ID   S28A1_MOUSE             Reviewed;         648 AA.
AC   E9PXX9; Q6P8I9;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   05-APR-2011, sequence version 1.
DT   27-MAR-2024, entry version 95.
DE   RecName: Full=Sodium/nucleoside cotransporter 1 {ECO:0000250|UniProtKB:O00337};
DE   AltName: Full=Concentrative nucleoside transporter 1;
DE            Short=CNT 1;
DE   AltName: Full=Na(+)/nucleoside cotransporter 1;
DE   AltName: Full=Sodium-coupled nucleoside transporter 1;
DE   AltName: Full=Solute carrier family 28 member 1 {ECO:0000312|MGI:MGI:3605073};
GN   Name=Slc28a1 {ECO:0000312|MGI:MGI:3605073};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Sodium and pyrimidine nucleoside symporter of the plasma
CC       membrane that imports uridine, thymidine and cytidine into cells by
CC       coupling their transport to the transmembrane sodium electrochemical
CC       gradient. Also transports adenosine, an atypical substrate transported
CC       with high apparent affinity, but low maximum velocity. Therefore,
CC       exhibits the transport characteristics of the nucleoside transport
CC       system cit or N2 subtype (N2/cit). Involved in renal nucleoside
CC       (re)absorption. {ECO:0000250|UniProtKB:O00337}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Na(+)(out) + uridine(out) = Na(+)(in) + uridine(in);
CC         Xref=Rhea:RHEA:69887, ChEBI:CHEBI:16704, ChEBI:CHEBI:29101;
CC         Evidence={ECO:0000250|UniProtKB:O00337};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Na(+)(out) + thymidine(out) = Na(+)(in) + thymidine(in);
CC         Xref=Rhea:RHEA:69891, ChEBI:CHEBI:17748, ChEBI:CHEBI:29101;
CC         Evidence={ECO:0000250|UniProtKB:O00337};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cytidine(out) + Na(+)(out) = cytidine(in) + Na(+)(in);
CC         Xref=Rhea:RHEA:69895, ChEBI:CHEBI:17562, ChEBI:CHEBI:29101;
CC         Evidence={ECO:0000250|UniProtKB:O00337};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(out) + Na(+)(out) = adenosine(in) + Na(+)(in);
CC         Xref=Rhea:RHEA:69927, ChEBI:CHEBI:16335, ChEBI:CHEBI:29101;
CC         Evidence={ECO:0000250|UniProtKB:O00337};
CC   -!- ACTIVITY REGULATION: Due to its high apparent affinity but slow
CC       transport, adenosine could act as a negative regulator of pyrimidine
CC       transport under some conditions. {ECO:0000250|UniProtKB:O00337}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:O00337};
CC       Multi-pass membrane protein {ECO:0000250|UniProtKB:Q62674}. Apical cell
CC       membrane {ECO:0000250|UniProtKB:O00337}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q62674}.
CC   -!- PTM: N-glycosylated. N-glycosylation is required for localization to
CC       the plasma membrane and the transporter activity.
CC       {ECO:0000250|UniProtKB:O00337}.
CC   -!- SIMILARITY: Belongs to the concentrative nucleoside transporter (CNT)
CC       (TC 2.A.41) family. {ECO:0000305}.
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DR   EMBL; BC061230; AAH61230.1; -; mRNA.
DR   CCDS; CCDS21402.1; -.
DR   RefSeq; NP_001004184.2; NM_001004184.3.
DR   RefSeq; XP_006541039.1; XM_006540976.3.
DR   RefSeq; XP_006541040.1; XM_006540977.3.
DR   RefSeq; XP_006541041.1; XM_006540978.3.
DR   RefSeq; XP_006541042.1; XM_006540979.3.
DR   AlphaFoldDB; E9PXX9; -.
DR   SMR; E9PXX9; -.
DR   STRING; 10090.ENSMUSP00000112421; -.
DR   GlyCosmos; E9PXX9; 3 sites, No reported glycans.
DR   GlyGen; E9PXX9; 3 sites.
DR   PhosphoSitePlus; E9PXX9; -.
DR   PaxDb; 10090-ENSMUSP00000026820; -.
DR   ProteomicsDB; 339181; -.
DR   Antibodypedia; 65918; 80 antibodies from 19 providers.
DR   DNASU; 434203; -.
DR   Ensembl; ENSMUST00000026820.11; ENSMUSP00000026820.5; ENSMUSG00000025726.12.
DR   Ensembl; ENSMUST00000119083.2; ENSMUSP00000112421.2; ENSMUSG00000025726.12.
DR   GeneID; 434203; -.
DR   KEGG; mmu:434203; -.
DR   UCSC; uc009ibs.1; mouse.
DR   AGR; MGI:3605073; -.
DR   CTD; 9154; -.
DR   MGI; MGI:3605073; Slc28a1.
DR   VEuPathDB; HostDB:ENSMUSG00000025726; -.
DR   eggNOG; KOG3747; Eukaryota.
DR   GeneTree; ENSGT00390000016025; -.
DR   HOGENOM; CLU_016813_3_2_1; -.
