E8YWD5 (E8YWD5_9BURK) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Choline dehydrogenase HAMAP-Rule MF_00750
Short name=CDH HAMAP-Rule MF_00750
Short name=CHD HAMAP-Rule MF_00750
EC=1.1.99.1 HAMAP-Rule MF_00750

Gene names

Name:	betA HAMAP-Rule MF_00750
ORF Names:	BC1001_5832 EMBL ADX59197.1

Organism

Burkholderia sp. CCGE1001 EMBL ADX59197.1

Taxonomic identifier

640510 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity. HAMAP-Rule MF_00750 SAAS SAAS000172
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP-Rule MF_00750 RuleBase RU003969 SAAS SAAS000172
Cofactor	FAD By similarity. HAMAP-Rule MF_00750 SAAS SAAS000172
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP-Rule MF_00750 RuleBase RU003969 SAAS SAAS000172
Sequence similarities	Belongs to the GMC oxidoreductase family. HAMAP-Rule MF_00750 RuleBase RU003968

Ontologies

Keywords
Ligand	FAD HAMAP-Rule MF_00750 RuleBase RU003968 SAAS SAAS011533 Flavoprotein HAMAP-Rule MF_00750 RuleBase RU003968 SAAS SAAS011533
Molecular function	Oxidoreductase HAMAP-Rule MF_00750 SAAS SAAS000172
Gene Ontology (GO)
Biological_process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP
Molecular_function	choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	7 – 36	30	FAD By similarity HAMAP-Rule MF_00750
Sites
	Active site	474	1	By similarity HAMAP-Rule MF_00750

Sequences

	Sequence		Length	Mass (Da)
	E8YWD5 [UniParc]. Last modified April 5, 2011. Version 1. Checksum: EA4EC4217322DA1E	FASTA	561	62,131
	10 20 30 40 50 60 MSSNEYDYII VGAGSAGNVL ASRLTEDADV TVLLLEAGGP DYRFDFRTQM PAALAYPLQG 70 80 90 100 110 120 RRYNWAYETD PEPHMNNRRM ECGRGKGLGG SSLINGMCYI RGNALDYDGW AERPGLENWT 130 140 150 160 170 180 YLDCLPYFRK AETRDVGPNA YHGGDGPVHV TTSKPGNNPL FAAMVEAGVQ AGFPRTDDLN 190 200 210 220 230 240 GYQQEGFGPM DRTVTANGRR ASTARGYLDR AKGRANLTIV THALSDRVLF SGKRAVGVAY 250 260 270 280 290 300 LHGGATVNAH ARREVLVCSG AIASPQLLQR SGVGRSTWLR ELDIPLVHDL PGVGENLQDH 310 320 330 340 350 360 LEMYIQYECK EPVSLYPALQ WWNQPAIGLE WMLNGTGIGA SNQFEAGGFI RTRDDDLWPN 370 380 390 400 410 420 IQYHFLPVAI NYNGSNAIKM HGFQAHVGSM RSPSRGRVKL TSRDPAAHPS ILFNYMADPL 430 440 450 460 470 480 DWREFRDGIR ITREIMRQPA LDRYRGRELN PGAELTTDAQ LDTFVRARAE TAFHPSCSCK 490 500 510 520 530 540 MGYDDMAVVD NEGRVHGMQG LRVVDASIMP RITTGNLNAP TIMLAEKIAD RIRQRAPLAR 550 560 VETPYFVAHG APSRKQERAT V

References

[1]

"Complete sequence of chromosome2 of Burkholderia sp. CCGE1001." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Chertkov O., Saunders E., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Mikhailova N., Pagani I., Martinez-Romero E. , Rogel M.A., Auchtung J., Tiedje J., Woyke T. Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: CCGE1001 EMBL ADX59197.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002520 Genomic DNA. Translation: ADX59197.1.
RefSeq	YP_004232257.1. NC_015137.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADX59197; ADX59197; BC1001_5832.
GeneID	10227440.
KEGG	bug:BC1001_5832.
PATRIC	46904914. VBIBurSp1058_6101.
Phylogenomic databases
KO	K00108.
Enzyme and pathway databases
BioCyc	BSP640510:GI28-2247-MONOMER.
UniPathway	UPA00529; UER00385.
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen.
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E8YWD5_9BURK

Accession

Primary (citable) accession number: E8YWD5

Entry history

Integrated into UniProtKB/TrEMBL:	April 5, 2011
Last sequence update:	April 5, 2011
Last modified:	April 3, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)