ID   A8CTZ0_HUMAN            Unreviewed;       877 AA.
AC   A8CTZ0; E9PD98;
DT   13-NOV-2007, integrated into UniProtKB/TrEMBL.
DT   13-NOV-2007, sequence version 1.
DT   27-MAR-2024, entry version 114.
DE   SubName: Full=Intersectin 1 {ECO:0000313|EMBL:ABV24869.1, ECO:0000313|Ensembl:ENSP00000370685.5};
GN   Name=ITSN1 {ECO:0000313|EMBL:ABV24869.1,
GN   ECO:0000313|Ensembl:ENSP00000370685.5};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606 {ECO:0000313|EMBL:ABV24869.1};
RN   [1] {ECO:0000313|Ensembl:ENSP00000370685.5, ECO:0000313|Proteomes:UP000005640}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10830953; DOI=10.1038/35012518;
RG   Chromosome 21 mapping and sequencing consortium;
RA   Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S.,
RA   Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M.,
RA   Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A., Menzel U.,
RA   Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A.,
RA   Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J.,
RA   Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K.,
RA   Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G.,
RA   Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J.,
RA   Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S.,
RA   Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K.,
RA   Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.;
RT   "The DNA sequence of human chromosome 21.";
RL   Nature 405:311-319(2000).
RN   [2] {ECO:0007829|PubMed:18669648}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA   Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA   Elledge S.J., Gygi S.P.;
RT   "A quantitative atlas of mitotic phosphorylation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN   [3] {ECO:0007829|PubMed:19690332}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA   Mayya V., Lundgren D.H., Hwang S.I., Rezaul K., Wu L., Eng J.K.,
RA   Rodionov V., Han D.K.;
RT   "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT   reveals system-wide modulation of protein-protein interactions.";
RL   Sci. Signal. 2:RA46-RA46(2009).
RN   [4] {ECO:0000313|EMBL:ABV24869.1}
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Lung {ECO:0000313|EMBL:ABV24869.1};
RX   PubMed=19777371; DOI=10.1007/s11033-009-9824-8;
RA   Kropyvko S., Gerasymchuk D., Skrypkina I., Dergai M., Dergai O.,
RA   Nikolaienko O., Rynditch A., Tsyba L.;
RT   "Structural diversity and differential expression of novel human
RT   intersectin 1 isoforms.";
RL   Mol. Biol. Rep. 37:2789-2796(2010).
RN   [5] {ECO:0007829|PubMed:20068231}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA   Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA   Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT   "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT   site occupancy during mitosis.";
RL   Sci. Signal. 3:RA3-RA3(2010).
RN   [6] {ECO:0007829|PubMed:21269460}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Burckstummer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [7] {ECO:0007829|PubMed:23186163}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=23186163;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
RN   [8] {ECO:0007829|PubMed:24275569}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
RN   [9] {ECO:0000313|Ensembl:ENSP00000370685.5}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
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DR   EMBL; AP000308; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP000309; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP000310; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP000311; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP000312; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP000313; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; KF511399; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; KF511404; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; EU120736; ABV24869.1; -; mRNA.
DR   ProteomicsDB; 19614; -.
DR   Antibodypedia; 7532; 106 antibodies from 19 providers.
DR   Ensembl; ENST00000381285.8; ENSP00000370685.5; ENSG00000205726.15.
DR   UCSC; uc061ztd.1; human.
DR   HGNC; HGNC:6183; ITSN1.
DR   VEuPathDB; HostDB:ENSG00000205726; -.
DR   GeneTree; ENSGT00940000157065; -.
DR   ChiTaRS; ITSN1; human.
DR   Proteomes; UP000005640; Chromosome 21.
DR   Bgee; ENSG00000205726; Expressed in sural nerve and 210 other cell types or tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   CDD; cd00052; EH; 2.
DR   CDD; cd11987; SH3_Intersectin1_1; 1.
DR   Gene3D; 1.10.238.10; EF-hand; 2.
DR   Gene3D; 2.30.30.40; SH3 Domains; 1.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   InterPro; IPR000261; EH_dom.
DR   InterPro; IPR032140; INTAP.
DR   InterPro; IPR036028; SH3-like_dom_sf.
DR   InterPro; IPR001452; SH3_domain.
DR   PANTHER; PTHR11216; EH DOMAIN; 1.
