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Protein

Proliferation marker protein Ki-67

Gene

Mki67

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.By similarityCurated2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi2973 – 2980ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • animal organ regeneration Source: Ensembl
  • cell proliferation Source: MGI
  • cellular response to heat Source: Ensembl
  • cholangiocyte proliferation Source: MGI
  • DNA metabolic process Source: Ensembl
  • hepatocyte proliferation Source: MGI
  • hyaluronan metabolic process Source: Ensembl
  • meiotic nuclear division Source: MGI
  • regulation of chromatin organization Source: UniProtKB
  • regulation of chromosome segregation Source: UniProtKB
  • regulation of mitotic nuclear division Source: UniProtKB
  • response to organic cyclic compound Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Proliferation marker protein Ki-67Curated
Alternative name(s):
Antigen identified by monoclonal antibody Ki-67 homologCurated
Short name:
Antigen KI-67 homologCurated
Short name:
Antigen Ki67 homologCurated
Gene namesi
Name:Mki67Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:106035. Mki67.

Subcellular locationi

  • Chromosome 2 Publications
  • Nucleus 2 Publications
  • Nucleusnucleolus 2 Publications

  • Note: Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:8834799, PubMed:12355204). Associates with satellite DNA in G1 phase (By similarity). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (By similarity). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (By similarity).By similarity2 Publications

GO - Cellular componenti

  • chromosome, centromeric region Source: MGI
  • condensed chromosome Source: MGI
  • cytoplasm Source: MGI
  • membrane Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice were born at the expected Mendelian ratio and show no overt phenotype (PubMed:26949251). Cells do not show proliferation defects, but chromatin organization is impaired, with defects in heterochromatin compaction and long-range genomic interactions (PubMed:26949251).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004375351 – 3177Proliferation marker protein Ki-67Add BLAST3177

