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E8VAB0

- E8VAB0_BACST

UniProt

E8VAB0 - E8VAB0_BACST

Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Bacillus subtilis (strain BSn5)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 28 (01 Oct 2014)
      Sequence version 1 (05 Apr 2011)
      Previous versions | rss
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    Functioni

    Cell wall formation.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi299 – 2991Magnesium or manganese 1UniRule annotation
    Metal bindingi312 – 3121Magnesium or manganese 1UniRule annotation
    Metal bindingi312 – 3121Magnesium or manganese 2UniRule annotation
    Metal bindingi314 – 3141Magnesium or manganese 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi173 – 22856ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. D-alanine-D-alanine ligase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. manganese ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    2. regulation of cell shape Source: UniProtKB-KW

    Keywords - Molecular functioni

    LigaseUniRule annotationSAAS annotationImported

    Keywords - Biological processi

    Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSUB936156:GHCY-2832-MONOMER.
    UniPathwayiUPA00219.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
    Alternative name(s):
    D-Ala-D-Ala ligaseUniRule annotation
    D-alanylalanine synthetaseUniRule annotation
    Gene namesi
    Name:ddlUniRule annotationImported
    Ordered Locus Names:BSn5_13910Imported
    OrganismiBacillus subtilis (strain BSn5)Imported
    Taxonomic identifieri936156 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000007474: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliE8VAB0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini140 – 345206ATP-graspUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the D-alanine--D-alanine ligase family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation
    Contains ATP-grasp domain.SAAS annotation

    Phylogenomic databases

    HOGENOMiHOG000011593.
    KOiK01921.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 2 hits.
    3.40.50.20. 1 hit.
    HAMAPiMF_00047. Dala_Dala_lig.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000291. D-Ala_lig_Van_CS.
    IPR005905. D_ala_D_ala.
    IPR011095. Dala_Dala_lig_C.
    IPR011127. Dala_Dala_lig_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR23132. PTHR23132. 1 hit.
    PfamiPF07478. Dala_Dala_lig_C. 1 hit.
    PF01820. Dala_Dala_lig_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00843. DALA_DALA_LIGASE_1. 1 hit.
    PS00844. DALA_DALA_LIGASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E8VAB0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTSLGLVYG GKSAEHNVSL QTALAVIKAL NTEKFDIHPI YITEKGEWVR    50
    GARLTEPVSN VKMLQFEQGG SAFSPAALNQ EMFPQEASQQ NEAIDVVFPL 100
    LHGPNGEDGT IQGMLELLNI PYVGNGVLAS SAGMDKVVMK HLFAQAGLAQ 150
    AKYAAFLKKD WTRSPKESCE QVEQELGYPC FVKPANLGSS VGISKCRNRE 200
    ELQKAFELAF QYDRKVVVEE GINGREIEIG VLGNDDPKCS VVGEIAPKTD 250
    FYDYKAKYED GDTDLMIPAI VTDEEYATIS DMAIKAFKAI DGSGLVRADF 300
    FLTADGEVLI NEVNTMPGFT PFSMFPLLWK EAGVEYADLI EQLVELAKER 350
    HAEKQLIKHT F 361
    Length:361
    Mass (Da):39,800
    Last modified:April 5, 2011 - v1
    Checksum:i60C45CC16BC55AF2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002468 Genomic DNA. Translation: ADV95390.1.
    RefSeqiYP_004206417.1. NC_014976.1.

    Genome annotation databases

    EnsemblBacteriaiADV95390; ADV95390; BSn5_13910.
    GeneIDi10183480.
    KEGGibsn:BSn5_13910.
    PATRICi46872918. VBIBacSub180317_2839.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002468 Genomic DNA. Translation: ADV95390.1 .
    RefSeqi YP_004206417.1. NC_014976.1.

    3D structure databases

    ProteinModelPortali E8VAB0.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADV95390 ; ADV95390 ; BSn5_13910 .
    GeneIDi 10183480.
    KEGGi bsn:BSn5_13910.
    PATRICi 46872918. VBIBacSub180317_2839.

    Phylogenomic databases

    HOGENOMi HOG000011593.
    KOi K01921.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .
    BioCyci BSUB936156:GHCY-2832-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 2 hits.
    3.40.50.20. 1 hit.
    HAMAPi MF_00047. Dala_Dala_lig.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000291. D-Ala_lig_Van_CS.
    IPR005905. D_ala_D_ala.
    IPR011095. Dala_Dala_lig_C.
    IPR011127. Dala_Dala_lig_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR23132. PTHR23132. 1 hit.
    Pfami PF07478. Dala_Dala_lig_C. 1 hit.
    PF01820. Dala_Dala_lig_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01205. D_ala_D_alaTIGR. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00843. DALA_DALA_LIGASE_1. 1 hit.
    PS00844. DALA_DALA_LIGASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Bacillus subtilis BSn5, an endophytic bacterium of Amorphophallus konjac with antimicrobial activity to plant pathogen Erwinia carotovora subsp. carotovora."
      Deng Y., Zhu Y., Wang P., Zhu L., Zheng J., Li R., Ruan L., Peng D., Sun M.
      J. Bacteriol. 193:2070-2071(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BSn5Imported.
    2. "Complete Genome Sequence of Bacillus Subtilis BSn5, a Strain of Plant-associated Bacterium with Antimicrobial Activity to Soil-borne Plant Pathogens."
      Deng Y., Sun M.
      Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: BSn5.

    Entry informationi

    Entry nameiE8VAB0_BACST
    AccessioniPrimary (citable) accession number: E8VAB0
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 5, 2011
    Last sequence update: April 5, 2011
    Last modified: October 1, 2014
    This is version 28 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3