DR   PANTHER; PTHR11216:SF68; INTERSECTIN-1; 1.
DR   Pfam; PF12763; EF-hand_4; 2.
DR   Pfam; PF16617; INTAP; 1.
DR   Pfam; PF14604; SH3_9; 1.
DR   SMART; SM00054; EFh; 2.
DR   SMART; SM00027; EH; 2.
DR   SMART; SM00326; SH3; 1.
DR   SUPFAM; SSF47473; EF-hand; 2.
DR   SUPFAM; SSF50044; SH3-domain; 1.
DR   PROSITE; PS00018; EF_HAND_1; 2.
DR   PROSITE; PS50222; EF_HAND_2; 2.
DR   PROSITE; PS50031; EH; 2.
DR   PROSITE; PS50002; SH3; 1.
PE   1: Evidence at protein level;
KW   Calcium {ECO:0000256|ARBA:ARBA00022837};
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Proteomics identification {ECO:0007829|EPD:A8CTZ0,
KW   ECO:0007829|MaxQB:A8CTZ0};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005640};
KW   SH3 domain {ECO:0000256|ARBA:ARBA00022443, ECO:0000256|PROSITE-
KW   ProRule:PRU00192}.
FT   DOMAIN          21..100
FT                   /note="EH"
FT                   /evidence="ECO:0000259|PROSITE:PS50031"
FT   DOMAIN          53..88
FT                   /note="EF-hand"
FT                   /evidence="ECO:0000259|PROSITE:PS50222"
FT   DOMAIN          184..273
FT                   /note="EH"
FT                   /evidence="ECO:0000259|PROSITE:PS50031"
FT   DOMAIN          217..252
FT                   /note="EF-hand"
FT                   /evidence="ECO:0000259|PROSITE:PS50222"
FT   DOMAIN          703..764
FT                   /note="SH3"
FT                   /evidence="ECO:0000259|PROSITE:PS50002"
FT   REGION          285..311
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          613..664
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          795..826
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          318..472
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   COMPBIAS        293..311
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   877 AA;  100705 MW;  763906D35F88716D CRC64;
     MAQFPTPFGG SLDIWAITVE ERAKHDQQFH SLKPISGFIT GDQARNFFFQ SGLPQPVLAQ
     IWALADMNND GRMDQVEFSI AMKLIKLKLQ GYQLPSALPP VMKQQPVAIS SAPAFAAVPP
     LANGAPPVIQ PLPAFAHPAA TLPKSSSFSR SGPGSQLNTK LQKAQSFDVA SVPPVAEWAV
     PQSSRLKYRQ LFNSHDKTMS GHLTGPQART ILMQSSLPQA QLASIWNLSD IDQDGKLTAE
     EFILAMHLID VAMSGQPLPP VLPPEYIPPS FRRVRSGSGI SVISSTSVDQ RLPEEPVLED
     EQQQLEKKLP VTFEDKKREN FERGNLELEK RRQALLEQQR KEQERLAQLE RAEQERKERE
     RQEQERKRQL ELEKQLEKQR ELERQREEER RKEIERREAA KRELERQRQL EWERNRRQEL
     LNQRNKEQED IVVLKAKKKT LEFELEALND KKHQLEGKLQ DIRCRLTTQR QEIESTNKSR
     ELRIAEITHL QQQLQESQQM LGRLIPEKQI LNDQLKQVQQ NSLHRDSLVT LKRALEAKEL
     ARQHLRDQLD EVEKETRSKL QEIDIFNNQL KELREIHNKQ QLQKQKSMEA ERLKQKEQER
     KIIELEKQKE EAQRRAQERD KQWLEHVQQE DEHQRPRKLH EEEKLKREES VKKKDGEEKG
     KQEAQDKLGR LFHQHQEPAK PAVQAPWSTA EKGPLTISAQ ENVKVVYYRA LYPFESRSHD
     EITIQPGDIV MVDESQTGEP GWLGGELKGK TGWFPANYAE KIPENEVPAP VKPVTDSTSA
     PAPKLALRET PAPLAVTSSE PSTTPNNWAD FSSTWPTSTN EKPETDNWDA WAAQPSLTVP
     SAGQLRQRSA FTPATATGSS PSPVLGQHGF WFFRESC
//