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei125PhosphoserineBy similarity1
Modified residuei128PhosphoserineBy similarity1
Modified residuei162PhosphoserineCombined sources1
Modified residuei250PhosphoserineBy similarity1
Modified residuei276PhosphoserineCombined sources1
Modified residuei277PhosphoserineCombined sources1
Modified residuei286PhosphoserineCombined sources1
Modified residuei287PhosphoserineCombined sources1
Modified residuei307PhosphothreonineBy similarity1
Modified residuei316PhosphothreonineBy similarity1
Modified residuei321PhosphoserineBy similarity1
Modified residuei337PhosphoserineCombined sources1
Modified residuei373PhosphoserineCombined sources1
Modified residuei498PhosphoserineBy similarity1
Modified residuei503PhosphoserineCombined sources1
Modified residuei588PhosphoserineCombined sources1
Modified residuei701PhosphothreonineBy similarity1
Cross-linki1026Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1062PhosphoserineBy similarity1
Cross-linki1082Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei1114PhosphoserineBy similarity1
Modified residuei1122PhosphothreonineBy similarity1
Modified residuei1125PhosphoserineBy similarity1
Modified residuei1150PhosphothreonineCombined sources1
Modified residuei1152PhosphoserineCombined sources1
Modified residuei1159PhosphothreonineCombined sources1
Modified residuei1175PhosphothreonineCombined sources1
Modified residuei1189PhosphoserineBy similarity1
Modified residuei1215PhosphothreonineBy similarity1
Modified residuei1235PhosphoserineBy similarity1
Modified residuei1243PhosphothreonineBy similarity1
Modified residuei1279PhosphothreonineBy similarity1
Modified residuei1295PhosphothreonineBy similarity1
Modified residuei1307PhosphothreonineBy similarity1
Modified residuei1315PhosphothreonineBy similarity1
Modified residuei1335PhosphothreonineBy similarity1
Modified residuei1356PhosphoserineBy similarity1
Modified residuei1363PhosphothreonineCombined sources1
Modified residuei1366PhosphoserineCombined sources1
Modified residuei1400PhosphothreonineCombined sources1
Modified residuei1416PhosphothreonineCombined sources1
Modified residuei1469PhosphoserineCombined sources1
Modified residuei1477PhosphothreonineBy similarity1
Modified residuei1480PhosphoserineBy similarity1
Modified residuei1513PhosphothreonineBy similarity1
Modified residuei1542PhosphoserineBy similarity1
Modified residuei1587PhosphoserineCombined sources1
Modified residuei1609N6-acetyllysineBy similarity1
Modified residuei1684PhosphothreonineBy similarity1
Modified residuei1712PhosphothreonineBy similarity1
Modified residuei1734PhosphoserineCombined sources1
Modified residuei1766PhosphothreonineBy similarity1
Modified residuei1779PhosphoserineBy similarity1
Modified residuei1805PhosphothreonineBy similarity1
Modified residuei1825PhosphoserineCombined sources1
Modified residuei1859PhosphothreonineBy similarity1
Modified residuei1868PhosphothreonineCombined sources1
Modified residuei1884PhosphothreonineCombined sources1
Modified residuei1924PhosphothreonineBy similarity1
Modified residuei1944PhosphoserineBy similarity1
Modified residuei1966N6-acetyllysineBy similarity1
Modified residuei1989PhosphothreonineCombined sources1
Modified residuei2005PhosphothreonineCombined sources1
Modified residuei2025PhosphothreonineBy similarity1
Cross-linki2027Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei2045PhosphothreonineBy similarity1
Modified residuei2065PhosphoserineBy similarity1
Modified residuei2073PhosphothreonineCombined sources1
Modified residuei2076PhosphoserineCombined sources1
Modified residuei2095PhosphoserineBy similarity1
Modified residuei2103PhosphoserineCombined sources1
Modified residuei2106PhosphothreonineCombined sources1
Modified residuei2122PhosphothreonineCombined sources1
Modified residuei2162PhosphothreonineBy similarity1
Modified residuei2182PhosphoserineBy similarity1
Modified residuei2190PhosphothreonineBy similarity1
Modified residuei2198PhosphoserineBy similarity1
Modified residuei2218PhosphothreonineCombined sources1
Modified residuei2220PhosphoserineCombined sources1
Modified residuei2227PhosphothreonineCombined sources1
Modified residuei2243PhosphothreonineCombined sources1
Modified residuei2283PhosphothreonineBy similarity1
Modified residuei2303PhosphoserineBy similarity1
Modified residuei2311PhosphothreonineBy similarity1
Modified residuei2348PhosphothreonineBy similarity1
Modified residuei2390PhosphoserineCombined sources1
Modified residuei2392PhosphoserineCombined sources1
Modified residuei2405PhosphothreonineBy similarity1
Modified residuei2423PhosphoserineCombined sources1
Modified residuei2425PhosphoserineCombined sources1
Cross-linki2451Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei2464PhosphoserineBy similarity1
Modified residuei2487PhosphoserineBy similarity1
Modified residuei2545PhosphoserineCombined sources1
Modified residuei2592PhosphoserineBy similarity1
Modified residuei2649PhosphoserineBy similarity1
Cross-linki2675Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki2675Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei2768PhosphoserineBy similarity1
Modified residuei2780PhosphoserineCombined sources1
Modified residuei2928N6-acetyllysineCombined sources1
Modified residuei2980PhosphoserineBy similarity1
Modified residuei3021PhosphothreonineCombined sources1
Modified residuei3061PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiE9PVX6.
PaxDbiE9PVX6.
PeptideAtlasiE9PVX6.
PRIDEiE9PVX6.

PTM databases

iPTMnetiE9PVX6.
PhosphoSitePlusiE9PVX6.

Expressioni

Tissue specificityi

Mainly present in proliferating cells (at protein level).1 Publication

Developmental stagei

Accumulates during the late G1 stage in the nucleus and maximum expression is found during G2 phase and mitosis (PubMed:8834799). During male meiosis, present in nuclei of all stages from the spermatogonium through spermatocytes I and II up to the earliest spermatid stage (early round spermatids) and then fades out (PubMed:12355204). Not detected in later spermatid stages or sperm (PubMed:12355204). During female meiosis, present in prophase I oocytes of fetal ovaries, while it is absent in resting oocytes. Reappears in oocytes of growing follicles and is continuously present up to metaphase II (at protein level) (PubMed:12355204).2 Publications

Gene expression databases

BgeeiENSMUSG00000031004.
GenevisibleiE9PVX6. MM.

