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E8XTP7 (E8XTP7_RAHSY) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NAD-dependent malic enzyme HAMAP-Rule MF_01619
Short name=NAD-ME HAMAP-Rule MF_01619
EC=1.1.1.38 HAMAP-Rule MF_01619
Gene names
Name:maeA HAMAP-Rule MF_01619
Ordered Locus Names:Rahaq_1422 EMBL ADW73045.1
OrganismRahnella sp. (strain Y9602) [Complete proteome] [HAMAP] EMBL ADW73045.1
Taxonomic identifier741091 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Rahnella

Protein attributes

Sequence length565 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP-Rule MF_01619 SAAS SAAS012301

Oxaloacetate = pyruvate + CO2. HAMAP-Rule MF_01619 SAAS SAAS012301

Cofactor

Divalent metal cations. Prefers magnesium or manganese By similarity. HAMAP-Rule MF_01619 PIRSR PIRSR000106-3 SAAS SAAS012301

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01619 SAAS SAAS012301

Sequence similarities

Belongs to the malic enzymes family. HAMAP-Rule MF_01619 RuleBase RU003427

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1041Proton donor By similarity HAMAP-Rule MF_01619
Active site1751Proton acceptor By similarity HAMAP-Rule MF_01619
Metal binding2461Divalent metal cation By similarity HAMAP-Rule MF_01619 PIRSR PIRSR000106-3
Metal binding2471Divalent metal cation By similarity HAMAP-Rule MF_01619 PIRSR PIRSR000106-3
Metal binding2701Divalent metal cation By similarity HAMAP-Rule MF_01619 PIRSR PIRSR000106-3
Binding site1571NAD By similarity HAMAP-Rule MF_01619
Binding site2701NAD By similarity HAMAP-Rule MF_01619
Binding site4181NAD By similarity HAMAP-Rule MF_01619
Site2701Important for activity By similarity HAMAP-Rule MF_01619

Sequences

Sequence LengthMass (Da)Tools
E8XTP7 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: 5B48E89F606ECEBB

FASTA56561,825
        10         20         30         40         50         60 
MELEYESKRP LYIPHAGPIL LEFPLLNKGS AFTEEERSNF NLHGLLPEAV ETIEEQADRA 

        70         80         90        100        110        120 
YRQFQNFKTD IDKHIYLRNI QDTNETLFYR LLDNHLSEMM PVIYTPTVGE ACEHFSDIYR 

       130        140        150        160        170        180 
RARGLFISYP NRAHIDDMLQ NATKQHVKVV VVTDGERILG LGDQGIGGMG IPIGKLSLYT 

       190        200        210        220        230        240 
ACGGISPAYT LPVVLDAGTN NPQLLNDPLY MGWRHPRITG EEYEAFVDQF IQAVKIRWPN 

       250        260        270        280        290        300 
VLLQFEDFAQ KNAMPILERY RDELCCFNDD IQGTASVALG SLIAASKAAG GKLSDQTVAF 

       310        320        330        340        350        360 
LGAGSAGCGI AEQIVAQMKS EGLSEEEARA KVFMVDRFGL LTDKLPNLLD FQSKLVQKSS 

       370        380        390        400        410        420 
ALASWGMESD SVSLLDVVRN ARPTVLIGVS GQPGLFTEEI IREMHKHCAR PVVMPLSNPT 

       430        440        450        460        470        480 
SRVEATPADI LNWTDGAALV ATGSPFAPVS HKGKLYPIAQ CNNSFIFPGI GLGVIASGAT 

       490        500        510        520        530        540 
RVTDGMLMAA SRTLAECSPL ATEGTGSLLP DVDDIQGVSK CIAMAVGKAA QLQGVAMVTS 

       550        560 
EDSLSKAIEH NFWAPQYRSY KRTSF

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References

[1]"Complete sequence of chromosome of Rahnella sp. Y9602."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Lu M., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Sobecky P.A., Martinez R.J., Woyke T.
Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Y9602.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002505 Genomic DNA. Translation: ADW73045.1.
RefSeqYP_004212172.1. NC_015061.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADW73045; ADW73045; Rahaq_1422.
GeneID10212423.
KEGGrah:Rahaq_1422.
PATRIC47401043. VBIRahSp167412_1426.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000042487.
KOK00027.

Enzyme and pathway databases

BioCycRSP741091:GHHP-2209-MONOMER.

Family and domain databases

Gene3D3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
HAMAPMF_01619. NAD_malic_enz.
InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
IPR023667. NAD_malic_enz_proteobac.
[Graphical view]
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFPIRSF000106. ME. 1 hit.
PRINTSPR00072. MALOXRDTASE.
SMARTSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE8XTP7_RAHSY
AccessionPrimary (citable) accession number: E8XTP7
Entry history
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: May 1, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info