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E8WWT4 (E8WWT4_ACISM) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:AciX9_0340 EMBL ADW67412.1
OrganismAcidobacterium sp. (strain MP5ACTX9) [Complete proteome] [HAMAP]
Taxonomic identifier696844 [NCBI]
Taxonomic lineageBacteriaAcidobacteriaAcidobacterialesAcidobacteriaceaeAcidobacterium

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain137 – 355219ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding170 – 22556ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3041Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3221Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3221Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3241Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E8WWT4 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: 45BF0EC422B43167

FASTA37239,652
        10         20         30         40         50         60 
MAKKKLRVGV LFGGRSGEHE VSLLSANSIL KAIDRKKYEV VPIGITKQGR WLGGDGAKGL 

        70         80         90        100        110        120 
LTGDSGLTLS ASAQVTETGL IEQSGGVIGA LDVIFPVLHG TFGEDGTIQG MLELADLAYV 

       130        140        150        160        170        180 
GSGVLGSAAG MDKDVMKKLF AAAGLPQTPY LSVLRSEWKA DAKGCRKRIE KALEYPLFVK 

       190        200        210        220        230        240 
PANLGSSVGI SKVHGRDELA AAMDLAAGFD RKLVVEQGVG GPGAKPRELE VAVLGNETLE 

       250        260        270        280        290        300 
ASVVGEIVPA KEFYDYEAKY QMNGPDESVC IIPAKLSASE QKQMRAMAME AFRACDCSGL 

       310        320        330        340        350        360 
ARVDFLMEPA AKGKKAKFYL NEINTMPGFT SISMYPKLWG ASGVGYTELI DRLIVLAQER 

       370 
HAEKMATAFA RE 

« Hide

References

[1]"Complete sequence of chromosome of Acidobacterium sp. MP5ACTX9."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Teshima H., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Rawat S.R., Mannisto M., Haggblom M.M., Woyke T.
Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MP5ACTX9.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002480 Genomic DNA. Translation: ADW67412.1.
RefSeqYP_004216192.1. NC_015064.1.

3D structure databases

ProteinModelPortalE8WWT4.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADW67412; ADW67412; AciX9_0340.
GeneID10202318.
KEGGacm:AciX9_0340.
PATRIC46818101. VBIAciSp155132_0977.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011593.
KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE8WWT4_ACISM
AccessionPrimary (citable) accession number: E8WWT4
Entry history
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: February 19, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)