Interactioni

Subunit structurei

Interacts with KIF15. Interacts (via the FHA domain) with NIFK. Interacts with PPP1CC. Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity.By similarity

GO - Molecular functioni

Protein-protein interaction databases

MINTiMINT-4109797.
STRINGi10090.ENSMUSP00000033310.

Structurei

3D structure databases

ProteinModelPortaliE9PVX6.
SMRiE9PVX6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 76FHAPROSITE-ProRule annotationAdd BLAST50
Domaini462 – 509PP1-bindingSequence analysisAdd BLAST48
Repeati994 – 1101K167R 1Sequence analysisAdd BLAST108
Repeati1108 – 1216K167R 2Sequence analysisAdd BLAST109
Repeati1228 – 1336K167R 3Sequence analysisAdd BLAST109
Repeati1348 – 1450K167R 4Sequence analysisAdd BLAST103
Repeati1461 – 1569K167R 5Sequence analysisAdd BLAST109
Repeati1582 – 1684K167R 6Sequence analysisAdd BLAST103
Repeati1696 – 1806K167R 7Sequence analysisAdd BLAST111
Repeati1817 – 1925K167R 8Sequence analysisAdd BLAST109
Repeati1937 – 2046K167R 9Sequence analysisAdd BLAST110
Repeati2059 – 2163K167R 10Sequence analysisAdd BLAST105
Repeati2175 – 2284K167R 11Sequence analysisAdd BLAST110
Repeati2296 – 2405K167R 12Sequence analysisAdd BLAST110
Repeati2419 – 2526K167R 13Sequence analysisAdd BLAST108
Repeati2643 – 2748K167R 15Sequence analysisAdd BLAST106
Repeati2762 – 2870K167R 16Sequence analysisAdd BLAST109

