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Protein

Serine/threonine-protein kinase 10

Gene

Stk10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in regulation of lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Inhibited by the pyrrole-indolinone inhibitor SU11274 (K00593): intercalates between the ATP-binding Lys-65 and alpha-C glutamate (Glu-81), resulting in a partial disordering of the lysine side chain. Also specifically inhibited by erlotinib. Slightly inhibited by gefitinib (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei65 – 651ATPPROSITE-ProRule annotation
Binding sitei111 – 1111InhibitorBy similarity
Binding sitei113 – 1131InhibitorBy similarity
Binding sitei117 – 1171Inhibitor; via amide nitrogenBy similarity
Active sitei157 – 1571Proton acceptorPROSITE-ProRule annotation
Binding sitei175 – 1751InhibitorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 509ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 10 (EC:2.7.11.1)
Gene namesi
Name:Stk10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3779. Stk10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967Serine/threonine-protein kinase 10PRO_0000414711Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei20 – 201PhosphoserineCombined sources
Modified residuei185 – 1851Phosphothreonine; by autocatalysisBy similarity
Modified residuei191 – 1911Phosphoserine; by autocatalysisBy similarity
Modified residuei438 – 4381PhosphoserineBy similarity
Modified residuei450 – 4501PhosphoserineBy similarity
Modified residuei454 – 4541PhosphoserineBy similarity
Modified residuei485 – 4851PhosphoserineBy similarity
Modified residuei514 – 5141PhosphoserineCombined sources
Modified residuei549 – 5491PhosphoserineBy similarity
Modified residuei951 – 9511PhosphothreonineBy similarity

Post-translational modificationi

Autophosphorylates following homodimerization, leading to activation of the protein.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiE9PTG8.
PeptideAtlasiE9PTG8.
PRIDEiE9PTG8.

PTM databases

iPTMnetiE9PTG8.

Interactioni

Subunit structurei

Homodimer; homodimerization is required for activation segment autophosphorylation.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044325.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 294259Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni175 – 22450Activation segmentBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi750 – 884135Gln-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0579. Eukaryota.
ENOG410XPQN. LUCA.
InParanoidiE9PTG8.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR022165. PKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF12474. PKK. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E9PTG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFANFRRIL RLSTFEKRKS REYEHVRRDL DPNDVWEILG ELGDGAFGKV
60 70 80 90 100
YKAKNKETGA LAAAKVIETK SEEELEDYIV EIEILATCDH PYIVKLLGAY
110 120 130 140 150
YYDGKLWIMI EFCPGGAVDA IMLELDRGLT EPQIQVVCRQ MLEALNFLHG
160 170 180 190 200
KRIIHRDLKA GNVLMTLEGD IRLADFGVSA KNLKTLQKRD SFIGTPYWMA
210 220 230 240 250
PEVVLCETMK DAPYDYKADI WSLGITLIEM AQIEPPHHEL NPMRVLLKIA
260 270 280 290 300
KSDPPTLLTP SKWSTEFRDF LKIALDKNPE TRPSAAQLLQ HPFVSTVTSN
310 320 330 340 350
KALRELVAEA KAEVMEEIED GKEDGSEKDA VSAVPPPVNH TQDSSANGTQ
360 370 380 390 400
PSLNSDKLLQ DSSTPLPPSQ PQEPVNGPCN QPSGDGSPQN TSPADEVSKN
410 420 430 440 450
DNGLKVPVPL RKSRPLSVDA RIQVTEEKQI TDQAENPSSA ASKPPKVNQS
460 470 480 490 500
RPNSSALETL GVETLANGGL ELPGSVTPNH SKRASDCSNL STSESMDYGT
510 520 530 540 550
SLSADLSLNK ETGSLSLKGS KLHNKTLKRT RRFVVDGVEV SITTSKIISE
560 570 580 590 600
DEKKDEEMRF LRRQELRELR LLQKEEHRNQ TQLSTKHELQ LEQMHRRFEQ
610 620 630 640 650
EINAKKKFYD VELENLERQQ KQQVEKMEQD HSVRRREEAK RIRLEQDRDY
660 670 680 690 700
ARFQEQLKQM KKEVKRSIEK ALRKQRQESM RMGQDSHTQK KQRLDRDFVA
710 720 730 740 750
KQKEDLELAM KKLTAENRRE ICDKERDCLN KKQELLRDRE AALWEMEEHQ
760 770 780 790 800
LQERHQLVKQ QLKDQYFLQR HDLLRKHEKE REQMQRYNQR MMEQLKVRQQ
810 820 830 840 850
QEKARLPKIQ RSDGKTRMAM YKKSLHINGA GSASEQREKV KQFSQQEEKR
860 870 880 890 900
QKAERLQQQQ KHENQMRDMV AQCESNMNEL QQLQNEKCHL LVEHETQKLK
910 920 930 940 950
ALDESHNQSL KEWRDKLRPR KKALEEDLNQ KKREQEMFFR LSEEAETRPT
960
TPNRASKFFP YSSGDAS
Length:967
Mass (Da):111,877
Last modified:April 5, 2011 - v1
Checksum:i5E48067D31F5DF8E
GO

Sequence databases

UniGeneiRn.4190.

Genome annotation databases

UCSCiRGD:3779. rat.

Cross-referencesi

Sequence databases

UniGeneiRn.4190.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044325.

PTM databases

iPTMnetiE9PTG8.

Proteomic databases

PaxDbiE9PTG8.
PeptideAtlasiE9PTG8.
PRIDEiE9PTG8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:3779. rat.

Organism-specific databases

RGDi3779. Stk10.

Phylogenomic databases

eggNOGiKOG0579. Eukaryota.
ENOG410XPQN. LUCA.
InParanoidiE9PTG8.

Miscellaneous databases

PROiE9PTG8.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR022165. PKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF12474. PKK. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTK10_RAT
AccessioniPrimary (citable) accession number: E9PTG8
Secondary accession number(s): D3ZUC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: April 5, 2011
Last modified: July 6, 2016
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.