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E8XMF0 (APHA_RAHSY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Class B acid phosphatase

Short name=CBAP
EC=3.1.3.2
Gene names
Name:aphA
Ordered Locus Names:Rahaq_3102
OrganismRahnella sp. (strain Y9602) [Complete proteome] [HAMAP]
Taxonomic identifier741091 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeRahnella

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the class B bacterial acid phosphatase family.

Sequence caution

The sequence ADW74696.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentouter membrane-bounded periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 237214Class B acid phosphatase
PRO_5000703176

Regions

Region137 – 1382Substrate binding By similarity

Sites

Active site691Nucleophile By similarity
Active site711Proton donor By similarity
Metal binding691Magnesium By similarity
Metal binding711Magnesium; via carbonyl oxygen By similarity
Metal binding1921Magnesium By similarity
Binding site1771Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
E8XMF0 [UniParc].

Last modified January 25, 2012. Version 2.
Checksum: 8F9565C278739505

FASTA23726,216
        10         20         30         40         50         60 
MRKTPLALSA VFLLLSLNQS AFAKEPAPAP LWPGVNVAQL AQQSPVHWVS VAQIENSLNG 

        70         80         90        100        110        120 
RPPIAVGFDI DDTVLFSSPG FYRGQVEFSP GKQDYLKNPK FWEKMNNGWD DFSMPKEVAK 

       130        140        150        160        170        180 
SLIAMHLKRG DSIYFVTGRS ETKTETVTKT LQNDFQIPQD KVNAVIFAGD KAGQNTKTQW 

       190        200        210        220        230 
LKDKQIKVFY GDSDNDITAA QSVSARGIRV LRASNSSYKP LPQAGAFGEE VIVNSEY 

« Hide

References

[1]"Complete sequence of chromosome of Rahnella sp. Y9602."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Lu M., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Sobecky P.A., Martinez R.J., Woyke T.
Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Y9602.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002505 Genomic DNA. Translation: ADW74696.1. Different initiation.
RefSeqYP_004213823.1. NC_015061.1.

3D structure databases

ProteinModelPortalE8XMF0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADW74696; ADW74696; Rahaq_3102.
GeneID10214117.
KEGGrah:Rahaq_3102.
PATRIC47404400. VBIRahSp167412_3086.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000270623.
KOK03788.

Enzyme and pathway databases

BioCycRSP741091:GHHP-3159-MONOMER.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01672. AphA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAPHA_RAHSY
AccessionPrimary (citable) accession number: E8XMF0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 25, 2012
Last modified: April 16, 2014
This is version 20 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families