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation
Contains 16 K167R repeats.Curated
Contains 1 PP1-binding domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410JR7C. Eukaryota.
ENOG410ZXYW. LUCA.
GeneTreeiENSGT00530000064425.
HOGENOMiHOG000113223.
HOVERGENiHBG006213.
InParanoidiE9PVX6.
KOiK17582.
OMAiTGLSKVD.
OrthoDBiEOG091G005S.
TreeFamiTF336000.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR012568. K167R.
IPR029334. PP1-bd.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
PF08065. K167R. 17 hits.
PF15276. PP1_bind. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
SM01295. K167R. 16 hits.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: E9PVX6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSAHLVTI KRSGDDGAHF PLSLSSCLFG RSIECDIRIQ LPVVSKRHCK
60 70 80 90 100
IEVKEQEAIL YNFSSTNPTQ VNGVTIDEPV RLRHGDIITI IDRSFRYEDG
110 120 130 140 150
NHEDGSKPTE FPGKSLGKEP SRRASRDSFC ADPDGEGQDT KASKMTASRR
160 170 180 190 200
SFVYAKGLSA DSPASDGSKN SVSQDSSGHV EQHTGRNIVE PTSGDLFKKS
210 220 230 240 250
RSTGSSYREP KSSPTQSLSN SNEKESPFEK LYQSMKEELD VKSQKSCRKS
260 270 280 290 300
EPQPDRAAEE SRETQLLVSG RARAKSSGST PVTAASSPKV GKIWTERWRG
310 320 330 340 350
GMVPVQTSTE TAKMKTPVRH SQQLKDEDSR VTGRRHSVNL DEGESAQAVH
360 370 380 390 400
KTVTPGKLAT RNQAAVEAGD VASPADTPEH SSSKKRSIPA KVEAPSAETQ
410 420 430 440 450
KRLSLTQRLV PGEKKTPKGS FSKPEKLATA AEQTCSGLPG LSSVDISNFG
460 470 480 490 500
DSINKSEGMP MKRRRVSFGG HLRPELFDEN LPPNTPLKRG ETPTKRKSLG
510 520 530 540 550
THSPAVLKTI IKERPQSPGK QESPGITPPR TNDQRRRSGR TSSGSKFLCE
560 570 580 590 600
TDIPKKAGRK SGNLPAKRAS ISRSQHGILQ MICSKRRSGA SEANLIVAKS
610 620 630 640 650
WADVVKLGVK QTQTKVAKHV PQKQTSKRQR RPSTPKKPTS NLHNQFTTGH
660 670 680 690 700
ANSPCTIVVG RAQIEKVSVP ARPYKMLNNL MLNRKVDFSE DLSGLTEMFK
710 720 730 740 750
TPVKEKQQQM SDTGSVLSNS ANLSERQLQV TNSGDIPEPI TTEILGEKVL
760 770 780 790 800
SSTRNAAKQQ SDRYSASPTL RRRSIKHENT VQTPKNVHNI TDLEKKTPVS
810 820 830 840 850
ETEPLKTASS VSKLRRSREL RHTLVETMNE KTEAVLAENT TARHLRGTFR
860 870 880 890 900
EQKVDQQVQD NENAPQRCKE SGELSEGSEK TSARRSSARK QKPTKDLLGS
910 920 930 940 950
QMVTQTADYA EELLSQGQGT IQNLEESMHM QNTSISEDQG ITEKKVNIIV
960 970 980 990 1000
YATKEKHSPK TPGKKAQPLE GPAGLKEHFE TPNPKDKPIT EDRTRVLCKS
1010 1020 1030 1040 1050
PQVTTENITT NTKPQTSTSG KKVDMKEESS ALTKRIHMPG ESRHNPKILK
1060 1070 1080 1090 1100
LECEDIKALK QSENEMLTST VNGSKRTLEK SKKKAQPLED LTCFQELFIS
1110 1120 1130 1140 1150
PVPTNIIKKI PSKSPHTQPV RTPASTKRLS KTGLSKVDVR QEPSTLGKRT
1160 1170 1180 1190 1200
KSPGRAPGTP APVQEENDST AFMETPKQKL DFAGNSSGSK RRSRTSKNRS
1210 1220 1230 1240 1250
QPLEDLDGFQ ELFQTPAGAS DSVTVEESAK ISLESSQAEP VKTPASTKRR
1260 1270 1280 1290 1300
SKMSLMKVDM KELSILEKQT QSRGRDAGTP APMQEGNGTT AIMETPKQKL
1310 1320 1330 1340 1350
DFTGNSTGHK RRPRTPKIRA QPLEDLDGFQ ELFQTPAGAN DSVTVEESAK
1360 1370 1380 1390 1400
MSLESSQAEP VKTPASTKRL SKTDLSKVDV REDPSILGKK TKSPGRAPGT
1410 1420 1430 1440 1450
PAPVQEENDC TAYMETPKQK LESIENLTGL RKQSRTPKDI TGFQDSFQIP
