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E8WDE5 (E8WDE5_STRFA) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable DNA ligase HAMAP-Rule MF_00407
EC=6.5.1.1 HAMAP-Rule MF_00407
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] HAMAP-Rule MF_00407
Gene names
Name:lig HAMAP-Rule MF_00407
Ordered Locus Names:Sfla_5642
OrganismStreptomyces flavogriseus (strain ATCC 33331 / DSM 40990 / IAF-45CD) [Complete proteome] [HAMAP] EMBL ADW07037.1
Taxonomic identifier591167 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces ›

Protein attributes

Sequence length511 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407 RuleBase RU000617

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family. HAMAP-Rule MF_00407 RuleBase RU004196

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2101N6-AMP-lysine intermediate By similarity HAMAP-Rule MF_00407
Binding site2081ATP By similarity HAMAP-Rule MF_00407
Binding site2151ATP By similarity HAMAP-Rule MF_00407
Binding site2301ATP By similarity HAMAP-Rule MF_00407
Binding site2591ATP By similarity HAMAP-Rule MF_00407
Binding site2991ATP By similarity HAMAP-Rule MF_00407
Binding site3771ATP By similarity HAMAP-Rule MF_00407
Binding site3831ATP By similarity HAMAP-Rule MF_00407

Sequences

Sequence LengthMass (Da)Tools
E8WDE5 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: 7847AD1DEF20A351

FASTA51154,804
        10         20         30         40         50         60 
MLLARIALVS LEVAATSART RKTALLAALF RDAEPDDVPV VIPYLAGRLP QGRLGIGWST 

        70         80         90        100        110        120 
LRVPVTPAGE PTLTVAGTDA ELSAIGALGG PGSQAARKER LTRLLGAATA QEQHFLRGLL 

       130        140        150        160        170        180 
TGEVRQGALD AVAVDALADA AGAPRDEVRR AVMLAGSLGP VARTLLAEGP GALASFRLTV 

       190        200        210        220        230        240 
GRPVQPMLAH TSRSVTEAID RLGPCVVEEK LDGIRVQVHR DGPQVRVYTR ALDDITDRLP 

       250        260        270        280        290        300 
EVAAAVTRLR AERFILDGEA IALGADGRPR PFQETAGRVG SRRDVATAAG AVPVVPVFFD 

       310        320        330        340        350        360 
VLSVDGRDLL DLPLSERHRD LASLVPEEMR VRRVAVEYPA DERRRAAAVA FSEDTLARGH 

       370        380        390        400        410        420 
EGVVVKGTAA PYSAGRRGAS WLKVKPVHTL DLVVLAAEWG HGRRTGRLSN LHLGARRDDG 

       430        440        450        460        470        480 
TFAMLGKTFK GLTDTMLSWQ TQRLRELATG DDGHVVTVRP ELVVEIAYDG LQSSTRYPEG 

       490        500        510 
VTLRFARVLR YREDKSAEEA DTVGTVLSRR G

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References

[1]"Complete sequence of chromosome of Streptomyces flavogriseus ATCC 33331."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Brumm P., Mead D., Woyke T.
Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33331 / DSM 40990 / IAF-45CD.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002475 Genomic DNA. Translation: ADW07037.1.
RefSeqYP_004926554.1. NC_016114.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADW07037; ADW07037; Sfla_5642.
GeneID11371055.
KEGGsfa:Sfla_5642.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01971.

Enzyme and pathway databases

BioCycSFLA591167:GI5Y-5856-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF117018. SSF117018. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE8WDE5_STRFA
AccessionPrimary (citable) accession number: E8WDE5
Entry history
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: May 1, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info