Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

E8Y987 (E8Y987_ECOKO) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Glutaminase HAMAP-Rule MF_00313

EC=3.5.1.2 HAMAP-Rule MF_00313
Gene names
Name:yneH EMBL AFH18078.1
Synonyms:glsA HAMAP-Rule MF_00313
Ordered Locus Names:EKO11_2292 EMBL ADX50901.1
ORF Names:KO11_15545 EMBL AFH18078.1
OrganismEscherichia coli (strain ATCC 55124 / KO11) [Complete proteome] [HAMAP] EMBL ADX50901.1
Taxonomic identifier595495 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-glutamine + H2O = L-glutamate + NH3. SAAS SAAS012338 HAMAP-Rule MF_00313

Subunit structure

Homotetramer By similarity. SAAS SAAS012338 HAMAP-Rule MF_00313

Sequence similarities

Belongs to the glutaminase family. HAMAP-Rule MF_00313

Ontologies

Keywords
   Molecular functionHydrolase SAAS SAAS012338 HAMAP-Rule MF_00313 EMBL ADX50901.1
   PTMAcetylation HAMAP-Rule MF_00313
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamine metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionglutaminase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Binding site661Substrate By similarity HAMAP-Rule MF_00313
Binding site1171Substrate By similarity HAMAP-Rule MF_00313
Binding site1611Substrate By similarity HAMAP-Rule MF_00313
Binding site1681Substrate By similarity HAMAP-Rule MF_00313
Binding site1921Substrate By similarity HAMAP-Rule MF_00313
Binding site2441Substrate By similarity HAMAP-Rule MF_00313
Binding site2621Substrate; via amide nitrogen By similarity HAMAP-Rule MF_00313

Sequences

Sequence LengthMass (Da)Tools
E8Y987 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: DDC39D9DC0D8A651

FASTA30833,516
        10         20         30         40         50         60 
MAVAMDNAIL ENILRQVRPL IGQGKVADYI PALATVDGSR LGIAICTVDG QLFQAGDAQE 

        70         80         90        100        110        120 
RFSIQSISKV LSLVVAMRHY SEEEIWQRVG KDPSGSPFNS LVQLEMEQGI PRNPFINAGA 

       130        140        150        160        170        180 
LVVCDMLQGR LSAPRQRMLE VVRGLSGVSD ISYDTVVARS EFEHSARNAA IAWLMKSFGN 

       190        200        210        220        230        240 
FHHDVTTVLQ NYFHYCALKM SCVELARTFV FLANQGKAIH IDEPVVTPMQ ARQINALMAT 

       250        260        270        280        290        300 
SGMYQNAGEF AWRVGLPAKS GVGGGIVAIV PHEMAIAVWS PELDDAGNSL AGIAVLEQLT 


KQLGRSVY 

« Hide

References

[1]"Complete sequence of chromosome of Escherichia coli KO11."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Munk A.C., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Keating D., Landick R., Woyke T.
Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 55124 / KO11 and KO11 EMBL ADX50901.1.
[2]"Optical mapping and sequencing of the Escherichia coli KO11 genome reveal extensive chromosomal rearrangements, and multiple tandem copies of the Zymomonas mobilis pdc and adhB genes."
Turner P.C., Yomano L.P., Jarboe L.R., York S.W., Baggett C.L., Moritz B.E., Zentz E.B., Shanmugam K.T., Ingram L.O.
J. Ind. Microbiol. Biotechnol. 39:629-639(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KO11FL EMBL AFH18078.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002516 Genomic DNA. Translation: ADX50901.1.
CP002970 Genomic DNA. Translation: AFH18078.1.
RefSeqYP_005277965.1. NC_016902.1.
YP_006165430.1. NC_017660.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADX50901; ADX50901; EKO11_2292.
AFH18078; AFH18078; KO11_15545.
GeneID11777112.
12763601.
KEGGekf:KO11_15545.
eko:EKO11_2292.
PATRIC48644556. VBIEscCol13896_2320.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01425.
OMAMSIAVWS.

Enzyme and pathway databases

BioCycECOL595495:GI1Q-2336-MONOMER.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
HAMAPMF_00313. Glutaminase.
InterProIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERPTHR12544. PTHR12544. 1 hit.
PfamPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMSSF56601. SSF56601. 1 hit.
TIGRFAMsTIGR03814. Gln_ase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameE8Y987_ECOKO
AccessionPrimary (citable) accession number: E8Y987
Entry history
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: July 9, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)