1460 1470 1480 1490 1500
DHANGPLVVV KTKKMFFNSP QPESAITRKS RERQSRASIS KIDVKEELLE
1510 1520 1530 1540 1550
SEEHLQLGEG VDTFQVSTNK VIRSSRKPAK RKLDSTAGMP NSKRMRCSSK
1560 1570 1580 1590 1600
DNTPCLEDLN GFQELFQMPG YANDSLTTGI STMLARSPQL GPVRTQINKK
1610 1620 1630 1640 1650
SLPKIILRKM DVTEEISGLW KQSLGRVHTT QEQEDNAIKA IMEIPKETLQ
1660 1670 1680 1690 1700
TAADGTRLTR QPQTPKEKVQ PLEDHSVFQE LFQTSRYCSD PLIGNKQTRM
1710 1720 1730 1740 1750
SLRSPQPGFV RTPRTSKRLA KTSVGNIAVR EKISPVSLPQ CATGEVVHIP
1760 1770 1780 1790 1800
IGPEDDTENK GVKESTPQTL DSSASRTVSK RQQGAHEERP QFSGDLFHPQ
1810 1820 1830 1840 1850
ELFQTPASGK DPVTVDETTK IALQSPQPGH IINPASMKRQ SNMSLRKDMR
1860 1870 1880 1890 1900
EFSILEKQTQ SRGRDAGTPA PMQEENGTTA IMETPKQKLD FIGNSTGHKR
1910 1920 1930 1940 1950
RPRTPKNRAQ PLEDLDGFQE LFQTPAGASD PVSVEESAKI SLASSQAEPV
1960 1970 1980 1990 2000
RTPASTKRRS KTGLSKVDVR QEPSTLGKRM KSLGRAPGTP APVQEENDST
2010 2020 2030 2040 2050
AFMETPKQKL DFTGNSSGHK RRPQTPKIRA QPLEDLDGFQ ELFQTPAGAN
2060 2070 2080 2090 2100
DSVTVEESVK MSLESSQAEP VKTPASTKRL SKTGLSKVDV REDPSILEKK
2110 2120 2130 2140 2150
TKSPGTPAPV QEENDCTAFM ETPKQKLDFT GNSSGHKRRP RTPKIRAQPL
2160 2170 2180 2190 2200
EDLDGFQELF QTPAGASDSV TVEESAKMSL ESSQAKPVKT PASTKRLSKT
2210 2220 2230 2240 2250
GLSKVDVRED PSTLGKKTKS PGRAPGTPAP VQEENDSTAF METPKQKLDF
2260 2270 2280 2290 2300
AENSSGSKRR SRTSKNRSQP LEDLDGFQEL FQTPAGASNP VSVEESAKIS
2310 2320 2330 2340 2350
LESSQAEPVR TRASTKRLSK TGLNKMDVRE GHSPLSKSSC ASQKVMQTLT
2360 2370 2380 2390 2400
LGEDHGRETK DGKVLLAQKL EPAIYVTRGK RQQRSCKKRS QSPEDLSGVQ
2410 2420 2430 2440 2450
EVFQTSGHNK DSVTVDNLAK LPSSSPPLEP TDTSVTSRRQ ARTGLRKVHV
2460 2470 2480 2490 2500
KNELSGGIMH PQISGEIVDL PREPEGEGKV IKTRKQSVKR KLDTEVNVPR
2510 2520 2530 2540 2550
SKRQRITRAE KTLEDLPGFQ ELCQAPSLVM DSVIVEKTPK MPDKSPEPVD
2560 2570 2580 2590 2600
TTSETQARRR LRRLVVTEEP IPQRKTTRVV RQTRNTQKEP ISDNQGMEEF
2610 2620 2630 2640 2650
KESSVQKQDP SVSLTGRRNQ PRTVKEKTQP LEELTSFQEE TAKRISSKSP
2660 2670 2680 2690 2700
QPEEKETLAG LKRQLRIQLI NDGVKEEPTA QRKQPSRETR NTLKEPVGDS
2710 2720 2730 2740 2750
INVEEVKKST KQKIDPVASV PVSKRPRRVP KEKAQALELA GLKGPIQTLG
2760 2770 2780 2790 2800
HTDESASDKG PTQMPCNSLQ PEQVDSFQSS PRRPRTRRGK VEADEEPSAV
2810 2820 2830 2840 2850
RKTVSTSRQT MRSRKVPEIG NNGTQVSKAS IKQTLDTVAK VTGSRRQLRT
2860 2870 2880 2890 2900
HKDGVQPLEV LGDSKEITQI SDHSEKLAHD TSILKSTQQQ KPDSVKPLRT
2910 2920 2930 2940 2950
CRRVLRASKE DPKEVLVDTR DHATLQSKSN PLLSPKRKSA RDGSIVRTRA
2960 2970 2980 2990 3000
LRSLAPKQEA TDEKPVPEKK RAASSKRHVS PEPVKMKHLK IVSNKLESVE
3010 3020 3030 3040 3050
EQVSTVMKTE EMEAKRENPV TPDQNSRYRK KTNVKQPRPK FDASAENVGI
3060 3070 3080 3090 3100
KKNEKTMKTA SQETELQNPD DGAKKSTSRG QVSGKRTCLR SRGTTEMPQP
3110 3120 3130 3140 3150
CEAEEKTSKP AAEILIKPQE EKGVSGESDV RCLRSRKTRV ALDSEPKPRV
3160 3170
TRGTKKDAKT LKEDEDIVCT KKLRTRS
Length:3,177
Mass (Da):350,864
Last modified:April 5, 2011 - v1
Checksum:i969A3BE300D755B0
GO
Isoform 2 (identifier: E9PVX6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1150-1390: Missing.

