E9ED72 (DAPB_METAQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable dipeptidyl-aminopeptidase B
Short name=DPAP B
EC=3.4.14.5

Gene names

Name:	DAPB
ORF Names:	MAC_07820

Organism

Metarhizium acridum (strain CQMa 102) [Complete proteome]

Taxonomic identifier

655827 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Hypocreomycetidae › Hypocreales › Clavicipitaceae › mitosporic Clavicipitaceae › Metarhizium › Metarhizium acridum

Protein attributes

Sequence length	934 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Type IV dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline By similarity.
Catalytic activity	Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein By similarity. Note: Lysosome-like vacuoles By similarity.
Sequence similarities	Belongs to the peptidase S9B family.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Aminopeptidase Hydrolase Protease Serine protease
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 934	934	Probable dipeptidyl-aminopeptidase B		PRO_0000412148
Regions
	Topological domain	1 – 93	93	Cytoplasmic Potential
	Transmembrane	94 – 114	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	115 – 934	820	Vacuolar Potential
Sites
	Active site	759	1	Charge relay system By similarity
	Active site	836	1	Charge relay system By similarity
	Active site	869	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	354	1	N-linked (GlcNAc...) Potential
	Glycosylation	576	1	N-linked (GlcNAc...) Potential
	Glycosylation	818	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	E9ED72 [UniParc]. Last modified April 5, 2011. Version 1. Checksum: 89ED7648E4BC6754	FASTA	934	104,515
	10 20 30 40 50 60 MAPPFTDDPE SQRPSTSRLS QDSLSSVSTT SLVFDRIQEE MDRDPSSSRS ARRDLLSATK 70 80 90 100 110 120 DEGDYEDASE TGAFLGQPGV PLQRQPMDRG FRRILIIIGA VFVGAWLAGL GVFVLSGSYR 130 140 150 160 170 180 HESDSEHDPD AISRGSGKAV TMDQVFGGFW SAKSRSISWI ADPDGGDGLL LEVGTANGYL 190 200 210 220 230 240 VVEDVRADKQ GTDTTSRQTA DTQPAKPRVL MKDPYFMYNG EIKRPEWNEP SPDLKKVLLA 250 260 270 280 290 300 VNLEKNWRHS FQATYFILHV ETAQIQPLVP DNVNAKVQLA NWSPKSDAIS FTMDNNIYIR 310 320 330 340 350 360 RLNQANDVVQ ITKDGGPEYF YGIPDWVYEE EVLAGRSATW WSDDGNYLAF LRTNETAVPE 370 380 390 400 410 420 YAIEYYIQRP SGKKPAVGEE AYPEIRKIKY PKPGAHNPVV DVQYYDVSKG DVFSISAPDE 430 440 450 460 470 480 FPDKDRIISN VLWAGNKVLL KQSNRVGDFL KVILVDPSER KAKIVNSINI AEIDGGWFEI 490 500 510 520 530 540 SHTMTYIPAD PSKGRQQDGY VDTVIHEGYE HIGYFTPIDN PKPIMLTSGS WEVEDAPSAV 550 560 570 580 590 600 DLDNNLVYFV ATKESSIQRH VYSVKLDGSN LTPLTNTSSE GYYAVSFSSR SGFALLSYQG 610 620 630 640 650 660 PKIPYQKVIS TPSIPIEFSR TIEDNAGLAD KAKKHELPIL KYGTLQLPNG ISVNYLERRP 670 680 690 700 710 720 PHFNPKKKYP ILFQQYSGPK SQTVTKKFAV NFQSYVASSL GYLVVTIDPR GTGFLGRQHR 730 740 750 760 770 780 VVVRSQLGVL EAQDHIAAAK HYSSLPYVDP SRLAIWGWSY GGFQTLKTLE VDAGDTFSYG 790 800 810 820 830 840 MAVAPVTDWR FYDSIYTERY MRLPQDNAAG YDASAVHNAT ALGMSKRFLI MHGSADDNVH 850 860 870 880 890 900 FQNSLKLLDY LDLARIENYD VHVFPDSDHG IAFHGANRMV YDSKSEFSVL FSWILSPMLT 910 920 930 LSGLNNWLVN AFNGEWLKIA DPKPIDTKKR RHVS

References

[1]

"Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M. acridum." Gao Q., Jin K., Ying S.-H., Zhang Y., Xiao G., Shang Y., Duan Z., Hu X., Xie X.-Q., Zhou G., Peng G., Luo Z., Huang W., Wang B., Fang W., Wang S., Zhong Y., Ma L.-J. , St Leger R.J., Zhao G.-P., Pei Y., Feng M.-G., Xia Y., Wang C. PLoS Genet. 7:E1001264-E1001264(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CQMa 102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	GL698555 Genomic DNA. Translation: EFY86155.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001375. Peptidase_S9. IPR002469. Peptidase_S9B. [Graphical view]
Pfam	PF00930. DPPIV_N. 1 hit. PF00326. Peptidase_S9. 1 hit. [Graphical view]
PROSITE	PS00708. PRO_ENDOPEP_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPB_METAQ

Accession

Primary (citable) accession number: E9ED72

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	April 5, 2011
Last modified:	June 13, 2012
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program