CLP1

Functionⁱ

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.UniRule annotation

Catalytic activityⁱ

ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.UniRule annotation

ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA.UniRule annotation

Cofactorⁱ

Mg²⁺UniRule annotation, Mn²⁺UniRule annotation, Ni²⁺UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	33	ATPUniRule annotation		1
Binding siteⁱ	73	ATP; via carbonyl oxygenUniRule annotation		1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-HAMAP
ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Source: UniProtKB-EC
ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity Source: UniProtKB-HAMAP

Complete GO annotation...

GO - Biological processⁱ

mRNA 3'-end processing Source: UniProtKB-HAMAP
siRNA loading onto RISC involved in RNA interference Source: UniProtKB-HAMAP
tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

Complete GO annotation...

Keywordsⁱ

Molecular function	KinaseUniRule annotation, Transferase
Biological process	mRNA processingUniRule annotationSAAS annotation, tRNA processingUniRule annotation
Ligand	ATP-bindingSAAS annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, NickelUniRule annotation, Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Polyribonucleotide 5'-hydroxyl-kinase Clp1UniRule annotation (EC:2.7.1.78UniRule annotation) Alternative name(s): Polyadenylation factor Clp1UniRule annotation Polynucleotide kinase Clp1UniRule annotation Pre-mRNA cleavage complex II protein Clp1UniRule annotation
Gene namesⁱ	Name:CLP1UniRule annotationImported
Organismⁱ	Homo sapiens (Human)Imported
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 11

Organism-specific databases

HGNCⁱ

HGNC:16999. CLP1.

Subcellular locationⁱ

Nucleus
UniRule annotation
Automatic assertion according to rulesⁱ
- HAMAP-Rule:MF_03035
SAAS annotation
Automatic assertion according to rulesⁱ
- SAAS:SAAS00533943

GO - Cellular componentⁱ

cytosol Source: HPA
mRNA cleavage factor complex Source: UniProtKB-HAMAP
nucleoplasm Source: HPA
tRNA-intron endonuclease complex Source: UniProtKB-HAMAP

Complete GO annotation...

Keywords - Cellular componentⁱ

NucleusUniRule annotationSAAS annotation

Pathology & Biotechⁱ

Organism-specific databases

Open Targets More...OpenTargetsⁱ	ENSG00000172409.

OpenTargetsⁱ

ENSG00000172409.

PTM / Processingⁱ

Proteomic databases

EPDⁱ	E9PL17.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	E9PL17.
PaxDbⁱ	E9PL17.
PRIDEⁱ	E9PL17.

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000172409.
ExpressionAtlasⁱ	E9PL17. baseline and differential.

Interactionⁱ

Subunit structureⁱ

Component of the tRNA splicing endonuclease complex, composed of CLP1, TSEN2, TSEN15, TSEN34 and TSEN54. Component of pre-mRNA cleavage complex II (CF-II). Also associates with numerous components of the pre-mRNA cleavage complex I (CF-I/CFIm), including NUDT21, CPSF2, CPSF3, CPSF6 and CPSF7. Interacts with CSTF2 and SYMPK.UniRule annotation

Protein-protein interaction databases

IntActⁱ	E9PL17. 7 interactors.

IntActⁱ

E9PL17. 7 interactors.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	E9PL17.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	27 – 118	CLP1_NInterPro annotationAdd BLAST	92
Domainⁱ	132 – 318	CLP1_PInterPro annotationAdd BLAST	187
Domainⁱ	324 – 434	Clp1InterPro annotationAdd BLAST	111

Sequence similaritiesⁱ

Belongs to the Clp1 family. Clp1 subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGⁱ	KOG2749. Eukaryota. COG5623. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000000344.
OMAⁱ	KVAIFTY.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G088L.
PhylomeDBⁱ	E9PL17.

Family and domain databases

HAMAPⁱ	MF_03035. Clp1. 1 hit.
InterProⁱ	View protein in InterPro IPR028606. Clp1. IPR032324. Clp1_N. IPR032319. CLP1_P. IPR027417. P-loop_NTPase. IPR010655. Pre-mRNA_cleavage_Clp1.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06807. Clp1. 1 hit. PF16573. CLP1_N. 1 hit. PF16575. CLP1_P. 1 hit.
SUPFAMⁱ	SSF52540. SSF52540. 3 hits.

