ID   E8V8K6_TERSS            Unreviewed;       443 AA.
AC   E8V8K6;
DT   05-APR-2011, integrated into UniProtKB/TrEMBL.
DT   05-APR-2011, sequence version 1.
DT   27-MAR-2024, entry version 57.
DE   SubName: Full=Alpha amylase catalytic region {ECO:0000313|EMBL:ADV82985.1};
GN   OrderedLocusNames=AciPR4_2183 {ECO:0000313|EMBL:ADV82985.1};
OS   Terriglobus saanensis (strain ATCC BAA-1853 / DSM 23119 / SP1PR4).
OC   Bacteria; Acidobacteriota; Terriglobia; Terriglobales; Acidobacteriaceae;
OC   Terriglobus.
OX   NCBI_TaxID=401053 {ECO:0000313|EMBL:ADV82985.1, ECO:0000313|Proteomes:UP000006844};
RN   [1] {ECO:0000313|EMBL:ADV82985.1, ECO:0000313|Proteomes:UP000006844}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC BAA-1853 / DSM 23119 / SP1PR4
RC   {ECO:0000313|Proteomes:UP000006844};
RX   PubMed=23450133; DOI=10.4056/sigs.3036810;
RA   Rawat S.R., Mannisto M.K., Starovoytov V., Goodwin L., Nolan M., Hauser L.,
RA   Land M., Davenport K.W., Woyke T., Haggblom M.M.;
RT   "Complete genome sequence of Terriglobus saanensis type strain SP1PR4(T),
RT   an Acidobacteria from tundra soil.";
RL   Stand. Genomic Sci. 7:59-69(2012).
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
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DR   EMBL; CP002467; ADV82985.1; -; Genomic_DNA.
DR   RefSeq; WP_013568718.1; NC_014963.1.
DR   AlphaFoldDB; E8V8K6; -.
DR   STRING; 401053.AciPR4_2183; -.
DR   KEGG; tsa:AciPR4_2183; -.
DR   eggNOG; COG0366; Bacteria.
DR   HOGENOM; CLU_024572_3_0_0; -.
DR   OrthoDB; 9805159at2; -.
DR   Proteomes; UP000006844; Chromosome.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11314; AmyAc_arch_bac_plant_AmyA; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR015237; Alpha-amylase_C_pro.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR   Pfam; PF09154; Alpha-amy_C_pro; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 2.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE   4: Predicted;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006844}.
FT   DOMAIN          2..346
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   REGION          408..427
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        408..423
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        192
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   ACT_SITE        224
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   BINDING         105
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         362
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ   SEQUENCE   443 AA;  50803 MW;  5083A126905D600E CRC64;
     MAVMMQAFYW DAPQKENKRG EWWNYLNSKI PELSAAGIDT MWLPPISKAA SPDSPGYDPY
     DYFDLGDFDQ KGAIKTLYGN SAELKQLLAS MREKHIGAIA DMVINHNSGA DEEEVNPLDG
     QKRWTKFNPK SGRFPRDWNC FHPSRYEEAI IEGEEFAGFP HLCHRNPSVY KHMFDYARMI
     IEELGFDGFR FDFVKGFGAW MIGILAKYKY ERNGEEVRPF VVGEYWSGPE DIDNWLERVR
     AVTEGQIAAF DFPLRYKLKD MCDTPNYDLR NLTDDGSVVS KRPFNAVTFV ENHDMGGNEI
     VNDKILAYSF ILVNEGYPCI FWYDYYNLEL ARPLTPNGID ALIDAHHRYA GGEACILHAD
     PDLYIMQRGG TEGQPGLIYV LNNLGDQWSG TSIKTKWHNQ KFKPLAWSGH DEAHPDERTT
     NENGEAEFPA PPRGYCIYVP IFE
//