Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

E8Y1L0

- E8Y1L0_ECOKO

UniProt

E8Y1L0 - E8Y1L0_ECOKO

Protein

Elongation factor Tu

Gene

tufA

Organism
Escherichia coli (strain ATCC 55124 / KO11)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 31 (01 Oct 2014)
      Sequence version 1 (05 Apr 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 268GTPUniRule annotation
    Nucleotide bindingi81 – 855GTPUniRule annotation
    Nucleotide bindingi136 – 1394GTPUniRule annotation

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-HAMAP
    3. translation elongation factor activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Elongation factorUniRule annotationImported, HydrolaseImported

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-bindingUniRule annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciECOL595495:GI1Q-408-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor TuUniRule annotation
    Short name:
    EF-TuUniRule annotation
    Gene namesi
    Name:tufAImported
    Synonyms:tufUniRule annotation
    Ordered Locus Names:EKO11_0406Imported
    ORF Names:KO11_06105Imported
    OrganismiEscherichia coli (strain ATCC 55124 / KO11)Imported
    Taxonomic identifieri595495 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000002250: Chromosome, UP000010089: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Structurei

    3D structure databases

    SMRiE8Y1L0. Positions 9-393.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.UniRule annotation
    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

    Phylogenomic databases

    KOiK02358.
    OMAiERKPHIN.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00118_B. EF_Tu_B.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004541. Transl_elong_EFTu/EF1A_bac/org.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view]
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS00301. EFACTOR_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E8Y1L0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKEKFERTK PHVNVGTIGH VDHGKTTLTA AITTVLAKTY GGAARAFDQI    50
    DNAPEEKARG ITINTSHVEY DTPTRHYAHV DCPGHADYVK NMITGAAQMD 100
    GAILVVAATD GPMPQTREHI LLGRQVGVPY IIVFLNKCDM VDDEELLELV 150
    EMEVRELLSQ YDFPGDDTPI VRGSALKALE GDAEWEAKIL ELAGFLDSYI 200
    PEPERAIDKP FLLPIEDVFS ISGRGTVVTG RVERGIIKVG EEVEIVGIKE 250
    TQKSTCTGVE MFRKLLDEGR AGENVGVLLR GIKREEIERG QVLAKPGTIK 300
    PHTKFESEVY ILSKDEGGRH TPFFKGYRPQ FYFRTTDVTG TIELPEGVEM 350
    VMPGDNIKMV VTLIHPIAMD DGLRFAIREG GRTVGAGVVA KVLG 394
    Length:394
    Mass (Da):43,284
    Last modified:April 5, 2011 - v1
    Checksum:i731A60255F43358F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002516 Genomic DNA. Translation: ADX49064.1.
    CP002970 Genomic DNA. Translation: AFH16206.1.
    RefSeqiYP_005276128.1. NC_016902.1.
    YP_006163558.1. NC_017660.1.

    Genome annotation databases

    EnsemblBacteriaiADX49064; ADX49064; EKO11_0406.
    AFH16206; AFH16206; KO11_06105.
    GeneIDi11775419.
    12761404.
    KEGGiekf:KO11_06105.
    eko:EKO11_0406.
    PATRICi48640612. VBIEscCol13896_0411.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002516 Genomic DNA. Translation: ADX49064.1 .
    CP002970 Genomic DNA. Translation: AFH16206.1 .
    RefSeqi YP_005276128.1. NC_016902.1.
    YP_006163558.1. NC_017660.1.

    3D structure databases

    SMRi E8Y1L0. Positions 9-393.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADX49064 ; ADX49064 ; EKO11_0406 .
    AFH16206 ; AFH16206 ; KO11_06105 .
    GeneIDi 11775419.
    12761404.
    KEGGi ekf:KO11_06105.
    eko:EKO11_0406.
    PATRICi 48640612. VBIEscCol13896_0411.

    Phylogenomic databases

    KOi K02358.
    OMAi ERKPHIN.

    Enzyme and pathway databases

    BioCyci ECOL595495:GI1Q-408-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_00118_B. EF_Tu_B.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004541. Transl_elong_EFTu/EF1A_bac/org.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view ]
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00485. EF-Tu. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS00301. EFACTOR_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome of Escherichia coli KO11."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Munk A.C., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Keating D., Landick R., Woyke T.
      Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 55124 / KO11Imported and KO11Imported.
    2. "Optical mapping and sequencing of the Escherichia coli KO11 genome reveal extensive chromosomal rearrangements, and multiple tandem copies of the Zymomonas mobilis pdc and adhB genes."
      Turner P.C., Yomano L.P., Jarboe L.R., York S.W., Baggett C.L., Moritz B.E., Zentz E.B., Shanmugam K.T., Ingram L.O.
      J. Ind. Microbiol. Biotechnol. 39:629-639(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KO11FLImported.

    Entry informationi

    Entry nameiE8Y1L0_ECOKO
    AccessioniPrimary (citable) accession number: E8Y1L0
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 5, 2011
    Last sequence update: April 5, 2011
    Last modified: October 1, 2014
    This is version 31 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3