Sequenceⁱ

Sequence statusⁱ: Complete.

E9PL17-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTDLHSSYTE EMGEEANDDK KPTTKFELER ETELRFEVEA SQSVQLELLT 
        60         70         80         90        100
GMAEIFGTEL TRNKKFTFDA GAKVAVFTWH GCSVQLSGRT EVAYVSKDTP 
       110        120        130        140        150
MLLYLNTHTA LEQMRRQAEK EEERGPRVMV VGPTDVGKST VCRLLLNYAV 
       160        170        180        190        200
RLGRRPTYVE LDVGQGSVSI PGTMGALYIE RPADVEEGFS IQAPLVYHFG 
       210        220        230        240        250
STTPGTNIKL YNKITSRLAD VFNQRCEVNR RASVSGCVIN TCGWVKGSGY 
       260        270        280        290        300
QALVHAASAF EVDVVVVLDQ ERLYNELKRD LPHFVRTVLL PKSGGVVERS 
       310        320        330        340        350
KDFRRECRDE RIREYFYGFR GCFYPHAFNV KFSDVKIYKV GAPTIPDSCL 
       360        370        380        390        400
PLGMSQEDNQ LKLVPVTPGR DMVHHLLSVS TAEGTEENLS ETSVAGFIVV 
       410        420        430 
TSVDLEHQVF TVLSPAPRPL PKNFLLIMDI RFMDLK

Length:436

Mass (Da):48,940

Last modified:April 5, 2011 - v1

Checksum:ⁱ51576E4951199285

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AP000662 Genomic DNA. No translation available. KF511152 Genomic DNA. No translation available.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AP000662 Genomic DNA. No translation available.
KF511152 Genomic DNA. No translation available.

Genome annotation databases

Ensemblⁱ	ENST00000529430; ENSP00000433406; ENSG00000172409.
UCSC genome browser More...UCSCⁱ	uc058bns.1. human.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AP000662 Genomic DNA. No translation available. KF511152 Genomic DNA. No translation available.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AP000662 Genomic DNA. No translation available.
KF511152 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortalⁱ	E9PL17.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	E9PL17. 7 interactors.

IntActⁱ

E9PL17. 7 interactors.

Proteomic databases

EPDⁱ	E9PL17.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	E9PL17.
PaxDbⁱ	E9PL17.
PRIDEⁱ	E9PL17.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000529430; ENSP00000433406; ENSG00000172409.
UCSC genome browser More...UCSCⁱ	uc058bns.1. human.

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:16999. CLP1.
Open Targets More...OpenTargetsⁱ	ENSG00000172409.
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG2749. Eukaryota. COG5623. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000000344.
OMAⁱ	KVAIFTY.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G088L.
PhylomeDBⁱ	E9PL17.

Gene expression databases

Bgeeⁱ	ENSG00000172409.
ExpressionAtlasⁱ	E9PL17. baseline and differential.

Family and domain databases

HAMAPⁱ	MF_03035. Clp1. 1 hit.
InterProⁱ	View protein in InterPro IPR028606. Clp1. IPR032324. Clp1_N. IPR032319. CLP1_P. IPR027417. P-loop_NTPase. IPR010655. Pre-mRNA_cleavage_Clp1.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06807. Clp1. 1 hit. PF16573. CLP1_N. 1 hit. PF16575. CLP1_P. 1 hit.
SUPFAMⁱ	SSF52540. SSF52540. 3 hits.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	E9PL17_HUMAN
Accessionⁱ	E9PL17Primary (citable) accession number: E9PL17
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	April 5, 2011
	Last sequence update:	April 5, 2011
	Last modified:	July 5, 2017
	This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.Imported

Keywords - Technical termⁱ

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - E9PL17 (E9PL17_HUMAN)

Your basket is currently empty.

Polyribonucleotide 5'-hydroxyl-kinase Clp1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Caution

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