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E8XMF0

- APHA_RAHSY

UniProt

E8XMF0 - APHA_RAHSY

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Protein

Class B acid phosphatase

Gene

aphA

Organism
Rahnella sp. (strain Y9602)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei69 – 691NucleophileBy similarity
Metal bindingi69 – 691MagnesiumBy similarity
Active sitei71 – 711Proton donorBy similarity
Metal bindingi71 – 711Magnesium; via carbonyl oxygenBy similarity
Binding sitei177 – 1771SubstrateBy similarity
Metal bindingi192 – 1921MagnesiumBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciRSP741091:GHHP-3159-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphataseBy similarity (EC:3.1.3.2ImportedBy similarity)
Short name:
CBAPBy similarity
Gene namesi
Name:aphABy similarity
Ordered Locus Names:Rahaq_3102
OrganismiRahnella sp. (strain Y9602)
Taxonomic identifieri741091 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeRahnella
ProteomesiUP000007257: Chromosome

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 237214Class B acid phosphataseSequence AnalysisPRO_5000703176Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliE8XMF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1382Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class B bacterial acid phosphatase family.By similarity

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000270623.
KOiK03788.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E8XMF0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRKTPLALSA VFLLLSLNQS AFAKEPAPAP LWPGVNVAQL AQQSPVHWVS
60 70 80 90 100
VAQIENSLNG RPPIAVGFDI DDTVLFSSPG FYRGQVEFSP GKQDYLKNPK
110 120 130 140 150
FWEKMNNGWD DFSMPKEVAK SLIAMHLKRG DSIYFVTGRS ETKTETVTKT
160 170 180 190 200
LQNDFQIPQD KVNAVIFAGD KAGQNTKTQW LKDKQIKVFY GDSDNDITAA
210 220 230
QSVSARGIRV LRASNSSYKP LPQAGAFGEE VIVNSEY
Length:237
Mass (Da):26,216
Last modified:January 25, 2012 - v2
Checksum:i8F9565C278739505
GO

Sequence cautioni

The sequence ADW74696.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002505 Genomic DNA. Translation: ADW74696.1. Different initiation.
RefSeqiWP_013576392.1. NC_015061.1.
YP_004213823.1. NC_015061.1.

Genome annotation databases

EnsemblBacteriaiADW74696; ADW74696; Rahaq_3102.
GeneIDi10214117.
KEGGirah:Rahaq_3102.
PATRICi47404400. VBIRahSp167412_3086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002505 Genomic DNA. Translation: ADW74696.1 . Different initiation.
RefSeqi WP_013576392.1. NC_015061.1.
YP_004213823.1. NC_015061.1.

3D structure databases

ProteinModelPortali E8XMF0.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADW74696 ; ADW74696 ; Rahaq_3102 .
GeneIDi 10214117.
KEGGi rah:Rahaq_3102.
PATRICi 47404400. VBIRahSp167412_3086.

Phylogenomic databases

HOGENOMi HOG000270623.
KOi K03788.

Enzyme and pathway databases

BioCyci RSP741091:GHHP-3159-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view ]
Pfami PF03767. Acid_phosphat_B. 1 hit.
[Graphical view ]
PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01672. AphA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Rahnella sp. Y9602."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Lu M., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Sobecky P.A., Martinez R.J., Woyke T.
    Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Y9602Imported.

Entry informationi

Entry nameiAPHA_RAHSY
AccessioniPrimary (citable) accession number: E8XMF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 25, 2012
Last modified: November 26, 2014
This is version 23 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3