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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Thermoanaerobacter brockii subsp. finnii (strain ATCC 43586 / DSM 3389 / AKO-1) (Thermoanaerobacter finnii)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation
  • Mn2+SAAS annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi299Magnesium or manganese 1UniRule annotation1
Metal bindingi313Magnesium or manganese 1UniRule annotation1
Metal bindingi313Magnesium or manganese 2UniRule annotation1
Metal bindingi315Magnesium or manganese 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotationImported
Biological processCell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandATP-bindingPROSITE-ProRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotationSAAS annotation (EC:6.3.2.4UniRule annotationSAAS annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:Thebr_0193Imported
OrganismiThermoanaerobacter brockii subsp. finnii (strain ATCC 43586 / DSM 3389 / AKO-1) (Thermoanaerobacter finnii)Imported
Taxonomic identifieri509193 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter
Proteomesi
  • UP000002062 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliE8UU11.
SMRiE8UU11.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 346ATP-graspInterPro annotationAdd BLAST206

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000011593.
KOiK01921.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
PfamiView protein in Pfam
PF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
PIRSFiPIRSF039102. Ddl/VanB. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

E8UU11-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKKIAVLF GGQSGEHEVS LMSAKSIINN LDKDKYEIYM VGITKKGEWY
60 70 80 90 100
LYRGDVGKIE TGEWEKEGIP AIMGASTKYR GIITFEDGEN GFYPIDVVFP
110 120 130 140 150
VLHGPNGEDG TIQGLLELLD MPYVGANVLS SALCMDKVFT KRIFKEAGLP
160 170 180 190 200
TPDFVVVYGK EIEDLEAIKK KIEHLGYPCF VKPANLGSSV GITKVHNEEE
210 220 230 240 250
LPGALKLAAK YDRKLLIERG IDAREIECSV LGNENPEASI AGEIVPSNEF
260 270 280 290 300
YDYNAKYFDG GKSLLLIPAP LPDEKMEEVR KLAIKAYKAL DLRGMARVDF
310 320 330 340 350
LMDRNTGTLY LNEVNTIPGF TKISMYPKLW ESSGKSYSTL LDELINLAVE

SHNEKCREW
Length:359
Mass (Da):40,083
Last modified:April 5, 2011 - v1
Checksum:iA8646128A86188AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002466 Genomic DNA. Translation: ADV78821.1.
RefSeqiWP_009052136.1. NC_014964.1.

Genome annotation databases

EnsemblBacteriaiADV78821; ADV78821; Thebr_0193.
KEGGitbo:Thebr_0193.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiE8UU11_THEBF
AccessioniPrimary (citable) accession number: E8UU11
Entry historyiIntegrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: June 7, 2017
This is version 48 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported