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E8XMF0

- APHA_RAHSY

UniProt

E8XMF0 - APHA_RAHSY

Protein

Class B acid phosphatase

Gene

aphA

Organism
Rahnella sp. (strain Y9602)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 22 (01 Oct 2014)
      Sequence version 2 (25 Jan 2012)
      Previous versions | rss
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    Functioni

    Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.By similarity

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.By similarity

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei69 – 691NucleophileBy similarity
    Metal bindingi69 – 691MagnesiumBy similarity
    Active sitei71 – 711Proton donorBy similarity
    Metal bindingi71 – 711Magnesium; via carbonyl oxygenBy similarity
    Binding sitei177 – 1771SubstrateBy similarity
    Metal bindingi192 – 1921MagnesiumBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciRSP741091:GHHP-3159-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Class B acid phosphataseBy similarity (EC:3.1.3.2ImportedBy similarity)
    Short name:
    CBAPBy similarity
    Gene namesi
    Name:aphABy similarity
    Ordered Locus Names:Rahaq_3102
    OrganismiRahnella sp. (strain Y9602)
    Taxonomic identifieri741091 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeRahnella
    ProteomesiUP000007257: Chromosome

    Subcellular locationi

    Periplasm By similarity

    GO - Cellular componenti

    1. outer membrane-bounded periplasmic space Source: InterPro

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 237214Class B acid phosphataseSequence AnalysisPRO_5000703176Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliE8XMF0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni137 – 1382Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class B bacterial acid phosphatase family.By similarity

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000270623.
    KOiK03788.

    Family and domain databases

    Gene3Di3.40.50.1000. 1 hit.
    InterProiIPR005519. Acid_phosphat_B.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view]
    PfamiPF03767. Acid_phosphat_B. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01672. AphA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    E8XMF0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKTPLALSA VFLLLSLNQS AFAKEPAPAP LWPGVNVAQL AQQSPVHWVS    50
    VAQIENSLNG RPPIAVGFDI DDTVLFSSPG FYRGQVEFSP GKQDYLKNPK 100
    FWEKMNNGWD DFSMPKEVAK SLIAMHLKRG DSIYFVTGRS ETKTETVTKT 150
    LQNDFQIPQD KVNAVIFAGD KAGQNTKTQW LKDKQIKVFY GDSDNDITAA 200
    QSVSARGIRV LRASNSSYKP LPQAGAFGEE VIVNSEY 237
    Length:237
    Mass (Da):26,216
    Last modified:January 25, 2012 - v2
    Checksum:i8F9565C278739505
    GO

    Sequence cautioni

    The sequence ADW74696.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002505 Genomic DNA. Translation: ADW74696.1. Different initiation.
    RefSeqiWP_013576392.1. NC_015061.1.
    YP_004213823.1. NC_015061.1.

    Genome annotation databases

    EnsemblBacteriaiADW74696; ADW74696; Rahaq_3102.
    GeneIDi10214117.
    KEGGirah:Rahaq_3102.
    PATRICi47404400. VBIRahSp167412_3086.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002505 Genomic DNA. Translation: ADW74696.1 . Different initiation.
    RefSeqi WP_013576392.1. NC_015061.1.
    YP_004213823.1. NC_015061.1.

    3D structure databases

    ProteinModelPortali E8XMF0.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADW74696 ; ADW74696 ; Rahaq_3102 .
    GeneIDi 10214117.
    KEGGi rah:Rahaq_3102.
    PATRICi 47404400. VBIRahSp167412_3086.

    Phylogenomic databases

    HOGENOMi HOG000270623.
    KOi K03788.

    Enzyme and pathway databases

    BioCyci RSP741091:GHHP-3159-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1000. 1 hit.
    InterProi IPR005519. Acid_phosphat_B.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view ]
    Pfami PF03767. Acid_phosphat_B. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR01672. AphA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome of Rahnella sp. Y9602."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Lu M., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Sobecky P.A., Martinez R.J., Woyke T.
      Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Y9602Imported.

    Entry informationi

    Entry nameiAPHA_RAHSY
    AccessioniPrimary (citable) accession number: E8XMF0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: January 25, 2012
    Last modified: October 1, 2014
    This is version 22 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3