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Protein

Probable dipeptidyl-aminopeptidase B

Gene

DAPB

Organism
Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) (Metarhizium anisopliae (strain ARSEF 23))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Type IV dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.By similarity

Catalytic activityi

Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei760 – 7601Charge relay systemBy similarity
Active sitei837 – 8371Charge relay systemBy similarity
Active sitei870 – 8701Charge relay systemBy similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. serine-type peptidase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dipeptidyl-aminopeptidase B (EC:3.4.14.5)
Short name:
DPAP B
Gene namesi
Name:DAPB
ORF Names:MAA_03364
OrganismiMetarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) (Metarhizium anisopliae (strain ARSEF 23))
Taxonomic identifieri655844 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesClavicipitaceaemitosporic ClavicipitaceaeMetarhizium
ProteomesiUP000002498: Unassembled WGS sequence

Subcellular locationi

Vacuole membrane By similarity; Single-pass type II membrane protein By similarity
Note: Lysosome-like vacuoles.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9494CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei95 – 11521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini116 – 915800VacuolarSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 915915Probable dipeptidyl-aminopeptidase BPRO_0000412149Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi355 – 3551N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi577 – 5771N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi819 – 8191N-linked (GlcNAc...)PROSITE-ProRule annotation

Keywords - PTMi

Glycoprotein

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9B family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK01282.
OrthoDBiEOG72VHFG.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

E9ETL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPFTDDPE SQRPSTSRLS QDSLSSVSTT SLVFDRIQEE MDRDPSASRS
60 70 80 90 100
ARRDLLPATK DEGDFDENAS ETGAFLGPPG VPLQRQPMDR GFRRILIIIG
110 120 130 140 150
AVFVGAWLAG LGIFVLSGSY KHESDSEHDP DANSRGSGKA VTMDQVFGGF
160 170 180 190 200
WSARSHSISW IADPDGGDGL LLEVNTANGY LVVEDVRADK ESTDTNAGQT
210 220 230 240 250
ADTQPAKPRV LMRDPYFMYH GEIKRPDWNE PSPDLKKVLL AVKREKNWRH
260 270 280 290 300
SFQATYFILD VETAQVQPLV PDNVNAKVQL ANWSPTSDAI SFTMDNNIYI
310 320 330 340 350
RRLNQANDVV QITKDGGPEY FYGIPDWVYE EEVFAGRSAT WWSDDGKYLA
360 370 380 390 400
FLRTNETAVP EYAIEYYIQR PSGKKPAVGE EAYPEIRKIK YPKPGAHNPV
410 420 430 440 450
VDVQYYDVSK GDVFSISAPD EFPDHDRIIS NVLWAGNKVL LKQSNRIGDF
460 470 480 490 500
LKVILVDPSQ REAKIVNSIN IAEIDGGWFE ISHTMTYIPA DPSKGRQQDG
510 520 530 540 550
YVDTVIHEGY EHIGYFTPID NPKPIMLTSG SWEVEDAPSA VDLNNNLVYF
560 570 580 590 600
VATKESSIQR HVYSVKLDGT NLTPLTNTSS EGYYTVSFSS RSGFALLSYK
610 620 630 640 650
GPKIPYQKVI STPSIPIQFS RTIEDNADLA DKAKKHELPI LKYGTLQLPN
660 670 680 690 700
GISVNYLERR PPHFNPKKKY PILFQQYSGP KSQTVTKKFA VDFQSYVASS
710 720 730 740 750
LGYLVVTIDP RGTGFLGRQH RVVVRSQLGV LEAQDHIAAA KHYSSLPYVD
760 770 780 790 800
PSRLAIWGWS YGGFQTLKTL EVDAGDTFSY GMAVAPVTDW RFYDSIYTER
810 820 830 840 850
YMRLPQDNTA GYDASAVRNA TALGMNKRFL IMHGSADDNV HFQNSLKLLD
860 870 880 890 900
YLDLAGIENY DVHVFPDSDH SIAFHGANRM VYDRLNNWLV NAFNGEWLKI
910
ADPKPIDTKK RRHVS
Length:915
Mass (Da):102,613
Last modified:March 4, 2015 - v2
Checksum:i8F1FCAEF3680F64D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ADNJ02000003 Genomic DNA. Translation: EFZ00768.2.
RefSeqiXP_007819553.1. XM_007821362.1.

Genome annotation databases

GeneIDi19257650.
KEGGimaj:MAA_03364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ADNJ02000003 Genomic DNA. Translation: EFZ00768.2.
RefSeqiXP_007819553.1. XM_007821362.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi19257650.
KEGGimaj:MAA_03364.

Phylogenomic databases

KOiK01282.
OrthoDBiEOG72VHFG.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M. acridum."
    Gao Q., Jin K., Ying S.-H., Zhang Y., Xiao G., Shang Y., Duan Z., Hu X., Xie X.-Q., Zhou G., Peng G., Luo Z., Huang W., Wang B., Fang W., Wang S., Zhong Y., Ma L.-J.
    , St Leger R.J., Zhao G.-P., Pei Y., Feng M.-G., Xia Y., Wang C.
    PLoS Genet. 7:E1001264-E1001264(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ARSEF 23 / ATCC MYA-3075.
  2. "Trajectory and genomic determinants of fungal-pathogen speciation and host adaptation."
    Hu X., Xiao G., Zheng P., Shang Y., Su Y., Zhang X., Liu X., Zhan S., St Leger R.J., Wang C.
    Proc. Natl. Acad. Sci. U.S.A. 111:16796-16801(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: ARSEF 23 / ATCC MYA-3075.

Entry informationi

Entry nameiDAPB_METRA
AccessioniPrimary (citable) accession number: E9ETL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: March 4, 2015
Last modified: March 4, 2015
This is version 19 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.