ID   E9PG37_HUMAN            Unreviewed;       532 AA.
AC   E9PG37;
DT   05-APR-2011, integrated into UniProtKB/TrEMBL.
DT   05-APR-2011, sequence version 1.
DT   27-MAR-2024, entry version 87.
DE   RecName: Full=Rho guanine nucleotide exchange factor 3 {ECO:0000256|ARBA:ARBA00023847};
GN   Name=ARHGEF3 {ECO:0000313|Ensembl:ENSP00000420420.1};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606 {ECO:0000313|Ensembl:ENSP00000420420.1, ECO:0000313|Proteomes:UP000005640};
RN   [1] {ECO:0000313|Ensembl:ENSP00000420420.1, ECO:0000313|Proteomes:UP000005640}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16641997; DOI=10.1038/nature04728;
RA   Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA   Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA   Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA   Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA   Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA   Sodergren E., Song X.Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA   Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA   Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA   Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA   Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA   Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA   Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA   Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.V., Okwuonu G.O.,
RA   Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA   Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA   Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA   Wang J., Wang Q., Williams G.A., Wong G.K., Yao Z., Zhang J., Zhang X.,
RA   Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA   Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT   "The DNA sequence, annotation and analysis of human chromosome 3.";
RL   Nature 440:1194-1198(2006).
RN   [2] {ECO:0007829|PubMed:23186163}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=23186163;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
RN   [3] {ECO:0000313|Ensembl:ENSP00000420420.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- FUNCTION: Acts as a guanine nucleotide exchange factor (GEF) for RhoA
CC       and RhoB GTPases. {ECO:0000256|ARBA:ARBA00043905}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR   EMBL; AC097358; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC099327; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC099559; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC099781; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_001276627.1; NM_001289698.1.
DR   AlphaFoldDB; E9PG37; -.
DR   SMR; E9PG37; -.
DR   MassIVE; E9PG37; -.
DR   MaxQB; E9PG37; -.
DR   PeptideAtlas; E9PG37; -.
DR   ProteomicsDB; 20237; -.
DR   Antibodypedia; 31503; 207 antibodies from 24 providers.
DR   DNASU; 50650; -.
DR   Ensembl; ENST00000496106.5; ENSP00000420420.1; ENSG00000163947.12.
DR   GeneID; 50650; -.
DR   UCSC; uc011bew.3; human.
DR   CTD; 50650; -.
DR   HGNC; HGNC:683; ARHGEF3.
DR   VEuPathDB; HostDB:ENSG00000163947; -.
DR   GeneTree; ENSGT00940000158385; -.
DR   OrthoDB; 24012at2759; -.
DR   BioGRID-ORCS; 50650; 18 hits in 1153 CRISPR screens.
DR   ChiTaRS; ARHGEF3; human.
DR   GenomeRNAi; 50650; -.
DR   Proteomes; UP000005640; Chromosome 3.
DR   Bgee; ENSG00000163947; Expressed in renal glomerulus and 194 other cell types or tissues.
DR   ExpressionAtlas; E9PG37; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR   GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR   CDD; cd10572; PH_RhoGEF3_XPLN; 1.
DR   CDD; cd00160; RhoGEF; 1.
DR   Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR   Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR   InterPro; IPR035899; DBL_dom_sf.
DR   InterPro; IPR000219; DH-domain.
DR   InterPro; IPR001331; GDS_CDC24_CS.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR001849; PH_domain.
DR   InterPro; IPR044129; PH_RhoGEF3_XPLN.
DR   PANTHER; PTHR46006:SF2; RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 3; 1.
DR   PANTHER; PTHR46006; RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A; 1.
DR   Pfam; PF00621; RhoGEF; 1.
DR   SMART; SM00233; PH; 1.
DR   SMART; SM00325; RhoGEF; 1.
DR   SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR   SUPFAM; SSF50729; PH domain-like; 1.
DR   PROSITE; PS00741; DH_1; 1.
DR   PROSITE; PS50010; DH_2; 1.
DR   PROSITE; PS50003; PH_DOMAIN; 1.
PE   1: Evidence at protein level;
KW   Proteomics identification {ECO:0007829|EPD:E9PG37,
KW   ECO:0007829|MaxQB:E9PG37};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005640}.
FT   DOMAIN          128..310
FT                   /note="DH"
FT                   /evidence="ECO:0000259|PROSITE:PS50010"
FT   DOMAIN          297..455
FT                   /note="PH"
FT                   /evidence="ECO:0000259|PROSITE:PS50003"
FT   REGION          470..532
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        470..485
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        515..532
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   532 AA;  60790 MW;  A8A32FEA5303B6D7 CRC64;
     MRSERPMVWC CLFVRSQRKR KQSTQDEDAV SLCSLDISEP SNKRVKPLSR VTSLANLIPP
     VKATPLKRFS QTLQRSISFR SESRPDILAP RPWSRNAAPS STKRRDSKLW SETFDVCVNQ
     MLTSKEIKRQ EAIFELSQGE EDLIEDLKLA KKAYHDPMLK LSIMTEQELN QIFGTLDSLI
     PLHEELLSQL RDVRKPDGST EHVGPILVGW LPCLSSYDSY CSNQVAAKAL LDHKKQDHRV
     QDFLQRCLES PFSRKLDLWN FLDIPRSRLV KYPLLLREIL RHTPNDNPDQ QHLEEAINII
     QGIVAEINTK TGESECRYYK ERLLYLEEGQ KDSLIDSSRV LCCHGELKNN RGVKLHVFLF
     QEVLVITRAV THNEQLCYQL YRQPIPVKDL LLEDLQDGEV RLGGSLRGAF SNNERIKNFF
     RVSFKNGSQS QTHSLQANDT FNKQQWLNCI RQAKETVLCA AGQAGVLDSE GSFLNPTTGS
     RELQGETKLE QMDQSDSESD CSMDTSEVSL DCERMEQTDS SCGNSRHGES NV
//