNVTKQD NYNEEVADLK LKRSKLHEQV LDFGLTWKKI VKFLNEKLEK 

        70         80         90        100        110        120 
NKMQNINEDL KDILQAAKQI VGTDNGREAI ESGAAFLFMT FHMTDSVGYM ETKAIRQTFG 

       130        140        150        160        170        180 
PFPSSSATSA CNATNRIISH FSQDDLTAFV QMAENPCNDR VVFGKNLAFS FDMYDLDHFD 

       190        200        210        220        230        240 
ELPINGESQK TISLDYKKFL NEQFQEPYTP ELKPVEKTNG SLLWCEVEKY LNATLKEMTE 

       250        260        270        280        290        300 
AARVEDLCCT LYDMLASAKS GDELQDELFE LLGPEGLDLI EKLLQNRITI VDRFLNSSSD 

       310        320        330        340        350        360 
HKFQVLQDSC KKILGENSKP NYGCQVTIQS EQEKQLMKQY RREEKRIARR EKKAGEDGEV 

       370        380        390        400        410        420 
SGEGVLPFDP KELRIQREHA LLNARNAPIL GRQRDVEFEK IRYPHVYDSQ AQARETSAFI 

       430        440        450        460        470        480 
AGAKMILPEG IQRENTKLYE EVRIPYGEPM PVGFEEKPVY IKDLDEVGQL AFKGMKRLNR 

       490        500        510        520        530        540 
IQSIVFETAY NTNENMLICA PTGAGKTNIA MLTILHEIRQ HFHQGVIKKN EFKIVYVAPM 

       550        560        570        580        590        600 
KALAAEMTNY FSKRLEPLGI VVKELTGDMQ LSKSEILRTQ MLVTTPEKWD VVTRKSVGDV 

       610        620        630        640        650        660 
ALSQIVKLLI LDEVHLLHED RGPVLESIVA RTLRQVESTQ SMIRILGLSA TLPNYLDVAT 

       670        680        690        700        710        720 
FLHVNPYIGL FYFDGRFRPV PLGQTFLGIK STNKMQQLNN MDEVCYESVL KQVKAGHQVM 

       730        740        750        760        770        780 
VFVHARNATV RTAMSLIERA KNSGQISCFL PTEGPEYGHA LKQVQKSRNK QVRELFSDGF 

       790        800        810        820        830        840 
SIHHAGMLRQ DRNLVENLFS NGHIKVLVCT ATLAWGVNLP AHAVVIKGTQ IYAAKRGSFV 

       850        860        870        880        890        900 
DLGILDVMQI FGRAGRPQFD KFGEGIIITT HDKLSHYLSL LTQQNPIESQ FLESLADNLN 

       910        920        930        940        950        960 
AEIALGTVTN VEEAVRWMSY TYLYVRMRAN PLAYGISHKA YQIDPTLRKH REQLLIEVGQ 

       970        980        990       1000       1010       1020 
KLDKAKMIRF EERTGYFSST DLGRTASHFY IKYNTIETFN ELFDAHKTEG DIFAIVSKAE 

      1030       1040       1050       1060       1070       1080 
EFDQIKVREE EIEELDALLN NFCELSAPGG VENSYGKINI LLQTYISRGE MDSFSLISDS 

      1090       1100       1110       1120       1130       1140 
AYVAQNAARI VRALFEIALR KRWPTMTYRL LNLSKVIDKR LWGWASPLRQ FSVLPPHILT 

      1150       1160       1170       1180       1190       1200 
RLEEKNLTVD KLKDMRKDEI GHILHHVNIG LKVKQCVHQI PSVTMEASIQ PITRTVLRVS 

      1210       1220       1230       1240       1250       1260 
LNIHPDFSWN DQVHGTVGEP WWIWVEDPTN DHIYHSEYFL ALKKQVINKE AQLLVFTIPI 

      1270       1280       1290       1300       1310       1320 
FEPLPSQYYI RAVSDRWLGA EAVCIINFQH LILPERHPPH TELLDLQPLP ITALGCKAYE 

      1330       1340       1350       1360       1370       1380 
ALYNFSHFNP VQTQIFHTLY HTDCNVLLGA PTGSGKTVAA ELAIFRVFNK YPTSKAVYIA 

      1390       1400       1410       1420       1430       1440 
PLKALVRERM DDWKIRIEEK LGKKVIELTG DVTPDMKSIA KADLIVTTPE KWDGVSRSWQ 

      1450       1460       1470       1480       1490       1500 
NRSYVQQVNI LIIDEIHLLG EERGPVLEVI VSRTNFISSH TEKPVRIVGL STALANARDL 

      1510       1520       1530       1540       1550       1560 
ADWLNIKQMG LFNFRPSVRP VPLEVHIQGF PGQHYCPRMA SMNKPAFQAI RSHSPAKPVL 

      1570       1580       1590       1600       1610       1620 
IFVSSRRQTR LTALELIAFL ATEEDPKQWL NMDEQEMDNI IGTVRDSNLK LTLAFGIGMH 

      1630       1640       1650       1660       1670       1680 
HAGLHERDRK TVEELFVNCK VQVLIATSTL AWGVNFPAHL VIIKGTEYYD GKTRRYVDFP 

      1690       1700       1710       1720       1730       1740 
ITDVLQMMGR AGRPQFDDQG KAVILVHDIK KDFYKKFLYE PFPVESSLLG VLSDHLNAEI 

      1750       1760       1770       1780       1790       1800 
AGGTITSKQD AMDYITWTYF FRRLIMNPSY YSLGDVSQDS INKFLSHLIG QSLVELELSH 

      1810       1820       1830       1840       1850       1860 
CIEVGEDNRT IEPLTCGRIA SYYYLKHKTV KMFKDRLKPE CSTEELLSIL SDAEEYTDLP 

      1870       1880       1890       1900       1910       1920 
VRHNEDHTNN ELAKCLPIEL NPHSFDSPHT KAHLLLQAHL SRAMLPCPDY DTDTKTVLDQ 

      1930       1940       1950       1960       1970       1980 
ALRVCQAMLD VAASQGWLVT VLNITHLIQM VIQGRWLKDS SLLTIPNIEQ HHLHLFRKWK 

      1990       2000       2010       2020       2030       2040 
PPVKSSHAKC RTSIECLPEL IHACEGKDHV FSSMVEKELQ PAKTKQAWNF LSRLPVINVG 

      2050       2060       2070       2080       2090       2100 
ISVKGSWDDL VEGHNELSIS TLTADKRDEN KWIKLHADQE YVLQVSLQRV HFGLPKGKHE 

      2110       2120       2130       2140       2150       2160 
NHAVTPRFPK LKDEGWFLIL GEVDKRELMA VKRVGFVRTH HDASISFFTP ETPGRYIFTL 

      2170       2180       2190 
YLMSDCYLGL DQQYDIYLNV IKANISTKDS DVFTDLSV 

« Hide

Isoform 2 [UniParc].

Checksum: F7057317EB545F77
Show »

FASTA1,128128,982

References

[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Mammary tumor.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-331 AND 2125-2198.
Strain: C57BL/6J.
Tissue: Kidney and Thymus.
[4]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-573, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC137877 Genomic DNA. No translation available.
AC153529 Genomic DNA. No translation available.
AC159114 Genomic DNA. No translation available.
BC032189 mRNA. Translation: AAH32189.1. Different initiation.
BC059917 mRNA. Translation: AAH59917.1.
AK021027 mRNA. Translation: BAC25644.1.
AK052745 mRNA. Translation: BAC35127.1.
RefSeqNP_932124.2. NM_198007.2.
UniGeneMm.222497.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000036726.

Proteomic databases

PRIDEE9PZJ8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000035606; ENSMUSP00000036726; ENSMUSG00000038774. [E9PZJ8-1]
GeneID77987.
KEGGmmu:77987.
UCSCuc007fak.2. mouse. [E9PZJ8-1]

Organism-specific databases

CTD10973.
MGIMGI:1925237. Ascc3.

Phylogenomic databases

GeneTreeENSGT00640000091272.
HOGENOMHOG000157749.
KOK01529.
OMAVHMLHDE.
OrthoDBEOG7PGDPQ.
TreeFamTF105778.

Gene expression databases

BgeeE9PZJ8.

Family and domain databases

Gene3D2.60.40.150. 1 hit.
3.40.50.300. 4 hits.
InterProIPR003593. AAA+_ATPase.
IPR000008. C2_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014756. Ig_E-set.
IPR027417. P-loop_NTPase.
IPR004179. Sec63-dom.
[Graphical view]
PfamPF00270. DEAD. 2 hits.
PF00271. Helicase_C. 2 hits.
PF02889. Sec63. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
SM00487. DEXDc. 2 hits.
SM00490. HELICc. 2 hits.
SM00611. SEC63. 2 hits.
SM00973. Sec63. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 4 hits.
SSF81296. SSF81296. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio347945.
PROE9PZJ8.
SOURCESearch...

Entry information

Entry nameASCC3_MOUSE
AccessionPrimary (citable) accession number: E9PZJ8
Secondary accession number(s): Q6PB36 expand/collapse secondary AC list , Q8C1G1, Q8C707, Q8K292
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: April 5, 2011
Last modified: April 16, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot