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E9PZJ8

- ASCC3_MOUSE

UniProt

E9PZJ8 - ASCC3_MOUSE

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Protein
Activating signal cointegrator 1 complex subunit 3
Gene
Ascc3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3, enabling ALKHB3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions. Enhances NF-kappa-B, SRF and AP1 transactivation By similarity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi500 – 5078ATP By similarity
Nucleotide bindingi1350 – 13578ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent 3'-5' DNA helicase activity Source: UniProtKB
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA dealkylation involved in DNA repair Source: UniProtKB
  2. DNA duplex unwinding Source: UniProtKB
  3. cell proliferation Source: UniProtKB
  4. regulation of transcription, DNA-templated Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Activating signal cointegrator 1 complex subunit 3 (EC:3.6.4.12)
Gene namesi
Name:Ascc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1925237. Ascc3.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity

GO - Cellular componenti

  1. Golgi apparatus Source: Ensembl
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21982198Activating signal cointegrator 1 complex subunit 3
PRO_0000416915Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei573 – 5731N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiE9PZJ8.
PRIDEiE9PZJ8.

Expressioni

Gene expression databases

BgeeiE9PZJ8.

Interactioni

Subunit structurei

Part of TRIP4 complex, that contains ASCC1, ASCC2 and ASCC3. The TRIP4 complex interacts with ALKBH3 By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036726.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini487 – 670184Helicase ATP-binding 1
Add
BLAST
Domaini697 – 915219Helicase C-terminal 1
Add
BLAST
Domaini979 – 1288310SEC63 1
Add
BLAST
Domaini1337 – 1512176Helicase ATP-binding 2
Add
BLAST
Domaini1545 – 1740196Helicase C-terminal 2
Add
BLAST
Domaini1813 – 2177365SEC63 2
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili18 – 8164 Reviewed prediction
Add
BLAST
Coiled coili328 – 35629 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi612 – 6154DEVH box
Motifi1454 – 14574DEIH box

Sequence similaritiesi

Belongs to the helicase family.
Contains 2 SEC63 domains.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