Show »
Length:2,936
Mass (Da):324,718
Checksum:iC7E2B31758362698
GO

Sequence cautioni

The sequence AAH53453 differs from that shown. Reason: Frameshift at position 2463.Curated
The sequence CAA58026 differs from that shown. Reason: Frameshift at positions 46, 54, 194, 213, 2853 and 2860.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti344E → G in CAA58026 (PubMed:8834799).Curated1
Sequence conflicti364 – 365AA → TP in CAA58026 (PubMed:8834799).Curated2
Sequence conflicti372A → G in CAA58026 (PubMed:8834799).Curated1
Sequence conflicti384 – 385KK → PQ in CAA58026 (PubMed:8834799).Curated2
Sequence conflicti401K → N in CAA58026 (PubMed:8834799).Curated1
Sequence conflicti546K → N in CAA58026 (PubMed:8834799).Curated1
Sequence conflicti622Q → P in CAA58026 (PubMed:8834799).Curated1
Sequence conflicti1079E → G in CAA58026 (PubMed:8834799).Curated1
Sequence conflicti2911D → V in CAA58026 (PubMed:8834799).Curated1
Sequence conflicti2978H → Y in CAA58026 (PubMed:8834799).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0585511150 – 1390Missing in isoform 2. Add BLAST241

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82786 mRNA. Translation: CAA58026.1. Frameshift.
AC123047 Genomic DNA. No translation available.
BC053453 mRNA. Translation: AAH53453.1. Frameshift.
CCDSiCCDS52421.1. [E9PVX6-1]
PIRiT30249.
RefSeqiNP_001074586.2. NM_001081117.2. [E9PVX6-1]
UniGeneiMm.4078.

Genome annotation databases

EnsembliENSMUST00000033310; ENSMUSP00000033310; ENSMUSG00000031004. [E9PVX6-1]
GeneIDi17345.
KEGGimmu:17345.
UCSCiuc009kem.2. mouse. [E9PVX6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82786 mRNA. Translation: CAA58026.1. Frameshift.
AC123047 Genomic DNA. No translation available.
BC053453 mRNA. Translation: AAH53453.1. Frameshift.
CCDSiCCDS52421.1. [E9PVX6-1]
PIRiT30249.
RefSeqiNP_001074586.2. NM_001081117.2. [E9PVX6-1]
UniGeneiMm.4078.

3D structure databases

ProteinModelPortaliE9PVX6.
SMRiE9PVX6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4109797.
STRINGi10090.ENSMUSP00000033310.

PTM databases

iPTMnetiE9PVX6.
PhosphoSitePlusiE9PVX6.

Proteomic databases

MaxQBiE9PVX6.
PaxDbiE9PVX6.
PeptideAtlasiE9PVX6.
PRIDEiE9PVX6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033310; ENSMUSP00000033310; ENSMUSG00000031004. [E9PVX6-1]
GeneIDi17345.
KEGGimmu:17345.
UCSCiuc009kem.2. mouse. [E9PVX6-1]

Organism-specific databases

CTDi4288.
MGIiMGI:106035. Mki67.

Phylogenomic databases

eggNOGiENOG410JR7C. Eukaryota.
ENOG410ZXYW. LUCA.
GeneTreeiENSGT00530000064425.
HOGENOMiHOG000113223.
HOVERGENiHBG006213.
InParanoidiE9PVX6.
KOiK17582.
OMAiTGLSKVD.
OrthoDBiEOG091G005S.
TreeFamiTF336000.

Miscellaneous databases

ChiTaRSiMki67. mouse.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031004.
GenevisibleiE9PVX6. MM.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR012568. K167R.
IPR029334. PP1-bd.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
PF08065. K167R. 17 hits.
PF15276. PP1_bind. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
SM01295. K167R. 16 hits.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKI67_MOUSE
AccessioniPrimary (citable) accession number: E9PVX6
Secondary accession number(s): Q61769, Q7TSF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2016
Last sequence update: April 5, 2011
Last modified: November 30, 2016
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was thought to play a key role in cell proliferation, and is commonly used as a marker of cell proliferation. However, its primary function is uncoupled from cell proliferation (PubMed:26949251). Required to maintain mitotic chromosomes dispersed by forming a steric and electrostatic charge barrier (PubMed:27362226).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.