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E8VAB0 (E8VAB0_BACST) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL ADV95390.1
Ordered Locus Names:BSn5_13910 EMBL ADV95390.1
OrganismBacillus subtilis (strain BSn5) [Complete proteome] [HAMAP] EMBL ADV95390.1
Taxonomic identifier936156 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain140 – 345206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding173 – 22856ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2991Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3121Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3121Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3141Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E8VAB0 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: 60C45CC16BC55AF2

FASTA36139,800
        10         20         30         40         50         60 
MKTSLGLVYG GKSAEHNVSL QTALAVIKAL NTEKFDIHPI YITEKGEWVR GARLTEPVSN 

        70         80         90        100        110        120 
VKMLQFEQGG SAFSPAALNQ EMFPQEASQQ NEAIDVVFPL LHGPNGEDGT IQGMLELLNI 

       130        140        150        160        170        180 
PYVGNGVLAS SAGMDKVVMK HLFAQAGLAQ AKYAAFLKKD WTRSPKESCE QVEQELGYPC 

       190        200        210        220        230        240 
FVKPANLGSS VGISKCRNRE ELQKAFELAF QYDRKVVVEE GINGREIEIG VLGNDDPKCS 

       250        260        270        280        290        300 
VVGEIAPKTD FYDYKAKYED GDTDLMIPAI VTDEEYATIS DMAIKAFKAI DGSGLVRADF 

       310        320        330        340        350        360 
FLTADGEVLI NEVNTMPGFT PFSMFPLLWK EAGVEYADLI EQLVELAKER HAEKQLIKHT 


F 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Bacillus subtilis BSn5, an endophytic bacterium of Amorphophallus konjac with antimicrobial activity for the plant pathogen Erwinia carotovora subsp. carotovora."
Deng Y., Zhu Y., Wang P., Zhu L., Zheng J., Li R., Ruan L., Peng D., Sun M.
J. Bacteriol. 193:2070-2071(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BSn5 EMBL ADV95390.1.
[2]"Complete Genome Sequence of Bacillus Subtilis BSn5, a Strain of Plant-associated Bacterium with Antimicrobial Activity to Soil-borne Plant Pathogens."
Deng Y., Sun M.
Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: BSn5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002468 Genomic DNA. Translation: ADV95390.1.
RefSeqYP_004206417.1. NC_014976.1.

3D structure databases

ProteinModelPortalE8VAB0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADV95390; ADV95390; BSn5_13910.
GeneID10183480.
KEGGbsn:BSn5_13910.
PATRIC46872918. VBIBacSub180317_2839.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011593.
KOK01921.

Enzyme and pathway databases

BioCycBSUB936156:GHCY-2832-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE8VAB0_BACST
AccessionPrimary (citable) accession number: E8VAB0
Entry history
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: April 16, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)