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E8X9L1 (E8X9L1_SALT4) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Lysine--tRNA ligase RuleBase RU000336 HAMAP-Rule MF_00252
EC=6.1.1.6 RuleBase RU000336 HAMAP-Rule MF_00252
Alternative name(s):
Lysyl-tRNA synthetase HAMAP-Rule MF_00252
Gene names
Name:lysS HAMAP-Rule MF_00252 EMBL ADX18826.1
Ordered Locus Names:STM474_3187 EMBL ADX18826.1
OrganismSalmonella typhimurium (strain 4/74) [Complete proteome] [HAMAP] EMBL ADX18826.1
Taxonomic identifier909946 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella ›

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). RuleBase RU000336 HAMAP-Rule MF_00252 SAAS SAAS002313

Cofactor

Binds 3 magnesium ions per subunit By similarity. RuleBase RU000336 HAMAP-Rule MF_00252

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00252 SAAS SAAS002313

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00252.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. HAMAP-Rule MF_00252

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding4151Magnesium 1 By similarity HAMAP-Rule MF_00252
Metal binding4221Magnesium 1 By similarity HAMAP-Rule MF_00252
Metal binding4221Magnesium 2 By similarity HAMAP-Rule MF_00252

Sequences

Sequence LengthMass (Da)Tools
E8X9L1 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: 48EBE562C708F96F

FASTA50557,575
        10         20         30         40         50         60 
MSEQNAQGAD EVVDLNNEMK ARREKLAALR EQGIPFPNDF RRDRTSDQLH AEFDAKEAEE 

        70         80         90        100        110        120 
LEALNIEVSV AGRMMTRRIM GKASFVTLQD VGGRIQLYVA RDDLPEGVYN EQFKKWDLGD 

       130        140        150        160        170        180 
ILGAKGKLFK TKTGELSIHC TELRLLTKAL RPLPDKFHGL QDQEARYRQR YLDLISNDES 

       190        200        210        220        230        240 
RNTFKTRSKI LAGIRQFMVA RGFMEVETPM MQVIPGGASA RPFITHHNAL DLDMYLRIAP 

       250        260        270        280        290        300 
ELYLKRLVVG GFERVFEINR NFRNEGISVR HNPEFTMMEL YMAYADYKDL IELTESLFRT 

       310        320        330        340        350        360 
LAQDVLGTTQ VPYGDEVFDF GKPFEKLTMR EAIKKYRPET DMADLDNFDS AKAIAESIGI 

       370        380        390        400        410        420 
HVEKSWGLGR IVTEIFDEVA EAHLIQPTFI TEYPAEVSPL ARRNDVNPEI TDRFEFFIGG 

       430        440        450        460        470        480 
REIGNGFSEL NDAEDQAQRF LDQVNAKAAG DDEAMFYDED YVTALEHGLP PTAGLGIGID 

       490        500 
RMVMLFTNSH TIRDVILFPA MRPVK

« Hide

References

[1]"Genome sequences of Salmonella enterica serovar typhimurium, Choleraesuis, Dublin, and Gallinarum strains of well- defined virulence in food-producing animals."
Richardson E.J., Limaye B., Inamdar H., Datta A., Manjari K.S., Pullinger G.D., Thomson N.R., Joshi R.R., Watson M., Stevens M.P.
J. Bacteriol. 193:3162-3163(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 4/74.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002487 Genomic DNA. Translation: ADX18826.1.
RefSeqYP_005244025.1. NC_016857.1.

3D structure databases

ProteinModelPortalE8X9L1.
SMRE8X9L1. Positions 12-503.
ModBaseSearch...

Proteomic databases

PRIDEE8X9L1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADX18826; ADX18826; STM474_3187.
GeneID11750717.
KEGGseb:STM474_3187.
PATRIC47198066. VBISalEnt171491_3262.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK04567.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
HAMAPMF_00252. Lys_tRNA_synth_class2.
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA-helicase.
[Graphical view]
PANTHERPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF4. PTHR22594:SF4. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]
PRINTSPR00982. TRNASYNTHLYS.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMsTIGR00499. lysS_bact. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE8X9L1_SALT4
AccessionPrimary (citable) accession number: E8X9L1
Entry history
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: May 1, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info