Elongation factor 4 - Streptomyces flavogriseus (strain ATCC 33331 / DSM 40990 / IAF-45CD)

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E8WB53 (E8WB53_STRFA) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 20. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Elongation factor 4 HAMAP-Rule MF_00071
Short name=EF-4 HAMAP-Rule MF_00071
EC=3.6.5.n1 HAMAP-Rule MF_00071

Alternative name(s):
Ribosomal back-translocase LepA HAMAP-Rule MF_00071

Gene names

Name:	lepA HAMAP-Rule MF_00071
Ordered Locus Names:	Sfla_4325

Organism

Streptomyces flavogriseus (strain ATCC 33331 / DSM 40990 / IAF-45CD) [Complete proteome] [HAMAP] EMBL ADW05733.1

Taxonomic identifier

591167 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces ›

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071
Catalytic activity	GTP + H₂O = GDP + phosphate. HAMAP-Rule MF_00071
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.
Sequence similarities	Belongs to the GTP-binding elongation factor family. LepA subfamily. HAMAP-Rule MF_00071

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00071
Cellular component	Cell membrane HAMAP-Rule MF_00071 Membrane
Ligand	GTP-binding RuleBase RU000323 HAMAP-Rule MF_00071 Nucleotide-binding
Molecular function	Hydrolase HAMAP-Rule MF_00071
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC positive regulation of translation Inferred from electronic annotation. Source: HAMAP translational elongation Inferred from electronic annotation. Source: GOC
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP ribosome binding Inferred from electronic annotation. Source: HAMAP translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

29 – 34

GTP By similarity HAMAP-Rule MF_00071

Nucleotide binding

150 – 153

GTP By similarity HAMAP-Rule MF_00071

Sequences

Sequence

Length

Mass (Da)

Tools

E8WB53 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: F2FCC730AFE5AE9A
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FASTA

624

68,575

        10         20         30         40         50         60 
MPATPTHVPE PSRTDPALIR NFCIIAHIDH GKSTLADRML QLTGVVDQRQ MRSQYLDRMD 

        70         80         90        100        110        120 
IERERGITIK SQAVRLPWAP TQGEGQGSTH ILNMIDTPGH VDFTYEVSRS LAACEGTVLL 

       130        140        150        160        170        180 
VDAAQGIEAQ TLANLYLAME NDLTIVPVLN KIDLPAAQPE KFSEELANLI GCQPEDVLKV 

       190        200        210        220        230        240 
SAKTGVGVDA LLDRVVRDVP APVGQADAPA RAMIFDSVYD AYRGVVTYVR VVDGQLNKRE 

       250        260        270        280        290        300 
RIRMMSTGAT HELLEIGVSS PEMTPADGIG VGEVGYIITG VKDVRQSKVG DTITSLNKGA 

       310        320        330        340        350        360 
TEALGGYKDP KPMVFSGLYP LDGSDYPDLR EALDKLQLND AALVYEPETS AALGFGFRVG 

       370        380        390        400        410        420 
FLGLLHLDVI RERLEREFGL ELIATAPNVV YRVEMEDGSE HTVTNPSEFP EGKIDKVHEP 

       430        440        450        460        470        480 
VVRATVLAPS EFIGAIMELC QNRRGTLIGM DYLSEDRVEI RYTLPLAEIV FDFFDQLKSK 

       490        500        510        520        530        540 
TRGYASLDYE PTGEQSAQLV KVDILLHGDK VDAFSAVTHK DKAYAYGVRL VAKLQKLIPR 

       550        560        570        580        590        600 
QNFEVPIQAA IGSRVIARET VRAIRKDVLA KCYGGDISRK RKLLEKQKEG KKRMKMVGNV 

       610        620 
EVPQDAFISV LSTDESAGEG KGKK

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References

[1]

"Complete sequence of chromosome of Streptomyces flavogriseus ATCC 33331."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Brumm P., Mead D., Woyke T.
Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33331 / DSM 40990 / IAF-45CD.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002475 Genomic DNA. Translation: ADW05733.1.
RefSeq	YP_004925250.1. NC_016114.1.
3D structure databases
ProteinModelPortal	E8WB53.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADW05733; ADW05733; Sfla_4325.
GeneID	11367321.
KEGG	sfa:Sfla_4325.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K03596.
Enzyme and pathway databases
BioCyc	SFLA591167:GI5Y-4519-MONOMER.
Family and domain databases
Gene3D	3.30.70.240. 1 hit.
HAMAP	MF_00071. LepA.
InterPro	IPR006297. EF-4. IPR000795. EF_GTP-bd_dom. IPR009022. EFG_III-V. IPR000640. EFG_V. IPR013842. LepA_GTP-bd_C. IPR005225. Small_GTP-bd_dom. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF40. PTHR23115:SF40. 1 hit.
Pfam	PF00679. EFG_C. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF06421. LepA_C. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR01393. lepA. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E8WB53_STRFA

Accession

Primary (citable) accession number: E8WB53

Entry history

Integrated into UniProtKB/TrEMBL:	April 5, 2011
Last sequence update:	April 5, 2011
Last modified:	May 1, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information