GeneTreeiENSGT00640000091272.
HOGENOMiHOG000157749.
KOiK01529.
OMAiVHMLHDE.
OrthoDBiEOG7PGDPQ.
TreeFamiTF105778.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR003593. AAA+_ATPase.
IPR000008. C2_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014756. Ig_E-set.
IPR027417. P-loop_NTPase.
IPR004179. Sec63-dom.
[Graphical view]
PfamiPF00270. DEAD. 2 hits.
PF00271. Helicase_C. 2 hits.
PF02889. Sec63. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM00487. DEXDc. 2 hits.
SM00490. HELICc. 2 hits.
SM00611. SEC63. 2 hits.
SM00973. Sec63. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: E9PZJ8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MALPRLTGAL RSFSNVTKQD NYNEEVADLK LKRSKLHEQV LDFGLTWKKI     50
VKFLNEKLEK NKMQNINEDL KDILQAAKQI VGTDNGREAI ESGAAFLFMT 100
FHMTDSVGYM ETKAIRQTFG PFPSSSATSA CNATNRIISH FSQDDLTAFV 150
QMAENPCNDR VVFGKNLAFS FDMYDLDHFD ELPINGESQK TISLDYKKFL 200
NEQFQEPYTP ELKPVEKTNG SLLWCEVEKY LNATLKEMTE AARVEDLCCT 250
LYDMLASAKS GDELQDELFE LLGPEGLDLI EKLLQNRITI VDRFLNSSSD 300
HKFQVLQDSC KKILGENSKP NYGCQVTIQS EQEKQLMKQY RREEKRIARR 350
EKKAGEDGEV SGEGVLPFDP KELRIQREHA LLNARNAPIL GRQRDVEFEK 400
IRYPHVYDSQ AQARETSAFI AGAKMILPEG IQRENTKLYE EVRIPYGEPM 450
PVGFEEKPVY IKDLDEVGQL AFKGMKRLNR IQSIVFETAY NTNENMLICA 500
PTGAGKTNIA MLTILHEIRQ HFHQGVIKKN EFKIVYVAPM KALAAEMTNY 550
FSKRLEPLGI VVKELTGDMQ LSKSEILRTQ MLVTTPEKWD VVTRKSVGDV 600
ALSQIVKLLI LDEVHLLHED RGPVLESIVA RTLRQVESTQ SMIRILGLSA 650
TLPNYLDVAT FLHVNPYIGL FYFDGRFRPV PLGQTFLGIK STNKMQQLNN 700
MDEVCYESVL KQVKAGHQVM VFVHARNATV RTAMSLIERA KNSGQISCFL 750
PTEGPEYGHA LKQVQKSRNK QVRELFSDGF SIHHAGMLRQ DRNLVENLFS 800
NGHIKVLVCT ATLAWGVNLP AHAVVIKGTQ IYAAKRGSFV DLGILDVMQI 850
FGRAGRPQFD KFGEGIIITT HDKLSHYLSL LTQQNPIESQ FLESLADNLN 900
AEIALGTVTN VEEAVRWMSY TYLYVRMRAN PLAYGISHKA YQIDPTLRKH 950
REQLLIEVGQ KLDKAKMIRF EERTGYFSST DLGRTASHFY IKYNTIETFN 1000
ELFDAHKTEG DIFAIVSKAE EFDQIKVREE EIEELDALLN NFCELSAPGG 1050
VENSYGKINI LLQTYISRGE MDSFSLISDS AYVAQNAARI VRALFEIALR 1100
KRWPTMTYRL LNLSKVIDKR LWGWASPLRQ FSVLPPHILT RLEEKNLTVD 1150
KLKDMRKDEI GHILHHVNIG LKVKQCVHQI PSVTMEASIQ PITRTVLRVS 1200
LNIHPDFSWN DQVHGTVGEP WWIWVEDPTN DHIYHSEYFL ALKKQVINKE 1250
AQLLVFTIPI FEPLPSQYYI RAVSDRWLGA EAVCIINFQH LILPERHPPH 1300
TELLDLQPLP ITALGCKAYE ALYNFSHFNP VQTQIFHTLY HTDCNVLLGA 1350
PTGSGKTVAA ELAIFRVFNK YPTSKAVYIA PLKALVRERM DDWKIRIEEK 1400
LGKKVIELTG DVTPDMKSIA KADLIVTTPE KWDGVSRSWQ NRSYVQQVNI 1450
LIIDEIHLLG EERGPVLEVI VSRTNFISSH TEKPVRIVGL STALANARDL 1500
ADWLNIKQMG LFNFRPSVRP VPLEVHIQGF PGQHYCPRMA SMNKPAFQAI 1550
RSHSPAKPVL IFVSSRRQTR LTALELIAFL ATEEDPKQWL NMDEQEMDNI 1600
IGTVRDSNLK LTLAFGIGMH HAGLHERDRK TVEELFVNCK VQVLIATSTL 1650
AWGVNFPAHL VIIKGTEYYD GKTRRYVDFP ITDVLQMMGR AGRPQFDDQG 1700
KAVILVHDIK KDFYKKFLYE PFPVESSLLG VLSDHLNAEI AGGTITSKQD 1750
AMDYITWTYF FRRLIMNPSY YSLGDVSQDS INKFLSHLIG QSLVELELSH 1800
CIEVGEDNRT IEPLTCGRIA SYYYLKHKTV KMFKDRLKPE CSTEELLSIL 1850
SDAEEYTDLP VRHNEDHTNN ELAKCLPIEL NPHSFDSPHT KAHLLLQAHL 1900
SRAMLPCPDY DTDTKTVLDQ ALRVCQAMLD VAASQGWLVT VLNITHLIQM 1950
VIQGRWLKDS SLLTIPNIEQ HHLHLFRKWK PPVKSSHAKC RTSIECLPEL 2000
IHACEGKDHV FSSMVEKELQ PAKTKQAWNF LSRLPVINVG ISVKGSWDDL 2050
VEGHNELSIS TLTADKRDEN KWIKLHADQE YVLQVSLQRV HFGLPKGKHE 2100
NHAVTPRFPK LKDEGWFLIL GEVDKRELMA VKRVGFVRTH HDASISFFTP 2150
ETPGRYIFTL YLMSDCYLGL DQQYDIYLNV IKANISTKDS DVFTDLSV 2198
Length:2,198
Mass (Da):250,557
Last modified:April 5, 2011 - v1
Checksum:i39FF851ED47394FD
GO
Isoform 2 (identifier: E9PZJ8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1070: Missing.