DR   InParanoid; E9PXX9; -.
DR   OMA; IVWHTVI; -.
DR   OrthoDB; 1333063at2759; -.
DR   PhylomeDB; E9PXX9; -.
DR   TreeFam; TF314131; -.
DR   Reactome; R-MMU-83936; Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.
DR   BioGRID-ORCS; 434203; 3 hits in 76 CRISPR screens.
DR   ChiTaRS; Slc28a1; mouse.
DR   PRO; PR:E9PXX9; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   RNAct; E9PXX9; Protein.
DR   Bgee; ENSMUSG00000025726; Expressed in proximal tubule and 27 other cell types or tissues.
DR   GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR   GO; GO:0031526; C:brush border membrane; IDA:ARUK-UCL.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:1901474; F:azole transmembrane transporter activity; ISO:MGI.
DR   GO; GO:0005350; F:pyrimidine nucleobase transmembrane transporter activity; ISO:MGI.
DR   GO; GO:0015389; F:pyrimidine- and adenosine-specific:sodium symporter activity; ISS:UniProtKB.
DR   GO; GO:0015213; F:uridine transmembrane transporter activity; ISO:MGI.
DR   GO; GO:0045117; P:azole transmembrane transport; ISO:MGI.
DR   GO; GO:0015861; P:cytidine transport; ISO:MGI.
DR   GO; GO:0180015; P:nucleoside import across plasma membrane; ISS:UniProtKB.
DR   GO; GO:1901642; P:nucleoside transmembrane transport; ISO:MGI.
DR   GO; GO:0015855; P:pyrimidine nucleobase transport; ISO:MGI.
DR   GO; GO:0072531; P:pyrimidine-containing compound transmembrane transport; ISO:MGI.
DR   GO; GO:0015862; P:uridine transport; ISO:MGI.
DR   InterPro; IPR008276; C_nuclsd_transpt.
DR   InterPro; IPR018270; C_nuclsd_transpt_met_bac.
DR   InterPro; IPR011657; CNT_C_dom.
DR   InterPro; IPR002668; CNT_N_dom.
DR   InterPro; IPR011642; Gate_dom.
DR   NCBIfam; TIGR00804; nupC; 1.
DR   PANTHER; PTHR10590; SODIUM/NUCLEOSIDE COTRANSPORTER; 1.
DR   PANTHER; PTHR10590:SF16; SODIUM_NUCLEOSIDE COTRANSPORTER 1; 1.
DR   Pfam; PF07670; Gate; 1.
DR   Pfam; PF07662; Nucleos_tra2_C; 1.
DR   Pfam; PF01773; Nucleos_tra2_N; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Glycoprotein; Membrane; Reference proteome; Symport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..648
FT                   /note="Sodium/nucleoside cotransporter 1"
FT                   /id="PRO_0000455292"
FT   TOPO_DOM        1..83
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        84..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        105..108
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        130..147
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        169..175
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        197..201
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        202..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        223..265
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        266..286
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        287..294
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        295..318
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        319..339
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        340..360
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        361
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        362..380
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        381..427
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        428..448
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        449..470
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        471..491
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        492..531
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        532..552
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        553..571
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   TRANSMEM        572..592
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        593..648
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:Q62674"
FT   CARBOHYD        605
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        625
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        643
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        96
FT                   /note="G -> S (in Ref. 2; AAH61230)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        137
FT                   /note="K -> E (in Ref. 2; AAH61230)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   648 AA;  71174 MW;  C32DCEA9133147E1 CRC64;
     MADDTPRQRE SISLTPVAHG LENMGAEFLE IMEEGQLPHR HSSLPEGGGS RSKAVWKPFS
     RWRSLQPTVQ ARSLCREHWQ LFEWISKGLL STAYIGFLIV ACLLDFPRAL ALFVITCVVL
     VFLAYNLLKR LLGSKLKKCV KFQGHSCLSL WLKRGLALAA GLGVILWLSL DTAQRPEQLV
     SFAGICVFLV LLFAGSKHHR AVSWRAVSWG LGLQFVLGLF VIRTEPGFVA FQWLGDQIRV
     FLSYTEAGSS FVFGEALVKD VFAFQVLPII VFFSCVMSVL YYLGLMQWVI LKIAWLMQVT
     MGTSATETLS VAGNIFVSQT EAPLLIRPYL ADMTLSEVHV VMTGGYATIA GSLLGAYISF
     GIDASSLIAA SVMAAPCALA LSKLVYPEVE ESKFRSEEGV KLTYGDAQNL VEAASAGAAI
     SVKVVANIAA NLIAFLAVLA FINAALSWLG DMVDIQGLSF QLICSYVLRP VAFLMGVAWE
     DCPVVAELLG IKLFLNEFVA YQELSQYKQR RLAGAEEWLG DKKQWISVRA EILTTYALCG
     FANFSSIGIM LGGLTSMVPQ RRSDFSQIVL RALITGAFVS LVNACVAGIL YVPRGVEVDC
     MSLLNQTVSS SSFEVYLCCR QVFQNTSLEF GQEALHNCCR FYNHTVCT
//