Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable DNA ligase

Gene

lig

Organism
Streptomyces pratensis (strain ATCC 33331 / IAF-45CD)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei208ATPUniRule annotation1
Active sitei210N6-AMP-lysine intermediateUniRule annotation1
Binding sitei215ATPUniRule annotation1
Binding sitei230ATPUniRule annotation1
Binding sitei259ATPUniRule annotation1
Binding sitei299ATPUniRule annotation1
Binding sitei377ATPUniRule annotation1
Binding sitei383ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:Sfla_5642Imported
OrganismiStreptomyces pratensis (strain ATCC 33331 / IAF-45CD)Imported
Taxonomic identifieri591167 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000002066 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi591167.Sfla_5642.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini299 – 417DNA_LIGASE_A3InterPro annotationAdd BLAST119

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RYT. Bacteria.
COG1793. LUCA.
KOiK10747.
OMAiRKHNIEE.
OrthoDBiPOG091H0BGA.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
HAMAPiMF_00407. DNA_ligase. 1 hit.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E8WDE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLARIALVS LEVAATSART RKTALLAALF RDAEPDDVPV VIPYLAGRLP
60 70 80 90 100
QGRLGIGWST LRVPVTPAGE PTLTVAGTDA ELSAIGALGG PGSQAARKER
110 120 130 140 150
LTRLLGAATA QEQHFLRGLL TGEVRQGALD AVAVDALADA AGAPRDEVRR
160 170 180 190 200
AVMLAGSLGP VARTLLAEGP GALASFRLTV GRPVQPMLAH TSRSVTEAID
210 220 230 240 250
RLGPCVVEEK LDGIRVQVHR DGPQVRVYTR ALDDITDRLP EVAAAVTRLR
260 270 280 290 300
AERFILDGEA IALGADGRPR PFQETAGRVG SRRDVATAAG AVPVVPVFFD
310 320 330 340 350
VLSVDGRDLL DLPLSERHRD LASLVPEEMR VRRVAVEYPA DERRRAAAVA
360 370 380 390 400
FSEDTLARGH EGVVVKGTAA PYSAGRRGAS WLKVKPVHTL DLVVLAAEWG
410 420 430 440 450
HGRRTGRLSN LHLGARRDDG TFAMLGKTFK GLTDTMLSWQ TQRLRELATG
460 470 480 490 500
DDGHVVTVRP ELVVEIAYDG LQSSTRYPEG VTLRFARVLR YREDKSAEEA
510
DTVGTVLSRR G
Length:511
Mass (Da):54,804
Last modified:April 5, 2011 - v1
Checksum:i7847AD1DEF20A351
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002475 Genomic DNA. Translation: ADW07037.1.
RefSeqiWP_014157639.1. NC_016114.1.

Genome annotation databases

EnsemblBacteriaiADW07037; ADW07037; Sfla_5642.
GeneIDi11371055.
KEGGisfa:Sfla_5642.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002475 Genomic DNA. Translation: ADW07037.1.
RefSeqiWP_014157639.1. NC_016114.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi591167.Sfla_5642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADW07037; ADW07037; Sfla_5642.
GeneIDi11371055.
KEGGisfa:Sfla_5642.

Phylogenomic databases

eggNOGiENOG4107RYT. Bacteria.
COG1793. LUCA.
KOiK10747.
OMAiRKHNIEE.
OrthoDBiPOG091H0BGA.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
HAMAPiMF_00407. DNA_ligase. 1 hit.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE8WDE5_STRFA
AccessioniPrimary (citable) accession number: E8WDE5
Entry historyi
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: November 30, 2016
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.