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Protein

Class B acid phosphatase

Gene

aphA

Organism
Rahnella sp. (strain Y9602)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei69NucleophileBy similarity1
Metal bindingi69MagnesiumBy similarity1
Active sitei71Proton donorBy similarity1
Metal bindingi71Magnesium; via carbonyl oxygenBy similarity1
Binding sitei177SubstrateBy similarity1
Metal bindingi192MagnesiumBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphataseBy similarity (EC:3.1.3.2By similarityImported)
Short name:
CBAPBy similarity
Gene namesi
Name:aphABy similarity
Ordered Locus Names:Rahaq_3102
OrganismiRahnella sp. (strain Y9602)
Taxonomic identifieri741091 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeRahnella
Proteomesi
  • UP000007257 Componenti: Chromosome

Subcellular locationi

  • Periplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_500070317624 – 237Class B acid phosphataseSequence analysisAdd BLAST214

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi741091.Rahaq_3102.

Structurei

3D structure databases

ProteinModelPortaliE8XMF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 138Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the class B bacterial acid phosphatase family.By similarity

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
HOGENOMiHOG000270623.
KOiK03788.
OrthoDBiPOG091H06BK.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E8XMF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKTPLALSA VFLLLSLNQS AFAKEPAPAP LWPGVNVAQL AQQSPVHWVS
60 70 80 90 100
VAQIENSLNG RPPIAVGFDI DDTVLFSSPG FYRGQVEFSP GKQDYLKNPK
110 120 130 140 150
FWEKMNNGWD DFSMPKEVAK SLIAMHLKRG DSIYFVTGRS ETKTETVTKT
160 170 180 190 200
LQNDFQIPQD KVNAVIFAGD KAGQNTKTQW LKDKQIKVFY GDSDNDITAA
210 220 230
QSVSARGIRV LRASNSSYKP LPQAGAFGEE VIVNSEY
Length:237
Mass (Da):26,216
Last modified:January 25, 2012 - v2
Checksum:i8F9565C278739505
GO

Sequence cautioni

The sequence ADW74696 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002505 Genomic DNA. Translation: ADW74696.1. Different initiation.
RefSeqiWP_015690236.1. NC_015061.1.

Genome annotation databases

EnsemblBacteriaiADW74696; ADW74696; Rahaq_3102.
KEGGirah:Rahaq_3102.
PATRICi47404400. VBIRahSp167412_3086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002505 Genomic DNA. Translation: ADW74696.1. Different initiation.
RefSeqiWP_015690236.1. NC_015061.1.

3D structure databases

ProteinModelPortaliE8XMF0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi741091.Rahaq_3102.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADW74696; ADW74696; Rahaq_3102.
KEGGirah:Rahaq_3102.
PATRICi47404400. VBIRahSp167412_3086.

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
HOGENOMiHOG000270623.
KOiK03788.
OrthoDBiPOG091H06BK.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPHA_RAHSY
AccessioniPrimary (citable) accession number: E8XMF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 25, 2012
Last modified: November 2, 2016
This is version 31 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.