Note: No experimental confirmation available.

Show »
Length:1,128
Mass (Da):128,982
Checksum:iF7057317EB545F77
GO

Sequence cautioni

The sequence AAH32189.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 10701070Missing in isoform 2.
VSP_042999Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1818 – 18181R → L in AAH59917. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC137877 Genomic DNA. No translation available.
AC153529 Genomic DNA. No translation available.
AC159114 Genomic DNA. No translation available.
BC032189 mRNA. Translation: AAH32189.1. Different initiation.
BC059917 mRNA. Translation: AAH59917.1.
AK021027 mRNA. Translation: BAC25644.1.
AK052745 mRNA. Translation: BAC35127.1.
CCDSiCCDS48555.1. [E9PZJ8-1]
RefSeqiNP_932124.2. NM_198007.2. [E9PZJ8-1]
UniGeneiMm.222497.

Genome annotation databases

EnsembliENSMUST00000035606; ENSMUSP00000036726; ENSMUSG00000038774. [E9PZJ8-1]
GeneIDi77987.
KEGGimmu:77987.
UCSCiuc007fak.2. mouse. [E9PZJ8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC137877 Genomic DNA. No translation available.
AC153529 Genomic DNA. No translation available.
AC159114 Genomic DNA. No translation available.
BC032189 mRNA. Translation: AAH32189.1 . Different initiation.
BC059917 mRNA. Translation: AAH59917.1 .
AK021027 mRNA. Translation: BAC25644.1 .
AK052745 mRNA. Translation: BAC35127.1 .
CCDSi CCDS48555.1. [E9PZJ8-1 ]
RefSeqi NP_932124.2. NM_198007.2. [E9PZJ8-1 ]
UniGenei Mm.222497.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000036726.

Proteomic databases

MaxQBi E9PZJ8.
PRIDEi E9PZJ8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000035606 ; ENSMUSP00000036726 ; ENSMUSG00000038774 . [E9PZJ8-1 ]
GeneIDi 77987.
KEGGi mmu:77987.
UCSCi uc007fak.2. mouse. [E9PZJ8-1 ]

Organism-specific databases

CTDi 10973.
MGIi MGI:1925237. Ascc3.

Phylogenomic databases

GeneTreei ENSGT00640000091272.
HOGENOMi HOG000157749.
KOi K01529.
OMAi VHMLHDE.
OrthoDBi EOG7PGDPQ.
TreeFami TF105778.

Miscellaneous databases

NextBioi 347945.
PROi E9PZJ8.
SOURCEi Search...

Gene expression databases

Bgeei E9PZJ8.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
3.40.50.300. 4 hits.
InterProi IPR003593. AAA+_ATPase.
IPR000008. C2_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014756. Ig_E-set.
IPR027417. P-loop_NTPase.
IPR004179. Sec63-dom.
[Graphical view ]
Pfami PF00270. DEAD. 2 hits.
PF00271. Helicase_C. 2 hits.
PF02889. Sec63. 2 hits.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
SM00487. DEXDc. 2 hits.
SM00490. HELICc. 2 hits.
SM00611. SEC63. 2 hits.
SM00973. Sec63. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 4 hits.
SSF81296. SSF81296. 1 hit.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Mammary tumor.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-331 AND 2125-2198.
    Strain: C57BL/6J.
    Tissue: Kidney and Thymus.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-573, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiASCC3_MOUSE
AccessioniPrimary (citable) accession number: E9PZJ8
Secondary accession number(s): Q6PB36
, Q8C1G1, Q8C707, Q8K292
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: April 5, 2011
Last modified: July 9, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi